Eukaryotic initiation factor
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 39 |
| AciI | CCGC | 1 cut(s) | 43 |
| AclWI | GGATC | 3 cut(s) | 30, 125, 149 |
| AcsI | RAATTY | 1 cut(s) | 99 |
| AfaI | GTAC | 3 cut(s) | 437, 539, 547 |
| AflIII | ACRYGT | 1 cut(s) | 388 |
| AgsI | TTSAA | 4 cut(s) | 19, 203, 386, 533 |
| AluBI | AGCT | 3 cut(s) | 163, 266, 433 |
| AluI | AGCT | 3 cut(s) | 163, 266, 433 |
| Alw26I | GTCTC | 1 cut(s) | 519 |
| AlwI | GGATC | 3 cut(s) | 30, 125, 149 |
| Ama87I | CYCGRG | 2 cut(s) | 104, 369 |
| ApoI | RAATTY | 1 cut(s) | 99 |
| AvaI | CYCGRG | 2 cut(s) | 104, 369 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 529, 562 |
| BanII | GRGCYC | 1 cut(s) | 370 |
| BcoDI | GTCTC | 1 cut(s) | 519 |
| BfmI | CTRYAG | 1 cut(s) | 541 |
| BmcAI | AGTACT | 1 cut(s) | 539 |
| BmeT110I | CYCGRG | 2 cut(s) | 104, 369 |
| BmsI | GCATC | 3 cut(s) | 295, 384, 552 |
| Bsa29I | ATCGAT | 1 cut(s) | 378 |
| BseCI | ATCGAT | 1 cut(s) | 378 |
| BshVI | ATCGAT | 1 cut(s) | 378 |
| BsiHKCI | CYCGRG | 2 cut(s) | 104, 369 |
| BsmAI | GTCTC | 1 cut(s) | 519 |
| BsoBI | CYCGRG | 2 cut(s) | 104, 369 |
| Bsp1286I | GDGCHC | 1 cut(s) | 370 |
| Bsp1407I | TGTACA | 1 cut(s) | 435 |
| Bsp143I | GATC | 5 cut(s) | 22, 130, 141, 235, 412 |
| BspACI | CCGC | 1 cut(s) | 43 |
| BspDI | ATCGAT | 1 cut(s) | 378 |
| BspHI | TCATGA | 1 cut(s) | 123 |
| BspPI | GGATC | 3 cut(s) | 30, 125, 149 |
| BsrGI | TGTACA | 1 cut(s) | 435 |
| BssMI | GATC | 5 cut(s) | 22, 130, 141, 235, 412 |
| Bst4CI | ACNGT | 3 cut(s) | 332, 537, 545 |
| BstAUI | TGTACA | 1 cut(s) | 435 |
| BstKTI | GATC | 5 cut(s) | 25, 133, 144, 238, 415 |
| BstMAI | GTCTC | 1 cut(s) | 519 |
| BstMBI | GATC | 5 cut(s) | 22, 130, 141, 235, 412 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 160 |
| BstSFI | CTRYAG | 1 cut(s) | 541 |
| BstX2I | RGATCY | 1 cut(s) | 141 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 574 |
| BstYI | RGATCY | 1 cut(s) | 141 |
| Bsu15I | ATCGAT | 1 cut(s) | 378 |
| BsuTUI | ATCGAT | 1 cut(s) | 378 |
| BtsIMutI | CAGTG | 1 cut(s) | 337 |
| CciI | TCATGA | 1 cut(s) | 123 |
| ClaI | ATCGAT | 1 cut(s) | 378 |
| CseI | GACGC | 1 cut(s) | 480 |
| Csp6I | GTAC | 3 cut(s) | 436, 538, 546 |
| CviAII | CATG | 3 cut(s) | 56, 124, 568 |
| CviJI | RGCY | 6 cut(s) | 15, 163, 266, 368, 433, 578 |
| CviKI_1 | RGCY | 6 cut(s) | 15, 163, 266, 368, 433, 578 |
| CviQI | GTAC | 3 cut(s) | 436, 538, 546 |
| DpnI | GATC | 5 cut(s) | 24, 132, 143, 237, 414 |
| DpnII | GATC | 5 cut(s) | 22, 130, 141, 235, 412 |
| Eco24I | GRGCYC | 1 cut(s) | 370 |
| Eco32I | GATATC | 1 cut(s) | 511 |
| Eco88I | CYCGRG | 2 cut(s) | 104, 369 |
| EcoRV | GATATC | 1 cut(s) | 511 |
| EcoT38I | GRGCYC | 1 cut(s) | 370 |
| FaeI | CATG | 3 cut(s) | 59, 127, 571 |
| FaiI | YATR | 9 cut(s) | 57, 70, 125, 243, 284, 311, 411, 444, 569 |
| FatI | CATG | 3 cut(s) | 55, 123, 567 |
| FblI | GTMKAC | 1 cut(s) | 39 |
| FriOI | GRGCYC | 1 cut(s) | 370 |
| HgaI | GACGC | 1 cut(s) | 480 |
| Hin1II | CATG | 3 cut(s) | 59, 127, 571 |
| HinfI | GANTC | 3 cut(s) | 177, 335, 342 |
| Hpy166II | GTNNAC | 1 cut(s) | 40 |
| Hpy188I | TCNGA | 1 cut(s) | 27 |
| Hpy188III | TCNNGA | 7 cut(s) | 104, 106, 124, 339, 428, 499, 514 |
| Hpy8I | GTNNAC | 1 cut(s) | 40 |
| HpyCH4III | ACNGT | 3 cut(s) | 332, 537, 545 |
| HpyCH4IV | ACGT | 2 cut(s) | 36, 390 |
| HpyCH4V | TGCA | 1 cut(s) | 279 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 160 |
| HpySE526I | ACGT | 2 cut(s) | 36, 390 |
| Hsp92II | CATG | 3 cut(s) | 59, 127, 571 |
| Kzo9I | GATC | 5 cut(s) | 22, 130, 141, 235, 412 |
| LpnPI | CCDG | 3 cut(s) | 158, 499, 563 |
| LweI | GCATC | 3 cut(s) | 295, 384, 552 |
| MaeII | ACGT | 2 cut(s) | 36, 390 |
| MalI | GATC | 5 cut(s) | 24, 132, 143, 237, 414 |
| MboI | GATC | 5 cut(s) | 22, 130, 141, 235, 412 |
| MboII | GAAGA | 2 cut(s) | 221, 407 |
| MflI | RGATCY | 1 cut(s) | 141 |
| MhlI | GDGCHC | 1 cut(s) | 370 |
| MluCI | AATT | 3 cut(s) | 99, 185, 447 |
| MnlI | CCTC | 3 cut(s) | 5, 356, 365 |
| MseI | TTAA | 2 cut(s) | 324, 521 |
| MslI | CAYNNNNRTG | 1 cut(s) | 572 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 160 |
| NdeII | GATC | 5 cut(s) | 22, 130, 141, 235, 412 |
| NlaIII | CATG | 3 cut(s) | 59, 127, 571 |
| PaeR7I | CTCGAG | 2 cut(s) | 104, 369 |
| PagI | TCATGA | 1 cut(s) | 123 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 460 |
| PfeI | GAWTC | 3 cut(s) | 177, 335, 342 |
| PspXI | VCTCGAGB | 1 cut(s) | 369 |
| PsuI | RGATCY | 1 cut(s) | 141 |
| RsaI | GTAC | 3 cut(s) | 437, 539, 547 |
| RsaNI | GTAC | 3 cut(s) | 436, 538, 546 |
| RseI | CAYNNNNRTG | 1 cut(s) | 572 |
| SaqAI | TTAA | 2 cut(s) | 324, 521 |
| Sau3AI | GATC | 5 cut(s) | 22, 130, 141, 235, 412 |
| ScaI | AGTACT | 1 cut(s) | 539 |
| SduI | GDGCHC | 1 cut(s) | 370 |
| SetI | ASST | 6 cut(s) | 39, 165, 268, 393, 435, 481 |
| SfaNI | GCATC | 3 cut(s) | 295, 384, 552 |
| SfcI | CTRYAG | 1 cut(s) | 541 |
| Sfr274I | CTCGAG | 2 cut(s) | 104, 369 |
| SlaI | CTCGAG | 2 cut(s) | 104, 369 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 572 |
| SmlI | CTYRAG | 2 cut(s) | 104, 369 |
| SmoI | CTYRAG | 2 cut(s) | 104, 369 |
| Sse9I | AATT | 3 cut(s) | 99, 185, 447 |
| SsiI | CCGC | 1 cut(s) | 43 |
| TaaI | ACNGT | 3 cut(s) | 332, 537, 545 |
| TaiI | ACGT | 2 cut(s) | 39, 393 |
| TaqI | TCGA | 4 cut(s) | 105, 370, 378, 427 |
| TasI | AATT | 3 cut(s) | 99, 185, 447 |
| TatI | WGTACW | 2 cut(s) | 435, 537 |
| TfiI | GAWTC | 3 cut(s) | 177, 335, 342 |
| Tru1I | TTAA | 2 cut(s) | 324, 521 |
| Tru9I | TTAA | 2 cut(s) | 324, 521 |
| TscAI | CASTG | 1 cut(s) | 337 |
| TspDTI | ATGAA | 6 cut(s) | 72, 111, 112, 329, 431, 584 |
| TspRI | CASTG | 1 cut(s) | 337 |
| XapI | RAATTY | 1 cut(s) | 99 |
| XhoI | CTCGAG | 2 cut(s) | 104, 369 |
| XmiI | GTMKAC | 1 cut(s) | 39 |
| ZrmI | AGTACT | 1 cut(s) | 539 |