Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000818

Physical Location & Seq

Genomic Coordinates Forward (+)

34294490 .. 34295993

Gene Structure 1504 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1268600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1167 bp

ATGGCGCCATTCGCGACTTCTGATGGGATAGAGGCGATCATGAGCTTCGATGAGATGGGGTTGAAGGATGATGTGCTCCAAGGCATCTACGAATATGGGTACGAAAAGCCCTCCGCCATCCAGCAACGGGCGGTCAGGCGCATTATCGAGGGTTATGATGTCATAGCCCAGTCCCAGTCAGGTACCGGCAAAACCTCCATGATTGCCCTTACCGCTTGCCAACTCGTCGACACCTCCTGCCGAGAGGTTCAGGCTTTGATATTGTGTCCCACTAGGGAACTGGCGGCACAGACGGAGAAGGTGATATTAGCAATTGGAAATCACATGAACATACAAGCGCATGCTTGCATCGGAGGCAAAAGCGTGGCTGAGGATATTCGAAAGCTTGAGCACGGAGTTCATGTGGTGTGTGGAACTCCAGGTAGAGTCCATGACATGATCAAGAGGAGAAGTCTGCGTACTCGAGCCATTAAATTACTATTCCTCGACGAATCTGATGAAATGTTAAGCAGATCATTCAAGGATCAGATTTACAATGTCTATAGATATCTTCCACCTGAACTACAAGTCTGCTTAATTTCCGCTACGCTTCCTCATGAAATCCTGGAGATGACGAGCAAGTTTATGACGGACCCTGTAAGGATCCTTGTCAAGCGTGATGAGTTGACTTTGGAGGGAATCAAGCAATTTTTCGTTTCGGTTGAAAGAGAAGAATGGAAATTTGATACGCTGTGCGATCTCTATGACACTCTTACAATTACACAAGCTGTTATTTTCTGCAACACAAAGCGAAAGGTGGATTGGCTGACAGAAAAGATGCGGAGGAGTAATAACTTTACTGTATCTTCCATGCATGCAGACATGCCTCAAAAGGAAAGAGATGTCATTACCCAAGAGTTTCGTGCAGGAAATACTCGTGTCCTGATCACCACTGATGTTTGGGCTCGGGGCCTCGATGTTCAGCAGGTTTCCTTGGTTATCAACTATGATCTTCCAAACAATCGAGAGTTATACATTCATCGGATTGGTCGTTCTGGTCGTTTTGGGCGCAAGGGTGTGGCCATAAACTTTGTCAGAACCAATGATATCAAGATCTTAAGAGACATCGAGCAGTACTACAGTACCCAGATTGACGAAATGCCGATGAACGTTGGTGATTTGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009628 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0016020 GO:0016070 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035145 GO:0036293 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050896 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070482 GO:0071013 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03013 ko03015 ko03040 map03013 map03015 map03040

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

389

Amino Acids

44.34

Weight (kDa)

6.28

Isoelectric Point (pI)

45.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	37 - 189	1.4e-06	Type III restriction enzyme, res subunit
DEAD	PF00270	39 - 201	4.7e-41	DEAD/DEAH box helicase
Helicase_C	PF00271	240 - 349	2.8e-34	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000818)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51380 AT1G51380 AT1G51380 AT1G51380 AT3G19760
fragaria_vesca	FvH4_4g36800 FvH4_4g36800 FvH4_4g36800 FvH4_6g49811 FvH4_6g49900
malus_domestica	MD09G1053900.v1.1 MD09G1189300.v1.1 MD09G1268200.v1.1 MD09G1268600.v1.1 MD09G1268800.v1.1 MD13G1006800.v1.1 MD15G1056500.v1.1 MD16G1001000.v1.1
prunus_persica	Prupe.1G346100_v2.0.a1 Prupe.3G165200_v2.0.a1
pyrus_communis	pycom111g04510 pycom16g00440
rosa_chinensis	RchiOBHm_Chr0c44g0503461 RchiOBHm_Chr0c44g0503501 RchiOBHm_Chr0c44g0503531 RchiOBHm_Chr0c44g0503561 RchiOBHm_Chr0c44g0503691 RchiOBHm_Chr0c44g0503701 RchiOBHm_Chr0c44g0503721 RchiOBHm_Chr0c44g0503751 RchiOBHm_Chr2g0169961 RchiOBHm_Chr4g0386061 RchiOBHm_Chr4g0386101 RchiOBHm_Chr4g0386151 RchiOBHm_Chr4g0386181 RchiOBHm_Chr4g0386221 RchiOBHm_Chr4g0386241 RchiOBHm_Chr4g0386301 RchiOBHm_Chr4g0386341 RchiOBHm_Chr4g0386361 RchiOBHm_Chr4g0446531
rosa_laevigata	RLG00000005651 RLG00000010170 RLG00000010175 RLG00000021914
rosa_multiflora	Rmu_sc0005526.1_g000001 Rmu_sc0005795.1_g000003 Rmu_sc0013078.1_g000019 Rmu_sc0022031.1_g000001 Rmu_ssc0000389.1_g000007
rosa_roxburghii	Rroxscaffold_5G00333840 Rroxscaffold_5G00386950
rosa_rugosa	Rorug02G0544700 Rorug02G0545100 Rorug04G0372300
rosa_samantha	Rh2AG617000 Rh2AG617300 Rh2CG597900 Rh2DG639700 Rh2DG640100 Rh2DG640400 Rh4AG010700 Rh4AG433900 Rh4BG007600 Rh4BG443100 Rh4DG441700
rosa_wichuraiana	Rw2G051090 Rw4G037080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	955
Acc65I	GGTACC	1 cut(s)	182
AccB1I	GGYRCC	2 cut(s)	4, 182
AccI	GTMKAC	1 cut(s)	228
AccII	CGCG	1 cut(s)	14
AciI	CCGC	6 cut(s)	114, 131, 213, 284, 582, 820
AclI	AACGTT	1 cut(s)	1149
AclWI	GGATC	3 cut(s)	531, 637, 650
AcoI	YGGCCR	1 cut(s)	1059
AcsI	RAATTY	1 cut(s)	719
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	5 cut(s)	101, 184, 460, 1115, 1123
AfiI	CCNNNNNNNGG	1 cut(s)	127
AflII	CTTAAG	1 cut(s)	1096
AgsI	TTSAA	3 cut(s)	64, 520, 704
AjnI	CCWGG	2 cut(s)	418, 603
AjuI	GAANNNNNNNTTGG	2 cut(s)	784, 816
AloI	GAACNNNNNNTCC	2 cut(s)	1015, 1047
AluBI	AGCT	3 cut(s)	45, 385, 767
AluI	AGCT	3 cut(s)	45, 385, 767
Alw21I	GWGCWC	2 cut(s)	78, 393
Alw26I	GTCTC	1 cut(s)	1095
AlwI	GGATC	3 cut(s)	531, 637, 650
Ama87I	CYCGRG	2 cut(s)	462, 945
AoxI	GGCC	2 cut(s)	949, 1059
ApoI	RAATTY	1 cut(s)	719
Asp718I	GGTACC	1 cut(s)	182
AspLEI	GCGC	4 cut(s)	7, 141, 340, 1050
AspS9I	GGNCC	2 cut(s)	631, 949
AsuHPI	GGTGA	3 cut(s)	313, 919, 1166
AsuII	TTCGAA	1 cut(s)	379
AvaI	CYCGRG	2 cut(s)	462, 945
AvaII	GGWCC	1 cut(s)	631
BaeI	ACNNNNGTAYC	2 cut(s)	1105, 1138
BalI	TGGCCA	1 cut(s)	1061
BamHI	GGATCC	1 cut(s)	642
BanI	GGYRCC	2 cut(s)	4, 182
BanII	GRGCYC	1 cut(s)	946
BarI	GAAGNNNNNNTAC	2 cut(s)	442, 474
BauI	CACGAG	1 cut(s)	915
Bbv12I	GWGCWC	2 cut(s)	78, 393
BbvCI	CCTCAGC	1 cut(s)	369
BccI	CCATC	3 cut(s)	17, 49, 125
BciT130I	CCWGG	2 cut(s)	420, 605
BclI	TGATCA	2 cut(s)	438, 924
BcoDI	GTCTC	1 cut(s)	1095
BfaI	CTAG	1 cut(s)	273
BfmI	CTRYAG	2 cut(s)	541, 1117
BfoI	RGCGCY	1 cut(s)	8
BfrI	CTTAAG	1 cut(s)	1096
BfuAI	ACCTGC	1 cut(s)	955
BglII	AGATCT	1 cut(s)	1092
BisI	GCNGC	1 cut(s)	285
BlsI	GCNGC	1 cut(s)	286
BmcAI	AGTACT	1 cut(s)	1115
Bme1390I	CCNGG	2 cut(s)	420, 605
Bme18I	GGWCC	1 cut(s)	631
BmeT110I	CYCGRG	2 cut(s)	462, 945
BmgT120I	GGNCC	2 cut(s)	631, 949
BmiI	GGNNCC	5 cut(s)	6, 184, 633, 644, 950
BmrFI	CCNGG	2 cut(s)	420, 605
BmrI	ACTGGG	2 cut(s)	163, 169
BmsI	GCATC	3 cut(s)	93, 357, 807
BmuI	ACTGGG	2 cut(s)	163, 169
BpmI	CTGGAG	2 cut(s)	402, 626
Bpu10I	CCTNAGC	1 cut(s)	369
Bpu14I	TTCGAA	1 cut(s)	379
BpuEI	CTTGAG	1 cut(s)	407
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	2 cut(s)	79, 972
BsaXI	ACNNNNNCTCC	2 cut(s)	387, 417
Bsc4I	CCNNNNNNNGG	1 cut(s)	127
Bse118I	RCCGGY	1 cut(s)	185
Bse1I	ACTGG	3 cut(s)	169, 175, 285
BseBI	CCWGG	2 cut(s)	420, 605
BseDI	CCNNGG	2 cut(s)	79, 972
BseGI	GGATG	2 cut(s)	73, 117
BseLI	CCNNNNNNNGG	1 cut(s)	127
BseMII	CTCAG	1 cut(s)	360
BseNI	ACTGG	3 cut(s)	169, 175, 285
BseRI	GAGGAG	2 cut(s)	460, 838
BsgI	GTGCAG	1 cut(s)	924
Bsh1236I	CGCG	1 cut(s)	14
BshFI	GGCC	2 cut(s)	951, 1061
BshNI	GGYRCC	2 cut(s)	4, 182
BsiHKAI	GWGCWC	2 cut(s)	78, 393
BsiHKCI	CYCGRG	2 cut(s)	462, 945
BsiSI	CCGG	1 cut(s)	186
BslFI	GGGAC	2 cut(s)	157, 252
BslI	CCNNNNNNNGG	1 cut(s)	127
BsmAI	GTCTC	1 cut(s)	1095
BsmFI	GGGAC	2 cut(s)	157, 252
BsnI	GGCC	2 cut(s)	951, 1061
BsoBI	CYCGRG	2 cut(s)	462, 945
Bsp119I	TTCGAA	1 cut(s)	379
Bsp1286I	GDGCHC	3 cut(s)	78, 393, 946
Bsp143I	GATC	9 cut(s)	36, 438, 512, 523, 642, 736, 924, 988, 1092
Bsp68I	TCGCGA	1 cut(s)	14
BspACI	CCGC	6 cut(s)	114, 131, 213, 284, 582, 820
BspANI	GGCC	2 cut(s)	951, 1061
BspCNI	CTCAG	1 cut(s)	361
BspFNI	CGCG	1 cut(s)	14
BspHI	TCATGA	2 cut(s)	39, 595
BspLI	GGNNCC	5 cut(s)	6, 184, 633, 644, 950
BspMI	ACCTGC	1 cut(s)	955
BspPI	GGATC	3 cut(s)	531, 637, 650
BspT104I	TTCGAA	1 cut(s)	379
BspT107I	GGYRCC	2 cut(s)	4, 182
BspTI	CTTAAG	1 cut(s)	1096
BsrFI	RCCGGY	1 cut(s)	185
BsrI	ACTGG	3 cut(s)	169, 175, 285
BssAI	RCCGGY	1 cut(s)	185
BssECI	CCNNGG	2 cut(s)	79, 972
BssMI	GATC	9 cut(s)	36, 438, 512, 523, 642, 736, 924, 988, 1092
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	1 cut(s)	915
BssT1I	CCWWGG	2 cut(s)	79, 972
Bst2BI	CACGAG	1 cut(s)	915
Bst2UI	CCWGG	2 cut(s)	420, 605
Bst4CI	ACNGT	2 cut(s)	841, 1121
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	1 cut(s)	1096
BstBI	TTCGAA	1 cut(s)	379
BstC8I	GCNNGC	4 cut(s)	217, 342, 346, 855
BstDEI	CTNAG	1 cut(s)	369
BstF5I	GGATG	2 cut(s)	73, 117
BstFNI	CGCG	1 cut(s)	14
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	4 cut(s)	7, 141, 340, 1050
BstKTI	GATC	9 cut(s)	39, 441, 515, 526, 645, 739, 927, 991, 1095
BstMAI	GTCTC	1 cut(s)	1095
BstMBI	GATC	9 cut(s)	36, 438, 512, 523, 642, 736, 924, 988, 1092
BstMWI	GCNNNNNNNGC	3 cut(s)	11, 212, 354
BstNI	CCWGG	2 cut(s)	420, 605
BstNSI	RCATGY	3 cut(s)	344, 857, 865
BstSCI	CCNGG	2 cut(s)	418, 603
BstSFI	CTRYAG	2 cut(s)	541, 1117
BstUI	CGCG	1 cut(s)	14
BstX2I	RGATCY	2 cut(s)	642, 1092
BstYI	RGATCY	2 cut(s)	642, 1092
BsuRI	GGCC	2 cut(s)	951, 1061
BtsCI	GGATG	2 cut(s)	73, 117
BtsIMutI	CAGTG	1 cut(s)	930
BtuMI	TCGCGA	1 cut(s)	14
BveI	ACCTGC	1 cut(s)	955
Cac8I	GCNNGC	4 cut(s)	217, 342, 346, 855
CciI	TCATGA	2 cut(s)	39, 595
CfoI	GCGC	4 cut(s)	7, 141, 340, 1050
Cfr10I	RCCGGY	1 cut(s)	185
Cfr13I	GGNCC	2 cut(s)	631, 949
Csp6I	GTAC	5 cut(s)	100, 183, 459, 1114, 1122
CviQI	GTAC	5 cut(s)	100, 183, 459, 1114, 1122
DdeI	CTNAG	1 cut(s)	369
DinI	GGCGCC	1 cut(s)	6
DpnI	GATC	9 cut(s)	38, 440, 514, 525, 644, 738, 926, 990, 1094
DpnII	GATC	9 cut(s)	36, 438, 512, 523, 642, 736, 924, 988, 1092
EaeI	YGGCCR	1 cut(s)	1059
EciI	GGCGGA	1 cut(s)	103
Eco130I	CCWWGG	2 cut(s)	79, 972
Eco24I	GRGCYC	1 cut(s)	946
Eco32I	GATATC	2 cut(s)	548, 1087
Eco47I	GGWCC	1 cut(s)	631
Eco88I	CYCGRG	2 cut(s)	462, 945
EcoO109I	RGGNCCY	1 cut(s)	949
EcoRII	CCWGG	2 cut(s)	418, 603
EcoRV	GATATC	2 cut(s)	548, 1087
EcoT14I	CCWWGG	2 cut(s)	79, 972
EcoT22I	ATGCAT	1 cut(s)	855
EcoT38I	GRGCYC	1 cut(s)	946
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	2 cut(s)	79, 972
FaqI	GGGAC	2 cut(s)	157, 252
FbaI	TGATCA	2 cut(s)	438, 924
FblI	GTMKAC	1 cut(s)	228
Fnu4HI	GCNGC	1 cut(s)	285
FokI	GGATG	2 cut(s)	80, 104
FriOI	GRGCYC	1 cut(s)	946
Fsp4HI	GCNGC	1 cut(s)	285
FspBI	CTAG	1 cut(s)	273
GlaI	GCGC	4 cut(s)	6, 140, 339, 1049
GluI	GCNGC	1 cut(s)	285
GsuI	CTGGAG	2 cut(s)	402, 626
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	2 cut(s)	951, 1061
HapII	CCGG	1 cut(s)	186
HhaI	GCGC	4 cut(s)	7, 141, 340, 1050
Hin1I	GRCGYC	1 cut(s)	5
Hin6I	GCGC	4 cut(s)	5, 139, 338, 1048
HinP1I	GCGC	4 cut(s)	5, 139, 338, 1048
HincII	GTYRAC	2 cut(s)	229, 666
HindII	GTYRAC	2 cut(s)	229, 666
HindIII	AAGCTT	1 cut(s)	383
HinfI	GANTC	3 cut(s)	426, 491, 678
HpaII	CCGG	1 cut(s)	186
HphI	GGTGA	3 cut(s)	313, 919, 1166
Hpy166II	GTNNAC	2 cut(s)	229, 666
Hpy188I	TCNGA	6 cut(s)	22, 353, 496, 528, 1023, 1076
Hpy188III	TCNNGA	7 cut(s)	13, 40, 442, 596, 922, 1004, 1090
Hpy8I	GTNNAC	2 cut(s)	229, 666
Hpy99I	CGWCG	2 cut(s)	230, 491
HpyAV	CCTTC	2 cut(s)	58, 292
HpyCH4III	ACNGT	2 cut(s)	841, 1121
HpyCH4IV	ACGT	1 cut(s)	1149
HpyCH4V	TGCA	5 cut(s)	348, 780, 853, 857, 905
HpyF10VI	GCNNNNNNNGC	3 cut(s)	11, 212, 354
HpyF3I	CTNAG	1 cut(s)	369
HpySE526I	ACGT	1 cut(s)	1149
Hsp92I	GRCGYC	1 cut(s)	5
HspAI	GCGC	4 cut(s)	5, 139, 338, 1048
KasI	GGCGCC	1 cut(s)	4
KpnI	GGTACC	1 cut(s)	186
Ksp22I	TGATCA	2 cut(s)	438, 924
Kzo9I	GATC	9 cut(s)	36, 438, 512, 523, 642, 736, 924, 988, 1092
LmnI	GCTCC	1 cut(s)	81
LweI	GCATC	3 cut(s)	93, 357, 807
MaeI	CTAG	1 cut(s)	273
MaeII	ACGT	1 cut(s)	1149
MalI	GATC	9 cut(s)	38, 440, 514, 525, 644, 738, 926, 990, 1094
MboI	GATC	9 cut(s)	36, 438, 512, 523, 642, 736, 924, 988, 1092
MboII	GAAGA	4 cut(s)	542, 722, 837, 983
MfeI	CAATTG	1 cut(s)	312
MflI	RGATCY	2 cut(s)	642, 1092
MhlI	GDGCHC	3 cut(s)	78, 393, 946
MlsI	TGGCCA	1 cut(s)	1061
MluCI	AATT	6 cut(s)	312, 473, 576, 686, 719, 756
MluNI	TGGCCA	1 cut(s)	1061
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	1 cut(s)	435
Mox20I	TGGCCA	1 cut(s)	1061
Mph1103I	ATGCAT	1 cut(s)	855
MscI	TGGCCA	1 cut(s)	1061
MseI	TTAA	4 cut(s)	471, 506, 575, 1097
Msp20I	TGGCCA	1 cut(s)	1061
MspCI	CTTAAG	1 cut(s)	1096
MspI	CCGG	1 cut(s)	186
MspR9I	CCNGG	2 cut(s)	420, 605
MunI	CAATTG	1 cut(s)	312
MvaI	CCWGG	2 cut(s)	420, 605
MvnI	CGCG	1 cut(s)	14
MwoI	GCNNNNNNNGC	3 cut(s)	11, 212, 354
NarI	GGCGCC	1 cut(s)	5
NdeII	GATC	9 cut(s)	36, 438, 512, 523, 642, 736, 924, 988, 1092
NlaIV	GGNNCC	5 cut(s)	6, 184, 633, 644, 950
NmeAIII	GCCGAG	1 cut(s)	266
NruI	TCGCGA	1 cut(s)	14
NsiI	ATGCAT	1 cut(s)	855
NspI	RCATGY	3 cut(s)	344, 857, 865
NspV	TTCGAA	1 cut(s)	379
PaeI	GCATGC	2 cut(s)	344, 857
PaeR7I	CTCGAG	1 cut(s)	462
PagI	TCATGA	2 cut(s)	39, 595
PcsI	WCGNNNNNNNCGW	2 cut(s)	1027, 1036
PfeI	GAWTC	2 cut(s)	491, 678
PfoI	TCCNGGA	1 cut(s)	603
PkrI	GCNGC	1 cut(s)	286
PleI	GAGTC	1 cut(s)	434
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	1 cut(s)	434
Psp1406I	AACGTT	1 cut(s)	1149
Psp6I	CCWGG	2 cut(s)	418, 603
PspGI	CCWGG	2 cut(s)	418, 603
PspN4I	GGNNCC	5 cut(s)	6, 184, 633, 644, 950
PspPI	GGNCC	2 cut(s)	631, 949
PspXI	VCTCGAGB	1 cut(s)	462
PsuI	RGATCY	2 cut(s)	642, 1092
RruI	TCGCGA	1 cut(s)	14
RsaI	GTAC	5 cut(s)	101, 184, 460, 1115, 1123
RsaNI	GTAC	5 cut(s)	100, 183, 459, 1114, 1122
SalI	GTCGAC	1 cut(s)	227
SaqAI	TTAA	4 cut(s)	471, 506, 575, 1097
SatI	GCNGC	1 cut(s)	285
Sau3AI	GATC	9 cut(s)	36, 438, 512, 523, 642, 736, 924, 988, 1092
Sau96I	GGNCC	2 cut(s)	631, 949
ScaI	AGTACT	1 cut(s)	1115
SchI	GAGTC	1 cut(s)	435
ScrFI	CCNGG	2 cut(s)	420, 605
SduI	GDGCHC	3 cut(s)	78, 393, 946
SfaNI	GCATC	3 cut(s)	93, 357, 807
SfcI	CTRYAG	2 cut(s)	541, 1117
SfoI	GGCGCC	1 cut(s)	6
Sfr274I	CTCGAG	1 cut(s)	462
SfuI	TTCGAA	1 cut(s)	379
SinI	GGWCC	1 cut(s)	631
SlaI	CTCGAG	1 cut(s)	462
SmlI	CTYRAG	3 cut(s)	386, 462, 1096
SmoI	CTYRAG	3 cut(s)	386, 462, 1096
SphI	GCATGC	2 cut(s)	344, 857
Sse9I	AATT	6 cut(s)	312, 473, 576, 686, 719, 756
SsiI	CCGC	6 cut(s)	114, 131, 213, 284, 582, 820
SspDI	GGCGCC	1 cut(s)	4
SspMI	CTAG	1 cut(s)	273
StyD4I	CCNGG	2 cut(s)	418, 603
StyI	CCWWGG	2 cut(s)	79, 972
TaaI	ACNGT	2 cut(s)	841, 1121
TaiI	ACGT	1 cut(s)	1152
TaqI	TCGA	9 cut(s)	48, 147, 228, 379, 463, 486, 954, 1003, 1107
TasI	AATT	6 cut(s)	312, 473, 576, 686, 719, 756
TatI	WGTACW	1 cut(s)	1113
TauI	GCSGC	1 cut(s)	287
TfiI	GAWTC	2 cut(s)	491, 678
Tru1I	TTAA	4 cut(s)	471, 506, 575, 1097
Tru9I	TTAA	4 cut(s)	471, 506, 575, 1097
TscAI	CASTG	1 cut(s)	937
TspDTI	ATGAA	6 cut(s)	341, 389, 513, 612, 1007, 1160
TspGWI	ACGGA	3 cut(s)	308, 408, 644
TspRI	CASTG	1 cut(s)	937
Vha464I	CTTAAG	1 cut(s)	1096
VpaK11BI	GGWCC	1 cut(s)	631
XapI	RAATTY	1 cut(s)	719
XceI	RCATGY	3 cut(s)	344, 857, 865
XcmI	CCANNNNNNNNNTGG	1 cut(s)	277
XhoI	CTCGAG	1 cut(s)	462
XmiI	GTMKAC	1 cut(s)	228
XspI	CTAG	1 cut(s)	273
ZrmI	AGTACT	1 cut(s)	1115
Zsp2I	ATGCAT	1 cut(s)	855

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.