MD12G1048900.v1.1

Ribosomal protein S28e

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001317
Physical Location & Seq
Reverse (-)
5609265 .. 5609423
159 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1048900.v1.1.491

Sequence Viewer

Length: 159 bp
ATGGGACAGACTGGATCTCGTGCGCAGATGACTCAAGTGAGACTCGAGTTTTTAGATGACCAGAACCGTTTCATCACGAGAAACGTCAAGGGCCCAGTCAGGGAGGGTGATATCCTTATCTTGCTCGAATATGAGAGGGAAGCCAGAAGGTTGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S28e

Protein Analysis

53

Amino Acids

6.18

Weight (kDa)

9.93

Isoelectric Point (pI)

32.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S28e PF01200 1 - 51 3.3e-24 Ribosomal protein S28e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 24
AclWI GGATC 1 cut(s) 22
AfiI CCNNNNNNNGG 1 cut(s) 100
Alw26I GTCTC 1 cut(s) 34
AlwI GGATC 1 cut(s) 22
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 1 cut(s) 91
ApaI GGGCCC 1 cut(s) 95
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 2 cut(s) 25, 156
AspS9I GGNCC 2 cut(s) 91, 92
AsuHPI GGTGA 1 cut(s) 119
AvaI CYCGRG 1 cut(s) 44
BaeGI GKGCMC 1 cut(s) 95
BanII GRGCYC 1 cut(s) 95
BauI CACGAG 2 cut(s) 18, 76
BcoDI GTCTC 1 cut(s) 34
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 2 cut(s) 91, 92
BmiI GGNNCC 1 cut(s) 93
BmrI ACTGGG 1 cut(s) 89
BmuI ACTGGG 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 18
Bsc4I CCNNNNNNNGG 1 cut(s) 100
Bse1I ACTGG 2 cut(s) 16, 95
BseLI CCNNNNNNNGG 1 cut(s) 100
BseNI ACTGG 2 cut(s) 16, 95
BseSI GKGCMC 1 cut(s) 95
BshFI GGCC 1 cut(s) 93
BsiHKCI CYCGRG 1 cut(s) 44
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 1 cut(s) 100
BsmAI GTCTC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 1 cut(s) 93
BsoBI CYCGRG 1 cut(s) 44
Bsp120I GGGCCC 1 cut(s) 91
Bsp1286I GDGCHC 1 cut(s) 95
Bsp143I GATC 1 cut(s) 14
BspANI GGCC 1 cut(s) 93
BspLI GGNNCC 1 cut(s) 93
BspPI GGATC 1 cut(s) 22
BsrI ACTGG 2 cut(s) 16, 95
BssMI GATC 1 cut(s) 14
BssSI CACGAG 2 cut(s) 18, 76
Bst2BI CACGAG 2 cut(s) 18, 76
Bst4CI ACNGT 1 cut(s) 68
BstHHI GCGC 2 cut(s) 25, 156
BstKTI GATC 1 cut(s) 17
BstMAI GTCTC 1 cut(s) 34
BstMBI GATC 1 cut(s) 14
BstSLI GKGCMC 1 cut(s) 95
BstX2I RGATCY 1 cut(s) 14
BstYI RGATCY 1 cut(s) 14
BsuRI GGCC 1 cut(s) 93
CfoI GCGC 2 cut(s) 25, 156
Cfr13I GGNCC 2 cut(s) 91, 92
CviJI RGCY 2 cut(s) 93, 143
CviKI_1 RGCY 2 cut(s) 93, 143
DpnI GATC 1 cut(s) 16
DpnII GATC 1 cut(s) 14
Eco24I GRGCYC 1 cut(s) 95
Eco32I GATATC 1 cut(s) 112
Eco88I CYCGRG 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 91
EcoRV GATATC 1 cut(s) 112
EcoT38I GRGCYC 1 cut(s) 95
FaiI YATR 1 cut(s) 132
FaqI GGGAC 1 cut(s) 18
FriOI GRGCYC 1 cut(s) 95
FspI TGCGCA 1 cut(s) 24
GlaI GCGC 2 cut(s) 24, 155
HaeIII GGCC 1 cut(s) 93
HhaI GCGC 2 cut(s) 25, 156
Hin6I GCGC 2 cut(s) 23, 154
HinP1I GCGC 2 cut(s) 23, 154
HinfI GANTC 2 cut(s) 31, 42
HphI GGTGA 1 cut(s) 119
Hpy188III TCNNGA 1 cut(s) 76
HpyAV CCTTC 1 cut(s) 141
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4IV ACGT 1 cut(s) 84
HpySE526I ACGT 1 cut(s) 84
HspAI GCGC 2 cut(s) 23, 154
Kzo9I GATC 1 cut(s) 14
LpnPI CCDG 3 cut(s) 74, 85, 108
MaeII ACGT 1 cut(s) 84
MalI GATC 1 cut(s) 16
MboI GATC 1 cut(s) 14
MflI RGATCY 1 cut(s) 14
MhlI GDGCHC 1 cut(s) 95
MlyI GAGTC 2 cut(s) 25, 36
MnlI CCTC 2 cut(s) 97, 129
MroXI GAANNNNTTC 1 cut(s) 68
NdeII GATC 1 cut(s) 14
NlaIV GGNNCC 1 cut(s) 93
NsbI TGCGCA 1 cut(s) 24
PaeR7I CTCGAG 1 cut(s) 44
PdmI GAANNNNTTC 1 cut(s) 68
PleI GAGTC 2 cut(s) 25, 36
PpsI GAGTC 2 cut(s) 25, 36
PspN4I GGNNCC 1 cut(s) 93
PspOMI GGGCCC 1 cut(s) 91
PspPI GGNCC 2 cut(s) 91, 92
PspXI VCTCGAGB 1 cut(s) 44
PsuI RGATCY 1 cut(s) 14
Sau3AI GATC 1 cut(s) 14
Sau96I GGNCC 2 cut(s) 91, 92
SchI GAGTC 2 cut(s) 25, 36
SduI GDGCHC 1 cut(s) 95
SetI ASST 2 cut(s) 87, 152
Sfr274I CTCGAG 1 cut(s) 44
SlaI CTCGAG 1 cut(s) 44
SmlI CTYRAG 2 cut(s) 33, 44
SmoI CTYRAG 2 cut(s) 33, 44
TaaI ACNGT 1 cut(s) 68
TaiI ACGT 1 cut(s) 87
TaqI TCGA 2 cut(s) 45, 126
TspDTI ATGAA 1 cut(s) 61
XhoI CTCGAG 1 cut(s) 44
XmnI GAANNNNTTC 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.