MD14G1048300.v1.1

40S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001317
Physical Location & Seq
Forward (+)
4614810 .. 4616552
1743 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1048300.v1.1.491

Sequence Viewer

Length: 159 bp
ATGGGACGGACTGGATCTCGTGGGCAGGTGACTCAAGTGAGAGTCAAGTTTCTGGATGACCAGAACCGTTTCATCATGAGGAATGTCAAGGGACCGGTCAGGGAGGGGGATATCCTTACCTTGCTCGAGTCCGAGCGGGAGGCAAGAAGGTTGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S28e

Protein Analysis

53

Amino Acids

6.09

Weight (kDa)

11.42

Isoelectric Point (pI)

31.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S28e PF01200 1 - 51 5.2e-28 Ribosomal protein S28e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 16
Acc36I ACCTGC 1 cut(s) 16
AccBSI CCGCTC 1 cut(s) 136
AciI CCGC 2 cut(s) 136, 154
AclWI GGATC 1 cut(s) 22
AgeI ACCGGT 1 cut(s) 94
AlwI GGATC 1 cut(s) 22
Ama87I CYCGRG 1 cut(s) 125
AsiGI ACCGGT 1 cut(s) 94
Asp700I GAANNNNTTC 1 cut(s) 68
AspS9I GGNCC 1 cut(s) 92
AsuHPI GGTGA 1 cut(s) 40
AvaI CYCGRG 1 cut(s) 125
AvaII GGWCC 1 cut(s) 92
BauI CACGAG 1 cut(s) 18
BfuAI ACCTGC 1 cut(s) 16
Bme18I GGWCC 1 cut(s) 92
BmeT110I CYCGRG 1 cut(s) 125
BmgT120I GGNCC 1 cut(s) 92
BmiI GGNNCC 1 cut(s) 93
BpuEI CTTGAG 1 cut(s) 18
BsaWI WCCGGW 1 cut(s) 94
Bse118I RCCGGY 1 cut(s) 94
Bse1I ACTGG 1 cut(s) 16
BseGI GGATG 1 cut(s) 61
BseNI ACTGG 1 cut(s) 16
BshTI ACCGGT 1 cut(s) 94
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 1 cut(s) 95
BslFI GGGAC 2 cut(s) 18, 105
BsmFI GGGAC 2 cut(s) 18, 105
BsoBI CYCGRG 1 cut(s) 125
Bsp143I GATC 1 cut(s) 14
BspACI CCGC 2 cut(s) 136, 154
BspHI TCATGA 1 cut(s) 75
BspLI GGNNCC 1 cut(s) 93
BspMI ACCTGC 1 cut(s) 16
BspPI GGATC 1 cut(s) 22
BsrBI CCGCTC 1 cut(s) 136
BsrFI RCCGGY 1 cut(s) 94
BsrI ACTGG 1 cut(s) 16
BssAI RCCGGY 1 cut(s) 94
BssMI GATC 1 cut(s) 14
BssSI CACGAG 1 cut(s) 18
Bst2BI CACGAG 1 cut(s) 18
Bst4CI ACNGT 1 cut(s) 68
BstF5I GGATG 1 cut(s) 61
BstKTI GATC 1 cut(s) 17
BstMBI GATC 1 cut(s) 14
BstX2I RGATCY 1 cut(s) 14
BstYI RGATCY 1 cut(s) 14
BtsCI GGATG 1 cut(s) 61
BveI ACCTGC 1 cut(s) 16
CciI TCATGA 1 cut(s) 75
Cfr10I RCCGGY 1 cut(s) 94
Cfr13I GGNCC 1 cut(s) 92
CspAI ACCGGT 1 cut(s) 94
CviAII CATG 1 cut(s) 76
DpnI GATC 1 cut(s) 16
DpnII GATC 1 cut(s) 14
Eco32I GATATC 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 92
Eco88I CYCGRG 1 cut(s) 125
EcoRV GATATC 1 cut(s) 112
FaeI CATG 1 cut(s) 79
FaiI YATR 1 cut(s) 77
FaqI GGGAC 2 cut(s) 18, 105
FatI CATG 1 cut(s) 75
FauI CCCGC 1 cut(s) 129
FokI GGATG 1 cut(s) 68
HapII CCGG 1 cut(s) 95
Hin1II CATG 1 cut(s) 79
HinfI GANTC 3 cut(s) 31, 42, 128
HpaII CCGG 1 cut(s) 95
HphI GGTGA 1 cut(s) 40
Hpy188I TCNGA 1 cut(s) 133
Hpy188III TCNNGA 2 cut(s) 53, 76
HpyAV CCTTC 1 cut(s) 141
HpyCH4III ACNGT 1 cut(s) 68
Hsp92II CATG 1 cut(s) 79
Kzo9I GATC 1 cut(s) 14
LpnPI CCDG 5 cut(s) 11, 38, 74, 85, 108
MaeIII GTNAC 1 cut(s) 28
MalI GATC 1 cut(s) 16
MbiI CCGCTC 1 cut(s) 136
MboI GATC 1 cut(s) 14
MflI RGATCY 1 cut(s) 14
MlyI GAGTC 3 cut(s) 25, 51, 137
MnlI CCTC 3 cut(s) 72, 97, 133
MroXI GAANNNNTTC 1 cut(s) 68
MspI CCGG 1 cut(s) 95
NdeII GATC 1 cut(s) 14
NlaIII CATG 1 cut(s) 79
NlaIV GGNNCC 1 cut(s) 93
NmuCI GTSAC 1 cut(s) 28
PaeR7I CTCGAG 1 cut(s) 125
PagI TCATGA 1 cut(s) 75
PaqCI CACCTGC 1 cut(s) 16
PdmI GAANNNNTTC 1 cut(s) 68
PinAI ACCGGT 1 cut(s) 94
PleI GAGTC 3 cut(s) 25, 50, 136
PpsI GAGTC 3 cut(s) 25, 50, 136
PspN4I GGNNCC 1 cut(s) 93
PspPI GGNCC 1 cut(s) 92
PspXI VCTCGAGB 1 cut(s) 125
PsuI RGATCY 1 cut(s) 14
Sau3AI GATC 1 cut(s) 14
Sau96I GGNCC 1 cut(s) 92
SchI GAGTC 3 cut(s) 25, 51, 137
SetI ASST 3 cut(s) 30, 122, 152
Sfr274I CTCGAG 1 cut(s) 125
SinI GGWCC 1 cut(s) 92
SlaI CTCGAG 1 cut(s) 125
SmlI CTYRAG 2 cut(s) 33, 125
SmoI CTYRAG 2 cut(s) 33, 125
SsiI CCGC 2 cut(s) 136, 154
TaaI ACNGT 1 cut(s) 68
TaqI TCGA 1 cut(s) 126
TseFI GTSAC 1 cut(s) 28
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 1 cut(s) 61
TspGWI ACGGA 1 cut(s) 22
VpaK11BI GGWCC 1 cut(s) 92
XhoI CTCGAG 1 cut(s) 125
XmnI GAANNNNTTC 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.