Rh5AG248300

B-Box-type zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001915
Physical Location & Seq
Reverse (-)
30893112 .. 30894837
1726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG248300.1

Sequence Viewer

Length: 726 bp
ATGAAAGAGTGCGAGCTGTGTGGAGTGAGAGCTCGGATGTACTGCGAGGCGGACCGCGCGAGTTTATGCTGGGACTGCGACGAGAAAGTTCACGGAGCTAACTTTCTGGTGGCGAAGCATCCGAGAGTTCTTCTCTGCCATGTGTGCCAGTCGCAGACGCCGTGGACGAGTTGCGGAGCCAAGCTGACGCCGACGGTGTCGGTCTGCCAGAGTTGCGTTGGAAGCTGTAGTAAGATTGACCTGAACCAGGAGCAAGAAAGCGAGGCAGAGAATGAAGAAGAAGAAGAAGATTATTATGATGATGAAGATGAAGAAGATGATGACGTTGAGGATGAGGAGGAAGAGGATGAGAACCAGGTGGTTCCATGGTCATGCTGTTCTTACTCACCGGCACAGCGTCCACCAGCAGTGAGCGGTTCGGATAGTGAAGCCGAGATTTCGGTGTCGAAGCGACTGCGAGAGAATGTAGATGTGGATTATTCTGATGATGAGGTAGGGTTTGATGGGACGTCGTCGTTTGGTGGTTCAATGAGACCGTTGAAAAGGCCGAAAGTGGGTGAAGAGAAGAATGGTGACGGAGATGATGGCGGTGAATCGGAGTGGAGAGCAACGGCGATCATGAGCTCAATTCAGAGGCTGGAAAAGGAAGTGATCAACGGCGCTGGTAATGCGTCCACCGCGGTTCTTAGGATTTGCAAACTCAGTAGAGATGAGAGCAGCCATTGA

Protein Analysis

241

Amino Acids

26.66

Weight (kDa)

4.29

Isoelectric Point (pI)

56.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 2 - 43 2.1e-06 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 512
AccBSI CCGCTC 1 cut(s) 414
AccII CGCG 3 cut(s) 57, 59, 680
AciI CCGC 7 cut(s) 50, 55, 174, 414, 588, 678, 680
AcyI GRCGYC 3 cut(s) 158, 188, 509
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 2 cut(s) 247, 554
AgsI TTSAA 2 cut(s) 528, 541
AjnI CCWGG 2 cut(s) 246, 354
AluBI AGCT 6 cut(s) 16, 32, 98, 184, 225, 624
AluI AGCT 6 cut(s) 16, 32, 98, 184, 225, 624
Alw21I GWGCWC 2 cut(s) 34, 626
Alw26I GTCTC 1 cut(s) 526
AlwNI CAGNNNCTG 1 cut(s) 637
AoxI GGCC 1 cut(s) 545
ApeKI GCWGC 1 cut(s) 717
AspLEI GCGC 2 cut(s) 59, 662
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 4 cut(s) 378, 569, 584, 602
AvaII GGWCC 1 cut(s) 52
BanII GRGCYC 2 cut(s) 34, 626
Bbv12I GWGCWC 2 cut(s) 34, 626
BccI CCATC 2 cut(s) 497, 578
BceAI ACGGC 3 cut(s) 145, 627, 673
BcgI CGANNNNNNTGC 2 cut(s) 436, 470
BciT130I CCWGG 2 cut(s) 248, 356
BclI TGATCA 1 cut(s) 651
BcoDI GTCTC 1 cut(s) 526
BfmI CTRYAG 1 cut(s) 226
BfoI RGCGCY 1 cut(s) 663
BisI GCNGC 1 cut(s) 718
BlsI GCNGC 1 cut(s) 719
Bme1390I CCNGG 2 cut(s) 248, 356
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 2 cut(s) 178, 363
BmrFI CCNGG 2 cut(s) 248, 356
BmsI GCATC 1 cut(s) 127
BplI GAGNNNNNCTC 2 cut(s) 117, 149
BsaHI GRCGYC 3 cut(s) 158, 188, 509
BsaI GGTCTC 1 cut(s) 526
BsaJI CCNNGG 3 cut(s) 161, 365, 678
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 554
Bse118I RCCGGY 1 cut(s) 388
Bse1I ACTGG 1 cut(s) 148
BseBI CCWGG 2 cut(s) 248, 356
BseDI CCNNGG 3 cut(s) 161, 365, 678
BseGI GGATG 4 cut(s) 42, 118, 337, 352
BseLI CCNNNNNNNGG 2 cut(s) 247, 554
BseMII CTCAG 1 cut(s) 715
BseNI ACTGG 1 cut(s) 148
BseRI GAGGAG 1 cut(s) 350
BseYI CCCAGC 1 cut(s) 69
Bsh1236I CGCG 3 cut(s) 57, 59, 680
BshFI GGCC 1 cut(s) 547
BsiHKAI GWGCWC 2 cut(s) 34, 626
BsiSI CCGG 1 cut(s) 389
BslFI GGGAC 2 cut(s) 86, 520
BslI CCNNNNNNNGG 2 cut(s) 247, 554
BsmAI GTCTC 1 cut(s) 526
BsmFI GGGAC 2 cut(s) 86, 520
BsnI GGCC 1 cut(s) 547
Bso31I GGTCTC 1 cut(s) 526
Bsp1286I GDGCHC 2 cut(s) 34, 626
Bsp143I GATC 2 cut(s) 615, 651
Bsp19I CCATGG 1 cut(s) 365
BspACI CCGC 7 cut(s) 50, 55, 174, 414, 588, 678, 680
BspANI GGCC 1 cut(s) 547
BspCNI CTCAG 1 cut(s) 714
BspFNI CGCG 3 cut(s) 57, 59, 680
BspHI TCATGA 1 cut(s) 618
BspLI GGNNCC 2 cut(s) 178, 363
BspTNI GGTCTC 1 cut(s) 526
BsrBI CCGCTC 1 cut(s) 414
BsrFI RCCGGY 1 cut(s) 388
BsrI ACTGG 1 cut(s) 148
BssAI RCCGGY 1 cut(s) 388
BssECI CCNNGG 3 cut(s) 161, 365, 678
BssMI GATC 2 cut(s) 615, 651
BssNI GRCGYC 3 cut(s) 158, 188, 509
BssT1I CCWWGG 1 cut(s) 365
Bst2UI CCWGG 2 cut(s) 248, 356
Bst4CI ACNGT 2 cut(s) 196, 537
Bst6I CTCTTC 2 cut(s) 336, 555
BstACI GRCGYC 3 cut(s) 158, 188, 509
BstC8I GCNNGC 1 cut(s) 14
BstDEI CTNAG 2 cut(s) 686, 701
BstDSI CCRYGG 3 cut(s) 161, 365, 678
BstENI CCTNNNNNAGG 1 cut(s) 245
BstF5I GGATG 4 cut(s) 42, 118, 337, 352
BstFNI CGCG 3 cut(s) 57, 59, 680
BstH2I RGCGCY 1 cut(s) 663
BstHHI GCGC 2 cut(s) 59, 662
BstKTI GATC 2 cut(s) 618, 654
BstMAI GTCTC 1 cut(s) 526
BstMBI GATC 2 cut(s) 615, 651
BstMWI GCNNNNNNNGC 7 cut(s) 56, 75, 144, 213, 222, 668, 677
BstNI CCWGG 2 cut(s) 248, 356
BstSCI CCNGG 2 cut(s) 246, 354
BstSFI CTRYAG 1 cut(s) 226
BstUI CGCG 3 cut(s) 57, 59, 680
BsuRI GGCC 1 cut(s) 547
BtgI CCRYGG 3 cut(s) 161, 365, 678
BtsCI GGATG 4 cut(s) 42, 118, 337, 352
BtsI GCAGTG 1 cut(s) 414
BtsIMutI CAGTG 1 cut(s) 414
Cac8I GCNNGC 1 cut(s) 14
CaiI CAGNNNCTG 1 cut(s) 637
CciI TCATGA 1 cut(s) 618
CfoI GCGC 2 cut(s) 59, 662
Cfr10I RCCGGY 1 cut(s) 388
Cfr13I GGNCC 1 cut(s) 52
Cfr42I CCGCGG 1 cut(s) 681
CpoI CGGWCCG 1 cut(s) 52
CseI GACGC 4 cut(s) 166, 196, 386, 660
CsiI ACCWGGT 1 cut(s) 354
Csp6I GTAC 1 cut(s) 40
CspI CGGWCCG 1 cut(s) 52
CviAII CATG 4 cut(s) 140, 366, 372, 619
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 2 cut(s) 686, 701
DpnI GATC 2 cut(s) 617, 653
DpnII GATC 2 cut(s) 615, 651
Eam1104I CTCTTC 2 cut(s) 336, 555
EarI CTCTTC 2 cut(s) 336, 555
EciI GGCGGA 1 cut(s) 65
Ecl136II GAGCTC 2 cut(s) 32, 624
Eco130I CCWWGG 1 cut(s) 365
Eco24I GRGCYC 2 cut(s) 34, 626
Eco31I GGTCTC 1 cut(s) 526
Eco47I GGWCC 1 cut(s) 52
Eco53kI GAGCTC 2 cut(s) 32, 624
EcoICRI GAGCTC 2 cut(s) 32, 624
EcoNI CCTNNNNNAGG 1 cut(s) 245
EcoRII CCWGG 2 cut(s) 246, 354
EcoT14I CCWWGG 1 cut(s) 365
EcoT38I GRGCYC 2 cut(s) 34, 626
ErhI CCWWGG 1 cut(s) 365
FaeI CATG 4 cut(s) 143, 369, 375, 622
FaiI YATR 6 cut(s) 67, 141, 297, 367, 373, 620
FaqI GGGAC 2 cut(s) 86, 520
FatI CATG 4 cut(s) 139, 365, 371, 618
FbaI TGATCA 1 cut(s) 651
Fnu4HI GCNGC 1 cut(s) 718
FokI GGATG 4 cut(s) 49, 105, 344, 359
FriOI GRGCYC 2 cut(s) 34, 626
Fsp4HI GCNGC 1 cut(s) 718
GlaI GCGC 2 cut(s) 58, 661
GluI GCNGC 1 cut(s) 718
GsaI CCCAGC 1 cut(s) 73
HaeII RGCGCY 1 cut(s) 663
HaeIII GGCC 1 cut(s) 547
HapII CCGG 1 cut(s) 389
HgaI GACGC 4 cut(s) 166, 196, 386, 660
HhaI GCGC 2 cut(s) 59, 662
Hin1I GRCGYC 3 cut(s) 158, 188, 509
Hin1II CATG 4 cut(s) 143, 369, 375, 622
Hin6I GCGC 2 cut(s) 57, 660
HinP1I GCGC 2 cut(s) 57, 660
HinfI GANTC 1 cut(s) 593
HpaII CCGG 1 cut(s) 389
HphI GGTGA 4 cut(s) 378, 569, 584, 602
Hpy166II GTNNAC 4 cut(s) 91, 165, 401, 675
Hpy188I TCNGA 6 cut(s) 36, 123, 421, 484, 598, 633
Hpy188III TCNNGA 1 cut(s) 619
Hpy8I GTNNAC 4 cut(s) 91, 165, 401, 675
Hpy99I CGWCG 4 cut(s) 83, 196, 514, 517
HpyCH4III ACNGT 2 cut(s) 196, 537
HpyCH4IV ACGT 2 cut(s) 324, 509
HpyCH4V TGCA 1 cut(s) 696
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 75, 144, 213, 222, 668, 677
HpyF3I CTNAG 2 cut(s) 686, 701
HpySE526I ACGT 2 cut(s) 324, 509
Hsp92I GRCGYC 3 cut(s) 158, 188, 509
Hsp92II CATG 4 cut(s) 143, 369, 375, 622
HspAI GCGC 2 cut(s) 57, 660
Ksp22I TGATCA 1 cut(s) 651
KspI CCGCGG 1 cut(s) 681
Kzo9I GATC 2 cut(s) 615, 651
LmnI GCTCC 3 cut(s) 95, 176, 250
LweI GCATC 1 cut(s) 127
MabI ACCWGGT 1 cut(s) 354
MaeII ACGT 2 cut(s) 324, 509
MaeIII GTNAC 1 cut(s) 572
MalI GATC 2 cut(s) 617, 653
MbiI CCGCTC 1 cut(s) 414
MboI GATC 2 cut(s) 615, 651
MhlI GDGCHC 2 cut(s) 34, 626
MluCI AATT 1 cut(s) 627
MmeI TCCRAC 1 cut(s) 199
MnlI CCTC 8 cut(s) 40, 256, 322, 328, 331, 337, 484, 627
MslI CAYNNNNRTG 1 cut(s) 370
MspA1I CMGCKG 1 cut(s) 680
MspI CCGG 1 cut(s) 389
MspR9I CCNGG 2 cut(s) 248, 356
MvaI CCWGG 2 cut(s) 248, 356
MvnI CGCG 3 cut(s) 57, 59, 680
MwoI GCNNNNNNNGC 7 cut(s) 56, 75, 144, 213, 222, 668, 677
NcoI CCATGG 1 cut(s) 365
NdeII GATC 2 cut(s) 615, 651
NlaIII CATG 4 cut(s) 143, 369, 375, 622
NlaIV GGNNCC 2 cut(s) 178, 363
NmeAIII GCCGAG 1 cut(s) 457
NmuCI GTSAC 1 cut(s) 572
PagI TCATGA 1 cut(s) 618
PcsI WCGNNNNNNNCGW 2 cut(s) 158, 164
PfeI GAWTC 1 cut(s) 593
PflFI GACNNNGTC 2 cut(s) 196, 511
PkrI GCNGC 1 cut(s) 719
Psp124BI GAGCTC 2 cut(s) 34, 626
Psp6I CCWGG 2 cut(s) 246, 354
PspFI CCCAGC 1 cut(s) 69
PspGI CCWGG 2 cut(s) 246, 354
PspN4I GGNNCC 2 cut(s) 178, 363
PspPI GGNCC 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 637
PsyI GACNNNGTC 2 cut(s) 196, 511
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
RseI CAYNNNNRTG 1 cut(s) 370
Rsr2I CGGWCCG 1 cut(s) 52
RsrII CGGWCCG 1 cut(s) 52
SacI GAGCTC 2 cut(s) 34, 626
SacII CCGCGG 1 cut(s) 681
SatI GCNGC 1 cut(s) 718
Sau3AI GATC 2 cut(s) 615, 651
Sau96I GGNCC 1 cut(s) 52
ScrFI CCNGG 2 cut(s) 248, 356
SduI GDGCHC 2 cut(s) 34, 626
SexAI ACCWGGT 1 cut(s) 354
SfaNI GCATC 1 cut(s) 127
SfcI CTRYAG 1 cut(s) 226
Sfr303I CCGCGG 1 cut(s) 681
SgrBI CCGCGG 1 cut(s) 681
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 370
Sse9I AATT 1 cut(s) 627
SsiI CCGC 7 cut(s) 50, 55, 174, 414, 588, 678, 680
SstI GAGCTC 2 cut(s) 34, 626
StyD4I CCNGG 2 cut(s) 246, 354
StyI CCWWGG 1 cut(s) 365
TaaI ACNGT 2 cut(s) 196, 537
TaiI ACGT 2 cut(s) 327, 512
TaqI TCGA 1 cut(s) 446
TaqII GACCGA 1 cut(s) 190
TasI AATT 1 cut(s) 627
TatI WGTACW 1 cut(s) 39
TfiI GAWTC 1 cut(s) 593
TscAI CASTG 1 cut(s) 414
TseFI GTSAC 1 cut(s) 572
TseI GCWGC 1 cut(s) 717
Tsp45I GTSAC 1 cut(s) 572
TspDTI ATGAA 4 cut(s) 17, 288, 318, 324
TspGWI ACGGA 2 cut(s) 108, 591
TspRI CASTG 1 cut(s) 414
Tth111I GACNNNGTC 2 cut(s) 196, 511
VpaK11BI GGWCC 1 cut(s) 52
XagI CCTNNNNNAGG 1 cut(s) 245
XcmI CCANNNNNNNNNTGG 1 cut(s) 215
ZraI GACGTC 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.