Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0000818

Physical Location & Seq

Genomic Coordinates Forward (+)

79274074 .. 79275793

Gene Structure 1720 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021914

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1245 bp

ATGGCAGCAATAGCAACCGCGAGAGCGAGCGTGGTTCCGATGAACCGATCGGTTCAGGGCGGCAGCAAGGTGCTGAAGGAAATGCTGGAGTTTGTGCCGTCGGATGATATAGAGCCGATCATGAGCTTCGATCAGATGAACCTCAAGGACGAGCTACTCCGAGGCATCTACAACTACGGCTTCGAGAAGCCCTCCGCCATCCAGCAAAGGGCCATCAGACCCATAATCGAGGGCCGCGACGTCATAGCTCAGGCTCAATCTGGCACTGGAAAGACCTCCATGATTGCCCTAGCTTCTTGCCAAACCGTCGACACCTCCTCCAGAGAGGTTCAGGTGTTGATATTGTCTCCAACGAGGGAGTTGGCGGCACAGACAGAGAAAGTGATACTGGCAATGGGAAACTACATGAGTATTCAAGCGCATTCCTGCATTGGAGGCAAAAGTGTGGGTGAGGATATCAGAAAGCTAGAGAACGGAGTTCAGGTGGTCTCTGGAACTCCTGGTAGAGTCCATGACATGATTCAGAGGGGAAGTCTACGCACTCGACACATCAAACTACTAATTCTTGATGAATCTGATGAGATGTTGAGCAGAGGCTTCAAGAAGAAAATTTACGAAGTCTACAGACATCTACAAGATACCCTACAAGTTTGCTTGATCTCCGCGACGCTCCCTAATGAAATTTTGGAGATGACCAACAAGTTCATGACCGATCCTATAAGGATCCTGGTTAAACGTGACGAGCTGACTTTGGAGGGAATCAAGCAATTTTTCGTTGCAGTTGAAGAAGAAGATTGGAAGTTTGATACTTTGTGCGATCTCTATGATACCCTTACGATCACACAAGCTGTTATTTTCTGCAACACAAGGCGAAAAGTGGACTGGCTTACTGAAAAGATGCGGACTAGTAACTTTGCTGTGTCTGCAATGCATGGAGACATGCCTCAGAAGGAGAGAGATGCTATTATGAAGGAGTTTCAGAAAGGTCTTTCACGAGTCCTCATCACAACTGATGTTTGGGCTCGGGGCATCGATGTTCAGCAGGTTTCGCTCGTTATCAACTACGATCTTCCAAACAATCGAGAGCTATACATTCATAGGATTGGTCGTTCAGGTCGATTTGGACGAAAGGGTGTGGCAATAAACTTTGTCAAGAACGATGATATCAGGATCTTACGAGACATTGAACAGTACTACAGTACCCAGATTGACGAGATGCCCATGAATGTGGCTGATTTACTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009628 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0016020 GO:0016070 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035145 GO:0036293 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050896 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0070482 GO:0071013 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03013 ko03015 ko03040 map03013 map03015 map03040

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

415

Amino Acids

46.96

Weight (kDa)

6.37

Isoelectric Point (pI)

39.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	64 - 213	5.7e-07	Type III restriction enzyme, res subunit
DEAD	PF00270	66 - 228	1.2e-43	DEAD/DEAH box helicase
Helicase_C	PF00271	267 - 375	2.5e-34	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000818)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51380 AT1G51380 AT1G51380 AT1G51380 AT3G19760
fragaria_vesca	FvH4_4g36800 FvH4_4g36800 FvH4_4g36800 FvH4_6g49811 FvH4_6g49900
malus_domestica	MD09G1053900.v1.1 MD09G1189300.v1.1 MD09G1268200.v1.1 MD09G1268600.v1.1 MD09G1268800.v1.1 MD13G1006800.v1.1 MD15G1056500.v1.1 MD16G1001000.v1.1
prunus_persica	Prupe.1G346100_v2.0.a1 Prupe.3G165200_v2.0.a1
pyrus_communis	pycom111g04510 pycom16g00440
rosa_chinensis	RchiOBHm_Chr0c44g0503461 RchiOBHm_Chr0c44g0503501 RchiOBHm_Chr0c44g0503531 RchiOBHm_Chr0c44g0503561 RchiOBHm_Chr0c44g0503691 RchiOBHm_Chr0c44g0503701 RchiOBHm_Chr0c44g0503721 RchiOBHm_Chr0c44g0503751 RchiOBHm_Chr2g0169961 RchiOBHm_Chr4g0386061 RchiOBHm_Chr4g0386101 RchiOBHm_Chr4g0386151 RchiOBHm_Chr4g0386181 RchiOBHm_Chr4g0386221 RchiOBHm_Chr4g0386241 RchiOBHm_Chr4g0386301 RchiOBHm_Chr4g0386341 RchiOBHm_Chr4g0386361 RchiOBHm_Chr4g0446531
rosa_laevigata	RLG00000005651 RLG00000010170 RLG00000010175 RLG00000021914
rosa_multiflora	Rmu_sc0005526.1_g000001 Rmu_sc0005795.1_g000003 Rmu_sc0013078.1_g000019 Rmu_sc0022031.1_g000001 Rmu_ssc0000389.1_g000007
rosa_roxburghii	Rroxscaffold_5G00333840 Rroxscaffold_5G00386950
rosa_rugosa	Rorug02G0544700 Rorug02G0545100 Rorug04G0372300
rosa_samantha	Rh2AG617000 Rh2AG617300 Rh2CG597900 Rh2DG639700 Rh2DG640100 Rh2DG640400 Rh4AG010700 Rh4AG433900 Rh4BG007600 Rh4BG443100 Rh4DG441700
rosa_wichuraiana	Rw2G051090 Rw4G037080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	243
Acc36I	ACCTGC	1 cut(s)	1033
AccI	GTMKAC	3 cut(s)	309, 535, 621
AccII	CGCG	3 cut(s)	20, 237, 665
AciI	CCGC	7 cut(s)	18, 60, 195, 235, 365, 663, 901
AclWI	GGATC	4 cut(s)	707, 718, 731, 1178
AcsI	RAATTY	2 cut(s)	609, 681
AcuI	CTGAAG	1 cut(s)	95
AcyI	GRCGYC	1 cut(s)	240
AfaI	GTAC	2 cut(s)	1193, 1201
AfiI	CCNNNNNNNGG	1 cut(s)	208
AgsI	TTSAA	4 cut(s)	416, 601, 785, 1187
AhlI	ACTAGT	1 cut(s)	905
AjnI	CCWGG	2 cut(s)	499, 726
AloI	GAACNNNNNNTCC	2 cut(s)	1093, 1125
AluBI	AGCT	8 cut(s)	126, 154, 248, 293, 466, 745, 848, 1087
AluI	AGCT	8 cut(s)	126, 154, 248, 293, 466, 745, 848, 1087
Alw26I	GTCTC	4 cut(s)	351, 493, 930, 1173
AlwI	GGATC	4 cut(s)	707, 718, 731, 1178
Ama87I	CYCGRG	1 cut(s)	1023
AoxI	GGCC	2 cut(s)	210, 232
ApeKI	GCWGC	2 cut(s)	5, 63
ApoI	RAATTY	2 cut(s)	609, 681
AspLEI	GCGC	1 cut(s)	421
AspS9I	GGNCC	2 cut(s)	210, 232
AsuHPI	GGTGA	1 cut(s)	461
AvaI	CYCGRG	1 cut(s)	1023
BaeI	ACNNNNGTAYC	2 cut(s)	1183, 1216
BamHI	GGATCC	1 cut(s)	723
BanII	GRGCYC	1 cut(s)	1024
BarI	GAAGNNNNNNTAC	2 cut(s)	596, 628
BauI	CACGAG	1 cut(s)	993
BbvI	GCAGC	2 cut(s)	17, 75
BccI	CCATC	2 cut(s)	206, 221
BceAI	ACGGC	2 cut(s)	82, 193
BciT130I	CCWGG	2 cut(s)	501, 728
BcoDI	GTCTC	4 cut(s)	351, 493, 930, 1173
BcuI	ACTAGT	1 cut(s)	905
BfaI	CTAG	3 cut(s)	290, 467, 906
BfmI	CTRYAG	2 cut(s)	622, 1195
BfuAI	ACCTGC	1 cut(s)	1033
BisI	GCNGC	5 cut(s)	6, 61, 64, 235, 366
BlsI	GCNGC	5 cut(s)	7, 62, 65, 236, 367
BmcAI	AGTACT	1 cut(s)	1193
Bme1390I	CCNGG	2 cut(s)	501, 728
BmeT110I	CYCGRG	1 cut(s)	1023
BmgT120I	GGNCC	2 cut(s)	210, 232
BmiI	GGNNCC	2 cut(s)	36, 725
BmrFI	CCNGG	2 cut(s)	501, 728
BmsI	GCATC	5 cut(s)	174, 888, 949, 1038, 1206
BplI	GAGNNNNNCTC	2 cut(s)	176, 208
BpmI	CTGGAG	2 cut(s)	107, 304
Bpu10I	CCTNAGC	1 cut(s)	249
BpuEI	CTTGAG	1 cut(s)	128
Bsa29I	ATCGAT	1 cut(s)	1032
BsaHI	GRCGYC	1 cut(s)	240
BsaI	GGTCTC	1 cut(s)	493
BsaJI	CCNNGG	1 cut(s)	160
BsaXI	ACNNNNNCTCC	4 cut(s)	302, 332, 468, 498
Bsc4I	CCNNNNNNNGG	1 cut(s)	208
Bse1I	ACTGG	3 cut(s)	271, 393, 887
Bse3DI	GCAATG	2 cut(s)	399, 933
BseBI	CCWGG	2 cut(s)	501, 728
BseCI	ATCGAT	1 cut(s)	1032
BseDI	CCNNGG	1 cut(s)	160
BseGI	GGATG	2 cut(s)	109, 198
BseLI	CCNNNNNNNGG	1 cut(s)	208
BseMI	GCAATG	2 cut(s)	399, 933
BseMII	CTCAG	2 cut(s)	263, 959
BseNI	ACTGG	3 cut(s)	271, 393, 887
BseRI	GAGGAG	1 cut(s)	307
BseXI	GCAGC	2 cut(s)	17, 75
Bsh1236I	CGCG	3 cut(s)	20, 237, 665
Bsh1285I	CGRYCG	1 cut(s)	50
BshFI	GGCC	2 cut(s)	212, 234
BshVI	ATCGAT	1 cut(s)	1032
BsiEI	CGRYCG	1 cut(s)	50
BsiHKCI	CYCGRG	1 cut(s)	1023
BslI	CCNNNNNNNGG	1 cut(s)	208
BsmAI	GTCTC	4 cut(s)	351, 493, 930, 1173
BsmI	GAATGC	1 cut(s)	421
BsnI	GGCC	2 cut(s)	212, 234
Bso31I	GGTCTC	1 cut(s)	493
BsoBI	CYCGRG	1 cut(s)	1023
Bsp1286I	GDGCHC	1 cut(s)	1024
BspACI	CCGC	7 cut(s)	18, 60, 195, 235, 365, 663, 901
BspANI	GGCC	2 cut(s)	212, 234
BspCNI	CTCAG	2 cut(s)	262, 958
BspDI	ATCGAT	1 cut(s)	1032
BspFNI	CGCG	3 cut(s)	20, 237, 665
BspHI	TCATGA	2 cut(s)	120, 705
BspLI	GGNNCC	2 cut(s)	36, 725
BspMI	ACCTGC	1 cut(s)	1033
BspPI	GGATC	4 cut(s)	707, 718, 731, 1178
BspTNI	GGTCTC	1 cut(s)	493
BsrDI	GCAATG	2 cut(s)	399, 933
BsrI	ACTGG	3 cut(s)	271, 393, 887
BssECI	CCNNGG	1 cut(s)	160
BssNI	GRCGYC	1 cut(s)	240
BssSI	CACGAG	1 cut(s)	993
Bst2BI	CACGAG	1 cut(s)	993
Bst2UI	CCWGG	2 cut(s)	501, 728
Bst4CI	ACNGT	3 cut(s)	307, 1191, 1199
BstACI	GRCGYC	1 cut(s)	240
BstC8I	GCNNGC	1 cut(s)	28
BstDEI	CTNAG	2 cut(s)	249, 945
BstF5I	GGATG	2 cut(s)	109, 198
BstFNI	CGCG	3 cut(s)	20, 237, 665
BstHHI	GCGC	1 cut(s)	421
BstMAI	GTCTC	4 cut(s)	351, 493, 930, 1173
BstMCI	CGRYCG	1 cut(s)	50
BstMWI	GCNNNNNNNGC	4 cut(s)	11, 435, 923, 1048
BstNI	CCWGG	2 cut(s)	501, 728
BstNSI	RCATGY	1 cut(s)	943
BstSCI	CCNGG	2 cut(s)	499, 726
BstSFI	CTRYAG	2 cut(s)	622, 1195
BstUI	CGCG	3 cut(s)	20, 237, 665
BstV1I	GCAGC	2 cut(s)	17, 75
BstX2I	RGATCY	2 cut(s)	723, 1170
BstXI	CCANNNNNNTGG	1 cut(s)	1228
BstYI	RGATCY	2 cut(s)	723, 1170
Bsu15I	ATCGAT	1 cut(s)	1032
BsuRI	GGCC	2 cut(s)	212, 234
BsuTUI	ATCGAT	1 cut(s)	1032
BtsCI	GGATG	2 cut(s)	109, 198
BtsIMutI	CAGTG	1 cut(s)	264
BveI	ACCTGC	1 cut(s)	1033
Cac8I	GCNNGC	1 cut(s)	28
CciI	TCATGA	2 cut(s)	120, 705
CfoI	GCGC	1 cut(s)	421
Cfr13I	GGNCC	2 cut(s)	210, 232
ClaI	ATCGAT	1 cut(s)	1032
CseI	GACGC	1 cut(s)	676
Csp6I	GTAC	2 cut(s)	1192, 1200
CviAII	CATG	9 cut(s)	121, 280, 406, 512, 517, 706, 932, 940, 1222
CviQI	GTAC	2 cut(s)	1192, 1200
DdeI	CTNAG	2 cut(s)	249, 945
EciI	GGCGGA	1 cut(s)	184
Eco24I	GRGCYC	1 cut(s)	1024
Eco31I	GGTCTC	1 cut(s)	493
Eco32I	GATATC	2 cut(s)	457, 1165
Eco57I	CTGAAG	1 cut(s)	95
Eco88I	CYCGRG	1 cut(s)	1023
EcoRII	CCWGG	2 cut(s)	499, 726
EcoRV	GATATC	2 cut(s)	457, 1165
EcoT22I	ATGCAT	1 cut(s)	933
EcoT38I	GRGCYC	1 cut(s)	1024
FaeI	CATG	9 cut(s)	124, 283, 409, 515, 520, 709, 935, 943, 1225
FatI	CATG	9 cut(s)	120, 279, 405, 511, 516, 705, 931, 939, 1221
FblI	GTMKAC	3 cut(s)	309, 535, 621
Fnu4HI	GCNGC	5 cut(s)	6, 61, 64, 235, 366
FokI	GGATG	2 cut(s)	116, 185
FriOI	GRGCYC	1 cut(s)	1024
Fsp4HI	GCNGC	5 cut(s)	6, 61, 64, 235, 366
FspBI	CTAG	3 cut(s)	290, 467, 906
GlaI	GCGC	1 cut(s)	420
GluI	GCNGC	5 cut(s)	6, 61, 64, 235, 366
GsuI	CTGGAG	2 cut(s)	107, 304
HaeIII	GGCC	2 cut(s)	212, 234
HgaI	GACGC	1 cut(s)	676
HhaI	GCGC	1 cut(s)	421
Hin1I	GRCGYC	1 cut(s)	240
Hin1II	CATG	9 cut(s)	124, 283, 409, 515, 520, 709, 935, 943, 1225
Hin6I	GCGC	1 cut(s)	419
HinP1I	GCGC	1 cut(s)	419
HincII	GTYRAC	1 cut(s)	310
HindII	GTYRAC	1 cut(s)	310
HinfI	GANTC	5 cut(s)	507, 520, 572, 759, 996
HphI	GGTGA	1 cut(s)	461
Hpy166II	GTNNAC	4 cut(s)	310, 536, 622, 880
Hpy8I	GTNNAC	4 cut(s)	310, 536, 622, 880
Hpy99I	CGWCG	4 cut(s)	103, 242, 311, 670
HpyAV	CCTTC	3 cut(s)	70, 943, 964
HpyCH4III	ACNGT	3 cut(s)	307, 1191, 1199
HpyCH4IV	ACGT	2 cut(s)	240, 736
HpyCH4V	TGCA	5 cut(s)	429, 779, 861, 926, 931
HpyF10VI	GCNNNNNNNGC	4 cut(s)	11, 435, 923, 1048
HpyF3I	CTNAG	2 cut(s)	249, 945
HpySE526I	ACGT	2 cut(s)	240, 736
Hsp92I	GRCGYC	1 cut(s)	240
Hsp92II	CATG	9 cut(s)	124, 283, 409, 515, 520, 709, 935, 943, 1225
HspAI	GCGC	1 cut(s)	419
LmnI	GCTCC	1 cut(s)	675
Lsp1109I	GCAGC	2 cut(s)	17, 75
LweI	GCATC	5 cut(s)	174, 888, 949, 1038, 1206
MaeI	CTAG	3 cut(s)	290, 467, 906
MaeII	ACGT	2 cut(s)	240, 736
MaeIII	GTNAC	2 cut(s)	737, 908
MboII	GAAGA	5 cut(s)	616, 797, 800, 803, 1061
MflI	RGATCY	2 cut(s)	723, 1170
MhlI	GDGCHC	1 cut(s)	1024
MluCI	AATT	4 cut(s)	561, 609, 681, 767
MlyI	GAGTC	2 cut(s)	516, 1005
MmeI	TCCRAC	2 cut(s)	81, 374
Mph1103I	ATGCAT	1 cut(s)	933
MseI	TTAA	1 cut(s)	732
MslI	CAYNNNNRTG	1 cut(s)	1226
MspR9I	CCNGG	2 cut(s)	501, 728
Mva1269I	GAATGC	1 cut(s)	421
MvaI	CCWGG	2 cut(s)	501, 728
MvnI	CGCG	3 cut(s)	20, 237, 665
MwoI	GCNNNNNNNGC	4 cut(s)	11, 435, 923, 1048
NlaIII	CATG	9 cut(s)	124, 283, 409, 515, 520, 709, 935, 943, 1225
NlaIV	GGNNCC	2 cut(s)	36, 725
NmuCI	GTSAC	1 cut(s)	737
NsiI	ATGCAT	1 cut(s)	933
NspI	RCATGY	1 cut(s)	943
PagI	TCATGA	2 cut(s)	120, 705
PcsI	WCGNNNNNNNCGW	3 cut(s)	234, 1114, 1123
PctI	GAATGC	1 cut(s)	421
PfeI	GAWTC	3 cut(s)	520, 572, 759
PkrI	GCNGC	5 cut(s)	7, 62, 65, 236, 367
Ple19I	CGATCG	1 cut(s)	50
PleI	GAGTC	2 cut(s)	515, 1004
PpsI	GAGTC	2 cut(s)	515, 1004
Psp6I	CCWGG	2 cut(s)	499, 726
PspGI	CCWGG	2 cut(s)	499, 726
PspN4I	GGNNCC	2 cut(s)	36, 725
PspPI	GGNCC	2 cut(s)	210, 232
PsuI	RGATCY	2 cut(s)	723, 1170
PvuI	CGATCG	1 cut(s)	50
RsaI	GTAC	2 cut(s)	1193, 1201
RsaNI	GTAC	2 cut(s)	1192, 1200
RseI	CAYNNNNRTG	1 cut(s)	1226
SalI	GTCGAC	1 cut(s)	308
SaqAI	TTAA	1 cut(s)	732
SatI	GCNGC	5 cut(s)	6, 61, 64, 235, 366
Sau96I	GGNCC	2 cut(s)	210, 232
ScaI	AGTACT	1 cut(s)	1193
SchI	GAGTC	2 cut(s)	516, 1005
ScrFI	CCNGG	2 cut(s)	501, 728
SduI	GDGCHC	1 cut(s)	1024
SfaNI	GCATC	5 cut(s)	174, 888, 949, 1038, 1206
SfcI	CTRYAG	2 cut(s)	622, 1195
SmiMI	CAYNNNNRTG	1 cut(s)	1226
SmlI	CTYRAG	1 cut(s)	143
SmoI	CTYRAG	1 cut(s)	143
SpeI	ACTAGT	1 cut(s)	905
Sse9I	AATT	4 cut(s)	561, 609, 681, 767
SsiI	CCGC	7 cut(s)	18, 60, 195, 235, 365, 663, 901
SspMI	CTAG	3 cut(s)	290, 467, 906
StyD4I	CCNGG	2 cut(s)	499, 726
TaaI	ACNGT	3 cut(s)	307, 1191, 1199
TaiI	ACGT	2 cut(s)	243, 739
TaqI	TCGA	8 cut(s)	129, 183, 228, 309, 544, 1032, 1081, 1117
TaqII	GACCGA	1 cut(s)	725
TasI	AATT	4 cut(s)	561, 609, 681, 767
TatI	WGTACW	1 cut(s)	1191
TauI	GCSGC	3 cut(s)	63, 237, 368
TfiI	GAWTC	3 cut(s)	520, 572, 759
Tru1I	TTAA	1 cut(s)	732
Tru9I	TTAA	1 cut(s)	732
TscAI	CASTG	1 cut(s)	271
TseFI	GTSAC	1 cut(s)	737
TseI	GCWGC	2 cut(s)	5, 63
Tsp45I	GTSAC	1 cut(s)	737
TspDTI	ATGAA	8 cut(s)	56, 152, 585, 693, 694, 983, 1085, 1238
TspGWI	ACGGA	1 cut(s)	489
TspRI	CASTG	1 cut(s)	271
XapI	RAATTY	2 cut(s)	609, 681
XceI	RCATGY	1 cut(s)	943
XmiI	GTMKAC	3 cut(s)	309, 535, 621
XspI	CTAG	3 cut(s)	290, 467, 906
ZraI	GACGTC	1 cut(s)	241
ZrmI	AGTACT	1 cut(s)	1193
Zsp2I	ATGCAT	1 cut(s)	933

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.