Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0000818

Physical Location & Seq

Genomic Coordinates Reverse (-)

63874213 .. 63875778

Gene Structure 1566 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000010175

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1128 bp

ATGGCAGCAACCGCAACCTCCAGGGCAAGCCAAACGATACGGGGTGTCAAAAACACCGTAGAGGAGAAGCTCAAGTTTGTGACCTCGGAATCTGAAGGTATAGAGCCGATAATGAGCTTCGACGATATGTGCCTTAAAGAAGAGCTCCTCCGAGGCATCTACAACCATGGCTTTGAGAAGCCCTCAGCTATCCAGCAACGGGCCATCAGACCCATTATTGAGCGCAGGGATGTTATCGCCCAGGCGCAGTCCGGTACTGGAAAGACATCAATGATTGCACTCGCCGCTTGCCAAATGGTTGACACCGCCTCCAGAGAGGTTCAGGCATTGATCCTTTCTCCAACGAGGGAATTGGCGGCCCAGACAGAGAAAGTGATATTGGCAATTGGAAATTACCTAAATATTCAAGTACATTCATGCATAGGAGGCAAAAGCTTGGGTGAAGATATCAGAAGGCTAGAGAACGGAGTTCATGTGGTGTCTGGAACTCCCGGTAGAGTCTGTGACATGCTCGACAGGAAAACTCTACGTCCTCGACAAATTAAATTATTAATTCTCGATGAATCTGATGAAATGTTGAGCAGAGGCTTCAAGGATCAGATTTACAACGTCTACCGCTATCTTTCGCATGAAGTCCAAGGAATCAAGCAATTTTTTGTTTCAGTTGAAAGAGAAGATTGGAAGTTTGATACTCTCTGTGATCTCTATGATACCCTTACGATTACACAAGCTGTTGTTTTCTGCAACACAAAGCGAAAGGTCGATTGGCTCGGCGAAAAGTTGCGGAGCAATAACTTCACTGTGTCTTCAATGCATGGAGACATGCCTCAGAAAGAGAGAGATGCTATAATGAAAGACTTTAAGAACAGTGAATCACGAATCCTCATCACAACAGATGTTTGGGCTCGAGGCATCGATGTTCAACACGTTTCGTTGGTTATCAACTATGATCTTCCAAACAATCGAGAGCTGTACATTCATAGAATTGGTCGTTCGGGTCGTTTTGGACGCAAGGGTGTGGCAATCAACTTTGTCAAGAACGATGATATTAGGATTTTAAGAGACATTGAACAGTACTACAGTACCCAGATTGATGAGATGCCCATGAATGTGGCTGATTTAATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009628 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0016020 GO:0016070 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035145 GO:0036293 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050896 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070482 GO:0071013 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03013 ko03015 ko03040 map03013 map03015 map03040

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

376

Amino Acids

42.7

Weight (kDa)

7.05

Isoelectric Point (pI)

39.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	61 - 190	3.4e-06	Type III restriction enzyme, res subunit
DEAD	PF00270	63 - 209	2.7e-38	DEAD/DEAH box helicase
Helicase_C	PF00271	228 - 336	2.8e-35	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000818)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51380 AT1G51380 AT1G51380 AT1G51380 AT3G19760
fragaria_vesca	FvH4_4g36800 FvH4_4g36800 FvH4_4g36800 FvH4_6g49811 FvH4_6g49900
malus_domestica	MD09G1053900.v1.1 MD09G1189300.v1.1 MD09G1268200.v1.1 MD09G1268600.v1.1 MD09G1268800.v1.1 MD13G1006800.v1.1 MD15G1056500.v1.1 MD16G1001000.v1.1
prunus_persica	Prupe.1G346100_v2.0.a1 Prupe.3G165200_v2.0.a1
pyrus_communis	pycom111g04510 pycom16g00440
rosa_chinensis	RchiOBHm_Chr0c44g0503461 RchiOBHm_Chr0c44g0503501 RchiOBHm_Chr0c44g0503531 RchiOBHm_Chr0c44g0503561 RchiOBHm_Chr0c44g0503691 RchiOBHm_Chr0c44g0503701 RchiOBHm_Chr0c44g0503721 RchiOBHm_Chr0c44g0503751 RchiOBHm_Chr2g0169961 RchiOBHm_Chr4g0386061 RchiOBHm_Chr4g0386101 RchiOBHm_Chr4g0386151 RchiOBHm_Chr4g0386181 RchiOBHm_Chr4g0386221 RchiOBHm_Chr4g0386241 RchiOBHm_Chr4g0386301 RchiOBHm_Chr4g0386341 RchiOBHm_Chr4g0386361 RchiOBHm_Chr4g0446531
rosa_laevigata	RLG00000005651 RLG00000010170 RLG00000010175 RLG00000021914
rosa_multiflora	Rmu_sc0005526.1_g000001 Rmu_sc0005795.1_g000003 Rmu_sc0013078.1_g000019 Rmu_sc0022031.1_g000001 Rmu_ssc0000389.1_g000007
rosa_roxburghii	Rroxscaffold_5G00333840 Rroxscaffold_5G00386950
rosa_rugosa	Rorug02G0544700 Rorug02G0545100 Rorug04G0372300
rosa_samantha	Rh2AG617000 Rh2AG617300 Rh2CG597900 Rh2DG639700 Rh2DG640100 Rh2DG640400 Rh4AG010700 Rh4AG433900 Rh4BG007600 Rh4BG443100 Rh4DG441700
rosa_wichuraiana	Rw2G051090 Rw4G037080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	612
AciI	CCGC	6 cut(s)	12, 285, 306, 356, 616, 784
AclWI	GGATC	2 cut(s)	325, 603
AcuI	CTGAAG	1 cut(s)	114
AfaI	GTAC	5 cut(s)	256, 411, 974, 1076, 1084
AfiI	CCNNNNNNNGG	1 cut(s)	199
AflIII	ACRYGT	1 cut(s)	925
AgsI	TTSAA	6 cut(s)	407, 592, 668, 810, 923, 1070
AjnI	CCWGG	2 cut(s)	20, 240
AjuI	GAANNNNNNNTTGG	4 cut(s)	362, 394, 748, 780
AluBI	AGCT	7 cut(s)	70, 117, 145, 188, 435, 731, 970
AluI	AGCT	7 cut(s)	70, 117, 145, 188, 435, 731, 970
Alw21I	GWGCWC	1 cut(s)	147
Alw26I	GTCTC	2 cut(s)	813, 1056
AlwI	GGATC	2 cut(s)	325, 603
Ama87I	CYCGRG	1 cut(s)	906
AoxI	GGCC	2 cut(s)	201, 357
ApeKI	GCWGC	1 cut(s)	5
AseI	ATTAAT	1 cut(s)	551
AspLEI	GCGC	2 cut(s)	225, 247
AspS9I	GGNCC	2 cut(s)	201, 358
AsuC2I	CCSGG	1 cut(s)	492
AsuHPI	GGTGA	1 cut(s)	452
AvaI	CYCGRG	1 cut(s)	906
BaeI	ACNNNNGTAYC	2 cut(s)	1066, 1099
BanII	GRGCYC	2 cut(s)	147, 907
BbsI	GAAGAC	1 cut(s)	798
Bbv12I	GWGCWC	1 cut(s)	147
BbvCI	CCTCAGC	1 cut(s)	184
BbvI	GCAGC	1 cut(s)	17
BccI	CCATC	1 cut(s)	212
BciT130I	CCWGG	2 cut(s)	22, 242
BcnI	CCSGG	1 cut(s)	492
BcoDI	GTCTC	2 cut(s)	813, 1056
BfaI	CTAG	1 cut(s)	458
BfmI	CTRYAG	1 cut(s)	1078
BisI	GCNGC	3 cut(s)	6, 285, 357
BlsI	GCNGC	3 cut(s)	7, 286, 358
BmcAI	AGTACT	1 cut(s)	1076
Bme1390I	CCNGG	3 cut(s)	22, 242, 492
BmeT110I	CYCGRG	1 cut(s)	906
BmgT120I	GGNCC	2 cut(s)	201, 358
BmrFI	CCNGG	3 cut(s)	22, 242, 492
BmsI	GCATC	4 cut(s)	165, 832, 921, 1089
BpiI	GAAGAC	1 cut(s)	798
BplI	GAGNNNNNCTC	2 cut(s)	167, 199
BpmI	CTGGAG	2 cut(s)	4, 295
Bpu10I	CCTNAGC	1 cut(s)	184
BpuEI	CTTGAG	1 cut(s)	56
BpuMI	CCSGG	1 cut(s)	492
Bsa29I	ATCGAT	1 cut(s)	915
BsaJI	CCNNGG	6 cut(s)	21, 84, 151, 166, 240, 637
BsaWI	WCCGGW	1 cut(s)	251
BsaXI	ACNNNNNCTCC	4 cut(s)	293, 323, 459, 489
Bsc4I	CCNNNNNNNGG	1 cut(s)	199
Bse1I	ACTGG	1 cut(s)	262
BseBI	CCWGG	2 cut(s)	22, 242
BseCI	ATCGAT	1 cut(s)	915
BseDI	CCNNGG	6 cut(s)	21, 84, 151, 166, 240, 637
BseGI	GGATG	1 cut(s)	235
BseLI	CCNNNNNNNGG	1 cut(s)	199
BseMII	CTCAG	2 cut(s)	198, 842
BseNI	ACTGG	1 cut(s)	262
BseRI	GAGGAG	2 cut(s)	77, 137
BseXI	GCAGC	1 cut(s)	17
BshFI	GGCC	2 cut(s)	203, 359
BshVI	ATCGAT	1 cut(s)	915
BsiHKAI	GWGCWC	1 cut(s)	147
BsiHKCI	CYCGRG	1 cut(s)	906
BsiSI	CCGG	2 cut(s)	252, 492
BslI	CCNNNNNNNGG	1 cut(s)	199
BsmAI	GTCTC	2 cut(s)	813, 1056
BsnI	GGCC	2 cut(s)	203, 359
BsoBI	CYCGRG	1 cut(s)	906
Bsp1286I	GDGCHC	2 cut(s)	147, 907
Bsp1407I	TGTACA	1 cut(s)	972
Bsp143I	GATC	4 cut(s)	330, 595, 700, 949
Bsp19I	CCATGG	1 cut(s)	166
BspACI	CCGC	6 cut(s)	12, 285, 306, 356, 616, 784
BspANI	GGCC	2 cut(s)	203, 359
BspCNI	CTCAG	2 cut(s)	197, 841
BspDI	ATCGAT	1 cut(s)	915
BspPI	GGATC	2 cut(s)	325, 603
BspQI	GCTCTTC	1 cut(s)	135
BsrGI	TGTACA	1 cut(s)	972
BsrI	ACTGG	1 cut(s)	262
BssECI	CCNNGG	6 cut(s)	21, 84, 151, 166, 240, 637
BssMI	GATC	4 cut(s)	330, 595, 700, 949
BssT1I	CCWWGG	2 cut(s)	166, 637
Bst2UI	CCWGG	2 cut(s)	22, 242
Bst4CI	ACNGT	5 cut(s)	58, 802, 869, 1074, 1082
Bst6I	CTCTTC	1 cut(s)	135
BstAUI	TGTACA	1 cut(s)	972
BstC8I	GCNNGC	2 cut(s)	28, 289
BstDEI	CTNAG	2 cut(s)	184, 828
BstDSI	CCRYGG	1 cut(s)	166
BstF5I	GGATG	1 cut(s)	235
BstHHI	GCGC	2 cut(s)	225, 247
BstKTI	GATC	4 cut(s)	333, 598, 703, 952
BstMAI	GTCTC	2 cut(s)	813, 1056
BstMBI	GATC	4 cut(s)	330, 595, 700, 949
BstMWI	GCNNNNNNNGC	3 cut(s)	11, 284, 426
BstNI	CCWGG	2 cut(s)	22, 242
BstNSI	RCATGY	2 cut(s)	511, 826
BstSCI	CCNGG	3 cut(s)	20, 240, 490
BstSFI	CTRYAG	1 cut(s)	1078
BstV1I	GCAGC	1 cut(s)	17
BstV2I	GAAGAC	1 cut(s)	798
BstXI	CCANNNNNNTGG	1 cut(s)	1111
Bsu15I	ATCGAT	1 cut(s)	915
BsuRI	GGCC	2 cut(s)	203, 359
BsuTUI	ATCGAT	1 cut(s)	915
BtgI	CCRYGG	1 cut(s)	166
BtsCI	GGATG	1 cut(s)	235
BtsIMutI	CAGTG	2 cut(s)	798, 874
Cac8I	GCNNGC	2 cut(s)	28, 289
CfoI	GCGC	2 cut(s)	225, 247
Cfr13I	GGNCC	2 cut(s)	201, 358
ClaI	ATCGAT	1 cut(s)	915
CseI	GACGC	1 cut(s)	1017
Csp6I	GTAC	5 cut(s)	255, 410, 973, 1075, 1083
CviAII	CATG	8 cut(s)	167, 417, 473, 508, 629, 815, 823, 1105
CviQI	GTAC	5 cut(s)	255, 410, 973, 1075, 1083
DdeI	CTNAG	2 cut(s)	184, 828
DpnI	GATC	4 cut(s)	332, 597, 702, 951
DpnII	GATC	4 cut(s)	330, 595, 700, 949
Eam1104I	CTCTTC	1 cut(s)	135
EarI	CTCTTC	1 cut(s)	135
Ecl136II	GAGCTC	1 cut(s)	145
Eco130I	CCWWGG	2 cut(s)	166, 637
Eco24I	GRGCYC	2 cut(s)	147, 907
Eco32I	GATATC	1 cut(s)	448
Eco53kI	GAGCTC	1 cut(s)	145
Eco57I	CTGAAG	1 cut(s)	114
Eco88I	CYCGRG	1 cut(s)	906
EcoICRI	GAGCTC	1 cut(s)	145
EcoRII	CCWGG	2 cut(s)	20, 240
EcoRV	GATATC	1 cut(s)	448
EcoT14I	CCWWGG	2 cut(s)	166, 637
EcoT22I	ATGCAT	2 cut(s)	422, 816
EcoT38I	GRGCYC	2 cut(s)	147, 907
ErhI	CCWWGG	2 cut(s)	166, 637
FaeI	CATG	8 cut(s)	170, 420, 476, 511, 632, 818, 826, 1108
FatI	CATG	8 cut(s)	166, 416, 472, 507, 628, 814, 822, 1104
FblI	GTMKAC	1 cut(s)	612
Fnu4HI	GCNGC	3 cut(s)	6, 285, 357
FokI	GGATG	1 cut(s)	242
FriOI	GRGCYC	2 cut(s)	147, 907
Fsp4HI	GCNGC	3 cut(s)	6, 285, 357
FspBI	CTAG	1 cut(s)	458
GlaI	GCGC	2 cut(s)	224, 246
GluI	GCNGC	3 cut(s)	6, 285, 357
GsuI	CTGGAG	2 cut(s)	4, 295
HaeIII	GGCC	2 cut(s)	203, 359
HapII	CCGG	2 cut(s)	252, 492
HgaI	GACGC	1 cut(s)	1017
HhaI	GCGC	2 cut(s)	225, 247
Hin1II	CATG	8 cut(s)	170, 420, 476, 511, 632, 818, 826, 1108
Hin6I	GCGC	2 cut(s)	223, 245
HinP1I	GCGC	2 cut(s)	223, 245
HincII	GTYRAC	1 cut(s)	301
HindII	GTYRAC	1 cut(s)	301
HindIII	AAGCTT	1 cut(s)	433
HinfI	GANTC	6 cut(s)	89, 498, 563, 642, 872, 879
HpaII	CCGG	2 cut(s)	252, 492
HphI	GGTGA	1 cut(s)	452
Hpy166II	GTNNAC	2 cut(s)	301, 613
Hpy188I	TCNGA	8 cut(s)	88, 94, 152, 209, 452, 568, 600, 831
Hpy188III	TCNNGA	6 cut(s)	312, 483, 557, 876, 965, 1036
Hpy8I	GTNNAC	2 cut(s)	301, 613
Hpy99I	CGWCG	1 cut(s)	125
HpyAV	CCTTC	2 cut(s)	89, 447
HpyCH4III	ACNGT	5 cut(s)	58, 802, 869, 1074, 1082
HpyCH4IV	ACGT	3 cut(s)	529, 609, 927
HpyCH4V	TGCA	4 cut(s)	278, 420, 744, 814
HpyF10VI	GCNNNNNNNGC	3 cut(s)	11, 284, 426
HpyF3I	CTNAG	2 cut(s)	184, 828
HpySE526I	ACGT	3 cut(s)	529, 609, 927
Hsp92II	CATG	8 cut(s)	170, 420, 476, 511, 632, 818, 826, 1108
HspAI	GCGC	2 cut(s)	223, 245
Kzo9I	GATC	4 cut(s)	330, 595, 700, 949
LguI	GCTCTTC	1 cut(s)	135
LmnI	GCTCC	2 cut(s)	150, 786
Lsp1109I	GCAGC	1 cut(s)	17
LweI	GCATC	4 cut(s)	165, 832, 921, 1089
MaeI	CTAG	1 cut(s)	458
MaeII	ACGT	3 cut(s)	529, 609, 927
MaeIII	GTNAC	2 cut(s)	79, 503
MalI	GATC	4 cut(s)	332, 597, 702, 951
MboI	GATC	4 cut(s)	330, 595, 700, 949
MboII	GAAGA	5 cut(s)	152, 455, 686, 798, 944
MfeI	CAATTG	1 cut(s)	384
MhlI	GDGCHC	2 cut(s)	147, 907
MluCI	AATT	8 cut(s)	350, 384, 391, 540, 545, 552, 650, 984
MlyI	GAGTC	1 cut(s)	507
MmeI	TCCRAC	1 cut(s)	365
Mph1103I	ATGCAT	2 cut(s)	422, 816
MseI	TTAA	6 cut(s)	135, 543, 551, 861, 1058, 1121
MslI	CAYNNNNRTG	1 cut(s)	1109
MspI	CCGG	2 cut(s)	252, 492
MspR9I	CCNGG	3 cut(s)	22, 242, 492
MunI	CAATTG	1 cut(s)	384
MvaI	CCWGG	2 cut(s)	22, 242
MwoI	GCNNNNNNNGC	3 cut(s)	11, 284, 426
NciI	CCSGG	1 cut(s)	492
NcoI	CCATGG	1 cut(s)	166
NdeII	GATC	4 cut(s)	330, 595, 700, 949
NlaIII	CATG	8 cut(s)	170, 420, 476, 511, 632, 818, 826, 1108
NmeAIII	GCCGAG	1 cut(s)	750
NmuCI	GTSAC	2 cut(s)	79, 503
NsiI	ATGCAT	2 cut(s)	422, 816
NspI	RCATGY	2 cut(s)	511, 826
PaeR7I	CTCGAG	1 cut(s)	906
PciSI	GCTCTTC	1 cut(s)	135
PcsI	WCGNNNNNNNCGW	1 cut(s)	997
PfeI	GAWTC	5 cut(s)	89, 563, 642, 872, 879
PkrI	GCNGC	3 cut(s)	7, 286, 358
PleI	GAGTC	1 cut(s)	506
PpsI	GAGTC	1 cut(s)	506
PshBI	ATTAAT	1 cut(s)	551
Psp124BI	GAGCTC	1 cut(s)	147
Psp6I	CCWGG	2 cut(s)	20, 240
PspGI	CCWGG	2 cut(s)	20, 240
PspPI	GGNCC	2 cut(s)	201, 358
PspXI	VCTCGAGB	1 cut(s)	906
RsaI	GTAC	5 cut(s)	256, 411, 974, 1076, 1084
RsaNI	GTAC	5 cut(s)	255, 410, 973, 1075, 1083
RseI	CAYNNNNRTG	1 cut(s)	1109
SacI	GAGCTC	1 cut(s)	147
SapI	GCTCTTC	1 cut(s)	135
SaqAI	TTAA	6 cut(s)	135, 543, 551, 861, 1058, 1121
SatI	GCNGC	3 cut(s)	6, 285, 357
Sau3AI	GATC	4 cut(s)	330, 595, 700, 949
Sau96I	GGNCC	2 cut(s)	201, 358
ScaI	AGTACT	1 cut(s)	1076
SchI	GAGTC	1 cut(s)	507
ScrFI	CCNGG	3 cut(s)	22, 242, 492
SduI	GDGCHC	2 cut(s)	147, 907
SfaNI	GCATC	4 cut(s)	165, 832, 921, 1089
SfcI	CTRYAG	1 cut(s)	1078
Sfr274I	CTCGAG	1 cut(s)	906
SlaI	CTCGAG	1 cut(s)	906
SmiMI	CAYNNNNRTG	1 cut(s)	1109
SmlI	CTYRAG	2 cut(s)	71, 906
SmoI	CTYRAG	2 cut(s)	71, 906
Sse9I	AATT	8 cut(s)	350, 384, 391, 540, 545, 552, 650, 984
SsiI	CCGC	6 cut(s)	12, 285, 306, 356, 616, 784
SspI	AATATT	1 cut(s)	403
SspMI	CTAG	1 cut(s)	458
SstI	GAGCTC	1 cut(s)	147
StyD4I	CCNGG	3 cut(s)	20, 240, 490
StyI	CCWWGG	2 cut(s)	166, 637
TaaI	ACNGT	5 cut(s)	58, 802, 869, 1074, 1082
TaiI	ACGT	3 cut(s)	532, 612, 930
TaqI	TCGA	8 cut(s)	120, 513, 535, 558, 762, 907, 915, 964
TasI	AATT	8 cut(s)	350, 384, 391, 540, 545, 552, 650, 984
TatI	WGTACW	3 cut(s)	409, 972, 1074
TauI	GCSGC	2 cut(s)	287, 359
TfiI	GAWTC	5 cut(s)	89, 563, 642, 872, 879
Tru1I	TTAA	6 cut(s)	135, 543, 551, 861, 1058, 1121
Tru9I	TTAA	6 cut(s)	135, 543, 551, 861, 1058, 1121
TscAI	CASTG	2 cut(s)	805, 874
TseFI	GTSAC	2 cut(s)	79, 503
TseI	GCWGC	1 cut(s)	5
Tsp45I	GTSAC	2 cut(s)	79, 503
TspDTI	ATGAA	8 cut(s)	405, 461, 576, 585, 645, 866, 968, 1121
TspGWI	ACGGA	1 cut(s)	480
TspRI	CASTG	2 cut(s)	805, 874
VspI	ATTAAT	1 cut(s)	551
XceI	RCATGY	2 cut(s)	511, 826
XhoI	CTCGAG	1 cut(s)	906
XmiI	GTMKAC	1 cut(s)	612
XspI	CTAG	1 cut(s)	458
ZrmI	AGTACT	1 cut(s)	1076
Zsp2I	ATGCAT	2 cut(s)	422, 816

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.