MD10G1220300.v1.1

ABC transporter D family member 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001312
Physical Location & Seq
Forward (+)
31844192 .. 31849828
5637 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1220300.v1.1.491

Sequence Viewer

Length: 2211 bp
ATGATGAGGAGGAGAGAGAGGAGCAAAGACGTCGACTCATCACCTGGGCGCTGTGCAGCCGCCTCGGGCTCTACAGTGCCGCCGCCAGTTCCGCCAGCTACAGAAAAGCTGGTCGCATTATTGCAGCAAAGGGAGGTGCCTAAACTGCGACCGCTTCTCCGAAGATTCTGGAAGGTGGCGGCTCCCTATTGGTCCTCCGATGACAAGGTACAAGCGAGGATAAGGCTCGCCGTAGTCTTCGCTCTCTCTCTCGCCACCACCGGTATCAGCGTCTGCTTCAATTTCCTCGGCCGTGACTTCTATAACGCCATTGCCGACAAGAACCAAAAACAATTCACGAAGCAGCTGCTTTACTACTTAGCTGCATTTGCCGGTGGAATTCCGATTTTTGTGTTGCGATATTATGCAAGACAAACTCTTTCTTTGAGGTGGAGAGCTTGGATGACAAAATATTACCTGGATCGTTACCTAAGCGACCAAACCTTTTATAAAATTCAATCCCAATCAATCATTGATAATCCAGATCAACGCATTGTTGATGATCTAAATTCGTTCACAGAGATTGCCCTATACCTCTCCGTAACACTTTTCAGTGCTGTGATAGATCTCATCTCGTTCAGTAACATCTTATTCAGTATCTATCCTCCGCTGTTTGTTGTTCTATTTGTATATTCAATTGGTGGAACAGTCATAAGTATCTTTCTTGGAAAGAATTTGGTTTCTTTAAACTTCATGCAAGAGAAAAAGGAAGCTGATTTTCGTTATGGACTTGTGCGCTGTTGTACCGACATTCCTAACATGCGCGTCAGAGAAAATGCTGAATCCATTGCTTTCTATAGTGGTGAAGCAAATGAAATGCAACTTTTGCTACAACGCTTCACAAGTGCTTTCATAAATATGAGCAAATTGTTGATATGTTCAAGAAATCTCGAGTTTTTCACTAGTGGATACAGCTATCTCATTCAGATTATTCCCGCTGCTGTTGTTGCTCCTATGTATTTCTCAGGCAAAATTGCGTTTGGTGTCATTAATCAGTCAGTATCTGCTTTCAGTCATATTCTTGCAGACTTCTCCCTTATTGTCTACCAATTTCAGACTATCAGTGCATTTGCAGCTGTAATTGATCGACTTGGTGAATTTGACGATTTGTTGGATAGCAGCAACCCGGAACGTCATTCTGACCCCTCGGAAGGGATAAGTCTGATATATAGTAACGTCAAAAGTTTACCGGAACCGGAGTCAAATGGATCCATTCCCATAGAAAAGCAACAAAAGTTAGTGGATATAGAGCACTTGACCGTGGAGACTCCAAGTGGTACTGCATTGGTTAGGAATTTATCGTTGGTAATCAACAAGAATGAGCATTTATTGGTAACAGGACCAAGTGGAAGCGGTAAAACATCTTTGTTAAGAGCTATGTGTGGTCTTTGGAGTACTGGAAAAGGAAAAATCATATTCTATGTGAAGGACGTTGAAGATAATCAACCGTCCATTTCTTCTGGTGTAGTAGTTCTTGAGATAGATACTGGTAGTGATAAATATGGGGAACTTGGAAGGCCCTCAAATAGGAATTATGAAGGCATATTTTTCCTTCCTCAAAAACCATATATGGTATTGGGAACACTTCGCCAACAGTTGCTTTATCCTACATGGGCTGAGGATGCGATTTCCACAACCGACGGTACTAAACCAACTGGTATGGACTTCAACTCCTTTTGCCACATGGTATGTATGCTGACTGCTGAGGAAGCTATAATTGTTGAGAGTTGTCCCACATCGATGATGAGGCACAAGGCACCAAAATCAGAACATACGAGTGAAAAGCCTAGTAAGCCCACAACGGAGGACCTGATACAGGTTTTGGAGGATGTCCGACTTGGCAATTTATTATCCCGATTCAGCAGTTTGGATACTTCAAATGACTGGTCTAGTGTTCTCTCCCTTGGAGAGCAGCAACGCCTTGCTTTTGTACGTTTACTGCTTTCGAAACCACAATTAGTTCTACTGGATGAATCTACCAGTGCTTTAGATGAAGCCAACGAGGTTCATTTATATCAGCAGATTGAAAAGGCAGGCATAACGTATATAAGCGTTGGCCACCGGCAAACTCTATACGACTTCCACAAGAAGAACTTACGGATCTCCACCGAGGAACCCAGCAGCGCCGAACCCAATTGGAGCATTGAATCCATCAATCGAGAAACAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC_membrane_2 ABC_tran

Protein Analysis

737

Amino Acids

82.7

Weight (kDa)

6.15

Isoelectric Point (pI)

41.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane_2 PF06472 70 - 334 9.6e-48 ABC transporter transmembrane region 2
SbmA_BacA PF05992 100 - 382 6.3e-17 SbmA/BacA-like family
ABC_tran PF00005 444 - 674 6.1e-17 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 489
AatII GACGTC 1 cut(s) 33
AccB1I GGYRCC 2 cut(s) 136, 1795
AccI GTMKAC 2 cut(s) 33, 1083
AccII CGCG 1 cut(s) 804
AciI CCGC 9 cut(s) 60, 80, 83, 92, 152, 179, 647, 975, 1392
AclWI GGATC 4 cut(s) 468, 1242, 1255, 2147
AcoI YGGCCR 2 cut(s) 289, 2095
AcsI RAATTY 6 cut(s) 378, 492, 547, 712, 1136, 1333
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 6 cut(s) 210, 784, 1318, 1435, 1684, 1971
AfiI CCNNNNNNNGG 4 cut(s) 1190, 1191, 1566, 1855
AgeI ACCGGT 1 cut(s) 260
AgsI TTSAA 9 cut(s) 280, 497, 675, 921, 1475, 1708, 1917, 2066, 2186
AhlI ACTAGT 1 cut(s) 941
AjnI CCWGG 2 cut(s) 43, 456
AjuI GAANNNNNNNTTGG 2 cut(s) 1325, 1357
Alw21I GWGCWC 1 cut(s) 1293
Alw26I GTCTC 1 cut(s) 1298
AlwI GGATC 4 cut(s) 468, 1242, 1255, 2147
AlwNI CAGNNNCTG 2 cut(s) 273, 1043
Ama87I CYCGRG 2 cut(s) 64, 929
AoxI GGCC 3 cut(s) 289, 1556, 2095
ApoI RAATTY 6 cut(s) 378, 492, 547, 712, 1136, 1333
ArsI GACNNNNNNTTYG 2 cut(s) 1910, 1942
AseI ATTAAT 1 cut(s) 1029
AsiGI ACCGGT 1 cut(s) 260
AspLEI GCGC 4 cut(s) 51, 777, 804, 2165
AspS9I GGNCC 4 cut(s) 192, 1379, 1557, 1846
AsuC2I CCSGG 1 cut(s) 1166
AsuHPI GGTGA 3 cut(s) 33, 854, 1145
AsuII TTCGAA 1 cut(s) 1985
AvaI CYCGRG 2 cut(s) 64, 929
AvaII GGWCC 3 cut(s) 192, 1379, 1846
BalI TGGCCA 1 cut(s) 2097
BamHI GGATCC 1 cut(s) 1247
BanI GGYRCC 2 cut(s) 136, 1795
BanII GRGCYC 1 cut(s) 71
BbsI GAAGAC 1 cut(s) 229
Bbv12I GWGCWC 1 cut(s) 1293
BbvCI CCTCAGC 2 cut(s) 1656, 1743
BccI CCATC 1 cut(s) 2198
BceAI ACGGC 2 cut(s) 215, 276
BcgI CGANNNNNNTGC 6 cut(s) 13, 45, 47, 79, 328, 362
BciT130I CCWGG 2 cut(s) 45, 458
BciVI GTATCC 2 cut(s) 941, 1903
BcnI CCSGG 1 cut(s) 1166
BcoDI GTCTC 1 cut(s) 1298
BcuI ACTAGT 1 cut(s) 941
BfaI CTAG 3 cut(s) 942, 1827, 1929
BfmI CTRYAG 3 cut(s) 72, 99, 835
BfoI RGCGCY 2 cut(s) 52, 2166
BfuI GTATCC 2 cut(s) 941, 1903
BglII AGATCT 1 cut(s) 604
BmcAI AGTACT 1 cut(s) 1435
Bme1390I CCNGG 3 cut(s) 45, 458, 1166
Bme18I GGWCC 3 cut(s) 192, 1379, 1846
BmeT110I CYCGRG 2 cut(s) 64, 929
BmgT120I GGNCC 4 cut(s) 192, 1379, 1557, 1846
BmiI GGNNCC 6 cut(s) 138, 183, 1233, 1249, 1797, 2154
BmrFI CCNGG 3 cut(s) 45, 458, 1166
BmsI GCATC 1 cut(s) 1651
BpiI GAAGAC 1 cut(s) 229
Bpu10I CCTNAGC 3 cut(s) 470, 1656, 1743
Bpu14I TTCGAA 1 cut(s) 1985
BpuEI CTTGAG 1 cut(s) 1535
BpuMI CCSGG 1 cut(s) 1166
Bsa29I ATCGAT 1 cut(s) 1778
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 7 cut(s) 44, 63, 286, 1185, 1299, 1942, 2148
BsaWI WCCGGW 3 cut(s) 260, 1228, 1234
BsaXI ACNNNNNCTCC 4 cut(s) 141, 171, 1694, 1724
Bsc4I CCNNNNNNNGG 4 cut(s) 1190, 1191, 1566, 1855
Bse118I RCCGGY 3 cut(s) 260, 371, 2100
Bse1I ACTGG 7 cut(s) 86, 1441, 1531, 1699, 1928, 2010, 2019
Bse3DI GCAATG 2 cut(s) 309, 825
BseBI CCWGG 2 cut(s) 45, 458
BseCI ATCGAT 1 cut(s) 1778
BseDI CCNNGG 7 cut(s) 44, 63, 286, 1185, 1299, 1942, 2148
BseGI GGATG 4 cut(s) 447, 1666, 1873, 2014
BseLI CCNNNNNNNGG 4 cut(s) 1190, 1191, 1566, 1855
BseMI GCAATG 2 cut(s) 309, 825
BseMII CTCAG 3 cut(s) 1017, 1647, 1734
BseNI ACTGG 7 cut(s) 86, 1441, 1531, 1699, 1928, 2010, 2019
BseRI GAGGAG 3 cut(s) 22, 25, 34
BseX3I CGGCCG 1 cut(s) 289
BseYI CCCAGC 1 cut(s) 2156
BsgI GTGCAG 1 cut(s) 75
Bsh1236I CGCG 1 cut(s) 804
Bsh1285I CGRYCG 2 cut(s) 152, 292
BshFI GGCC 3 cut(s) 291, 1558, 2097
BshNI GGYRCC 2 cut(s) 136, 1795
BshTI ACCGGT 1 cut(s) 260
BshVI ATCGAT 1 cut(s) 1778
BsiEI CGRYCG 2 cut(s) 152, 292
BsiHKAI GWGCWC 1 cut(s) 1293
BsiHKCI CYCGRG 2 cut(s) 64, 929
BsiSI CCGG 6 cut(s) 261, 372, 1166, 1229, 1235, 2101
BslFI GGGAC 1 cut(s) 1755
BslI CCNNNNNNNGG 4 cut(s) 1190, 1191, 1566, 1855
BsmAI GTCTC 1 cut(s) 1298
BsmFI GGGAC 1 cut(s) 1755
BsnI GGCC 3 cut(s) 291, 1558, 2097
BsoBI CYCGRG 2 cut(s) 64, 929
Bsp119I TTCGAA 1 cut(s) 1985
Bsp1286I GDGCHC 2 cut(s) 71, 1293
Bsp143I GATC 7 cut(s) 460, 523, 541, 604, 1123, 1247, 2139
BspACI CCGC 9 cut(s) 60, 80, 83, 92, 152, 179, 647, 975, 1392
BspANI GGCC 3 cut(s) 291, 1558, 2097
BspCNI CTCAG 3 cut(s) 1016, 1648, 1735
BspDI ATCGAT 1 cut(s) 1778
BspFNI CGCG 1 cut(s) 804
BspLI GGNNCC 6 cut(s) 138, 183, 1233, 1249, 1797, 2154
BspPI GGATC 4 cut(s) 468, 1242, 1255, 2147
BspT104I TTCGAA 1 cut(s) 1985
BspT107I GGYRCC 2 cut(s) 136, 1795
BsrDI GCAATG 2 cut(s) 309, 825
BsrFI RCCGGY 3 cut(s) 260, 371, 2100
BsrI ACTGG 7 cut(s) 86, 1441, 1531, 1699, 1928, 2010, 2019
BssAI RCCGGY 3 cut(s) 260, 371, 2100
BssECI CCNNGG 7 cut(s) 44, 63, 286, 1185, 1299, 1942, 2148
BssMI GATC 7 cut(s) 460, 523, 541, 604, 1123, 1247, 2139
BssNI GRCGYC 1 cut(s) 30
BssT1I CCWWGG 1 cut(s) 1942
Bst2UI CCWGG 2 cut(s) 45, 458
Bst4CI ACNGT 7 cut(s) 76, 688, 1300, 1488, 1635, 1682, 2206
BstACI GRCGYC 1 cut(s) 30
BstAPI GCANNNNNTGC 1 cut(s) 865
BstBI TTCGAA 1 cut(s) 1985
BstC8I GCNNGC 3 cut(s) 96, 228, 2074
BstDEI CTNAG 5 cut(s) 358, 470, 1003, 1656, 1743
BstDSI CCRYGG 1 cut(s) 1299
BstENI CCTNNNNNAGG 2 cut(s) 1564, 1853
BstF5I GGATG 4 cut(s) 447, 1666, 1873, 2014
BstFNI CGCG 1 cut(s) 804
BstH2I RGCGCY 2 cut(s) 52, 2166
BstHHI GCGC 4 cut(s) 51, 777, 804, 2165
BstKTI GATC 7 cut(s) 463, 526, 544, 607, 1126, 1250, 2142
BstMAI GTCTC 1 cut(s) 1298
BstMBI GATC 7 cut(s) 460, 523, 541, 604, 1123, 1247, 2139
BstMCI CGRYCG 2 cut(s) 152, 292
BstMWI GCNNNNNNNGC 9 cut(s) 91, 145, 368, 865, 986, 1112, 1661, 1748, 1831
BstNI CCWGG 2 cut(s) 45, 458
BstNSI RCATGY 1 cut(s) 802
BstSCI CCNGG 3 cut(s) 43, 456, 1164
BstSFI CTRYAG 3 cut(s) 72, 99, 835
BstUI CGCG 1 cut(s) 804
BstV2I GAAGAC 1 cut(s) 229
BstX2I RGATCY 3 cut(s) 604, 1247, 2139
BstYI RGATCY 3 cut(s) 604, 1247, 2139
BstZI CGGCCG 1 cut(s) 289
Bsu15I ATCGAT 1 cut(s) 1778
BsuI GTATCC 2 cut(s) 941, 1903
BsuRI GGCC 3 cut(s) 291, 1558, 2097
BsuTUI ATCGAT 1 cut(s) 1778
BtgI CCRYGG 1 cut(s) 1299
BtsCI GGATG 4 cut(s) 447, 1666, 1873, 2014
BtsIMutI CAGTG 4 cut(s) 81, 598, 1108, 2026
Cac8I GCNNGC 3 cut(s) 96, 228, 2074
CaiI CAGNNNCTG 2 cut(s) 273, 1043
CfoI GCGC 4 cut(s) 51, 777, 804, 2165
Cfr10I RCCGGY 3 cut(s) 260, 371, 2100
Cfr13I GGNCC 4 cut(s) 192, 1379, 1557, 1846
ClaI ATCGAT 1 cut(s) 1778
CseI GACGC 2 cut(s) 259, 793
Csp6I GTAC 6 cut(s) 209, 783, 1317, 1434, 1683, 1970
CspAI ACCGGT 1 cut(s) 260
CviAII CATG 4 cut(s) 733, 799, 1650, 1723
CviQI GTAC 6 cut(s) 209, 783, 1317, 1434, 1683, 1970
DdeI CTNAG 5 cut(s) 358, 470, 1003, 1656, 1743
DpnI GATC 7 cut(s) 462, 525, 543, 606, 1125, 1249, 2141
DpnII GATC 7 cut(s) 460, 523, 541, 604, 1123, 1247, 2139
DraI TTTAAA 1 cut(s) 726
EaeI YGGCCR 2 cut(s) 289, 2095
EagI CGGCCG 1 cut(s) 289
EciI GGCGGA 1 cut(s) 81
EclXI CGGCCG 1 cut(s) 289
Eco130I CCWWGG 1 cut(s) 1942
Eco24I GRGCYC 1 cut(s) 71
Eco47I GGWCC 3 cut(s) 192, 1379, 1846
Eco52I CGGCCG 1 cut(s) 289
Eco88I CYCGRG 2 cut(s) 64, 929
EcoNI CCTNNNNNAGG 2 cut(s) 1564, 1853
EcoO109I RGGNCCY 2 cut(s) 1557, 1846
EcoRI GAATTC 1 cut(s) 378
EcoRII CCWGG 2 cut(s) 43, 456
EcoT14I CCWWGG 1 cut(s) 1942
EcoT38I GRGCYC 1 cut(s) 71
ErhI CCWWGG 1 cut(s) 1942
FaeI CATG 4 cut(s) 736, 802, 1653, 1726
FalI AAGNNNNNCTT 2 cut(s) 2117, 2149
FaqI GGGAC 1 cut(s) 1755
FatI CATG 4 cut(s) 732, 798, 1649, 1722
FauI CCCGC 1 cut(s) 982
FblI GTMKAC 2 cut(s) 33, 1083
FokI GGATG 4 cut(s) 454, 1673, 1880, 2021
FriOI GRGCYC 1 cut(s) 71
FspBI CTAG 3 cut(s) 942, 1827, 1929
GlaI GCGC 4 cut(s) 50, 776, 803, 2164
GsaI CCCAGC 1 cut(s) 2160
HaeII RGCGCY 2 cut(s) 52, 2166
HaeIII GGCC 3 cut(s) 291, 1558, 2097
HapII CCGG 6 cut(s) 261, 372, 1166, 1229, 1235, 2101
HgaI GACGC 2 cut(s) 259, 793
HhaI GCGC 4 cut(s) 51, 777, 804, 2165
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 4 cut(s) 736, 802, 1653, 1726
Hin6I GCGC 4 cut(s) 49, 775, 802, 2163
HinP1I GCGC 4 cut(s) 49, 775, 802, 2163
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HinfI GANTC 8 cut(s) 35, 165, 821, 1238, 1306, 1896, 2012, 2186
HpaII CCGG 6 cut(s) 261, 372, 1166, 1229, 1235, 2101
HphI GGTGA 3 cut(s) 33, 854, 1145
Hpy166II GTNNAC 5 cut(s) 34, 555, 1084, 1226, 1976
Hpy188III TCNNGA 8 cut(s) 169, 337, 521, 921, 929, 1514, 1893, 2198
Hpy8I GTNNAC 5 cut(s) 34, 555, 1084, 1226, 1976
Hpy99I CGWCG 2 cut(s) 35, 1682
HpyAV CCTTC 6 cut(s) 166, 1184, 1459, 1548, 1571, 1601
HpyCH4III ACNGT 7 cut(s) 76, 688, 1300, 1488, 1635, 1682, 2206
HpyCH4IV ACGT 6 cut(s) 30, 1171, 1215, 1470, 1972, 2081
HpyF10VI GCNNNNNNNGC 9 cut(s) 91, 145, 368, 865, 986, 1112, 1661, 1748, 1831
HpyF3I CTNAG 5 cut(s) 358, 470, 1003, 1656, 1743
HpySE526I ACGT 6 cut(s) 30, 1171, 1215, 1470, 1972, 2081
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 4 cut(s) 736, 802, 1653, 1726
HspAI GCGC 4 cut(s) 49, 775, 802, 2163
Kzo9I GATC 7 cut(s) 460, 523, 541, 604, 1123, 1247, 2139
LmnI GCTCC 4 cut(s) 21, 187, 994, 2178
LweI GCATC 1 cut(s) 1651
MaeI CTAG 3 cut(s) 942, 1827, 1929
MaeII ACGT 6 cut(s) 30, 1171, 1215, 1470, 1972, 2081
MaeIII GTNAC 6 cut(s) 293, 464, 580, 620, 1211, 1372
MalI GATC 7 cut(s) 462, 525, 543, 606, 1125, 1249, 2141
MboI GATC 7 cut(s) 460, 523, 541, 604, 1123, 1247, 2139
MboII GAAGA 5 cut(s) 174, 229, 1487, 1488, 2140
MfeI CAATTG 2 cut(s) 675, 2173
MflI RGATCY 3 cut(s) 604, 1247, 2139
MhlI GDGCHC 2 cut(s) 71, 1293
MlsI TGGCCA 1 cut(s) 2097
MluNI TGGCCA 1 cut(s) 2097
MlyI GAGTC 3 cut(s) 29, 1247, 1300
MmeI TCCRAC 2 cut(s) 1131, 1897
Mox20I TGGCCA 1 cut(s) 2097
MscI TGGCCA 1 cut(s) 2097
MseI TTAA 3 cut(s) 725, 1029, 1409
MslI CAYNNNNRTG 3 cut(s) 896, 1778, 1815
Msp20I TGGCCA 1 cut(s) 2097
MspA1I CMGCKG 4 cut(s) 346, 649, 977, 1115
MspI CCGG 6 cut(s) 261, 372, 1166, 1229, 1235, 2101
MspR9I CCNGG 3 cut(s) 45, 458, 1166
MunI CAATTG 2 cut(s) 675, 2173
MvaI CCWGG 2 cut(s) 45, 458
MvnI CGCG 1 cut(s) 804
MwoI GCNNNNNNNGC 9 cut(s) 91, 145, 368, 865, 986, 1112, 1661, 1748, 1831
NciI CCSGG 1 cut(s) 1166
NdeII GATC 7 cut(s) 460, 523, 541, 604, 1123, 1247, 2139
NlaIII CATG 4 cut(s) 736, 802, 1653, 1726
NlaIV GGNNCC 6 cut(s) 138, 183, 1233, 1249, 1797, 2154
NmeAIII GCCGAG 1 cut(s) 267
NmuCI GTSAC 1 cut(s) 293
NspI RCATGY 1 cut(s) 802
NspV TTCGAA 1 cut(s) 1985
PaeR7I CTCGAG 1 cut(s) 929
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PfeI GAWTC 5 cut(s) 165, 821, 1896, 2012, 2186
PinAI ACCGGT 1 cut(s) 260
PleI GAGTC 3 cut(s) 29, 1246, 1300
PpsI GAGTC 3 cut(s) 29, 1246, 1300
PpuMI RGGWCCY 1 cut(s) 1846
PshBI ATTAAT 1 cut(s) 1029
PsiI TTATAA 1 cut(s) 489
Psp5II RGGWCCY 1 cut(s) 1846
Psp6I CCWGG 2 cut(s) 43, 456
PspFI CCCAGC 1 cut(s) 2156
PspGI CCWGG 2 cut(s) 43, 456
PspN4I GGNNCC 6 cut(s) 138, 183, 1233, 1249, 1797, 2154
PspPI GGNCC 4 cut(s) 192, 1379, 1557, 1846
PspPPI RGGWCCY 1 cut(s) 1846
PstNI CAGNNNCTG 2 cut(s) 273, 1043
PsuI RGATCY 3 cut(s) 604, 1247, 2139
PvuII CAGCTG 2 cut(s) 346, 1115
RsaI GTAC 6 cut(s) 210, 784, 1318, 1435, 1684, 1971
RsaNI GTAC 6 cut(s) 209, 783, 1317, 1434, 1683, 1970
RseI CAYNNNNRTG 3 cut(s) 896, 1778, 1815
SalI GTCGAC 1 cut(s) 32
SaqAI TTAA 3 cut(s) 725, 1029, 1409
Sau3AI GATC 7 cut(s) 460, 523, 541, 604, 1123, 1247, 2139
Sau96I GGNCC 4 cut(s) 192, 1379, 1557, 1846
ScaI AGTACT 1 cut(s) 1435
SchI GAGTC 3 cut(s) 29, 1247, 1300
ScrFI CCNGG 3 cut(s) 45, 458, 1166
SduI GDGCHC 2 cut(s) 71, 1293
SfaNI GCATC 1 cut(s) 1651
SfcI CTRYAG 3 cut(s) 72, 99, 835
Sfr274I CTCGAG 1 cut(s) 929
SfuI TTCGAA 1 cut(s) 1985
SinI GGWCC 3 cut(s) 192, 1379, 1846
SlaI CTCGAG 1 cut(s) 929
SmiMI CAYNNNNRTG 3 cut(s) 896, 1778, 1815
SmlI CTYRAG 2 cut(s) 929, 1514
SmoI CTYRAG 2 cut(s) 929, 1514
SpeI ACTAGT 1 cut(s) 941
SsiI CCGC 9 cut(s) 60, 80, 83, 92, 152, 179, 647, 975, 1392
SspI AATATT 1 cut(s) 452
SspMI CTAG 3 cut(s) 942, 1827, 1929
StyD4I CCNGG 3 cut(s) 43, 456, 1164
StyI CCWWGG 1 cut(s) 1942
TaaI ACNGT 7 cut(s) 76, 688, 1300, 1488, 1635, 1682, 2206
TaiI ACGT 6 cut(s) 33, 1174, 1218, 1473, 1975, 2084
TaqI TCGA 6 cut(s) 33, 930, 1126, 1778, 1985, 2197
TatI WGTACW 1 cut(s) 1433
TauI GCSGC 4 cut(s) 62, 82, 85, 182
TfiI GAWTC 5 cut(s) 165, 821, 1896, 2012, 2186
Tru1I TTAA 3 cut(s) 725, 1029, 1409
Tru9I TTAA 3 cut(s) 725, 1029, 1409
TscAI CASTG 4 cut(s) 81, 598, 1108, 2026
TseFI GTSAC 1 cut(s) 293
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 7 cut(s) 721, 867, 880, 1590, 2025, 2036, 2046
TspGWI ACGGA 3 cut(s) 568, 1856, 2152
TspRI CASTG 4 cut(s) 81, 598, 1108, 2026
VpaK11BI GGWCC 3 cut(s) 192, 1379, 1846
VspI ATTAAT 1 cut(s) 1029
XagI CCTNNNNNAGG 2 cut(s) 1564, 1853
XapI RAATTY 6 cut(s) 378, 492, 547, 712, 1136, 1333
XceI RCATGY 1 cut(s) 802
XhoI CTCGAG 1 cut(s) 929
XmiI GTMKAC 2 cut(s) 33, 1083
XspI CTAG 3 cut(s) 942, 1827, 1929
ZraI GACGTC 1 cut(s) 31
ZrmI AGTACT 1 cut(s) 1435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.