Rw6G012710

ABC transporter D family member 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001312
Physical Location & Seq
Forward (+)
23276265 .. 23284881
8617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G012710.1

Sequence Viewer

Length: 1596 bp
ATGAAGGATTGCTTAAAAGAGGAGTCCATCAAGGCTTCTACATTTGGACTTTTATTGCTAGCACATGGAAGACCACATGATTCTTCAGCGCCTCATTCAAGCTCTACACATGTTCTCCCCATCCAAGCAACGTTTGAGTTTGTTGATCATGCGGAGATGGTGATATGTGAGCACCTCAAATCTGATAAGGAGGTCATTCTTGCCGTGATTGTTAAACTTAAGAAAAGATGGAGACAATGGAAGAGGAGAAAGGAAAAGAGGAGCATCAAATCTCATTTTTCGACTGCTCCCAAGTTACCTAAGCAACTACGGGAGAGGAACAGTCCTTTCTCAAGAAGAAATTTCTGGCTTCTCTATCAATATGACCAACTATTTTTTTTTTTTAATGAATTTTCAAGCGGAAAAACAAAACTTGGTCATATTGATACTTTTCTTTCTGACATAATATGTATCCATATCTCTCATTGTTCATTTTCTTTAACACTCTTCAATGCTGTGATATATCTCATATCATTCAGTAACATCTTATTTGGTATCTATCCTCTGCGGTTTGTTGTTCTTCTAGTATATTCGATTGGTGGAACAGTGATCAGTGTCTTTATTGGAAGGCAATTGTTGATAGCTTCAAGAAATCTTGAGTTTTTCACCAGTGGATACTGCTATCTTATTCAAATTCTTCATGCTGCTGTCGTTGCTCCTATGTACCTCTCTAGAAAAATTGAGCTTGGTGTCATTAATCAGTCAGTATCGGCTTTTAATCATATCCTGGGGGACTTCTCCCTCACCGTGTACCAATTTCAGTCTATAAGCGCATTTTCAGCAGTAATTGATCGACTTGATGAATTTGATGATTATTTGGATATCAGCTCTCAACGTCATTCTGAACCTCCAGAAGGGATAAGTCTTACGTATAGTAATGTTAAAACTTTAGCTGAACTGGAGCCAAATGGATGCATACCCATAGATAAGCAACAAAAGCTACTGGACATAGAGAATTTGACTGTGCAAACACCAAGTAGCACTACATTGATTAGGGATTTATCATTGGTAATCAACAATAATGAGAATCTATGGGTGACAGGACCTAGCGGGAGTGGTAAAACTTATTTGTTAAGAATTTTGGCTGGTCTTTGGAGTGCTGGAAGAGGAAAGATCACATTCTATGTGGAAGATGAAGAAGAGCTTGTACCATCCAATTTTCCAGGTGTAGGTCCTCTTGGTACTGCTAATGATAAATATGGGGAACATGAAAGACCCTCAAATAGGAGTTATAAGGGCATATTTTTCGTTCCTAGAAGTTGTTACATAGAAGATTTGTATCTGGATATGACTGATGCATATTTTGCCATTGCTACCTTCAGATTGTGTTTTGCCCATGTGCTCTTGTTAATTAATTTAGATATACACAATATAGATTCTAATTCCTTTTGGAGAAGAAAGGAGATCGATGTGATTTTTTGGTCTCAGAGTAGCAGCACTAAGATTTCGGATAAAGGTACGTTTCATATCAAGAATGAAAATATAAGGCGAAAGAGGGTCTCCTTGTCTAATACCCCTGGAAGGGGCAAAGAAATCCTCTTGTTTTCCATTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC_membrane_2 ABC_tran

Protein Analysis

531

Amino Acids

60.63

Weight (kDa)

8.7

Isoelectric Point (pI)

50.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 345 - 388 1.6e-06 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1272
AciI CCGC 4 cut(s) 152, 399, 547, 1089
AclI AACGTT 1 cut(s) 131
AcsI RAATTY 6 cut(s) 340, 389, 672, 842, 994, 1116
AcuI CTGAAG 2 cut(s) 69, 1342
AfaI GTAC 5 cut(s) 704, 791, 1188, 1222, 1498
AfiI CCNNNNNNNGG 6 cut(s) 893, 1208, 1263, 1560, 1561, 1562
AflII CTTAAG 1 cut(s) 218
AflIII ACRYGT 1 cut(s) 109
AgsI TTSAA 5 cut(s) 99, 396, 490, 627, 671
AjnI CCWGG 3 cut(s) 765, 1201, 1555
AluBI AGCT 7 cut(s) 102, 623, 724, 867, 932, 979, 1183
AluI AGCT 7 cut(s) 102, 623, 724, 867, 932, 979, 1183
Alw21I GWGCWC 2 cut(s) 174, 1383
Alw26I GTCTC 3 cut(s) 226, 1467, 1543
ApeKI GCWGC 2 cut(s) 683, 1473
ApoI RAATTY 6 cut(s) 340, 389, 672, 842, 994, 1116
AseI ATTAAT 2 cut(s) 735, 1392
AspLEI GCGC 2 cut(s) 91, 812
AspS9I GGNCC 2 cut(s) 1082, 1211
AsuHPI GGTGA 4 cut(s) 172, 637, 775, 1087
AsuNHI GCTAGC 1 cut(s) 58
AvaII GGWCC 2 cut(s) 1082, 1211
BarI GAAGNNNNNNTAC 2 cut(s) 1170, 1202
BbsI GAAGAC 1 cut(s) 76
Bbv12I GWGCWC 2 cut(s) 174, 1383
BbvI GCAGC 2 cut(s) 670, 1485
BccI CCATC 5 cut(s) 35, 128, 151, 222, 1198
BceAI ACGGC 1 cut(s) 188
BcgI CGANNNNNNTGC 2 cut(s) 1267, 1301
BciT130I CCWGG 3 cut(s) 767, 1203, 1557
BciVI GTATCC 2 cut(s) 461, 647
BclI TGATCA 2 cut(s) 145, 588
BcoDI GTCTC 3 cut(s) 226, 1467, 1543
BfaI CTAG 5 cut(s) 59, 563, 711, 1086, 1293
BfoI RGCGCY 1 cut(s) 92
BfrI CTTAAG 1 cut(s) 218
BfuI GTATCC 2 cut(s) 461, 647
BisI GCNGC 2 cut(s) 684, 1474
BlsI GCNGC 2 cut(s) 685, 1475
Bme1390I CCNGG 3 cut(s) 767, 1203, 1557
Bme18I GGWCC 2 cut(s) 1082, 1211
BmgT120I GGNCC 2 cut(s) 1082, 1211
BmiI GGNNCC 1 cut(s) 942
BmrFI CCNGG 3 cut(s) 767, 1203, 1557
BmsI GCATC 3 cut(s) 273, 941, 1324
BmtI GCTAGC 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 76
BpmI CTGGAG 2 cut(s) 873, 959
Bpu10I CCTNAGC 1 cut(s) 300
BpuEI CTTGAG 2 cut(s) 316, 656
Bsa29I ATCGAT 1 cut(s) 1446
BsaAI YACGTR 1 cut(s) 909
BsaBI GATNNNNATC 1 cut(s) 1317
BsaI GGTCTC 2 cut(s) 1467, 1543
BsaJI CCNNGG 2 cut(s) 766, 1555
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 6 cut(s) 893, 1208, 1263, 1560, 1561, 1562
Bse1I ACTGG 3 cut(s) 648, 942, 987
Bse3DI GCAATG 1 cut(s) 1347
Bse8I GATNNNNATC 1 cut(s) 1317
BseBI CCWGG 3 cut(s) 767, 1203, 1557
BseCI ATCGAT 1 cut(s) 1446
BseDI CCNNGG 2 cut(s) 766, 1555
BseGI GGATG 3 cut(s) 120, 956, 1190
BseJI GATNNNNATC 1 cut(s) 1317
BseLI CCNNNNNNNGG 6 cut(s) 893, 1208, 1263, 1560, 1561, 1562
BseMI GCAATG 1 cut(s) 1347
BseMII CTCAG 1 cut(s) 1478
BseNI ACTGG 3 cut(s) 648, 942, 987
BseRI GAGGAG 3 cut(s) 35, 259, 274
BseXI GCAGC 2 cut(s) 670, 1485
BshVI ATCGAT 1 cut(s) 1446
BsiHKAI GWGCWC 2 cut(s) 174, 1383
BslFI GGGAC 1 cut(s) 785
BslI CCNNNNNNNGG 6 cut(s) 893, 1208, 1263, 1560, 1561, 1562
BsmAI GTCTC 3 cut(s) 226, 1467, 1543
BsmFI GGGAC 1 cut(s) 785
Bso31I GGTCTC 2 cut(s) 1467, 1543
Bsp1286I GDGCHC 2 cut(s) 174, 1383
Bsp143I GATC 5 cut(s) 145, 588, 829, 1152, 1443
BspACI CCGC 4 cut(s) 152, 399, 547, 1089
BspCNI CTCAG 1 cut(s) 1477
BspDI ATCGAT 1 cut(s) 1446
BspLI GGNNCC 1 cut(s) 942
BspOI GCTAGC 1 cut(s) 62
BspQI GCTCTTC 1 cut(s) 1173
BspTI CTTAAG 1 cut(s) 218
BspTNI GGTCTC 2 cut(s) 1467, 1543
BsrDI GCAATG 1 cut(s) 1347
BsrI ACTGG 3 cut(s) 648, 942, 987
BssECI CCNNGG 2 cut(s) 766, 1555
BssMI GATC 5 cut(s) 145, 588, 829, 1152, 1443
Bst2UI CCWGG 3 cut(s) 767, 1203, 1557
Bst4CI ACNGT 4 cut(s) 323, 586, 787, 1003
Bst6I CTCTTC 4 cut(s) 236, 491, 1138, 1173
BstAFI CTTAAG 1 cut(s) 218
BstAPI GCANNNNNTGC 1 cut(s) 1343
BstBAI YACGTR 1 cut(s) 909
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 3 cut(s) 300, 1464, 1479
BstENI CCTNNNNNAGG 2 cut(s) 891, 1261
BstF5I GGATG 3 cut(s) 120, 956, 1190
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 2 cut(s) 91, 812
BstKTI GATC 5 cut(s) 148, 591, 832, 1155, 1446
BstMAI GTCTC 3 cut(s) 226, 1467, 1543
BstMBI GATC 5 cut(s) 145, 588, 829, 1152, 1443
BstMWI GCNNNNNNNGC 4 cut(s) 692, 818, 976, 1343
BstNI CCWGG 3 cut(s) 767, 1203, 1557
BstNSI RCATGY 1 cut(s) 113
BstSCI CCNGG 3 cut(s) 765, 1201, 1555
BstSNI TACGTA 1 cut(s) 909
BstV1I GCAGC 2 cut(s) 670, 1485
BstV2I GAAGAC 1 cut(s) 76
Bsu15I ATCGAT 1 cut(s) 1446
BsuI GTATCC 2 cut(s) 461, 647
BsuTUI ATCGAT 1 cut(s) 1446
BtsCI GGATG 3 cut(s) 120, 956, 1190
BtsIMutI CAGTG 3 cut(s) 591, 598, 655
Cac8I GCNNGC 1 cut(s) 60
CfoI GCGC 2 cut(s) 91, 812
Cfr13I GGNCC 2 cut(s) 1082, 1211
ClaI ATCGAT 1 cut(s) 1446
Csp6I GTAC 5 cut(s) 703, 790, 1187, 1221, 1497
CviAII CATG 7 cut(s) 65, 77, 110, 149, 680, 1247, 1376
CviQI GTAC 5 cut(s) 703, 790, 1187, 1221, 1497
DdeI CTNAG 3 cut(s) 300, 1464, 1479
DpnI GATC 5 cut(s) 147, 590, 831, 1154, 1445
DpnII GATC 5 cut(s) 145, 588, 829, 1152, 1443
Eam1104I CTCTTC 4 cut(s) 236, 491, 1138, 1173
EarI CTCTTC 4 cut(s) 236, 491, 1138, 1173
Eco105I TACGTA 1 cut(s) 909
Eco31I GGTCTC 2 cut(s) 1467, 1543
Eco32I GATATC 1 cut(s) 862
Eco47I GGWCC 2 cut(s) 1082, 1211
Eco57I CTGAAG 2 cut(s) 69, 1342
EcoNI CCTNNNNNAGG 2 cut(s) 891, 1261
EcoO109I RGGNCCY 2 cut(s) 1082, 1211
EcoRII CCWGG 3 cut(s) 765, 1201, 1555
EcoRV GATATC 1 cut(s) 862
EcoT22I ATGCAT 2 cut(s) 956, 1339
FaeI CATG 7 cut(s) 68, 80, 113, 152, 683, 1250, 1379
FalI AAGNNNNNCTT 3 cut(s) 28, 1167, 1199
FaqI GGGAC 1 cut(s) 785
FatI CATG 7 cut(s) 64, 76, 109, 148, 679, 1246, 1375
FauI CCCGC 1 cut(s) 1082
FbaI TGATCA 2 cut(s) 145, 588
Fnu4HI GCNGC 2 cut(s) 684, 1474
FokI GGATG 3 cut(s) 107, 963, 1177
Fsp4HI GCNGC 2 cut(s) 684, 1474
FspBI CTAG 5 cut(s) 59, 563, 711, 1086, 1293
GlaI GCGC 2 cut(s) 90, 811
GluI GCNGC 2 cut(s) 684, 1474
GsuI CTGGAG 2 cut(s) 873, 959
HaeII RGCGCY 1 cut(s) 92
HhaI GCGC 2 cut(s) 91, 812
Hin1II CATG 7 cut(s) 68, 80, 113, 152, 683, 1250, 1379
Hin6I GCGC 2 cut(s) 89, 810
HinP1I GCGC 2 cut(s) 89, 810
HinfI GANTC 4 cut(s) 23, 80, 1066, 1415
HphI GGTGA 4 cut(s) 172, 637, 775, 1087
Hpy166II GTNNAC 1 cut(s) 790
Hpy188I TCNGA 6 cut(s) 184, 439, 883, 1361, 1467, 1489
Hpy188III TCNNGA 7 cut(s) 333, 627, 635, 711, 890, 1322, 1510
Hpy8I GTNNAC 1 cut(s) 790
HpyAV CCTTC 4 cut(s) 600, 887, 1366, 1554
HpyCH4III ACNGT 4 cut(s) 323, 586, 787, 1003
HpyCH4IV ACGT 4 cut(s) 131, 874, 908, 1499
HpyCH4V TGCA 3 cut(s) 954, 1006, 1337
HpyF10VI GCNNNNNNNGC 4 cut(s) 692, 818, 976, 1343
HpyF3I CTNAG 3 cut(s) 300, 1464, 1479
HpySE526I ACGT 4 cut(s) 131, 874, 908, 1499
Hsp92II CATG 7 cut(s) 68, 80, 113, 152, 683, 1250, 1379
HspAI GCGC 2 cut(s) 89, 810
Ksp22I TGATCA 2 cut(s) 145, 588
Kzo9I GATC 5 cut(s) 145, 588, 829, 1152, 1443
LguI GCTCTTC 1 cut(s) 1173
LmnI GCTCC 4 cut(s) 261, 292, 700, 940
Lsp1109I GCAGC 2 cut(s) 670, 1485
LweI GCATC 3 cut(s) 273, 941, 1324
MaeI CTAG 5 cut(s) 59, 563, 711, 1086, 1293
MaeII ACGT 4 cut(s) 131, 874, 908, 1499
MaeIII GTNAC 4 cut(s) 294, 518, 1075, 1301
MalI GATC 5 cut(s) 147, 590, 831, 1154, 1445
MboI GATC 5 cut(s) 145, 588, 829, 1152, 1443
MfeI CAATTG 1 cut(s) 611
MhlI GDGCHC 2 cut(s) 174, 1383
MlyI GAGTC 1 cut(s) 32
Mph1103I ATGCAT 2 cut(s) 956, 1339
MspCI CTTAAG 1 cut(s) 218
MspR9I CCNGG 3 cut(s) 767, 1203, 1557
MunI CAATTG 1 cut(s) 611
MvaI CCWGG 3 cut(s) 767, 1203, 1557
MwoI GCNNNNNNNGC 4 cut(s) 692, 818, 976, 1343
NdeII GATC 5 cut(s) 145, 588, 829, 1152, 1443
NheI GCTAGC 1 cut(s) 58
NlaIII CATG 7 cut(s) 68, 80, 113, 152, 683, 1250, 1379
NlaIV GGNNCC 1 cut(s) 942
NmuCI GTSAC 1 cut(s) 1075
NsiI ATGCAT 2 cut(s) 956, 1339
NspI RCATGY 1 cut(s) 113
PacI TTAATTAA 1 cut(s) 1392
PciI ACATGT 1 cut(s) 109
PciSI GCTCTTC 1 cut(s) 1173
PfeI GAWTC 3 cut(s) 80, 1066, 1415
PkrI GCNGC 2 cut(s) 685, 1475
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
Ppu21I YACGTR 1 cut(s) 909
PpuMI RGGWCCY 2 cut(s) 1082, 1211
PscI ACATGT 1 cut(s) 109
PshBI ATTAAT 2 cut(s) 735, 1392
PsiI TTATAA 1 cut(s) 1272
Psp1406I AACGTT 1 cut(s) 131
Psp5II RGGWCCY 2 cut(s) 1082, 1211
Psp6I CCWGG 3 cut(s) 765, 1201, 1555
PspGI CCWGG 3 cut(s) 765, 1201, 1555
PspN4I GGNNCC 1 cut(s) 942
PspPI GGNCC 2 cut(s) 1082, 1211
PspPPI RGGWCCY 2 cut(s) 1082, 1211
RsaI GTAC 5 cut(s) 704, 791, 1188, 1222, 1498
RsaNI GTAC 5 cut(s) 703, 790, 1187, 1221, 1497
SapI GCTCTTC 1 cut(s) 1173
SatI GCNGC 2 cut(s) 684, 1474
Sau3AI GATC 5 cut(s) 145, 588, 829, 1152, 1443
Sau96I GGNCC 2 cut(s) 1082, 1211
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 3 cut(s) 767, 1203, 1557
SduI GDGCHC 2 cut(s) 174, 1383
SfaNI GCATC 3 cut(s) 273, 941, 1324
SinI GGWCC 2 cut(s) 1082, 1211
SmlI CTYRAG 3 cut(s) 218, 331, 635
SmoI CTYRAG 3 cut(s) 218, 331, 635
SnaBI TACGTA 1 cut(s) 909
SsiI CCGC 4 cut(s) 152, 399, 547, 1089
SspMI CTAG 5 cut(s) 59, 563, 711, 1086, 1293
StyD4I CCNGG 3 cut(s) 765, 1201, 1555
TaaI ACNGT 4 cut(s) 323, 586, 787, 1003
TaiI ACGT 4 cut(s) 134, 877, 911, 1502
TaqI TCGA 4 cut(s) 281, 572, 832, 1446
TfiI GAWTC 3 cut(s) 80, 1066, 1415
TscAI CASTG 3 cut(s) 591, 598, 655
TseFI GTSAC 1 cut(s) 1075
TseI GCWGC 2 cut(s) 683, 1473
Tsp45I GTSAC 1 cut(s) 1075
TspDTI ATGAA 9 cut(s) 17, 402, 459, 668, 855, 1188, 1263, 1493, 1530
TspRI CASTG 3 cut(s) 591, 598, 655
Vha464I CTTAAG 1 cut(s) 218
VpaK11BI GGWCC 2 cut(s) 1082, 1211
VspI ATTAAT 2 cut(s) 735, 1392
XagI CCTNNNNNAGG 2 cut(s) 891, 1261
XapI RAATTY 6 cut(s) 340, 389, 672, 842, 994, 1116
XbaI TCTAGA 1 cut(s) 710
XceI RCATGY 1 cut(s) 113
XspI CTAG 5 cut(s) 59, 563, 711, 1086, 1293
Zsp2I ATGCAT 2 cut(s) 956, 1339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.