MD10G1220400.v1.1

ABC transporter D family member 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001312
Physical Location & Seq
Forward (+)
31858838 .. 31863419
4582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1220400.v1.1.491

Sequence Viewer

Length: 1815 bp
ATGATAATTGAAGACAAGAACCAAAAACAATTCACGAAGCAGCTGCTTTACTACTTAGCTGCATTTGCTGGTGGAATTCCGATTTTTGTGTTGCGATATTATGCAAGACAAACTCTTTCGTTGAGGTGGAGAGCTTGGATGACAAAACATTACATGGACCGTTACCTAAGCAACCAAGCCTTTTATAAAATTCAATCCCAATCAATGATTGATAATCCAGATCAGCGCATTGTTGATGATCTAAATTCGTTCACTGGGACTGCCCTATCCTTTACTGTAACAATTTTCGATGCTGTGATAGATCTCATCTCGTTCAGTAACATCTTATTCAGTATCTATCCTCCGCTGTTTGCTGTTCTATTTGTATATTCAATTGGTGGAACAGCCATAAGTATCTATCTTGGAAAGAATTTGGTTTCTTTAAACTTCATGCAAGAGAAAAAGGAAGCTGATTTTCGTTATGGACTTGTGCGCGTCAGAGAAAATGCTGAATCCATTGCTTTCTATAGTGGTGAAGCAAATGAAATGCAACTTTTGCTACAACGCTTCACAAGTGCTTTCAAAAATATGAGTAAATTGTTGATATGTTCAAGAAATGTCGACTTTTTCACCAGTTGGTACATCTATCTTGTTCAGATTCTTCCCGCTGCTGTTGTTGCCCCTATGTATTTCTCAGGCAAAATTGGGTTTGGTGTCATTAATCAGTCCACATCTGCTTTCAATCATATTCTTGATGACTTCTCCCTTATTGTCTACGAAATTCAGGCTATCAGTGCATTTGCAGCTGTAATTAATCGACTTGGTGAATTTGACGATCTATTGGATAGGAGCAAGTCGGAACGCCATTCTGACCTCTCAGAAGGGATAAGTCTGATATATAGTAACGTCAAAAGTTTACCGGAACCGGAGTCAAATGGATCCATTCCCATAGAAAGGCAAGAAAAGTTAGTGGATATAGAGCATTTGACTGTGGAGACTCCAAGTGGTACTGCATTGGTTAGGAATCTATCGTTGGTAATCAACAAGAATGAGCATTTATTGGTAACAGGACCAAGTGGGAGCGGTAAAACATCTTTGTTAAGAGCTATGTGTGGTCTTTGGAGTACTGGAAAAGGAAAAATCATATTCCATGTGAAGGACGGAGAAGATAATCAACCGTCCATCTCTTCTGGTGTAGTAGTTCTTGACGTAGATACTGGTAGTGATAAATATGGGGAACTTGGAAGGCCCTCAAATAGGAATTATGAAGGCATATTTTTCCTTCCTCAAAAACCATATATGGTATTGGGAACACTTCGCCAACAGTTGCTTTATCCTACATGGGCTGAGGATGCGATTTCCACAGCGGACGGTACTAAACCAACTGGGTCACTTCCTTTCTTGAAGCAGGCACCAAAATCAGAACATACGAGTGAAAAGCCTAGTAAGCCCACAACGGAGGACCTGATACAGGTTTTGGAGGATGTCCGACTTGGCAATTTATTATCCCGATTCAGCAGTTTGGATACTTCAAATGATTGGTCTAGTGTTCTCTCCCTTGGAGAGCAGCAACGCCTTGCTTTTGCACGCTTACTGCTCTCCAAACCAAAATTGGTTCTACTGGATGAATCTACCAGTGCTTTAGATGAAGCCAACGAGGCTCATTTATATCAGCAGGTTGAAAAGGCGGGCATAACATATATAAGCGTTGGCCACCGGCGAACTCTGTACGACTTCCACAAGAAGAACTTACTGATATCCACCGTGGATCCCAGCAGCGCCGAACACAATTGGAGCATTGATTCCATCGATCGAGAAGCATCATGCAACTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC_membrane_2 ABC_tran

Protein Analysis

605

Amino Acids

67.77

Weight (kDa)

5.76

Isoelectric Point (pI)

35.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane_2 PF06472 4 - 224 9.5e-47 ABC transporter transmembrane region 2
ABC_membrane PF00664 4 - 242 5e-06 ABC transporter transmembrane region
SbmA_BacA PF05992 8 - 272 3.8e-18 SbmA/BacA-like family
ABC_tran PF00005 334 - 539 1.9e-18 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 186
Acc36I ACCTGC 1 cut(s) 1645
AccB1I GGYRCC 1 cut(s) 1390
AccBSI CCGCTC 1 cut(s) 1062
AccI GTMKAC 2 cut(s) 600, 753
AccII CGCG 1 cut(s) 474
AciI CCGC 5 cut(s) 344, 645, 1062, 1346, 1667
AclWI GGATC 4 cut(s) 912, 925, 1742, 1755
AcoI YGGCCR 1 cut(s) 1690
AcsI RAATTY 6 cut(s) 75, 189, 244, 409, 759, 806
AfaI GTAC 5 cut(s) 620, 988, 1105, 1354, 1709
AfiI CCNNNNNNNGG 4 cut(s) 933, 1135, 1236, 1450
AgsI TTSAA 9 cut(s) 11, 194, 372, 562, 591, 721, 1384, 1512, 1661
AjuI GAANNNNNNNTTGG 2 cut(s) 995, 1027
AluBI AGCT 6 cut(s) 43, 59, 134, 449, 785, 1085
AluI AGCT 6 cut(s) 43, 59, 134, 449, 785, 1085
Alw26I GTCTC 1 cut(s) 968
AlwI GGATC 4 cut(s) 912, 925, 1742, 1755
AoxI GGCC 2 cut(s) 1226, 1690
ApeKI GCWGC 7 cut(s) 40, 43, 59, 647, 782, 1546, 1755
ApoI RAATTY 6 cut(s) 75, 189, 244, 409, 759, 806
ArsI GACNNNNNNTTYG 2 cut(s) 1505, 1537
AseI ATTAAT 2 cut(s) 699, 792
AspLEI GCGC 3 cut(s) 228, 474, 1760
AspS9I GGNCC 4 cut(s) 157, 1049, 1227, 1441
AsuHPI GGTGA 3 cut(s) 524, 601, 815
AvaII GGWCC 3 cut(s) 157, 1049, 1441
BalI TGGCCA 1 cut(s) 1692
BamHI GGATCC 2 cut(s) 917, 1747
BanI GGYRCC 1 cut(s) 1390
BbsI GAAGAC 1 cut(s) 18
BbvCI CCTCAGC 1 cut(s) 1326
BbvI GCAGC 7 cut(s) 30, 46, 52, 634, 794, 1558, 1767
BccI CCATC 2 cut(s) 1169, 1793
BcgI CGANNNNNNTGC 2 cut(s) 25, 59
BciVI GTATCC 1 cut(s) 1498
BcoDI GTCTC 1 cut(s) 968
BfaI CTAG 2 cut(s) 1422, 1524
BfmI CTRYAG 1 cut(s) 505
BfoI RGCGCY 1 cut(s) 1761
BfuAI ACCTGC 1 cut(s) 1645
BfuI GTATCC 1 cut(s) 1498
BglI GCCNNNNNGGC 1 cut(s) 1637
BglII AGATCT 1 cut(s) 301
BisI GCNGC 7 cut(s) 41, 44, 60, 648, 783, 1547, 1756
BlsI GCNGC 7 cut(s) 42, 45, 61, 649, 784, 1548, 1757
BmcAI AGTACT 1 cut(s) 1105
Bme18I GGWCC 3 cut(s) 157, 1049, 1441
BmgT120I GGNCC 4 cut(s) 157, 1049, 1227, 1441
BmiI GGNNCC 4 cut(s) 903, 919, 1392, 1749
BmrI ACTGGG 2 cut(s) 264, 1374
BmsI GCATC 3 cut(s) 280, 1321, 1808
BmuI ACTGGG 2 cut(s) 264, 1374
BpiI GAAGAC 1 cut(s) 18
Bpu10I CCTNAGC 2 cut(s) 167, 1326
Bsa29I ATCGAT 1 cut(s) 1788
BsaJI CCNNGG 2 cut(s) 1537, 1743
BsaWI WCCGGW 2 cut(s) 898, 904
Bsc4I CCNNNNNNNGG 4 cut(s) 933, 1135, 1236, 1450
Bse118I RCCGGY 1 cut(s) 1695
Bse1I ACTGG 7 cut(s) 259, 612, 1111, 1201, 1369, 1605, 1614
Bse3DI GCAATG 1 cut(s) 495
BseCI ATCGAT 1 cut(s) 1788
BseDI CCNNGG 2 cut(s) 1537, 1743
BseGI GGATG 4 cut(s) 144, 1336, 1468, 1609
BseLI CCNNNNNNNGG 4 cut(s) 933, 1135, 1236, 1450
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 3 cut(s) 687, 870, 1317
BseNI ACTGG 7 cut(s) 259, 612, 1111, 1201, 1369, 1605, 1614
BseXI GCAGC 7 cut(s) 30, 46, 52, 634, 794, 1558, 1767
BseYI CCCAGC 1 cut(s) 1751
Bsh1236I CGCG 1 cut(s) 474
Bsh1285I CGRYCG 1 cut(s) 1792
BshFI GGCC 2 cut(s) 1228, 1692
BshNI GGYRCC 1 cut(s) 1390
BshVI ATCGAT 1 cut(s) 1788
BsiEI CGRYCG 1 cut(s) 1792
BsiSI CCGG 3 cut(s) 899, 905, 1696
BslFI GGGAC 1 cut(s) 271
BslI CCNNNNNNNGG 4 cut(s) 933, 1135, 1236, 1450
BsmAI GTCTC 1 cut(s) 968
BsmFI GGGAC 1 cut(s) 271
BsnI GGCC 2 cut(s) 1228, 1692
Bsp143I GATC 7 cut(s) 220, 238, 301, 814, 917, 1747, 1789
BspACI CCGC 5 cut(s) 344, 645, 1062, 1346, 1667
BspANI GGCC 2 cut(s) 1228, 1692
BspCNI CTCAG 3 cut(s) 686, 869, 1318
BspDI ATCGAT 1 cut(s) 1788
BspFNI CGCG 1 cut(s) 474
BspLI GGNNCC 4 cut(s) 903, 919, 1392, 1749
BspMI ACCTGC 1 cut(s) 1645
BspPI GGATC 4 cut(s) 912, 925, 1742, 1755
BspT107I GGYRCC 1 cut(s) 1390
BsrBI CCGCTC 1 cut(s) 1062
BsrDI GCAATG 1 cut(s) 495
BsrFI RCCGGY 1 cut(s) 1695
BsrI ACTGG 7 cut(s) 259, 612, 1111, 1201, 1369, 1605, 1614
BssAI RCCGGY 1 cut(s) 1695
BssECI CCNNGG 2 cut(s) 1537, 1743
BssMI GATC 7 cut(s) 220, 238, 301, 814, 917, 1747, 1789
BssT1I CCWWGG 1 cut(s) 1537
Bst4CI ACNGT 7 cut(s) 161, 277, 970, 1158, 1305, 1352, 1744
Bst6I CTCTTC 1 cut(s) 1171
BstAPI GCANNNNNTGC 1 cut(s) 535
BstC8I GCNNGC 3 cut(s) 1389, 1567, 1669
BstDEI CTNAG 5 cut(s) 55, 167, 673, 856, 1326
BstDSI CCRYGG 1 cut(s) 1743
BstENI CCTNNNNNAGG 2 cut(s) 1234, 1448
BstF5I GGATG 4 cut(s) 144, 1336, 1468, 1609
BstFNI CGCG 1 cut(s) 474
BstH2I RGCGCY 1 cut(s) 1761
BstHHI GCGC 3 cut(s) 228, 474, 1760
BstKTI GATC 7 cut(s) 223, 241, 304, 817, 920, 1750, 1792
BstMAI GTCTC 1 cut(s) 968
BstMBI GATC 7 cut(s) 220, 238, 301, 814, 917, 1747, 1789
BstMCI CGRYCG 1 cut(s) 1792
BstMWI GCNNNNNNNGC 8 cut(s) 65, 535, 656, 773, 782, 1331, 1426, 1637
BstSFI CTRYAG 1 cut(s) 505
BstUI CGCG 1 cut(s) 474
BstV1I GCAGC 7 cut(s) 30, 46, 52, 634, 794, 1558, 1767
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 3 cut(s) 301, 917, 1747
BstYI RGATCY 3 cut(s) 301, 917, 1747
Bsu15I ATCGAT 1 cut(s) 1788
BsuI GTATCC 1 cut(s) 1498
BsuRI GGCC 2 cut(s) 1228, 1692
BsuTUI ATCGAT 1 cut(s) 1788
BtgI CCRYGG 1 cut(s) 1743
BtsCI GGATG 4 cut(s) 144, 1336, 1468, 1609
BtsIMutI CAGTG 3 cut(s) 252, 778, 1621
BveI ACCTGC 1 cut(s) 1645
Cac8I GCNNGC 3 cut(s) 1389, 1567, 1669
CfoI GCGC 3 cut(s) 228, 474, 1760
Cfr10I RCCGGY 1 cut(s) 1695
Cfr13I GGNCC 4 cut(s) 157, 1049, 1227, 1441
ClaI ATCGAT 1 cut(s) 1788
CseI GACGC 1 cut(s) 463
Csp6I GTAC 5 cut(s) 619, 987, 1104, 1353, 1708
CviAII CATG 5 cut(s) 154, 430, 1130, 1320, 1803
CviQI GTAC 5 cut(s) 619, 987, 1104, 1353, 1708
DdeI CTNAG 5 cut(s) 55, 167, 673, 856, 1326
DpnI GATC 7 cut(s) 222, 240, 303, 816, 919, 1749, 1791
DpnII GATC 7 cut(s) 220, 238, 301, 814, 917, 1747, 1789
DraI TTTAAA 1 cut(s) 423
EaeI YGGCCR 1 cut(s) 1690
Eam1104I CTCTTC 1 cut(s) 1171
EarI CTCTTC 1 cut(s) 1171
Eco130I CCWWGG 1 cut(s) 1537
Eco32I GATATC 1 cut(s) 1737
Eco47I GGWCC 3 cut(s) 157, 1049, 1441
EcoNI CCTNNNNNAGG 2 cut(s) 1234, 1448
EcoO109I RGGNCCY 2 cut(s) 1227, 1441
EcoRI GAATTC 1 cut(s) 75
EcoRV GATATC 1 cut(s) 1737
EcoT14I CCWWGG 1 cut(s) 1537
ErhI CCWWGG 1 cut(s) 1537
FaeI CATG 5 cut(s) 157, 433, 1133, 1323, 1806
FalI AAGNNNNNCTT 2 cut(s) 1712, 1744
FaqI GGGAC 1 cut(s) 271
FatI CATG 5 cut(s) 153, 429, 1129, 1319, 1802
FauI CCCGC 2 cut(s) 652, 1660
FblI GTMKAC 2 cut(s) 600, 753
Fnu4HI GCNGC 7 cut(s) 41, 44, 60, 648, 783, 1547, 1756
FokI GGATG 4 cut(s) 151, 1343, 1475, 1616
Fsp4HI GCNGC 7 cut(s) 41, 44, 60, 648, 783, 1547, 1756
FspBI CTAG 2 cut(s) 1422, 1524
GlaI GCGC 3 cut(s) 227, 473, 1759
GluI GCNGC 7 cut(s) 41, 44, 60, 648, 783, 1547, 1756
GsaI CCCAGC 1 cut(s) 1755
HaeII RGCGCY 1 cut(s) 1761
HaeIII GGCC 2 cut(s) 1228, 1692
HapII CCGG 3 cut(s) 899, 905, 1696
HgaI GACGC 1 cut(s) 463
HhaI GCGC 3 cut(s) 228, 474, 1760
Hin1II CATG 5 cut(s) 157, 433, 1133, 1323, 1806
Hin6I GCGC 3 cut(s) 226, 472, 1758
HinP1I GCGC 3 cut(s) 226, 472, 1758
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HinfI GANTC 8 cut(s) 491, 637, 908, 976, 1003, 1491, 1607, 1781
HpaII CCGG 3 cut(s) 899, 905, 1696
HphI GGTGA 3 cut(s) 524, 601, 815
Hpy166II GTNNAC 5 cut(s) 252, 601, 708, 754, 896
Hpy188I TCNGA 9 cut(s) 81, 479, 636, 838, 850, 859, 873, 1402, 1469
Hpy188III TCNNGA 8 cut(s) 34, 218, 591, 731, 1184, 1381, 1488, 1793
Hpy8I GTNNAC 5 cut(s) 252, 601, 708, 754, 896
HpyAV CCTTC 5 cut(s) 854, 1129, 1218, 1241, 1271
HpyCH4III ACNGT 7 cut(s) 161, 277, 970, 1158, 1305, 1352, 1744
HpyCH4IV ACGT 2 cut(s) 885, 1188
HpyCH4V TGCA 9 cut(s) 62, 104, 433, 529, 776, 782, 992, 1565, 1806
HpyF10VI GCNNNNNNNGC 8 cut(s) 65, 535, 656, 773, 782, 1331, 1426, 1637
HpyF3I CTNAG 5 cut(s) 55, 167, 673, 856, 1326
HpySE526I ACGT 2 cut(s) 885, 1188
Hsp92II CATG 5 cut(s) 157, 433, 1133, 1323, 1806
HspAI GCGC 3 cut(s) 226, 472, 1758
Kzo9I GATC 7 cut(s) 220, 238, 301, 814, 917, 1747, 1789
LmnI GCTCC 3 cut(s) 828, 1059, 1773
Lsp1109I GCAGC 7 cut(s) 30, 46, 52, 634, 794, 1558, 1767
LweI GCATC 3 cut(s) 280, 1321, 1808
MaeI CTAG 2 cut(s) 1422, 1524
MaeII ACGT 2 cut(s) 885, 1188
MaeIII GTNAC 6 cut(s) 161, 277, 317, 881, 1042, 1368
MalI GATC 7 cut(s) 222, 240, 303, 816, 919, 1749, 1791
MbiI CCGCTC 1 cut(s) 1062
MboI GATC 7 cut(s) 220, 238, 301, 814, 917, 1747, 1789
MboII GAAGA 5 cut(s) 23, 632, 1157, 1158, 1735
MfeI CAATTG 2 cut(s) 372, 1768
MflI RGATCY 3 cut(s) 301, 917, 1747
MlsI TGGCCA 1 cut(s) 1692
MluNI TGGCCA 1 cut(s) 1692
MlyI GAGTC 2 cut(s) 917, 970
MmeI TCCRAC 2 cut(s) 816, 1492
MnlI CCTC 9 cut(s) 117, 351, 863, 1240, 1275, 1321, 1432, 1453, 1630
Mox20I TGGCCA 1 cut(s) 1692
MscI TGGCCA 1 cut(s) 1692
MseI TTAA 4 cut(s) 422, 699, 792, 1079
MslI CAYNNNNRTG 1 cut(s) 1410
Msp20I TGGCCA 1 cut(s) 1692
MspA1I CMGCKG 5 cut(s) 43, 346, 647, 785, 1346
MspI CCGG 3 cut(s) 899, 905, 1696
MunI CAATTG 2 cut(s) 372, 1768
MvnI CGCG 1 cut(s) 474
MwoI GCNNNNNNNGC 8 cut(s) 65, 535, 656, 773, 782, 1331, 1426, 1637
NdeII GATC 7 cut(s) 220, 238, 301, 814, 917, 1747, 1789
NlaIII CATG 5 cut(s) 157, 433, 1133, 1323, 1806
NlaIV GGNNCC 4 cut(s) 903, 919, 1392, 1749
NmuCI GTSAC 1 cut(s) 1368
PfeI GAWTC 6 cut(s) 491, 637, 1003, 1491, 1607, 1781
PkrI GCNGC 7 cut(s) 42, 45, 61, 649, 784, 1548, 1757
Ple19I CGATCG 1 cut(s) 1792
PleI GAGTC 2 cut(s) 916, 970
PpsI GAGTC 2 cut(s) 916, 970
PpuMI RGGWCCY 1 cut(s) 1441
PshBI ATTAAT 2 cut(s) 699, 792
PsiI TTATAA 1 cut(s) 186
Psp5II RGGWCCY 1 cut(s) 1441
PspFI CCCAGC 1 cut(s) 1751
PspN4I GGNNCC 4 cut(s) 903, 919, 1392, 1749
PspPI GGNCC 4 cut(s) 157, 1049, 1227, 1441
PspPPI RGGWCCY 1 cut(s) 1441
PsuI RGATCY 3 cut(s) 301, 917, 1747
PvuI CGATCG 1 cut(s) 1792
PvuII CAGCTG 2 cut(s) 43, 785
RsaI GTAC 5 cut(s) 620, 988, 1105, 1354, 1709
RsaNI GTAC 5 cut(s) 619, 987, 1104, 1353, 1708
RseI CAYNNNNRTG 1 cut(s) 1410
SalI GTCGAC 1 cut(s) 599
SaqAI TTAA 4 cut(s) 422, 699, 792, 1079
SatI GCNGC 7 cut(s) 41, 44, 60, 648, 783, 1547, 1756
Sau3AI GATC 7 cut(s) 220, 238, 301, 814, 917, 1747, 1789
Sau96I GGNCC 4 cut(s) 157, 1049, 1227, 1441
ScaI AGTACT 1 cut(s) 1105
SchI GAGTC 2 cut(s) 917, 970
SfaNI GCATC 3 cut(s) 280, 1321, 1808
SfcI CTRYAG 1 cut(s) 505
SgrAI CRCCGGYG 1 cut(s) 1695
SinI GGWCC 3 cut(s) 157, 1049, 1441
SmiMI CAYNNNNRTG 1 cut(s) 1410
SsiI CCGC 5 cut(s) 344, 645, 1062, 1346, 1667
SspMI CTAG 2 cut(s) 1422, 1524
StyI CCWWGG 1 cut(s) 1537
TaaI ACNGT 7 cut(s) 161, 277, 970, 1158, 1305, 1352, 1744
TaiI ACGT 2 cut(s) 888, 1191
TaqI TCGA 5 cut(s) 288, 600, 796, 1788, 1792
TatI WGTACW 1 cut(s) 1103
TfiI GAWTC 6 cut(s) 491, 637, 1003, 1491, 1607, 1781
Tru1I TTAA 4 cut(s) 422, 699, 792, 1079
Tru9I TTAA 4 cut(s) 422, 699, 792, 1079
TscAI CASTG 3 cut(s) 259, 778, 1621
TseFI GTSAC 1 cut(s) 1368
TseI GCWGC 7 cut(s) 40, 43, 59, 647, 782, 1546, 1755
Tsp45I GTSAC 1 cut(s) 1368
TspDTI ATGAA 5 cut(s) 418, 537, 1260, 1620, 1641
TspGWI ACGGA 2 cut(s) 1155, 1451
TspRI CASTG 3 cut(s) 259, 778, 1621
VpaK11BI GGWCC 3 cut(s) 157, 1049, 1441
VspI ATTAAT 2 cut(s) 699, 792
XagI CCTNNNNNAGG 2 cut(s) 1234, 1448
XapI RAATTY 6 cut(s) 75, 189, 244, 409, 759, 806
XcmI CCANNNNNNNNNTGG 1 cut(s) 1588
XmiI GTMKAC 2 cut(s) 600, 753
XspI CTAG 2 cut(s) 1422, 1524
ZrmI AGTACT 1 cut(s) 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.