Rw5G022790

B-Box-type zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001915
Physical Location & Seq
Reverse (-)
31759248 .. 31760560
1313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G022790.1

Sequence Viewer

Length: 732 bp
ATGAAAGAGTGCGAGCTGTGTGGAGTGAGAGCTCGGATGTACTGCGAGGCGGACCGCGCGAGTTTATGCTGGGACTGCGACAAGAAAGTTCACGGAGCTAACTTTCTGGTGGCGAAGCATCCGAGAGTTCTTCTCTGCCATGTGTGCCAGTCGCAGACGCCGTGGACGAGTTGCGGAGCCAAGCTGACGCCGACGGTGTCGGTCTGCCAGAGTTGCGTTGGAAGCTGTAGAAAGATTGACCTGAACGAGGAGCAAGAAAGCGAGGCAGAGAATGAAGAAGAAGAAGAAGAAGAAGATTATTATGATGATGAAGATGAAGAAGATGATGACGTTGAGGATGAGGAGGAAGAGGATGAGAACCAGGTGGTTCCATGGTCATGCTGTTCTTACTCACCGGCACAGCGTCCACCAGCAGTGAGCGGTTCGGATAGTGAAGCCGAGATTTCGGTGTCGAAGCGACTGCGAGAGAATGTAGATGTGGATTATTCTGATGATGAGGTAGGGTTTGATGGGACGTCGTCGTTTGGTGGTTCAATGAGACCGTTGAAAAGGCCGAAAGTGGGTGAAGAGAAGAATGGTGACGGAGATGATGGCGGTGAATCGGAGTGGAGAGCAACGGCGATCATGAGCTCAATTCAGAGGCTGGAAAAGGAAGTGATCAACGGCGCTGGTAATGCGTCCACCGCGGTTCTTAGGATTTGCAAACTCAGTAGAGATGAGAGCAGCCATTGA

Protein Analysis

243

Amino Acids

26.98

Weight (kDa)

4.33

Isoelectric Point (pI)

59.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 2 - 43 2.7e-06 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 518
AccBSI CCGCTC 1 cut(s) 420
AccII CGCG 3 cut(s) 57, 59, 686
AciI CCGC 7 cut(s) 50, 55, 174, 420, 594, 684, 686
AcyI GRCGYC 3 cut(s) 158, 188, 515
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 2 cut(s) 247, 560
AgsI TTSAA 2 cut(s) 534, 547
AjnI CCWGG 1 cut(s) 360
AluBI AGCT 6 cut(s) 16, 32, 98, 184, 225, 630
AluI AGCT 6 cut(s) 16, 32, 98, 184, 225, 630
Alw21I GWGCWC 2 cut(s) 34, 632
Alw26I GTCTC 1 cut(s) 532
AlwNI CAGNNNCTG 1 cut(s) 643
AoxI GGCC 1 cut(s) 551
ApeKI GCWGC 1 cut(s) 723
AspLEI GCGC 2 cut(s) 59, 668
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 4 cut(s) 384, 575, 590, 608
AvaII GGWCC 1 cut(s) 52
BanII GRGCYC 2 cut(s) 34, 632
Bbv12I GWGCWC 2 cut(s) 34, 632
BccI CCATC 2 cut(s) 503, 584
BceAI ACGGC 3 cut(s) 145, 633, 679
BcgI CGANNNNNNTGC 2 cut(s) 442, 476
BciT130I CCWGG 1 cut(s) 362
BclI TGATCA 1 cut(s) 657
BcoDI GTCTC 1 cut(s) 532
BfmI CTRYAG 1 cut(s) 226
BfoI RGCGCY 1 cut(s) 669
BisI GCNGC 1 cut(s) 724
BlsI GCNGC 1 cut(s) 725
Bme1390I CCNGG 1 cut(s) 362
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 2 cut(s) 178, 369
BmrFI CCNGG 1 cut(s) 362
BmsI GCATC 1 cut(s) 127
BplI GAGNNNNNCTC 2 cut(s) 117, 149
BsaHI GRCGYC 3 cut(s) 158, 188, 515
BsaI GGTCTC 1 cut(s) 532
BsaJI CCNNGG 3 cut(s) 161, 371, 684
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 560
Bse118I RCCGGY 1 cut(s) 394
Bse1I ACTGG 1 cut(s) 148
BseBI CCWGG 1 cut(s) 362
BseDI CCNNGG 3 cut(s) 161, 371, 684
BseGI GGATG 4 cut(s) 42, 118, 343, 358
BseLI CCNNNNNNNGG 2 cut(s) 247, 560
BseMII CTCAG 1 cut(s) 721
BseNI ACTGG 1 cut(s) 148
BseRI GAGGAG 2 cut(s) 263, 356
BseYI CCCAGC 1 cut(s) 69
Bsh1236I CGCG 3 cut(s) 57, 59, 686
BshFI GGCC 1 cut(s) 553
BsiHKAI GWGCWC 2 cut(s) 34, 632
BsiSI CCGG 1 cut(s) 395
BslFI GGGAC 2 cut(s) 86, 526
BslI CCNNNNNNNGG 2 cut(s) 247, 560
BsmAI GTCTC 1 cut(s) 532
BsmFI GGGAC 2 cut(s) 86, 526
BsnI GGCC 1 cut(s) 553
Bso31I GGTCTC 1 cut(s) 532
Bsp1286I GDGCHC 2 cut(s) 34, 632
Bsp143I GATC 2 cut(s) 621, 657
Bsp19I CCATGG 1 cut(s) 371
BspACI CCGC 7 cut(s) 50, 55, 174, 420, 594, 684, 686
BspANI GGCC 1 cut(s) 553
BspCNI CTCAG 1 cut(s) 720
BspFNI CGCG 3 cut(s) 57, 59, 686
BspHI TCATGA 1 cut(s) 624
BspLI GGNNCC 2 cut(s) 178, 369
BspTNI GGTCTC 1 cut(s) 532
BsrBI CCGCTC 1 cut(s) 420
BsrFI RCCGGY 1 cut(s) 394
BsrI ACTGG 1 cut(s) 148
BssAI RCCGGY 1 cut(s) 394
BssECI CCNNGG 3 cut(s) 161, 371, 684
BssMI GATC 2 cut(s) 621, 657
BssNI GRCGYC 3 cut(s) 158, 188, 515
BssT1I CCWWGG 1 cut(s) 371
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 196, 543
Bst6I CTCTTC 2 cut(s) 342, 561
BstACI GRCGYC 3 cut(s) 158, 188, 515
BstC8I GCNNGC 1 cut(s) 14
BstDEI CTNAG 2 cut(s) 692, 707
BstDSI CCRYGG 3 cut(s) 161, 371, 684
BstENI CCTNNNNNAGG 1 cut(s) 245
BstF5I GGATG 4 cut(s) 42, 118, 343, 358
BstFNI CGCG 3 cut(s) 57, 59, 686
BstH2I RGCGCY 1 cut(s) 669
BstHHI GCGC 2 cut(s) 59, 668
BstKTI GATC 2 cut(s) 624, 660
BstMAI GTCTC 1 cut(s) 532
BstMBI GATC 2 cut(s) 621, 657
BstMWI GCNNNNNNNGC 7 cut(s) 56, 75, 144, 213, 222, 674, 683
BstNI CCWGG 1 cut(s) 362
BstSCI CCNGG 1 cut(s) 360
BstSFI CTRYAG 1 cut(s) 226
BstUI CGCG 3 cut(s) 57, 59, 686
BsuRI GGCC 1 cut(s) 553
BtgI CCRYGG 3 cut(s) 161, 371, 684
BtsCI GGATG 4 cut(s) 42, 118, 343, 358
BtsI GCAGTG 1 cut(s) 420
BtsIMutI CAGTG 1 cut(s) 420
Cac8I GCNNGC 1 cut(s) 14
CaiI CAGNNNCTG 1 cut(s) 643
CciI TCATGA 1 cut(s) 624
CfoI GCGC 2 cut(s) 59, 668
Cfr10I RCCGGY 1 cut(s) 394
Cfr13I GGNCC 1 cut(s) 52
Cfr42I CCGCGG 1 cut(s) 687
CpoI CGGWCCG 1 cut(s) 52
CseI GACGC 4 cut(s) 166, 196, 392, 666
CsiI ACCWGGT 1 cut(s) 360
Csp6I GTAC 1 cut(s) 40
CspI CGGWCCG 1 cut(s) 52
CviAII CATG 4 cut(s) 140, 372, 378, 625
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 2 cut(s) 692, 707
DpnI GATC 2 cut(s) 623, 659
DpnII GATC 2 cut(s) 621, 657
Eam1104I CTCTTC 2 cut(s) 342, 561
EarI CTCTTC 2 cut(s) 342, 561
EciI GGCGGA 1 cut(s) 65
Ecl136II GAGCTC 2 cut(s) 32, 630
Eco130I CCWWGG 1 cut(s) 371
Eco24I GRGCYC 2 cut(s) 34, 632
Eco31I GGTCTC 1 cut(s) 532
Eco47I GGWCC 1 cut(s) 52
Eco53kI GAGCTC 2 cut(s) 32, 630
EcoICRI GAGCTC 2 cut(s) 32, 630
EcoNI CCTNNNNNAGG 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 360
EcoT14I CCWWGG 1 cut(s) 371
EcoT38I GRGCYC 2 cut(s) 34, 632
ErhI CCWWGG 1 cut(s) 371
FaeI CATG 4 cut(s) 143, 375, 381, 628
FaiI YATR 6 cut(s) 67, 141, 303, 373, 379, 626
FaqI GGGAC 2 cut(s) 86, 526
FatI CATG 4 cut(s) 139, 371, 377, 624
FbaI TGATCA 1 cut(s) 657
Fnu4HI GCNGC 1 cut(s) 724
FokI GGATG 4 cut(s) 49, 105, 350, 365
FriOI GRGCYC 2 cut(s) 34, 632
Fsp4HI GCNGC 1 cut(s) 724
GlaI GCGC 2 cut(s) 58, 667
GluI GCNGC 1 cut(s) 724
GsaI CCCAGC 1 cut(s) 73
HaeII RGCGCY 1 cut(s) 669
HaeIII GGCC 1 cut(s) 553
HapII CCGG 1 cut(s) 395
HgaI GACGC 4 cut(s) 166, 196, 392, 666
HhaI GCGC 2 cut(s) 59, 668
Hin1I GRCGYC 3 cut(s) 158, 188, 515
Hin1II CATG 4 cut(s) 143, 375, 381, 628
Hin6I GCGC 2 cut(s) 57, 666
HinP1I GCGC 2 cut(s) 57, 666
HinfI GANTC 1 cut(s) 599
HpaII CCGG 1 cut(s) 395
HphI GGTGA 4 cut(s) 384, 575, 590, 608
Hpy166II GTNNAC 4 cut(s) 91, 165, 407, 681
Hpy188I TCNGA 6 cut(s) 36, 123, 427, 490, 604, 639
Hpy188III TCNNGA 1 cut(s) 625
Hpy8I GTNNAC 4 cut(s) 91, 165, 407, 681
Hpy99I CGWCG 3 cut(s) 196, 520, 523
HpyCH4III ACNGT 2 cut(s) 196, 543
HpyCH4IV ACGT 2 cut(s) 330, 515
HpyCH4V TGCA 1 cut(s) 702
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 75, 144, 213, 222, 674, 683
HpyF3I CTNAG 2 cut(s) 692, 707
HpySE526I ACGT 2 cut(s) 330, 515
Hsp92I GRCGYC 3 cut(s) 158, 188, 515
Hsp92II CATG 4 cut(s) 143, 375, 381, 628
HspAI GCGC 2 cut(s) 57, 666
Ksp22I TGATCA 1 cut(s) 657
KspI CCGCGG 1 cut(s) 687
Kzo9I GATC 2 cut(s) 621, 657
LmnI GCTCC 3 cut(s) 95, 176, 250
LweI GCATC 1 cut(s) 127
MabI ACCWGGT 1 cut(s) 360
MaeII ACGT 2 cut(s) 330, 515
MaeIII GTNAC 1 cut(s) 578
MalI GATC 2 cut(s) 623, 659
MbiI CCGCTC 1 cut(s) 420
MboI GATC 2 cut(s) 621, 657
MhlI GDGCHC 2 cut(s) 34, 632
MluCI AATT 1 cut(s) 633
MmeI TCCRAC 1 cut(s) 199
MnlI CCTC 9 cut(s) 40, 241, 256, 328, 334, 337, 343, 490, 633
MslI CAYNNNNRTG 1 cut(s) 376
MspA1I CMGCKG 1 cut(s) 686
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 1 cut(s) 362
MvaI CCWGG 1 cut(s) 362
MvnI CGCG 3 cut(s) 57, 59, 686
MwoI GCNNNNNNNGC 7 cut(s) 56, 75, 144, 213, 222, 674, 683
NcoI CCATGG 1 cut(s) 371
NdeII GATC 2 cut(s) 621, 657
NlaIII CATG 4 cut(s) 143, 375, 381, 628
NlaIV GGNNCC 2 cut(s) 178, 369
NmeAIII GCCGAG 1 cut(s) 463
NmuCI GTSAC 1 cut(s) 578
PagI TCATGA 1 cut(s) 624
PcsI WCGNNNNNNNCGW 2 cut(s) 158, 164
PfeI GAWTC 1 cut(s) 599
PflFI GACNNNGTC 2 cut(s) 196, 517
PkrI GCNGC 1 cut(s) 725
Psp124BI GAGCTC 2 cut(s) 34, 632
Psp6I CCWGG 1 cut(s) 360
PspFI CCCAGC 1 cut(s) 69
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 2 cut(s) 178, 369
PspPI GGNCC 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 643
PsyI GACNNNGTC 2 cut(s) 196, 517
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
RseI CAYNNNNRTG 1 cut(s) 376
Rsr2I CGGWCCG 1 cut(s) 52
RsrII CGGWCCG 1 cut(s) 52
SacI GAGCTC 2 cut(s) 34, 632
SacII CCGCGG 1 cut(s) 687
SatI GCNGC 1 cut(s) 724
Sau3AI GATC 2 cut(s) 621, 657
Sau96I GGNCC 1 cut(s) 52
ScrFI CCNGG 1 cut(s) 362
SduI GDGCHC 2 cut(s) 34, 632
SexAI ACCWGGT 1 cut(s) 360
SfaNI GCATC 1 cut(s) 127
SfcI CTRYAG 1 cut(s) 226
Sfr303I CCGCGG 1 cut(s) 687
SgrBI CCGCGG 1 cut(s) 687
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 376
Sse9I AATT 1 cut(s) 633
SsiI CCGC 7 cut(s) 50, 55, 174, 420, 594, 684, 686
SstI GAGCTC 2 cut(s) 34, 632
StyD4I CCNGG 1 cut(s) 360
StyI CCWWGG 1 cut(s) 371
TaaI ACNGT 2 cut(s) 196, 543
TaiI ACGT 2 cut(s) 333, 518
TaqI TCGA 1 cut(s) 452
TaqII GACCGA 1 cut(s) 190
TasI AATT 1 cut(s) 633
TatI WGTACW 1 cut(s) 39
TfiI GAWTC 1 cut(s) 599
TscAI CASTG 1 cut(s) 420
TseFI GTSAC 1 cut(s) 578
TseI GCWGC 1 cut(s) 723
Tsp45I GTSAC 1 cut(s) 578
TspDTI ATGAA 4 cut(s) 17, 288, 324, 330
TspGWI ACGGA 2 cut(s) 108, 597
TspRI CASTG 1 cut(s) 420
Tth111I GACNNNGTC 2 cut(s) 196, 517
VpaK11BI GGWCC 1 cut(s) 52
XagI CCTNNNNNAGG 1 cut(s) 245
XcmI CCANNNNNNNNNTGG 1 cut(s) 215
ZraI GACGTC 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.