Rorug06G0240900

40S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001317
Physical Location & Seq
Forward (+)
38438082 .. 38438923
842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0240900.1

Sequence Viewer

Length: 762 bp
ATGGCTCTGCGGTCCCTCAACCCTAGCCGCCTAGGCTTTGGGGATCTATGTCGCTCTAAGGTCGCAGCCGTGGGTTTTCCGGTTGCACTCCAACGTCGATGTTTGTCGACGAACCTATTGCTTTATGTCGGAGTTTTGTTCTTAGCAAGCCTACTTGTGGGGGACTCTCTCTGGTTCATCCACACCAGAACTGGTGAACCCCAGATCGATAATCAAGGTGAAAGATCTCAAATTACAAGGATCCAGAAGTCTTCACAGGAGATGGTAGAGACGGTGGTCATTCCACCGCGAGGCAACAACTGGGTCGACATTGAGGGCTCATCCTCGACACTGTCGCCGAAGCTGGTGTGCACCTTCAAGAACTATTGGGCGCCGTTTAAGAGGTGGCAATCGGGATGGGTGCCGTGGACAGCTAGGGCGACGCTATCACCTATTGGGACTATAGATGAATACGGCGATGCGCGTATCAATGGGTCACACTTGAAACAAATTTGGACACTAGTGCGTCCGTTTGCAGTTGTCACCGAGCTTATGACAGTATCAATGGCGGTGGTGCAATTGAATGTTGCCGGTCCGGCCTCCGGGTTCAAAGTGTGGGCATCTCCTTTCGGGGTGTCCATAGCAAAATGGAGTCTATGGCCCATTTCAGGCACCGACGATTTGCAGATATTAGCCAAGATGGTGTCTGCTGAAGAGCTCCTTAGTGTAATGTACTCAGGTAACCCTCCTAGCCAACGTTTGAAGGGCAAGGAAAAAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S28e

Protein Analysis

253

Amino Acids

27.95

Weight (kDa)

9.6

Isoelectric Point (pI)

47.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 370, 400, 650
AccI GTMKAC 2 cut(s) 107, 306
AccII CGCG 2 cut(s) 289, 463
AciI CCGC 4 cut(s) 10, 28, 287, 548
AclI AACGTT 1 cut(s) 736
AclWI GGATC 3 cut(s) 51, 235, 248
AcsI RAATTY 1 cut(s) 489
AcuI CTGAAG 1 cut(s) 711
AcyI GRCGYC 1 cut(s) 371
AfaI GTAC 1 cut(s) 713
AfiI CCNNNNNNNGG 4 cut(s) 157, 290, 581, 647
AgsI TTSAA 5 cut(s) 358, 484, 562, 589, 742
AhlI ACTAGT 1 cut(s) 499
AluBI AGCT 4 cut(s) 343, 413, 529, 697
AluI AGCT 4 cut(s) 343, 413, 529, 697
Alw21I GWGCWC 2 cut(s) 353, 699
Alw26I GTCTC 1 cut(s) 263
Alw44I GTGCAC 1 cut(s) 349
AlwI GGATC 3 cut(s) 51, 235, 248
AoxI GGCC 2 cut(s) 576, 638
ApaLI GTGCAC 1 cut(s) 349
ApeKI GCWGC 1 cut(s) 65
ApoI RAATTY 1 cut(s) 489
AspA2I CCTAGG 1 cut(s) 31
AspLEI GCGC 2 cut(s) 373, 463
AspS9I GGNCC 3 cut(s) 12, 572, 639
AsuC2I CCSGG 1 cut(s) 583
AsuHPI GGTGA 4 cut(s) 206, 230, 420, 514
AvaII GGWCC 2 cut(s) 12, 572
AvrII CCTAGG 1 cut(s) 31
BaeGI GKGCMC 1 cut(s) 353
BamHI GGATCC 1 cut(s) 240
BanI GGYRCC 3 cut(s) 370, 400, 650
BanII GRGCYC 2 cut(s) 320, 699
BbsI GAAGAC 1 cut(s) 243
Bbv12I GWGCWC 2 cut(s) 353, 699
BbvI GCAGC 1 cut(s) 77
BccI CCATC 3 cut(s) 256, 390, 673
BceAI ACGGC 4 cut(s) 53, 358, 388, 469
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BcnI CCSGG 1 cut(s) 583
BcoDI GTCTC 1 cut(s) 263
BcuI ACTAGT 1 cut(s) 499
BfaI CTAG 5 cut(s) 24, 32, 414, 500, 729
BfmI CTRYAG 1 cut(s) 441
BfoI RGCGCY 1 cut(s) 374
BglI GCCNNNNNGGC 2 cut(s) 33, 575
BglII AGATCT 1 cut(s) 224
BisI GCNGC 2 cut(s) 28, 66
BlnI CCTAGG 1 cut(s) 31
BlsI GCNGC 2 cut(s) 29, 67
Bme1390I CCNGG 1 cut(s) 583
Bme18I GGWCC 2 cut(s) 12, 572
BmgT120I GGNCC 3 cut(s) 12, 572, 639
BmiI GGNNCC 5 cut(s) 14, 242, 372, 402, 652
BmrFI CCNGG 1 cut(s) 583
BmrI ACTGGG 1 cut(s) 310
BmsI GCATC 2 cut(s) 448, 608
BmuI ACTGGG 1 cut(s) 310
BoxI GACNNNNGTC 1 cut(s) 275
BpiI GAAGAC 1 cut(s) 243
BpuMI CCSGG 1 cut(s) 583
Bsa29I ATCGAT 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 371
BsaJI CCNNGG 3 cut(s) 31, 69, 404
BsaWI WCCGGW 1 cut(s) 79
Bsc4I CCNNNNNNNGG 4 cut(s) 157, 290, 581, 647
Bse118I RCCGGY 1 cut(s) 569
Bse1I ACTGG 2 cut(s) 196, 305
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 3 cut(s) 31, 69, 404
BseGI GGATG 3 cut(s) 177, 320, 401
BseLI CCNNNNNNNGG 4 cut(s) 157, 290, 581, 647
BseMII CTCAG 1 cut(s) 729
BseNI ACTGG 2 cut(s) 196, 305
BseSI GKGCMC 1 cut(s) 353
BseXI GCAGC 1 cut(s) 77
Bsh1236I CGCG 2 cut(s) 289, 463
BshFI GGCC 2 cut(s) 578, 640
BshNI GGYRCC 3 cut(s) 370, 400, 650
BshVI ATCGAT 1 cut(s) 207
BsiHKAI GWGCWC 2 cut(s) 353, 699
BsiSI CCGG 4 cut(s) 80, 570, 575, 582
BslFI GGGAC 2 cut(s) 176, 451
BslI CCNNNNNNNGG 4 cut(s) 157, 290, 581, 647
BsmAI GTCTC 1 cut(s) 263
BsmBI CGTCTC 1 cut(s) 263
BsmFI GGGAC 2 cut(s) 176, 451
BsnI GGCC 2 cut(s) 578, 640
Bsp1286I GDGCHC 3 cut(s) 320, 353, 699
Bsp143I GATC 4 cut(s) 43, 204, 224, 240
BspACI CCGC 4 cut(s) 10, 28, 287, 548
BspANI GGCC 2 cut(s) 578, 640
BspCNI CTCAG 1 cut(s) 728
BspDI ATCGAT 1 cut(s) 207
BspFNI CGCG 2 cut(s) 289, 463
BspLI GGNNCC 5 cut(s) 14, 242, 372, 402, 652
BspPI GGATC 3 cut(s) 51, 235, 248
BspQI GCTCTTC 1 cut(s) 687
BspT107I GGYRCC 3 cut(s) 370, 400, 650
BsrFI RCCGGY 1 cut(s) 569
BsrI ACTGG 2 cut(s) 196, 305
BssAI RCCGGY 1 cut(s) 569
BssECI CCNNGG 3 cut(s) 31, 69, 404
BssMI GATC 4 cut(s) 43, 204, 224, 240
BssNI GRCGYC 1 cut(s) 371
BssT1I CCWWGG 1 cut(s) 31
Bst4CI ACNGT 3 cut(s) 274, 333, 538
Bst6I CTCTTC 1 cut(s) 687
BstACI GRCGYC 1 cut(s) 371
BstC8I GCNNGC 1 cut(s) 148
BstDEI CTNAG 4 cut(s) 57, 142, 701, 715
BstDSI CCRYGG 2 cut(s) 69, 404
BstEII GGTNACC 1 cut(s) 719
BstF5I GGATG 3 cut(s) 177, 320, 401
BstFNI CGCG 2 cut(s) 289, 463
BstH2I RGCGCY 1 cut(s) 374
BstHHI GCGC 2 cut(s) 373, 463
BstKTI GATC 4 cut(s) 46, 207, 227, 243
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 4 cut(s) 43, 204, 224, 240
BstMWI GCNNNNNNNGC 2 cut(s) 33, 575
BstPAI GACNNNNGTC 1 cut(s) 275
BstPI GGTNACC 1 cut(s) 719
BstSCI CCNGG 1 cut(s) 581
BstSFI CTRYAG 1 cut(s) 441
BstSLI GKGCMC 1 cut(s) 353
BstUI CGCG 2 cut(s) 289, 463
BstV1I GCAGC 1 cut(s) 77
BstV2I GAAGAC 1 cut(s) 243
BstX2I RGATCY 3 cut(s) 43, 224, 240
BstYI RGATCY 3 cut(s) 43, 224, 240
Bsu15I ATCGAT 1 cut(s) 207
BsuRI GGCC 2 cut(s) 578, 640
BsuTUI ATCGAT 1 cut(s) 207
BtgI CCRYGG 2 cut(s) 69, 404
BtgZI GCGATG 1 cut(s) 471
BtsCI GGATG 3 cut(s) 177, 320, 401
BtsIMutI CAGTG 1 cut(s) 329
Cac8I GCNNGC 1 cut(s) 148
CfoI GCGC 2 cut(s) 373, 463
Cfr10I RCCGGY 1 cut(s) 569
Cfr13I GGNCC 3 cut(s) 12, 572, 639
ClaI ATCGAT 1 cut(s) 207
CpoI CGGWCCG 1 cut(s) 572
CseI GACGC 2 cut(s) 430, 494
Csp6I GTAC 1 cut(s) 712
CspCI CAANNNNNGTGG 2 cut(s) 531, 566
CspI CGGWCCG 1 cut(s) 572
CviQI GTAC 1 cut(s) 712
DdeI CTNAG 4 cut(s) 57, 142, 701, 715
DinI GGCGCC 1 cut(s) 372
DpnI GATC 4 cut(s) 45, 206, 226, 242
DpnII GATC 4 cut(s) 43, 204, 224, 240
Eam1104I CTCTTC 1 cut(s) 687
EarI CTCTTC 1 cut(s) 687
Ecl136II GAGCTC 1 cut(s) 697
Eco130I CCWWGG 1 cut(s) 31
Eco24I GRGCYC 2 cut(s) 320, 699
Eco47I GGWCC 2 cut(s) 12, 572
Eco53kI GAGCTC 1 cut(s) 697
Eco57I CTGAAG 1 cut(s) 711
Eco91I GGTNACC 1 cut(s) 719
EcoICRI GAGCTC 1 cut(s) 697
EcoO65I GGTNACC 1 cut(s) 719
EcoT14I CCWWGG 1 cut(s) 31
EcoT38I GRGCYC 2 cut(s) 320, 699
EgeI GGCGCC 1 cut(s) 372
EheI GGCGCC 1 cut(s) 372
ErhI CCWWGG 1 cut(s) 31
Esp3I CGTCTC 1 cut(s) 263
FaiI YATR 7 cut(s) 49, 126, 443, 533, 620, 637, 760
FalI AAGNNNNNCTT 2 cut(s) 684, 716
FaqI GGGAC 2 cut(s) 176, 451
FblI GTMKAC 2 cut(s) 107, 306
Fnu4HI GCNGC 2 cut(s) 28, 66
FokI GGATG 3 cut(s) 164, 307, 408
FriOI GRGCYC 2 cut(s) 320, 699
Fsp4HI GCNGC 2 cut(s) 28, 66
FspBI CTAG 5 cut(s) 24, 32, 414, 500, 729
GlaI GCGC 2 cut(s) 372, 462
GluI GCNGC 2 cut(s) 28, 66
HaeII RGCGCY 1 cut(s) 374
HaeIII GGCC 2 cut(s) 578, 640
HapII CCGG 4 cut(s) 80, 570, 575, 582
HgaI GACGC 2 cut(s) 430, 494
HhaI GCGC 2 cut(s) 373, 463
Hin1I GRCGYC 1 cut(s) 371
Hin6I GCGC 2 cut(s) 371, 461
HinP1I GCGC 2 cut(s) 371, 461
HincII GTYRAC 2 cut(s) 108, 307
HindII GTYRAC 2 cut(s) 108, 307
HinfI GANTC 2 cut(s) 164, 631
HpaII CCGG 4 cut(s) 80, 570, 575, 582
HphI GGTGA 4 cut(s) 206, 230, 420, 514
Hpy166II GTNNAC 5 cut(s) 108, 197, 307, 351, 408
Hpy188I TCNGA 1 cut(s) 131
Hpy188III TCNNGA 3 cut(s) 244, 358, 393
Hpy8I GTNNAC 5 cut(s) 108, 197, 307, 351, 408
Hpy99I CGWCG 4 cut(s) 99, 112, 424, 659
HpyAV CCTTC 2 cut(s) 364, 736
HpyCH4III ACNGT 3 cut(s) 274, 333, 538
HpyCH4IV ACGT 2 cut(s) 94, 736
HpyCH4V TGCA 5 cut(s) 86, 351, 515, 556, 664
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 575
HpyF3I CTNAG 4 cut(s) 57, 142, 701, 715
HpySE526I ACGT 2 cut(s) 94, 736
Hsp92I GRCGYC 1 cut(s) 371
HspAI GCGC 2 cut(s) 371, 461
KasI GGCGCC 1 cut(s) 370
Kzo9I GATC 4 cut(s) 43, 204, 224, 240
LguI GCTCTTC 1 cut(s) 687
LmnI GCTCC 1 cut(s) 702
Lsp1109I GCAGC 1 cut(s) 77
LweI GCATC 2 cut(s) 448, 608
MaeI CTAG 5 cut(s) 24, 32, 414, 500, 729
MaeII ACGT 2 cut(s) 94, 736
MaeIII GTNAC 3 cut(s) 474, 520, 719
MalI GATC 4 cut(s) 45, 206, 226, 242
MboI GATC 4 cut(s) 43, 204, 224, 240
MboII GAAGA 2 cut(s) 243, 704
MfeI CAATTG 1 cut(s) 557
MflI RGATCY 3 cut(s) 43, 224, 240
MhlI GDGCHC 3 cut(s) 320, 353, 699
MluCI AATT 3 cut(s) 231, 489, 557
Mly113I GGCGCC 1 cut(s) 371
MlyI GAGTC 2 cut(s) 158, 640
MmeI TCCRAC 2 cut(s) 109, 115
MnlI CCTC 7 cut(s) 26, 284, 307, 334, 375, 589, 735
MseI TTAA 1 cut(s) 378
MspI CCGG 4 cut(s) 80, 570, 575, 582
MspR9I CCNGG 1 cut(s) 583
MunI CAATTG 1 cut(s) 557
MvnI CGCG 2 cut(s) 289, 463
MwoI GCNNNNNNNGC 2 cut(s) 33, 575
NarI GGCGCC 1 cut(s) 371
NciI CCSGG 1 cut(s) 583
NdeII GATC 4 cut(s) 43, 204, 224, 240
NlaIV GGNNCC 5 cut(s) 14, 242, 372, 402, 652
NmuCI GTSAC 2 cut(s) 474, 520
PciSI GCTCTTC 1 cut(s) 687
PflFI GACNNNGTC 1 cut(s) 331
PkrI GCNGC 2 cut(s) 29, 67
PleI GAGTC 2 cut(s) 158, 639
PluTI GGCGCC 1 cut(s) 374
PpsI GAGTC 2 cut(s) 158, 639
PshAI GACNNNNGTC 1 cut(s) 275
Psp124BI GAGCTC 1 cut(s) 699
Psp1406I AACGTT 1 cut(s) 736
PspEI GGTNACC 1 cut(s) 719
PspN4I GGNNCC 5 cut(s) 14, 242, 372, 402, 652
PspPI GGNCC 3 cut(s) 12, 572, 639
PsuI RGATCY 3 cut(s) 43, 224, 240
PsyI GACNNNGTC 1 cut(s) 331
RsaI GTAC 1 cut(s) 713
RsaNI GTAC 1 cut(s) 712
Rsr2I CGGWCCG 1 cut(s) 572
RsrII CGGWCCG 1 cut(s) 572
SacI GAGCTC 1 cut(s) 699
SalI GTCGAC 2 cut(s) 106, 305
SapI GCTCTTC 1 cut(s) 687
SaqAI TTAA 1 cut(s) 378
SatI GCNGC 2 cut(s) 28, 66
Sau3AI GATC 4 cut(s) 43, 204, 224, 240
Sau96I GGNCC 3 cut(s) 12, 572, 639
SchI GAGTC 2 cut(s) 158, 640
ScrFI CCNGG 1 cut(s) 583
SduI GDGCHC 3 cut(s) 320, 353, 699
SfaNI GCATC 2 cut(s) 448, 608
SfcI CTRYAG 1 cut(s) 441
SfoI GGCGCC 1 cut(s) 372
SinI GGWCC 2 cut(s) 12, 572
SpeI ACTAGT 1 cut(s) 499
Sse9I AATT 3 cut(s) 231, 489, 557
SsiI CCGC 4 cut(s) 10, 28, 287, 548
SspDI GGCGCC 1 cut(s) 370
SspMI CTAG 5 cut(s) 24, 32, 414, 500, 729
SstI GAGCTC 1 cut(s) 699
StyD4I CCNGG 1 cut(s) 581
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 3 cut(s) 274, 333, 538
TaiI ACGT 2 cut(s) 97, 739
TaqI TCGA 5 cut(s) 97, 107, 207, 306, 326
TasI AATT 3 cut(s) 231, 489, 557
TatI WGTACW 1 cut(s) 711
TauI GCSGC 1 cut(s) 30
Tru1I TTAA 1 cut(s) 378
Tru9I TTAA 1 cut(s) 378
TscAI CASTG 1 cut(s) 336
TseFI GTSAC 2 cut(s) 474, 520
TseI GCWGC 1 cut(s) 65
Tsp45I GTSAC 2 cut(s) 474, 520
TspDTI ATGAA 2 cut(s) 166, 462
TspGWI ACGGA 1 cut(s) 498
TspRI CASTG 1 cut(s) 336
Tth111I GACNNNGTC 1 cut(s) 331
VneI GTGCAC 1 cut(s) 349
VpaK11BI GGWCC 2 cut(s) 12, 572
XapI RAATTY 1 cut(s) 489
XcmI CCANNNNNNNNNTGG 1 cut(s) 188
XmaJI CCTAGG 1 cut(s) 31
XmiI GTMKAC 2 cut(s) 107, 306
XspI CTAG 5 cut(s) 24, 32, 414, 500, 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.