pycom10g25840

Zinc finger protein CONSTANS-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001915
Physical Location & Seq
Reverse (-)
27325966 .. 27326445
480 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g25840.1

Sequence Viewer

Length: 480 bp
ATGAAGAAGTGCGAGCTGTGTGACTCTGCAGCAAAAATGTACTGCGAATCCGATCAAGCTAGCCTCTGCTGGGACTGCGACGTCAAAGTTCACGGCGCAAACTTTCTGGTAGCTAAGCATTCAAGAACTCTGCTCTGCCATGTCTGCCAATCTCCGACCCCCTGGATTGGCTCCGGTCTCAAGCTCTTTCCAACTGTTTCCGTTTGCGAGAATTGTGTCAATAATAATGAAGGAAATCATCATCAAGACGAAGAAGAAGAAGAAGAAGACTACGAGGAAGAAGACGAGGACGAGGACGAGGACGAGGACGAGGAGCAGGATGATGGGGATAAAGATGATGATGATGACAACCAAGTTGTTCCATGGTCTTGTAAGCCTCCTCCGCCTGTGTCTTCAAGTTCTTCAAGCAGTAGGGACGAAGAATGTTCCAGTGGAAGCATGAGCATTTCCAGATCAACAACCAGAAGGGAACCCAGGTGA

Protein Analysis

160

Amino Acids

17.87

Weight (kDa)

4.18

Isoelectric Point (pI)

82.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 2 - 43 5.4e-08 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 80
AatII GACGTC 1 cut(s) 84
AciI CCGC 1 cut(s) 383
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 2 cut(s) 70, 167
AgsI TTSAA 3 cut(s) 123, 396, 405
AjnI CCWGG 2 cut(s) 161, 473
AluBI AGCT 4 cut(s) 16, 59, 113, 184
AluI AGCT 4 cut(s) 16, 59, 113, 184
Alw26I GTCTC 1 cut(s) 182
ApeKI GCWGC 1 cut(s) 29
AspLEI GCGC 1 cut(s) 98
AsuNHI GCTAGC 1 cut(s) 59
BbsI GAAGAC 3 cut(s) 273, 288, 384
BbvI GCAGC 1 cut(s) 41
BccI CCATC 1 cut(s) 317
BceAI ACGGC 1 cut(s) 109
BciT130I CCWGG 2 cut(s) 163, 475
BcoDI GTCTC 1 cut(s) 182
BfaI CTAG 1 cut(s) 60
BfmI CTRYAG 1 cut(s) 27
BisI GCNGC 1 cut(s) 30
BlpI GCTNAGC 1 cut(s) 114
BlsI GCNGC 1 cut(s) 31
Bme1390I CCNGG 2 cut(s) 163, 475
BmiI GGNNCC 2 cut(s) 172, 471
BmrFI CCNGG 2 cut(s) 163, 475
BmtI GCTAGC 1 cut(s) 63
BpiI GAAGAC 3 cut(s) 273, 288, 384
Bpu1102I GCTNAGC 1 cut(s) 114
BpuEI CTTGAG 1 cut(s) 164
BsaHI GRCGYC 1 cut(s) 81
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 3 cut(s) 161, 362, 473
BsaWI WCCGGW 1 cut(s) 173
Bsc4I CCNNNNNNNGG 2 cut(s) 70, 167
Bse1I ACTGG 1 cut(s) 429
BseBI CCWGG 2 cut(s) 163, 475
BseDI CCNNGG 3 cut(s) 161, 362, 473
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 2 cut(s) 70, 167
BseNI ACTGG 1 cut(s) 429
BseRI GAGGAG 2 cut(s) 326, 369
BseXI GCAGC 1 cut(s) 41
BseYI CCCAGC 1 cut(s) 69
BsiSI CCGG 1 cut(s) 174
BslFI GGGAC 2 cut(s) 86, 428
BslI CCNNNNNNNGG 2 cut(s) 70, 167
BsmAI GTCTC 1 cut(s) 182
BsmFI GGGAC 2 cut(s) 86, 428
BsmI GAATGC 1 cut(s) 118
Bso31I GGTCTC 1 cut(s) 182
Bsp143I GATC 2 cut(s) 52, 452
Bsp1720I GCTNAGC 1 cut(s) 114
Bsp19I CCATGG 1 cut(s) 362
BspACI CCGC 1 cut(s) 383
BspLI GGNNCC 2 cut(s) 172, 471
BspMAI CTGCAG 1 cut(s) 31
BspOI GCTAGC 1 cut(s) 63
BspTNI GGTCTC 1 cut(s) 182
BsrI ACTGG 1 cut(s) 429
BssECI CCNNGG 3 cut(s) 161, 362, 473
BssMI GATC 2 cut(s) 52, 452
BssNI GRCGYC 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 362
Bst2UI CCWGG 2 cut(s) 163, 475
Bst4CI ACNGT 1 cut(s) 196
BstACI GRCGYC 1 cut(s) 81
BstC8I GCNNGC 2 cut(s) 14, 61
BstDEI CTNAG 1 cut(s) 114
BstDSI CCRYGG 1 cut(s) 362
BstF5I GGATG 1 cut(s) 325
BstHHI GCGC 1 cut(s) 98
BstKTI GATC 2 cut(s) 55, 455
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 2 cut(s) 52, 452
BstMWI GCNNNNNNNGC 3 cut(s) 75, 144, 382
BstNI CCWGG 2 cut(s) 163, 475
BstSCI CCNGG 2 cut(s) 161, 473
BstSFI CTRYAG 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 41
BstV2I GAAGAC 3 cut(s) 273, 288, 384
BtgI CCRYGG 1 cut(s) 362
BtsCI GGATG 1 cut(s) 325
BtsIMutI CAGTG 1 cut(s) 436
Cac8I GCNNGC 2 cut(s) 14, 61
CfoI GCGC 1 cut(s) 98
Csp6I GTAC 1 cut(s) 40
CviAII CATG 3 cut(s) 140, 363, 439
CviJI RGCY 7 cut(s) 16, 59, 63, 113, 171, 184, 376
CviKI_1 RGCY 7 cut(s) 16, 59, 63, 113, 171, 184, 376
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 1 cut(s) 114
DpnI GATC 2 cut(s) 54, 454
DpnII GATC 2 cut(s) 52, 452
DrdI GACNNNNNNGTC 1 cut(s) 80
DseDI GACNNNNNNGTC 1 cut(s) 80
EciI GGCGGA 1 cut(s) 372
Eco130I CCWWGG 1 cut(s) 362
Eco31I GGTCTC 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 161, 473
EcoT14I CCWWGG 1 cut(s) 362
ErhI CCWWGG 1 cut(s) 362
FaeI CATG 3 cut(s) 143, 366, 442
FaiI YATR 3 cut(s) 141, 364, 440
FaqI GGGAC 2 cut(s) 86, 428
FatI CATG 3 cut(s) 139, 362, 438
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 1 cut(s) 332
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 1 cut(s) 60
GlaI GCGC 1 cut(s) 97
GluI GCNGC 1 cut(s) 30
GsaI CCCAGC 1 cut(s) 73
HapII CCGG 1 cut(s) 174
HhaI GCGC 1 cut(s) 98
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 3 cut(s) 143, 366, 442
Hin6I GCGC 1 cut(s) 96
HinP1I GCGC 1 cut(s) 96
HinfI GANTC 2 cut(s) 23, 47
HpaII CCGG 1 cut(s) 174
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 2 cut(s) 52, 156
Hpy188III TCNNGA 3 cut(s) 123, 245, 450
Hpy8I GTNNAC 1 cut(s) 91
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 2 cut(s) 224, 459
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 1 cut(s) 29
HpyF10VI GCNNNNNNNGC 3 cut(s) 75, 144, 382
HpyF3I CTNAG 1 cut(s) 114
HpySE526I ACGT 1 cut(s) 81
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 3 cut(s) 143, 366, 442
HspAI GCGC 1 cut(s) 96
Kzo9I GATC 2 cut(s) 52, 452
LmnI GCTCC 2 cut(s) 176, 313
Lsp1109I GCAGC 1 cut(s) 41
MaeI CTAG 1 cut(s) 60
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 1 cut(s) 20
MalI GATC 2 cut(s) 54, 454
MboI GATC 2 cut(s) 52, 452
MluCI AATT 1 cut(s) 211
MlyI GAGTC 1 cut(s) 17
MmeI TCCRAC 2 cut(s) 179, 215
MnlI CCTC 9 cut(s) 74, 268, 280, 286, 292, 298, 304, 387, 390
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 2 cut(s) 163, 475
Mva1269I GAATGC 1 cut(s) 118
MvaI CCWGG 2 cut(s) 163, 475
MwoI GCNNNNNNNGC 3 cut(s) 75, 144, 382
NcoI CCATGG 1 cut(s) 362
NdeII GATC 2 cut(s) 52, 452
NheI GCTAGC 1 cut(s) 59
NlaIII CATG 3 cut(s) 143, 366, 442
NlaIV GGNNCC 2 cut(s) 172, 471
NmuCI GTSAC 1 cut(s) 20
PctI GAATGC 1 cut(s) 118
PfeI GAWTC 1 cut(s) 47
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 1 cut(s) 17
PpsI GAGTC 1 cut(s) 17
Psp6I CCWGG 2 cut(s) 161, 473
PspFI CCCAGC 1 cut(s) 69
PspGI CCWGG 2 cut(s) 161, 473
PspN4I GGNNCC 2 cut(s) 172, 471
PstI CTGCAG 1 cut(s) 31
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 2 cut(s) 52, 452
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 2 cut(s) 163, 475
SetI ASST 6 cut(s) 18, 61, 84, 115, 186, 479
SfcI CTRYAG 1 cut(s) 27
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
Sse9I AATT 1 cut(s) 211
SsiI CCGC 1 cut(s) 383
SspMI CTAG 1 cut(s) 60
StyD4I CCNGG 2 cut(s) 161, 473
StyI CCWWGG 1 cut(s) 362
TaaI ACNGT 1 cut(s) 196
TaiI ACGT 1 cut(s) 84
TasI AATT 1 cut(s) 211
TatI WGTACW 1 cut(s) 39
TfiI GAWTC 1 cut(s) 47
TscAI CASTG 1 cut(s) 436
TseFI GTSAC 1 cut(s) 20
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 20
TspDTI ATGAA 2 cut(s) 17, 243
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 1 cut(s) 436
XspI CTAG 1 cut(s) 60
ZraI GACGTC 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.