Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0000818

Physical Location & Seq

Genomic Coordinates Reverse (-)

59272 .. 62198

Gene Structure 2927 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1001000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1209 bp

ATGACAGAGGCATCGGCGAGACGCGGCGGAGGAATGGGTGACGAGAAGCTGGAGTTCGTAACTTCCGACGGGATTGAGCCCATCATGACCTTCGATCAGATGGGTTTAAGGGACGATCTCCTCCGAGGCGTCTACAACTACGGCTTTGAGAAGCCATCCGCCATCCAGCAACGAGCCGTCAGGCCCATCATTGAGGGCCGCGACGTCATCGCCCAGGCCCAGTCCGGTACCGGCAAGACCTCCATGATTGCCCTCACCGTCTGCCAGCTCGTCGACACCTCCTCCCGGGAGGTTCAGGCTTTGATATTGTCTCCGACGAGGGAATTGGCGGCGCAGACGGAGAAGGTGATATTAGCAATTGGGAATTTCATAAATATACAAGCTCATTCTTGCATTGGAGGCAAAAGCGTGGGTGAGGATATCCGAAAGCTCGAGTATGGCGTTCATGTGGTATCTGGAACTCCTGGCAGAGTCTGTGATATGATCAAAAGAAGAACTCTCCGAACTCGAGCAATCAAATTATTAGTTCTTGATGAATCTGATGAAATGTTGAGCAGAGGATTCAAGGATCAGATTTATGATGTCTACAGATATCTTCCTCCTGAGCTCCAGGTTTGCTTGATTTCTGCTACACTTCCTCATGAAATTTTGGAGATGACCAACAAGTTTATGACTGAACCTGTAAGGATCCTTGTTAAACGTGATGAGTTGACGTTGGAGGGAATCAAGCAATTTTTCGTGGCTGTCGAAAGAGAGGAATGGAAGTTTGATACTCTTTGTGATCTATATGATACCCTTACGATTACACAAGCTGTTATTTTCTGCAACACCAAGCGAAAGGTGGACTGGCTCACCGAAAAGATGAGGAGTAATAACTTTACTGTCTCTGCAATGCATGGAGATATGCCTCAGAAGGAAAGAGATGCTATTATGGCTGAGTTTCGTGGTGGTAATACTCGTGTCCTGATCACAACCGATGTTTGGGCCCGGGGCCTTGACGTTCAGCAGGTTTCTTTGGTTATCAATTATGATCTCCCAAACAATCGAGAGCTATACATTCATCGAATTGGTCGTTCTGGACGTTTTGGGCGCAAGGGTGTGGCAATAAACTTTGTGAAAAGTGATGATATTAAGATTCTAAGAGACATTGAGCAGTATTACAGTACGCAGATTGACGAAATGCCGATGAATGTTGCGGATTTGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009628 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0016020 GO:0016070 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035145 GO:0036293 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050896 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070482 GO:0071013 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03013 ko03015 ko03040 map03013 map03015 map03040

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

403

Amino Acids

45.57

Weight (kDa)

5.9

Isoelectric Point (pI)

39.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	54 - 216	6e-43	DEAD/DEAH box helicase
Helicase_C	PF00271	255 - 363	6.4e-35	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000818)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51380 AT1G51380 AT1G51380 AT1G51380 AT3G19760
fragaria_vesca	FvH4_4g36800 FvH4_4g36800 FvH4_4g36800 FvH4_6g49811 FvH4_6g49900
malus_domestica	MD09G1053900.v1.1 MD09G1189300.v1.1 MD09G1268200.v1.1 MD09G1268600.v1.1 MD09G1268800.v1.1 MD13G1006800.v1.1 MD15G1056500.v1.1 MD16G1001000.v1.1
prunus_persica	Prupe.1G346100_v2.0.a1 Prupe.3G165200_v2.0.a1
pyrus_communis	pycom111g04510 pycom16g00440
rosa_chinensis	RchiOBHm_Chr0c44g0503461 RchiOBHm_Chr0c44g0503501 RchiOBHm_Chr0c44g0503531 RchiOBHm_Chr0c44g0503561 RchiOBHm_Chr0c44g0503691 RchiOBHm_Chr0c44g0503701 RchiOBHm_Chr0c44g0503721 RchiOBHm_Chr0c44g0503751 RchiOBHm_Chr2g0169961 RchiOBHm_Chr4g0386061 RchiOBHm_Chr4g0386101 RchiOBHm_Chr4g0386151 RchiOBHm_Chr4g0386181 RchiOBHm_Chr4g0386221 RchiOBHm_Chr4g0386241 RchiOBHm_Chr4g0386301 RchiOBHm_Chr4g0386341 RchiOBHm_Chr4g0386361 RchiOBHm_Chr4g0446531
rosa_laevigata	RLG00000005651 RLG00000010170 RLG00000010175 RLG00000021914
rosa_multiflora	Rmu_sc0005526.1_g000001 Rmu_sc0005795.1_g000003 Rmu_sc0013078.1_g000019 Rmu_sc0022031.1_g000001 Rmu_ssc0000389.1_g000007
rosa_roxburghii	Rroxscaffold_5G00333840 Rroxscaffold_5G00386950
rosa_rugosa	Rorug02G0544700 Rorug02G0545100 Rorug04G0372300
rosa_samantha	Rh2AG617000 Rh2AG617300 Rh2CG597900 Rh2DG639700 Rh2DG640100 Rh2DG640400 Rh4AG010700 Rh4AG433900 Rh4BG007600 Rh4BG443100 Rh4DG441700
rosa_wichuraiana	Rw2G051090 Rw4G037080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	207
Acc36I	ACCTGC	1 cut(s)	997
Acc65I	GGTACC	1 cut(s)	227
AccB1I	GGYRCC	1 cut(s)	227
AccI	GTMKAC	3 cut(s)	132, 273, 585
AccII	CGCG	2 cut(s)	24, 201
AciI	CCGC	6 cut(s)	24, 27, 159, 199, 329, 1196
AclWI	GGATC	3 cut(s)	576, 682, 695
AcsI	RAATTY	2 cut(s)	364, 645
AcyI	GRCGYC	2 cut(s)	129, 204
AfaI	GTAC	2 cut(s)	229, 1165
AfiI	CCNNNNNNNGG	2 cut(s)	285, 981
AgsI	TTSAA	1 cut(s)	565
AjnI	CCWGG	3 cut(s)	213, 463, 609
AluBI	AGCT	7 cut(s)	49, 268, 383, 430, 607, 812, 1051
AluI	AGCT	7 cut(s)	49, 268, 383, 430, 607, 812, 1051
Alw21I	GWGCWC	1 cut(s)	609
Alw26I	GTCTC	4 cut(s)	13, 315, 889, 1137
AlwI	GGATC	3 cut(s)	576, 682, 695
AlwNI	CAGNNNCTG	1 cut(s)	474
Ama87I	CYCGRG	4 cut(s)	285, 431, 507, 987
AoxI	GGCC	5 cut(s)	182, 196, 216, 984, 991
ApaI	GGGCCC	1 cut(s)	988
ApoI	RAATTY	2 cut(s)	364, 645
Asp718I	GGTACC	1 cut(s)	227
AspLEI	GCGC	2 cut(s)	334, 1092
AspS9I	GGNCC	6 cut(s)	183, 196, 217, 984, 985, 991
AsuC2I	CCSGG	4 cut(s)	286, 287, 988, 989
AsuHPI	GGTGA	5 cut(s)	50, 247, 358, 425, 844
AvaI	CYCGRG	4 cut(s)	285, 431, 507, 987
BaeGI	GKGCMC	1 cut(s)	988
BamHI	GGATCC	1 cut(s)	687
BanI	GGYRCC	1 cut(s)	227
BanII	GRGCYC	3 cut(s)	81, 609, 988
BauI	CACGAG	1 cut(s)	957
Bbv12I	GWGCWC	1 cut(s)	609
BccI	CCATC	5 cut(s)	89, 94, 163, 170, 194
BceAI	ACGGC	2 cut(s)	157, 161
BciT130I	CCWGG	3 cut(s)	215, 465, 611
BclI	TGATCA	2 cut(s)	483, 966
BcnI	CCSGG	4 cut(s)	286, 287, 988, 989
BcoDI	GTCTC	4 cut(s)	13, 315, 889, 1137
BfmI	CTRYAG	1 cut(s)	586
BfuAI	ACCTGC	1 cut(s)	997
BisI	GCNGC	3 cut(s)	25, 199, 330
BlsI	GCNGC	3 cut(s)	26, 200, 331
Bme1390I	CCNGG	7 cut(s)	215, 286, 287, 465, 611, 988, 989
BmeT110I	CYCGRG	4 cut(s)	285, 431, 507, 987
BmgT120I	GGNCC	6 cut(s)	183, 196, 217, 984, 985, 991
BmiI	GGNNCC	4 cut(s)	229, 689, 986, 992
BmrFI	CCNGG	7 cut(s)	215, 286, 287, 465, 611, 988, 989
BmrI	ACTGGG	1 cut(s)	214
BmsI	GCATC	2 cut(s)	20, 913
BmuI	ACTGGG	1 cut(s)	214
BpmI	CTGGAG	2 cut(s)	71, 593
Bpu10I	CCTNAGC	1 cut(s)	603
BpuMI	CCSGG	4 cut(s)	286, 287, 988, 989
BsaHI	GRCGYC	2 cut(s)	129, 204
BsaJI	CCNNGG	5 cut(s)	124, 213, 285, 987, 988
BsaWI	WCCGGW	1 cut(s)	224
BsaXI	ACNNNNNCTCC	2 cut(s)	266, 296
Bsc4I	CCNNNNNNNGG	2 cut(s)	285, 981
Bse118I	RCCGGY	1 cut(s)	230
Bse1I	ACTGG	2 cut(s)	220, 851
Bse3DI	GCAATG	1 cut(s)	897
BseBI	CCWGG	3 cut(s)	215, 465, 611
BseDI	CCNNGG	5 cut(s)	124, 213, 285, 987, 988
BseGI	GGATG	2 cut(s)	155, 162
BseLI	CCNNNNNNNGG	2 cut(s)	285, 981
BseMI	GCAATG	1 cut(s)	897
BseMII	CTCAG	3 cut(s)	594, 923, 927
BseNI	ACTGG	2 cut(s)	220, 851
BseRI	GAGGAG	3 cut(s)	110, 271, 880
BseSI	GKGCMC	1 cut(s)	988
Bsh1236I	CGCG	2 cut(s)	24, 201
BshFI	GGCC	5 cut(s)	184, 198, 218, 986, 993
BshNI	GGYRCC	1 cut(s)	227
BsiHKAI	GWGCWC	1 cut(s)	609
BsiHKCI	CYCGRG	4 cut(s)	285, 431, 507, 987
BsiSI	CCGG	4 cut(s)	225, 231, 286, 988
BslFI	GGGAC	1 cut(s)	125
BslI	CCNNNNNNNGG	2 cut(s)	285, 981
BsmAI	GTCTC	4 cut(s)	13, 315, 889, 1137
BsmBI	CGTCTC	1 cut(s)	13
BsmFI	GGGAC	1 cut(s)	125
BsnI	GGCC	5 cut(s)	184, 198, 218, 986, 993
BsoBI	CYCGRG	4 cut(s)	285, 431, 507, 987
Bsp120I	GGGCCC	1 cut(s)	984
Bsp1286I	GDGCHC	3 cut(s)	81, 609, 988
Bsp143I	GATC	8 cut(s)	94, 115, 483, 568, 687, 781, 966, 1030
BspACI	CCGC	6 cut(s)	24, 27, 159, 199, 329, 1196
BspANI	GGCC	5 cut(s)	184, 198, 218, 986, 993
BspCNI	CTCAG	3 cut(s)	595, 922, 928
BspFNI	CGCG	2 cut(s)	24, 201
BspHI	TCATGA	2 cut(s)	84, 640
BspLI	GGNNCC	4 cut(s)	229, 689, 986, 992
BspMI	ACCTGC	1 cut(s)	997
BspPI	GGATC	3 cut(s)	576, 682, 695
BspT107I	GGYRCC	1 cut(s)	227
BsrDI	GCAATG	1 cut(s)	897
BsrFI	RCCGGY	1 cut(s)	230
BsrI	ACTGG	2 cut(s)	220, 851
BssAI	RCCGGY	1 cut(s)	230
BssECI	CCNNGG	5 cut(s)	124, 213, 285, 987, 988
BssMI	GATC	8 cut(s)	94, 115, 483, 568, 687, 781, 966, 1030
BssNI	GRCGYC	2 cut(s)	129, 204
BssSI	CACGAG	1 cut(s)	957
Bst2BI	CACGAG	1 cut(s)	957
Bst2UI	CCWGG	3 cut(s)	215, 465, 611
Bst4CI	ACNGT	3 cut(s)	259, 883, 1163
BstACI	GRCGYC	2 cut(s)	129, 204
BstC8I	GCNNGC	1 cut(s)	266
BstDEI	CTNAG	4 cut(s)	603, 909, 936, 1139
BstF5I	GGATG	2 cut(s)	155, 162
BstFNI	CGCG	2 cut(s)	24, 201
BstHHI	GCGC	2 cut(s)	334, 1092
BstKTI	GATC	8 cut(s)	97, 118, 486, 571, 690, 784, 969, 1033
BstMAI	GTCTC	4 cut(s)	13, 315, 889, 1137
BstMBI	GATC	8 cut(s)	94, 115, 483, 568, 687, 781, 966, 1030
BstMWI	GCNNNNNNNGC	2 cut(s)	399, 932
BstNI	CCWGG	3 cut(s)	215, 465, 611
BstSCI	CCNGG	7 cut(s)	213, 284, 285, 463, 609, 986, 987
BstSFI	CTRYAG	1 cut(s)	586
BstSLI	GKGCMC	1 cut(s)	988
BstUI	CGCG	2 cut(s)	24, 201
BstX2I	RGATCY	1 cut(s)	687
BstYI	RGATCY	1 cut(s)	687
BsuRI	GGCC	5 cut(s)	184, 198, 218, 986, 993
BtgZI	GCGATG	1 cut(s)	193
BtsCI	GGATG	2 cut(s)	155, 162
BveI	ACCTGC	1 cut(s)	997
Cac8I	GCNNGC	1 cut(s)	266
CaiI	CAGNNNCTG	1 cut(s)	474
CciI	TCATGA	2 cut(s)	84, 640
CfoI	GCGC	2 cut(s)	334, 1092
Cfr10I	RCCGGY	1 cut(s)	230
Cfr13I	GGNCC	6 cut(s)	183, 196, 217, 984, 985, 991
Cfr9I	CCCGGG	2 cut(s)	285, 987
CseI	GACGC	2 cut(s)	30, 118
Csp6I	GTAC	2 cut(s)	228, 1164
CviAII	CATG	5 cut(s)	85, 244, 446, 641, 896
CviQI	GTAC	2 cut(s)	228, 1164
DdeI	CTNAG	4 cut(s)	603, 909, 936, 1139
DpnI	GATC	8 cut(s)	96, 117, 485, 570, 689, 783, 968, 1032
DpnII	GATC	8 cut(s)	94, 115, 483, 568, 687, 781, 966, 1030
EciI	GGCGGA	2 cut(s)	42, 148
Ecl136II	GAGCTC	1 cut(s)	607
Eco24I	GRGCYC	3 cut(s)	81, 609, 988
Eco32I	GATATC	2 cut(s)	421, 593
Eco53kI	GAGCTC	1 cut(s)	607
Eco88I	CYCGRG	4 cut(s)	285, 431, 507, 987
EcoICRI	GAGCTC	1 cut(s)	607
EcoO109I	RGGNCCY	1 cut(s)	991
EcoRII	CCWGG	3 cut(s)	213, 463, 609
EcoRV	GATATC	2 cut(s)	421, 593
EcoT22I	ATGCAT	1 cut(s)	897
EcoT38I	GRGCYC	3 cut(s)	81, 609, 988
Esp3I	CGTCTC	1 cut(s)	13
FaeI	CATG	5 cut(s)	88, 247, 449, 644, 899
FaqI	GGGAC	1 cut(s)	125
FatI	CATG	5 cut(s)	84, 243, 445, 640, 895
FbaI	TGATCA	2 cut(s)	483, 966
FblI	GTMKAC	3 cut(s)	132, 273, 585
Fnu4HI	GCNGC	3 cut(s)	25, 199, 330
FokI	GGATG	2 cut(s)	142, 149
FriOI	GRGCYC	3 cut(s)	81, 609, 988
Fsp4HI	GCNGC	3 cut(s)	25, 199, 330
GlaI	GCGC	2 cut(s)	333, 1091
GluI	GCNGC	3 cut(s)	25, 199, 330
GsuI	CTGGAG	2 cut(s)	71, 593
HaeIII	GGCC	5 cut(s)	184, 198, 218, 986, 993
HapII	CCGG	4 cut(s)	225, 231, 286, 988
HgaI	GACGC	2 cut(s)	30, 118
HhaI	GCGC	2 cut(s)	334, 1092
Hin1I	GRCGYC	2 cut(s)	129, 204
Hin1II	CATG	5 cut(s)	88, 247, 449, 644, 899
Hin6I	GCGC	2 cut(s)	332, 1090
HinP1I	GCGC	2 cut(s)	332, 1090
HincII	GTYRAC	2 cut(s)	274, 711
HindII	GTYRAC	2 cut(s)	274, 711
HinfI	GANTC	5 cut(s)	471, 536, 561, 723, 1135
HpaII	CCGG	4 cut(s)	225, 231, 286, 988
HphI	GGTGA	5 cut(s)	50, 247, 358, 425, 844
Hpy166II	GTNNAC	5 cut(s)	133, 274, 586, 711, 844
Hpy188I	TCNGA	9 cut(s)	67, 99, 125, 315, 425, 503, 541, 573, 912
Hpy188III	TCNNGA	8 cut(s)	85, 456, 530, 602, 641, 964, 1046, 1077
Hpy8I	GTNNAC	5 cut(s)	133, 274, 586, 711, 844
Hpy99I	CGWCG	4 cut(s)	71, 206, 275, 319
HpyAV	CCTTC	3 cut(s)	100, 337, 907
HpyCH4III	ACNGT	3 cut(s)	259, 883, 1163
HpyCH4IV	ACGT	5 cut(s)	204, 700, 713, 999, 1081
HpyCH4V	TGCA	4 cut(s)	393, 825, 890, 895
HpyF10VI	GCNNNNNNNGC	2 cut(s)	399, 932
HpyF3I	CTNAG	4 cut(s)	603, 909, 936, 1139
HpySE526I	ACGT	5 cut(s)	204, 700, 713, 999, 1081
Hsp92I	GRCGYC	2 cut(s)	129, 204
Hsp92II	CATG	5 cut(s)	88, 247, 449, 644, 899
HspAI	GCGC	2 cut(s)	332, 1090
KpnI	GGTACC	1 cut(s)	231
Ksp22I	TGATCA	2 cut(s)	483, 966
Kzo9I	GATC	8 cut(s)	94, 115, 483, 568, 687, 781, 966, 1030
LmnI	GCTCC	1 cut(s)	612
LweI	GCATC	2 cut(s)	20, 913
MaeII	ACGT	5 cut(s)	204, 700, 713, 999, 1081
MaeIII	GTNAC	2 cut(s)	38, 58
MalI	GATC	8 cut(s)	96, 117, 485, 570, 689, 783, 968, 1032
MboI	GATC	8 cut(s)	94, 115, 483, 568, 687, 781, 966, 1030
MboII	GAAGA	2 cut(s)	504, 587
MfeI	CAATTG	1 cut(s)	357
MflI	RGATCY	1 cut(s)	687
MhlI	GDGCHC	3 cut(s)	81, 609, 988
MluCI	AATT	8 cut(s)	323, 357, 364, 518, 645, 731, 1024, 1065
MlyI	GAGTC	1 cut(s)	480
MmeI	TCCRAC	3 cut(s)	90, 338, 696
Mph1103I	ATGCAT	1 cut(s)	897
MseI	TTAA	3 cut(s)	107, 696, 1131
MspI	CCGG	4 cut(s)	225, 231, 286, 988
MspR9I	CCNGG	7 cut(s)	215, 286, 287, 465, 611, 988, 989
MunI	CAATTG	1 cut(s)	357
MvaI	CCWGG	3 cut(s)	215, 465, 611
MvnI	CGCG	2 cut(s)	24, 201
MwoI	GCNNNNNNNGC	2 cut(s)	399, 932
NciI	CCSGG	4 cut(s)	286, 287, 988, 989
NdeII	GATC	8 cut(s)	94, 115, 483, 568, 687, 781, 966, 1030
NlaIII	CATG	5 cut(s)	88, 247, 449, 644, 899
NlaIV	GGNNCC	4 cut(s)	229, 689, 986, 992
NmuCI	GTSAC	1 cut(s)	38
NsiI	ATGCAT	1 cut(s)	897
PaeR7I	CTCGAG	2 cut(s)	431, 507
PagI	TCATGA	2 cut(s)	84, 640
PcsI	WCGNNNNNNNCGW	5 cut(s)	63, 438, 744, 1069, 1078
PfeI	GAWTC	4 cut(s)	536, 561, 723, 1135
PkrI	GCNGC	3 cut(s)	26, 200, 331
PleI	GAGTC	1 cut(s)	479
PpsI	GAGTC	1 cut(s)	479
Psp124BI	GAGCTC	1 cut(s)	609
Psp6I	CCWGG	3 cut(s)	213, 463, 609
PspGI	CCWGG	3 cut(s)	213, 463, 609
PspN4I	GGNNCC	4 cut(s)	229, 689, 986, 992
PspOMI	GGGCCC	1 cut(s)	984
PspPI	GGNCC	6 cut(s)	183, 196, 217, 984, 985, 991
PspXI	VCTCGAGB	2 cut(s)	431, 507
PstNI	CAGNNNCTG	1 cut(s)	474
PsuI	RGATCY	1 cut(s)	687
RsaI	GTAC	2 cut(s)	229, 1165
RsaNI	GTAC	2 cut(s)	228, 1164
SacI	GAGCTC	1 cut(s)	609
SalI	GTCGAC	1 cut(s)	272
SaqAI	TTAA	3 cut(s)	107, 696, 1131
SatI	GCNGC	3 cut(s)	25, 199, 330
Sau3AI	GATC	8 cut(s)	94, 115, 483, 568, 687, 781, 966, 1030
Sau96I	GGNCC	6 cut(s)	183, 196, 217, 984, 985, 991
SchI	GAGTC	1 cut(s)	480
ScrFI	CCNGG	7 cut(s)	215, 286, 287, 465, 611, 988, 989
SduI	GDGCHC	3 cut(s)	81, 609, 988
SfaNI	GCATC	2 cut(s)	20, 913
SfcI	CTRYAG	1 cut(s)	586
Sfr274I	CTCGAG	2 cut(s)	431, 507
SlaI	CTCGAG	2 cut(s)	431, 507
SmaI	CCCGGG	2 cut(s)	287, 989
SmlI	CTYRAG	2 cut(s)	431, 507
SmoI	CTYRAG	2 cut(s)	431, 507
Sse9I	AATT	8 cut(s)	323, 357, 364, 518, 645, 731, 1024, 1065
SsiI	CCGC	6 cut(s)	24, 27, 159, 199, 329, 1196
SstI	GAGCTC	1 cut(s)	609
StyD4I	CCNGG	7 cut(s)	213, 284, 285, 463, 609, 986, 987
TaaI	ACNGT	3 cut(s)	259, 883, 1163
TaiI	ACGT	5 cut(s)	207, 703, 716, 1002, 1084
TaqI	TCGA	7 cut(s)	93, 273, 432, 508, 747, 1045, 1063
TasI	AATT	8 cut(s)	323, 357, 364, 518, 645, 731, 1024, 1065
TauI	GCSGC	3 cut(s)	27, 201, 332
TfiI	GAWTC	4 cut(s)	536, 561, 723, 1135
Tru1I	TTAA	3 cut(s)	107, 696, 1131
Tru9I	TTAA	3 cut(s)	107, 696, 1131
TseFI	GTSAC	1 cut(s)	38
Tsp45I	GTSAC	1 cut(s)	38
TspDTI	ATGAA	7 cut(s)	358, 434, 549, 558, 657, 1049, 1202
TspGWI	ACGGA	1 cut(s)	353
TspMI	CCCGGG	2 cut(s)	285, 987
XapI	RAATTY	2 cut(s)	364, 645
XcmI	CCANNNNNNNNNTGG	1 cut(s)	838
XhoI	CTCGAG	2 cut(s)	431, 507
XmaI	CCCGGG	2 cut(s)	285, 987
XmiI	GTMKAC	3 cut(s)	132, 273, 585
ZraI	GACGTC	1 cut(s)	205
Zsp2I	ATGCAT	1 cut(s)	897

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.