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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 92 |
| AgsI | TTSAA | 2 cut(s) | 68, 256 |
| AjnI | CCWGG | 1 cut(s) | 155 |
| AluBI | AGCT | 3 cut(s) | 58, 172, 259 |
| AluI | AGCT | 3 cut(s) | 58, 172, 259 |
| Alw21I | GWGCWC | 1 cut(s) | 76 |
| Alw26I | GTCTC | 1 cut(s) | 201 |
| Alw44I | GTGCAC | 1 cut(s) | 72 |
| AlwI | GGATC | 1 cut(s) | 92 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 19 |
| Ama87I | CYCGRG | 2 cut(s) | 101, 112 |
| ApaLI | GTGCAC | 1 cut(s) | 72 |
| AvaI | CYCGRG | 2 cut(s) | 101, 112 |
| BaeGI | GKGCMC | 1 cut(s) | 76 |
| Bbv12I | GWGCWC | 1 cut(s) | 76 |
| BccI | CCATC | 1 cut(s) | 256 |
| BciT130I | CCWGG | 1 cut(s) | 157 |
| BcoDI | GTCTC | 1 cut(s) | 201 |
| BfaI | CTAG | 1 cut(s) | 35 |
| Bme1390I | CCNGG | 1 cut(s) | 157 |
| BmeT110I | CYCGRG | 2 cut(s) | 101, 112 |
| BmrFI | CCNGG | 1 cut(s) | 157 |
| BmsI | GCATC | 1 cut(s) | 126 |
| BoxI | GACNNNNGTC | 1 cut(s) | 9 |
| Bpu10I | CCTNAGC | 1 cut(s) | 141 |
| BsaJI | CCNNGG | 3 cut(s) | 86, 113, 155 |
| Bse3DI | GCAATG | 1 cut(s) | 223 |
| BseBI | CCWGG | 1 cut(s) | 157 |
| BseDI | CCNNGG | 3 cut(s) | 86, 113, 155 |
| BseMI | GCAATG | 1 cut(s) | 223 |
| BseMII | CTCAG | 1 cut(s) | 132 |
| BseSI | GKGCMC | 1 cut(s) | 76 |
| BsiHKAI | GWGCWC | 1 cut(s) | 76 |
| BsiHKCI | CYCGRG | 2 cut(s) | 101, 112 |
| BslFI | GGGAC | 2 cut(s) | 93, 254 |
| BsmAI | GTCTC | 1 cut(s) | 201 |
| BsmFI | GGGAC | 2 cut(s) | 93, 254 |
| BsoBI | CYCGRG | 2 cut(s) | 101, 112 |
| Bsp1286I | GDGCHC | 1 cut(s) | 76 |
| Bsp143I | GATC | 1 cut(s) | 97 |
| BspCNI | CTCAG | 1 cut(s) | 133 |
| BspPI | GGATC | 1 cut(s) | 92 |
| BsrDI | GCAATG | 1 cut(s) | 223 |
| BssECI | CCNNGG | 3 cut(s) | 86, 113, 155 |
| BssMI | GATC | 1 cut(s) | 97 |
| BssT1I | CCWWGG | 1 cut(s) | 86 |
| Bst2UI | CCWGG | 1 cut(s) | 157 |
| Bst4CI | ACNGT | 1 cut(s) | 248 |
| Bst6I | CTCTTC | 1 cut(s) | 126 |
| BstDEI | CTNAG | 1 cut(s) | 141 |
| BstKTI | GATC | 1 cut(s) | 100 |
| BstMAI | GTCTC | 1 cut(s) | 201 |
| BstMBI | GATC | 1 cut(s) | 97 |
| BstNI | CCWGG | 1 cut(s) | 157 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 9 |
| BstSCI | CCNGG | 1 cut(s) | 155 |
| BstSLI | GKGCMC | 1 cut(s) | 76 |
| BtsI | GCAGTG | 1 cut(s) | 80 |
| BtsIMutI | CAGTG | 1 cut(s) | 80 |
| CaiI | CAGNNNCTG | 1 cut(s) | 19 |
| CviAII | CATG | 3 cut(s) | 7, 95, 146 |
| CviJI | RGCY | 4 cut(s) | 58, 160, 172, 259 |
| CviKI_1 | RGCY | 4 cut(s) | 58, 160, 172, 259 |
| DdeI | CTNAG | 1 cut(s) | 141 |
| DpnI | GATC | 1 cut(s) | 99 |
| DpnII | GATC | 1 cut(s) | 97 |
| Eam1104I | CTCTTC | 1 cut(s) | 126 |
| EarI | CTCTTC | 1 cut(s) | 126 |
| Eco130I | CCWWGG | 1 cut(s) | 86 |
| Eco88I | CYCGRG | 2 cut(s) | 101, 112 |
| EcoRII | CCWGG | 1 cut(s) | 155 |
| EcoT14I | CCWWGG | 1 cut(s) | 86 |
| ErhI | CCWWGG | 1 cut(s) | 86 |
| FaeI | CATG | 3 cut(s) | 10, 98, 149 |
| FaiI | YATR | 4 cut(s) | 8, 96, 147, 274 |
| FaqI | GGGAC | 2 cut(s) | 93, 254 |
| FatI | CATG | 3 cut(s) | 6, 94, 145 |
| FspBI | CTAG | 1 cut(s) | 35 |
| Hin1II | CATG | 3 cut(s) | 10, 98, 149 |
| HindIII | AAGCTT | 2 cut(s) | 56, 170 |
| HinfI | GANTC | 3 cut(s) | 106, 176, 224 |
| Hpy166II | GTNNAC | 1 cut(s) | 74 |
| Hpy188I | TCNGA | 3 cut(s) | 21, 32, 166 |
| Hpy188III | TCNNGA | 1 cut(s) | 101 |
| Hpy8I | GTNNAC | 1 cut(s) | 74 |
| HpyCH4III | ACNGT | 1 cut(s) | 248 |
| HpyCH4V | TGCA | 3 cut(s) | 74, 192, 216 |
| HpyF3I | CTNAG | 1 cut(s) | 141 |
| Hsp92II | CATG | 3 cut(s) | 10, 98, 149 |
| Kzo9I | GATC | 1 cut(s) | 97 |
| LpnPI | CCDG | 3 cut(s) | 142, 153, 169 |
| LweI | GCATC | 1 cut(s) | 126 |
| MaeI | CTAG | 1 cut(s) | 35 |
| MaeIII | GTNAC | 2 cut(s) | 204, 248 |
| MalI | GATC | 1 cut(s) | 99 |
| MboI | GATC | 1 cut(s) | 97 |
| MboII | GAAGA | 2 cut(s) | 39, 143 |
| MhlI | GDGCHC | 1 cut(s) | 76 |
| MluCI | AATT | 1 cut(s) | 211 |
| MlyI | GAGTC | 2 cut(s) | 115, 170 |
| MnlI | CCTC | 3 cut(s) | 87, 108, 195 |
| MspR9I | CCNGG | 1 cut(s) | 157 |
| MvaI | CCWGG | 1 cut(s) | 157 |
| NdeII | GATC | 1 cut(s) | 97 |
| NlaIII | CATG | 3 cut(s) | 10, 98, 149 |
| NmuCI | GTSAC | 2 cut(s) | 204, 248 |
| PfeI | GAWTC | 1 cut(s) | 224 |
| PleI | GAGTC | 2 cut(s) | 114, 170 |
| PpsI | GAGTC | 2 cut(s) | 114, 170 |
| PshAI | GACNNNNGTC | 1 cut(s) | 9 |
| Psp6I | CCWGG | 1 cut(s) | 155 |
| PspGI | CCWGG | 1 cut(s) | 155 |
| PstNI | CAGNNNCTG | 1 cut(s) | 19 |
| Sau3AI | GATC | 1 cut(s) | 97 |
| SchI | GAGTC | 2 cut(s) | 115, 170 |
| ScrFI | CCNGG | 1 cut(s) | 157 |
| SduI | GDGCHC | 1 cut(s) | 76 |
| SetI | ASST | 5 cut(s) | 60, 79, 174, 187, 261 |
| SfaNI | GCATC | 1 cut(s) | 126 |
| Sse9I | AATT | 1 cut(s) | 211 |
| SspMI | CTAG | 1 cut(s) | 35 |
| StyD4I | CCNGG | 1 cut(s) | 155 |
| StyI | CCWWGG | 1 cut(s) | 86 |
| TaaI | ACNGT | 1 cut(s) | 248 |
| TasI | AATT | 1 cut(s) | 211 |
| TfiI | GAWTC | 1 cut(s) | 224 |
| TscAI | CASTG | 1 cut(s) | 87 |
| TseFI | GTSAC | 2 cut(s) | 204, 248 |
| Tsp45I | GTSAC | 2 cut(s) | 204, 248 |
| TspDTI | ATGAA | 1 cut(s) | 51 |
| TspRI | CASTG | 1 cut(s) | 87 |
| VneI | GTGCAC | 1 cut(s) | 72 |
| XspI | CTAG | 1 cut(s) | 35 |