MD12G1079700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001257
Physical Location & Seq
Forward (+)
9726494 .. 9728552
2059 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1079700.v1.1.491

Sequence Viewer

Length: 711 bp
ATGGCATCAACACTCGGTGCTTTGTTTCAAGATCAGAATCTGAGTGTTCACTCTAAGGGAGCTTCTCTTGTGGGGAAGGGTGATGCCTTGAAAACAAAAAAGAAAGGCGGGCTTGGTGGAAGGAAACCGCTTGGTGATTTATCGAATTCAGGGAAGCCTGCTGCTCTGACTCAGGCATCAAAAAAGCAGTCTTCCAAGGTAATCATTTGTGGTGATGCAAGCAATAACAAAGGCTTGTCTAAAGCCTCAGATAAAGTTCAGACCAGTACCAGGAACGCGTTACGTGATATTTCAAACACACGGGATGCACCGGTGAAGAACAACACAAAGCAGAGGGTTATGCCAAAGCAGCCTGTTTGTCCTGATAATATTGCAGAGGAAGGGTTTCTGCACAATCATCAAGAGTGCATGAAGGCACAGGACATGGATGAGATTCATCAGTTTCGTCAGTTTCTGATGGCACTTGGAGTACATAGTGATTCTTCCGAGAAGCTTACATCTTCATGTGCATCTTCACAGCCAAGTAAATCTGAGCCCCAGAGTCCCTCGAAGTATATGGGTTTGGAAGAGATACCTGAGGATCGAAACCCCTGGCAGTCTGACAATCTCGACTCTCCACCTCCTTGCAAGTCTCCAAAGTCACCATATGGCTATACTGATTCCCTTTTGATTTGGGAGGACTGTGACTTCAAGTTGACGAACACACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

237

Amino Acids

25.6

Weight (kDa)

8.65

Isoelectric Point (pI)

60.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 278
AciI CCGC 2 cut(s) 108, 128
AclWI GGATC 1 cut(s) 588
AcsI RAATTY 1 cut(s) 145
AdeI CACNNNGTG 1 cut(s) 17
AfaI GTAC 2 cut(s) 268, 471
AfiI CCNNNNNNNGG 1 cut(s) 270
AflIII ACRYGT 1 cut(s) 276
AgeI ACCGGT 1 cut(s) 310
AgsI TTSAA 4 cut(s) 29, 91, 294, 691
AjnI CCWGG 2 cut(s) 269, 590
AluBI AGCT 2 cut(s) 62, 493
AluI AGCT 2 cut(s) 62, 493
Alw26I GTCTC 1 cut(s) 636
AlwI GGATC 1 cut(s) 588
AlwNI CAGNNNCTG 2 cut(s) 40, 454
ApeKI GCWGC 2 cut(s) 161, 349
ApoI RAATTY 1 cut(s) 145
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
AsiGI ACCGGT 1 cut(s) 310
Asp700I GAANNNNTTC 1 cut(s) 384
AsuHPI GGTGA 5 cut(s) 92, 146, 224, 325, 633
AxyI CCTNAGG 1 cut(s) 576
BanII GRGCYC 1 cut(s) 537
BbsI GAAGAC 1 cut(s) 183
BbvI GCAGC 2 cut(s) 148, 361
BccI CCATC 1 cut(s) 451
BciT130I CCWGG 2 cut(s) 271, 592
BcoDI GTCTC 1 cut(s) 636
BisI GCNGC 2 cut(s) 162, 350
BlsI GCNGC 2 cut(s) 163, 351
Bme1390I CCNGG 2 cut(s) 271, 592
BmrFI CCNGG 2 cut(s) 271, 592
BmsI GCATC 6 cut(s) 14, 73, 185, 205, 295, 518
BpiI GAAGAC 1 cut(s) 183
BsaAI YACGTR 1 cut(s) 284
BsaBI GATNNNNATC 1 cut(s) 36
BsaJI CCNNGG 2 cut(s) 195, 590
BsaWI WCCGGW 1 cut(s) 310
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse118I RCCGGY 1 cut(s) 310
Bse1I ACTGG 1 cut(s) 264
Bse21I CCTNAGG 1 cut(s) 576
Bse8I GATNNNNATC 1 cut(s) 36
BseBI CCWGG 2 cut(s) 271, 592
BseDI CCNNGG 2 cut(s) 195, 590
BseGI GGATG 2 cut(s) 310, 433
BseJI GATNNNNATC 1 cut(s) 36
BseLI CCNNNNNNNGG 1 cut(s) 270
BseMII CTCAG 5 cut(s) 32, 185, 261, 522, 567
BseNI ACTGG 1 cut(s) 264
BseXI GCAGC 2 cut(s) 148, 361
BsgI GTGCAG 1 cut(s) 374
Bsh1236I CGCG 1 cut(s) 278
BshTI ACCGGT 1 cut(s) 310
BsiSI CCGG 1 cut(s) 311
BslFI GGGAC 1 cut(s) 528
BslI CCNNNNNNNGG 1 cut(s) 270
BsmAI GTCTC 1 cut(s) 636
BsmFI GGGAC 1 cut(s) 528
Bsp1286I GDGCHC 1 cut(s) 537
Bsp143I GATC 2 cut(s) 31, 580
BspACI CCGC 2 cut(s) 108, 128
BspCNI CTCAG 5 cut(s) 33, 184, 260, 523, 568
BspFNI CGCG 1 cut(s) 278
BspPI GGATC 1 cut(s) 588
BsrFI RCCGGY 1 cut(s) 310
BsrI ACTGG 1 cut(s) 264
BssAI RCCGGY 1 cut(s) 310
BssECI CCNNGG 2 cut(s) 195, 590
BssMI GATC 2 cut(s) 31, 580
BssT1I CCWWGG 1 cut(s) 195
Bst2UI CCWGG 2 cut(s) 271, 592
Bst4CI ACNGT 1 cut(s) 683
Bst6I CTCTTC 1 cut(s) 561
BstBAI YACGTR 1 cut(s) 284
BstC8I GCNNGC 3 cut(s) 110, 159, 220
BstDEI CTNAG 6 cut(s) 41, 54, 171, 247, 531, 576
BstF5I GGATG 2 cut(s) 310, 433
BstFNI CGCG 1 cut(s) 278
BstKTI GATC 2 cut(s) 34, 583
BstMAI GTCTC 1 cut(s) 636
BstMBI GATC 2 cut(s) 31, 580
BstMWI GCNNNNNNNGC 1 cut(s) 349
BstNI CCWGG 2 cut(s) 271, 592
BstSCI CCNGG 2 cut(s) 269, 590
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 2 cut(s) 148, 361
BstV2I GAAGAC 1 cut(s) 183
Bsu36I CCTNAGG 1 cut(s) 576
BtsCI GGATG 2 cut(s) 310, 433
Cac8I GCNNGC 3 cut(s) 110, 159, 220
CaiI CAGNNNCTG 2 cut(s) 40, 454
Cfr10I RCCGGY 1 cut(s) 310
Csp6I GTAC 2 cut(s) 267, 470
CspAI ACCGGT 1 cut(s) 310
CviAII CATG 3 cut(s) 409, 424, 504
CviQI GTAC 2 cut(s) 267, 470
DdeI CTNAG 6 cut(s) 41, 54, 171, 247, 531, 576
DpnI GATC 2 cut(s) 33, 582
DpnII GATC 2 cut(s) 31, 580
DraIII CACNNNGTG 1 cut(s) 17
Eam1104I CTCTTC 1 cut(s) 561
EarI CTCTTC 1 cut(s) 561
Eco130I CCWWGG 1 cut(s) 195
Eco24I GRGCYC 1 cut(s) 537
Eco81I CCTNAGG 1 cut(s) 576
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 2 cut(s) 269, 590
EcoT14I CCWWGG 1 cut(s) 195
EcoT38I GRGCYC 1 cut(s) 537
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 3 cut(s) 412, 427, 507
FalI AAGNNNNNCTT 2 cut(s) 96, 128
FaqI GGGAC 1 cut(s) 528
FatI CATG 3 cut(s) 408, 423, 503
FauI CCCGC 1 cut(s) 101
FauNDI CATATG 1 cut(s) 646
Fnu4HI GCNGC 2 cut(s) 162, 350
FokI GGATG 2 cut(s) 317, 440
FriOI GRGCYC 1 cut(s) 537
Fsp4HI GCNGC 2 cut(s) 162, 350
GluI GCNGC 2 cut(s) 162, 350
HapII CCGG 1 cut(s) 311
Hin1II CATG 3 cut(s) 412, 427, 507
HincII GTYRAC 1 cut(s) 696
HindII GTYRAC 1 cut(s) 696
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 7 cut(s) 37, 169, 433, 479, 541, 611, 659
HpaII CCGG 1 cut(s) 311
HphI GGTGA 5 cut(s) 92, 146, 224, 325, 633
Hpy166II GTNNAC 2 cut(s) 49, 696
Hpy188I TCNGA 9 cut(s) 36, 42, 168, 250, 261, 456, 487, 532, 601
Hpy188III TCNNGA 4 cut(s) 29, 362, 401, 608
Hpy8I GTNNAC 2 cut(s) 49, 696
HpyAV CCTTC 4 cut(s) 70, 114, 374, 406
HpyCH4III ACNGT 1 cut(s) 683
HpyCH4IV ACGT 1 cut(s) 283
HpyCH4V TGCA 7 cut(s) 218, 308, 374, 391, 408, 509, 627
HpyF10VI GCNNNNNNNGC 1 cut(s) 349
HpyF3I CTNAG 6 cut(s) 41, 54, 171, 247, 531, 576
HpySE526I ACGT 1 cut(s) 283
Hsp92II CATG 3 cut(s) 412, 427, 507
Kzo9I GATC 2 cut(s) 31, 580
LmnI GCTCC 1 cut(s) 59
Lsp1109I GCAGC 2 cut(s) 148, 361
LweI GCATC 6 cut(s) 14, 73, 185, 205, 295, 518
MaeII ACGT 1 cut(s) 283
MaeIII GTNAC 3 cut(s) 279, 639, 683
MalI GATC 2 cut(s) 33, 582
MboI GATC 2 cut(s) 31, 580
MboII GAAGA 6 cut(s) 183, 328, 474, 492, 504, 578
MhlI GDGCHC 1 cut(s) 537
MluCI AATT 1 cut(s) 145
MluI ACGCGT 1 cut(s) 276
MlyI GAGTC 3 cut(s) 163, 550, 605
MnlI CCTC 7 cut(s) 256, 327, 370, 556, 571, 630, 670
MroXI GAANNNNTTC 1 cut(s) 384
MslI CAYNNNNRTG 1 cut(s) 502
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 2 cut(s) 271, 592
MvaI CCWGG 2 cut(s) 271, 592
MvnI CGCG 1 cut(s) 278
MwoI GCNNNNNNNGC 1 cut(s) 349
NdeI CATATG 1 cut(s) 646
NdeII GATC 2 cut(s) 31, 580
NlaIII CATG 3 cut(s) 412, 427, 507
NmuCI GTSAC 2 cut(s) 639, 683
PdmI GAANNNNTTC 1 cut(s) 384
PfeI GAWTC 4 cut(s) 37, 433, 479, 659
PinAI ACCGGT 1 cut(s) 310
PkrI GCNGC 2 cut(s) 163, 351
PleI GAGTC 3 cut(s) 163, 549, 605
PpsI GAGTC 3 cut(s) 163, 549, 605
Ppu21I YACGTR 1 cut(s) 284
Psp6I CCWGG 2 cut(s) 269, 590
PspGI CCWGG 2 cut(s) 269, 590
PstNI CAGNNNCTG 2 cut(s) 40, 454
RsaI GTAC 2 cut(s) 268, 471
RsaNI GTAC 2 cut(s) 267, 470
RseI CAYNNNNRTG 1 cut(s) 502
SatI GCNGC 2 cut(s) 162, 350
Sau3AI GATC 2 cut(s) 31, 580
SchI GAGTC 3 cut(s) 163, 550, 605
ScrFI CCNGG 2 cut(s) 271, 592
SduI GDGCHC 1 cut(s) 537
SetI ASST 6 cut(s) 64, 201, 286, 495, 577, 622
SfaNI GCATC 6 cut(s) 14, 73, 185, 205, 295, 518
SgrAI CRCCGGYG 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 502
Sse9I AATT 1 cut(s) 145
SsiI CCGC 2 cut(s) 108, 128
SspI AATATT 1 cut(s) 370
StyD4I CCNGG 2 cut(s) 269, 590
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 1 cut(s) 683
TaiI ACGT 1 cut(s) 286
TaqI TCGA 4 cut(s) 143, 548, 583, 609
TasI AATT 1 cut(s) 145
TatI WGTACW 1 cut(s) 469
TfiI GAWTC 4 cut(s) 37, 433, 479, 659
TseFI GTSAC 2 cut(s) 639, 683
TseI GCWGC 2 cut(s) 161, 349
Tsp45I GTSAC 2 cut(s) 639, 683
TspDTI ATGAA 3 cut(s) 425, 425, 492
XapI RAATTY 1 cut(s) 145
XmnI GAANNNNTTC 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.