MD10G1219800.v1.1

ABC transporter D family member 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001312
Physical Location & Seq
Forward (+)
31767666 .. 31768304
639 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1219800.v1.1.491

Sequence Viewer

Length: 498 bp
ATGGAAAAAATGATTATACAGGTACAACAAACATACTCTGTCTCACTCTGCTCCTCCTCCGCCTATAACTCCGCCAACCGCCGCCGTTGCCGCCAAATTGGAGTGAGAGTGAGCGACGGTGTCAAAAGAGGAGCAACGATATCGTATGCTAATTGGCTCCTCTTCCCCAGATTTCCATCATTGTCCACCGTGTCCACCACTAGCAGAAGCAGCAGTAACTGGATGGTGAGGAGGAGAGAGAGCAGCAACGACGTCGAATCATCACCTTCTTCTTCGAGATCTTCTGGGCGCTGTGTAGCCGCCTCGGGTTCTGCACCGCCGCCGCCAGTTCCGCCAGCTACAGAAAAGCAGAGGGAGGTGCCTGAACTGCAGACGCTTCTCCGAAGATTCTGGAAGGTGGCGGCTCCCTATTGGTCCTCATCCGACAAGGTGCAAGCGAGGATCCTGATAGAGATAGGTTGTGTGGCTATGATTTTACCACTTGTATCAGTTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC_membrane_2 ABC_tran

Protein Analysis

166

Amino Acids

18.2

Weight (kDa)

10.46

Isoelectric Point (pI)

86.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 255
AccB1I GGYRCC 1 cut(s) 358
AclWI GGATC 2 cut(s) 436, 449
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 1 cut(s) 24
AluBI AGCT 1 cut(s) 338
AluI AGCT 1 cut(s) 338
Alw26I GTCTC 1 cut(s) 46
AlwI GGATC 2 cut(s) 436, 449
AlwNI CAGNNNCTG 1 cut(s) 219
Ama87I CYCGRG 1 cut(s) 304
ApeKI GCWGC 2 cut(s) 210, 243
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 1 cut(s) 414
AsuHPI GGTGA 2 cut(s) 238, 255
AvaI CYCGRG 1 cut(s) 304
AvaII GGWCC 1 cut(s) 414
BamHI GGATCC 1 cut(s) 441
BanI GGYRCC 1 cut(s) 358
BbvI GCAGC 2 cut(s) 222, 255
BccI CCATC 2 cut(s) 184, 217
BceAI ACGGC 1 cut(s) 69
BcgI CGANNNNNNTGC 4 cut(s) 123, 157, 235, 269
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 1 cut(s) 201
BfmI CTRYAG 2 cut(s) 339, 368
BfoI RGCGCY 1 cut(s) 292
BglII AGATCT 1 cut(s) 278
BisI GCNGC 8 cut(s) 82, 91, 211, 244, 300, 320, 323, 402
BlsI GCNGC 8 cut(s) 83, 92, 212, 245, 301, 321, 324, 403
Bme18I GGWCC 1 cut(s) 414
BmeT110I CYCGRG 1 cut(s) 304
BmgT120I GGNCC 1 cut(s) 414
BmiI GGNNCC 4 cut(s) 158, 360, 405, 443
BsaBI GATNNNNATC 1 cut(s) 175
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 303
BsaXI ACNNNNNCTCC 2 cut(s) 93, 123
Bse1I ACTGG 2 cut(s) 224, 326
Bse8I GATNNNNATC 1 cut(s) 175
BseDI CCNNGG 1 cut(s) 303
BseGI GGATG 2 cut(s) 228, 419
BseJI GATNNNNATC 1 cut(s) 175
BseNI ACTGG 2 cut(s) 224, 326
BseRI GAGGAG 6 cut(s) 43, 46, 144, 149, 244, 247
BseXI GCAGC 2 cut(s) 222, 255
BsgI GTGCAG 1 cut(s) 297
BshNI GGYRCC 1 cut(s) 358
BsiHKCI CYCGRG 1 cut(s) 304
BsmAI GTCTC 1 cut(s) 46
BsoBI CYCGRG 1 cut(s) 304
Bsp143I GATC 2 cut(s) 278, 441
BspLI GGNNCC 4 cut(s) 158, 360, 405, 443
BspMAI CTGCAG 1 cut(s) 372
BspPI GGATC 2 cut(s) 436, 449
BspT107I GGYRCC 1 cut(s) 358
BsrI ACTGG 2 cut(s) 224, 326
BssECI CCNNGG 1 cut(s) 303
BssMI GATC 2 cut(s) 278, 441
BssNI GRCGYC 1 cut(s) 252
Bst4CI ACNGT 2 cut(s) 119, 190
Bst6I CTCTTC 1 cut(s) 167
BstACI GRCGYC 1 cut(s) 252
BstC8I GCNNGC 2 cut(s) 336, 435
BstF5I GGATG 2 cut(s) 228, 419
BstH2I RGCGCY 1 cut(s) 292
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 2 cut(s) 281, 444
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 2 cut(s) 278, 441
BstMWI GCNNNNNNNGC 5 cut(s) 87, 90, 210, 331, 367
BstSFI CTRYAG 2 cut(s) 339, 368
BstV1I GCAGC 2 cut(s) 222, 255
BstX2I RGATCY 2 cut(s) 278, 441
BstYI RGATCY 2 cut(s) 278, 441
BtsCI GGATG 2 cut(s) 228, 419
Cac8I GCNNGC 2 cut(s) 336, 435
CaiI CAGNNNCTG 1 cut(s) 219
CfoI GCGC 1 cut(s) 291
Cfr13I GGNCC 1 cut(s) 414
CseI GACGC 1 cut(s) 382
Csp6I GTAC 1 cut(s) 23
CviJI RGCY 5 cut(s) 157, 299, 338, 404, 467
CviKI_1 RGCY 5 cut(s) 157, 299, 338, 404, 467
CviQI GTAC 1 cut(s) 23
DpnI GATC 2 cut(s) 280, 443
DpnII GATC 2 cut(s) 278, 441
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
EciI GGCGGA 3 cut(s) 49, 61, 321
Eco32I GATATC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 414
Eco88I CYCGRG 1 cut(s) 304
EcoRV GATATC 1 cut(s) 141
FaiI YATR 5 cut(s) 17, 34, 66, 147, 470
Fnu4HI GCNGC 8 cut(s) 82, 91, 211, 244, 300, 320, 323, 402
FokI GGATG 2 cut(s) 235, 406
Fsp4HI GCNGC 8 cut(s) 82, 91, 211, 244, 300, 320, 323, 402
FspBI CTAG 1 cut(s) 201
GlaI GCGC 1 cut(s) 290
GluI GCNGC 8 cut(s) 82, 91, 211, 244, 300, 320, 323, 402
HaeII RGCGCY 1 cut(s) 292
HgaI GACGC 1 cut(s) 382
HhaI GCGC 1 cut(s) 291
Hin1I GRCGYC 1 cut(s) 252
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HinfI GANTC 2 cut(s) 257, 387
HphI GGTGA 2 cut(s) 238, 255
Hpy166II GTNNAC 2 cut(s) 186, 195
Hpy188I TCNGA 2 cut(s) 383, 424
Hpy188III TCNNGA 3 cut(s) 276, 391, 445
Hpy8I GTNNAC 2 cut(s) 186, 195
Hpy99I CGWCG 3 cut(s) 119, 254, 257
HpyAV CCTTC 2 cut(s) 276, 388
HpyCH4III ACNGT 2 cut(s) 119, 190
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 3 cut(s) 314, 370, 433
HpyF10VI GCNNNNNNNGC 5 cut(s) 87, 90, 210, 331, 367
HpySE526I ACGT 1 cut(s) 252
Hsp92I GRCGYC 1 cut(s) 252
HspAI GCGC 1 cut(s) 289
Kzo9I GATC 2 cut(s) 278, 441
LmnI GCTCC 4 cut(s) 56, 131, 162, 409
LpnPI CCDG 9 cut(s) 5, 181, 205, 270, 339, 348, 375, 376, 458
Lsp1109I GCAGC 2 cut(s) 222, 255
MaeI CTAG 1 cut(s) 201
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 1 cut(s) 215
MalI GATC 2 cut(s) 280, 443
MboI GATC 2 cut(s) 278, 441
MboII GAAGA 5 cut(s) 154, 261, 264, 273, 396
MflI RGATCY 2 cut(s) 278, 441
MluCI AATT 2 cut(s) 96, 151
MmeI TCCRAC 1 cut(s) 447
MwoI GCNNNNNNNGC 5 cut(s) 87, 90, 210, 331, 367
NdeII GATC 2 cut(s) 278, 441
NlaIV GGNNCC 4 cut(s) 158, 360, 405, 443
PfeI GAWTC 2 cut(s) 257, 387
PflFI GACNNNGTC 1 cut(s) 119
PkrI GCNGC 8 cut(s) 83, 92, 212, 245, 301, 321, 324, 403
PspN4I GGNNCC 4 cut(s) 158, 360, 405, 443
PspPI GGNCC 1 cut(s) 414
PstI CTGCAG 1 cut(s) 372
PstNI CAGNNNCTG 1 cut(s) 219
PsuI RGATCY 2 cut(s) 278, 441
PsyI GACNNNGTC 1 cut(s) 119
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
SatI GCNGC 8 cut(s) 82, 91, 211, 244, 300, 320, 323, 402
Sau3AI GATC 2 cut(s) 278, 441
Sau96I GGNCC 1 cut(s) 414
SetI ASST 8 cut(s) 24, 255, 268, 340, 360, 399, 432, 460
SfcI CTRYAG 2 cut(s) 339, 368
SinI GGWCC 1 cut(s) 414
Sse9I AATT 2 cut(s) 96, 151
SspMI CTAG 1 cut(s) 201
TaaI ACNGT 2 cut(s) 119, 190
TaiI ACGT 1 cut(s) 255
TaqI TCGA 2 cut(s) 255, 275
TasI AATT 2 cut(s) 96, 151
TauI GCSGC 6 cut(s) 84, 93, 302, 322, 325, 404
TfiI GAWTC 2 cut(s) 257, 387
TseI GCWGC 2 cut(s) 210, 243
Tth111I GACNNNGTC 1 cut(s) 119
VpaK11BI GGWCC 1 cut(s) 414
XspI CTAG 1 cut(s) 201
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.