MD09G1189300.v1.1

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000818
Physical Location & Seq
Forward (+)
16518368 .. 16518685
318 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1189300.v1.1.491

Sequence Viewer

Length: 318 bp
ATGAACCAGAAAACTCGCGATACTGTTATGCGCGAATTTCGCAGATGCTCTTGCCGCATCCTCATTACCACCGATCAATATGCTCGTCATCTTGAGGTCCAGCAAGCGTTTGTTGTAGTAGATTATGATTTGCCGGCCGATCAACCAGAAAACTACCTTCATCGTATAGGACGAAGCGGAAATTTTGGAAAAAGAGGTGCTGCCATCAACTTCGTGGCTAGAGATGGGGAGAAAATGCTTTTTGACATTCAGAGGAGCTGCCAGCAAATTTTGATGATTTCCTCTGAGCATTTGGTATTCAATTTCCTACCCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

106

Amino Acids

12.3

Weight (kDa)

7.76

Isoelectric Point (pI)

49.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 1 - 63 2.9e-13 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 18, 33
AciI CCGC 2 cut(s) 55, 177
AcoI YGGCCR 1 cut(s) 135
AcsI RAATTY 3 cut(s) 35, 181, 267
AgsI TTSAA 1 cut(s) 301
AluBI AGCT 1 cut(s) 258
AluI AGCT 1 cut(s) 258
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 1 cut(s) 135
ApeKI GCWGC 2 cut(s) 200, 258
ApoI RAATTY 3 cut(s) 35, 181, 267
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 1 cut(s) 97
AvaI CYCGRG 1 cut(s) 311
AvaII GGWCC 1 cut(s) 97
BbvI GCAGC 2 cut(s) 187, 245
BccI CCATC 2 cut(s) 212, 218
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BfaI CTAG 1 cut(s) 219
BisI GCNGC 3 cut(s) 55, 201, 259
BlsI GCNGC 3 cut(s) 56, 202, 260
Bme18I GGWCC 1 cut(s) 97
BmeT110I CYCGRG 1 cut(s) 311
BmgT120I GGNCC 1 cut(s) 97
BmsI GCATC 2 cut(s) 35, 66
BpuEI CTTGAG 1 cut(s) 113
Bse118I RCCGGY 1 cut(s) 133
BseGI GGATG 1 cut(s) 57
BseMII CTCAG 1 cut(s) 276
BseRI GAGGAG 1 cut(s) 268
BseX3I CGGCCG 1 cut(s) 135
BseXI GCAGC 2 cut(s) 187, 245
Bsh1236I CGCG 2 cut(s) 18, 33
Bsh1285I CGRYCG 1 cut(s) 138
BshFI GGCC 1 cut(s) 137
BsiEI CGRYCG 1 cut(s) 138
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 1 cut(s) 134
BsnI GGCC 1 cut(s) 137
BsoBI CYCGRG 1 cut(s) 311
Bsp143I GATC 2 cut(s) 73, 139
Bsp68I TCGCGA 1 cut(s) 18
BspACI CCGC 2 cut(s) 55, 177
BspANI GGCC 1 cut(s) 137
BspCNI CTCAG 1 cut(s) 277
BspFNI CGCG 2 cut(s) 18, 33
BsrFI RCCGGY 1 cut(s) 133
BssAI RCCGGY 1 cut(s) 133
BssMI GATC 2 cut(s) 73, 139
Bst4CI ACNGT 1 cut(s) 25
BstC8I GCNNGC 3 cut(s) 105, 135, 263
BstDEI CTNAG 1 cut(s) 285
BstF5I GGATG 1 cut(s) 57
BstFNI CGCG 2 cut(s) 18, 33
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 2 cut(s) 76, 142
BstMBI GATC 2 cut(s) 73, 139
BstMCI CGRYCG 1 cut(s) 138
BstMWI GCNNNNNNNGC 2 cut(s) 39, 54
BstUI CGCG 2 cut(s) 18, 33
BstV1I GCAGC 2 cut(s) 187, 245
BstZI CGGCCG 1 cut(s) 135
BsuRI GGCC 1 cut(s) 137
BtsCI GGATG 1 cut(s) 57
BtuMI TCGCGA 1 cut(s) 18
Cac8I GCNNGC 3 cut(s) 105, 135, 263
CfoI GCGC 1 cut(s) 33
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 1 cut(s) 97
CviJI RGCY 3 cut(s) 137, 218, 258
CviKI_1 RGCY 3 cut(s) 137, 218, 258
DdeI CTNAG 1 cut(s) 285
DpnI GATC 2 cut(s) 75, 141
DpnII GATC 2 cut(s) 73, 139
EaeI YGGCCR 1 cut(s) 135
EagI CGGCCG 1 cut(s) 135
EclXI CGGCCG 1 cut(s) 135
Eco47I GGWCC 1 cut(s) 97
Eco52I CGGCCG 1 cut(s) 135
Eco88I CYCGRG 1 cut(s) 311
FaiI YATR 4 cut(s) 29, 81, 126, 167
Fnu4HI GCNGC 3 cut(s) 55, 201, 259
FokI GGATG 1 cut(s) 44
Fsp4HI GCNGC 3 cut(s) 55, 201, 259
FspBI CTAG 1 cut(s) 219
GlaI GCGC 1 cut(s) 32
GluI GCNGC 3 cut(s) 55, 201, 259
HaeIII GGCC 1 cut(s) 137
HapII CCGG 1 cut(s) 134
HhaI GCGC 1 cut(s) 33
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HpaII CCGG 1 cut(s) 134
Hpy188I TCNGA 2 cut(s) 252, 286
Hpy188III TCNNGA 2 cut(s) 17, 92
HpyAV CCTTC 1 cut(s) 167
HpyCH4III ACNGT 1 cut(s) 25
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 54
HpyF3I CTNAG 1 cut(s) 285
HspAI GCGC 1 cut(s) 31
KroI GCCGGC 1 cut(s) 133
KroNI GCCGGC 1 cut(s) 135
Kzo9I GATC 2 cut(s) 73, 139
LmnI GCTCC 1 cut(s) 255
LpnPI CCDG 5 cut(s) 20, 113, 147, 159, 275
Lsp1109I GCAGC 2 cut(s) 187, 245
LweI GCATC 2 cut(s) 35, 66
MaeI CTAG 1 cut(s) 219
MalI GATC 2 cut(s) 75, 141
MboI GATC 2 cut(s) 73, 139
MluCI AATT 4 cut(s) 35, 181, 267, 301
MnlI CCTC 5 cut(s) 71, 88, 188, 246, 292
MroNI GCCGGC 1 cut(s) 133
MspI CCGG 1 cut(s) 134
MvnI CGCG 2 cut(s) 18, 33
MwoI GCNNNNNNNGC 2 cut(s) 39, 54
NaeI GCCGGC 1 cut(s) 135
NdeII GATC 2 cut(s) 73, 139
NgoMIV GCCGGC 1 cut(s) 133
NruI TCGCGA 1 cut(s) 18
PcsI WCGNNNNNNNCGW 1 cut(s) 169
PdiI GCCGGC 1 cut(s) 135
PkrI GCNGC 3 cut(s) 56, 202, 260
PspPI GGNCC 1 cut(s) 97
RruI TCGCGA 1 cut(s) 18
SatI GCNGC 3 cut(s) 55, 201, 259
Sau3AI GATC 2 cut(s) 73, 139
Sau96I GGNCC 1 cut(s) 97
SetI ASST 4 cut(s) 99, 159, 199, 260
SfaNI GCATC 2 cut(s) 35, 66
SinI GGWCC 1 cut(s) 97
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 4 cut(s) 35, 181, 267, 301
SsiI CCGC 2 cut(s) 55, 177
SspMI CTAG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 25
TasI AATT 4 cut(s) 35, 181, 267, 301
TauI GCSGC 1 cut(s) 57
TseI GCWGC 2 cut(s) 200, 258
TspDTI ATGAA 2 cut(s) 17, 149
VpaK11BI GGWCC 1 cut(s) 97
XapI RAATTY 3 cut(s) 35, 181, 267
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
XspI CTAG 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.