Rw0G008350

Zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00317
OG0001915
Physical Location & Seq
Forward (+)
31888 .. 32442
555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G008350.1

Sequence Viewer

Length: 426 bp
ATGAAGAAGTGTGAGCTGTGTGATTCTATGGCAAAGATGTACTGTGAATCAGATCAGGCTAGTCTGTGTTGGGATTGTGACATCAAGGTTCATGGTGCAAACTTCTTGGTGGCGAAGCATTTAAGGACTCTGCTCTGCCATGTCTGTCAATCTCTAACCCCATGGAATGCCTCTGGACCCAAGCTTGGTCATACTGTTTCAGTTTGTGAGAATTGTATGAACAGTTCTAACAAGGAGTCCACAAATGAAGAAGAAGAAGAACATGATGATGATGATCATGAAGAAAATAGCATTGGAGAAGATGATGAACATGATGGTGGTGAATGTTGCCATGAAAGCTTCTCAGAAACAAGATCATCATATCCATGTAAGCGCAGGCGCTACAATGCACTCTTGAATTCTCAAGCACGCAATTTCTTCACCTAA

Protein Analysis

141

Amino Acids

16.05

Weight (kDa)

4.9

Isoelectric Point (pI)

65.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 2 - 40 3e-07 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 397
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 1 cut(s) 185
AgsI TTSAA 1 cut(s) 397
AjuI GAANNNNNNNTTGG 2 cut(s) 276, 308
AluBI AGCT 3 cut(s) 16, 184, 339
AluI AGCT 3 cut(s) 16, 184, 339
ApoI RAATTY 1 cut(s) 397
AspLEI GCGC 2 cut(s) 375, 381
AspS9I GGNCC 1 cut(s) 176
AsuHPI GGTGA 2 cut(s) 332, 412
AvaII GGWCC 1 cut(s) 176
BccI CCATC 1 cut(s) 308
BclI TGATCA 1 cut(s) 274
BfaI CTAG 1 cut(s) 60
BfoI RGCGCY 1 cut(s) 382
Bme18I GGWCC 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 178
BpuEI CTTGAG 1 cut(s) 387
BsaBI GATNNNNATC 1 cut(s) 273
BsaJI CCNNGG 1 cut(s) 161
Bsc4I CCNNNNNNNGG 1 cut(s) 185
Bse8I GATNNNNATC 1 cut(s) 273
BseDI CCNNGG 1 cut(s) 161
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 1 cut(s) 185
BseMII CTCAG 1 cut(s) 357
BslI CCNNNNNNNGG 1 cut(s) 185
BsmI GAATGC 1 cut(s) 172
Bsp143I GATC 3 cut(s) 52, 274, 353
Bsp19I CCATGG 1 cut(s) 161
BspCNI CTCAG 1 cut(s) 356
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 1 cut(s) 178
BssECI CCNNGG 1 cut(s) 161
BssMI GATC 3 cut(s) 52, 274, 353
BssT1I CCWWGG 1 cut(s) 161
Bst4CI ACNGT 3 cut(s) 44, 196, 224
BstC8I GCNNGC 2 cut(s) 377, 409
BstDEI CTNAG 1 cut(s) 343
BstDSI CCRYGG 1 cut(s) 161
BstH2I RGCGCY 1 cut(s) 382
BstHHI GCGC 2 cut(s) 375, 381
BstKTI GATC 3 cut(s) 55, 277, 356
BstMBI GATC 3 cut(s) 52, 274, 353
BstMWI GCNNNNNNNGC 1 cut(s) 336
BtgI CCRYGG 1 cut(s) 161
Cac8I GCNNGC 2 cut(s) 377, 409
CciI TCATGA 1 cut(s) 277
CfoI GCGC 2 cut(s) 375, 381
Cfr13I GGNCC 1 cut(s) 176
Csp6I GTAC 1 cut(s) 40
CviAII CATG 8 cut(s) 92, 140, 162, 263, 278, 311, 332, 366
CviJI RGCY 4 cut(s) 16, 59, 184, 339
CviKI_1 RGCY 4 cut(s) 16, 59, 184, 339
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 1 cut(s) 343
DpnI GATC 3 cut(s) 54, 276, 355
DpnII GATC 3 cut(s) 52, 274, 353
Eco130I CCWWGG 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 176
EcoRI GAATTC 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 161
FaeI CATG 8 cut(s) 95, 143, 165, 266, 281, 314, 335, 369
FatI CATG 8 cut(s) 91, 139, 161, 262, 277, 310, 331, 365
FbaI TGATCA 1 cut(s) 274
FspBI CTAG 1 cut(s) 60
GlaI GCGC 2 cut(s) 374, 380
HaeII RGCGCY 1 cut(s) 382
HhaI GCGC 2 cut(s) 375, 381
Hin1II CATG 8 cut(s) 95, 143, 165, 266, 281, 314, 335, 369
Hin6I GCGC 2 cut(s) 373, 379
HinP1I GCGC 2 cut(s) 373, 379
HindIII AAGCTT 2 cut(s) 182, 337
HinfI GANTC 4 cut(s) 23, 47, 127, 236
HphI GGTGA 2 cut(s) 332, 412
Hpy166II GTNNAC 1 cut(s) 240
Hpy188I TCNGA 2 cut(s) 52, 346
Hpy188III TCNNGA 3 cut(s) 174, 278, 394
Hpy8I GTNNAC 1 cut(s) 240
HpyCH4III ACNGT 3 cut(s) 44, 196, 224
HpyCH4V TGCA 2 cut(s) 98, 389
HpyF10VI GCNNNNNNNGC 1 cut(s) 336
HpyF3I CTNAG 1 cut(s) 343
Hsp92II CATG 8 cut(s) 95, 143, 165, 266, 281, 314, 335, 369
HspAI GCGC 2 cut(s) 373, 379
Ksp22I TGATCA 1 cut(s) 274
Kzo9I GATC 3 cut(s) 52, 274, 353
LpnPI CCDG 3 cut(s) 41, 159, 361
MaeI CTAG 1 cut(s) 60
MaeIII GTNAC 1 cut(s) 77
MalI GATC 3 cut(s) 54, 276, 355
MboI GATC 3 cut(s) 52, 274, 353
MboII GAAGA 8 cut(s) 16, 260, 263, 266, 269, 293, 311, 409
MluCI AATT 3 cut(s) 211, 397, 412
MlyI GAGTC 2 cut(s) 121, 245
MnlI CCTC 1 cut(s) 181
MseI TTAA 1 cut(s) 122
MslI CAYNNNNRTG 3 cut(s) 267, 315, 364
Mva1269I GAATGC 1 cut(s) 172
MwoI GCNNNNNNNGC 1 cut(s) 336
NcoI CCATGG 1 cut(s) 161
NdeII GATC 3 cut(s) 52, 274, 353
NlaIII CATG 8 cut(s) 95, 143, 165, 266, 281, 314, 335, 369
NlaIV GGNNCC 1 cut(s) 178
NmuCI GTSAC 1 cut(s) 77
PagI TCATGA 1 cut(s) 277
PctI GAATGC 1 cut(s) 172
PfeI GAWTC 2 cut(s) 23, 47
PleI GAGTC 2 cut(s) 121, 244
PpsI GAGTC 2 cut(s) 121, 244
PspN4I GGNNCC 1 cut(s) 178
PspPI GGNCC 1 cut(s) 176
PsrI GAACNNNNNNTAC 2 cut(s) 208, 240
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
RseI CAYNNNNRTG 3 cut(s) 267, 315, 364
SaqAI TTAA 1 cut(s) 122
Sau3AI GATC 3 cut(s) 52, 274, 353
Sau96I GGNCC 1 cut(s) 176
SchI GAGTC 2 cut(s) 121, 245
SetI ASST 5 cut(s) 18, 90, 186, 341, 425
SinI GGWCC 1 cut(s) 176
SmiMI CAYNNNNRTG 3 cut(s) 267, 315, 364
SmlI CTYRAG 1 cut(s) 402
SmoI CTYRAG 1 cut(s) 402
Sse9I AATT 3 cut(s) 211, 397, 412
SspMI CTAG 1 cut(s) 60
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 3 cut(s) 44, 196, 224
TasI AATT 3 cut(s) 211, 397, 412
TatI WGTACW 1 cut(s) 39
TfiI GAWTC 2 cut(s) 23, 47
Tru1I TTAA 1 cut(s) 122
Tru9I TTAA 1 cut(s) 122
TseFI GTSAC 1 cut(s) 77
Tsp45I GTSAC 1 cut(s) 77
TspDTI ATGAA 7 cut(s) 17, 80, 233, 261, 294, 321, 348
VpaK11BI GGWCC 1 cut(s) 176
XapI RAATTY 1 cut(s) 397
XspI CTAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.