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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 353 |
| AciI | CCGC | 1 cut(s) | 338 |
| AclWI | GGATC | 2 cut(s) | 435, 460 |
| AcsI | RAATTY | 1 cut(s) | 61 |
| AdeI | CACNNNGTG | 1 cut(s) | 353 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 353 |
| AflIII | ACRYGT | 1 cut(s) | 168 |
| AgsI | TTSAA | 1 cut(s) | 29 |
| AluBI | AGCT | 1 cut(s) | 472 |
| AluI | AGCT | 1 cut(s) | 472 |
| Alw26I | GTCTC | 1 cut(s) | 483 |
| AlwI | GGATC | 2 cut(s) | 435, 460 |
| AoxI | GGCC | 1 cut(s) | 232 |
| ApoI | RAATTY | 1 cut(s) | 61 |
| BauI | CACGAG | 1 cut(s) | 264 |
| BccI | CCATC | 1 cut(s) | 393 |
| BcoDI | GTCTC | 1 cut(s) | 483 |
| BfaI | CTAG | 1 cut(s) | 128 |
| BfmI | CTRYAG | 1 cut(s) | 69 |
| BisI | GCNGC | 1 cut(s) | 339 |
| BlsI | GCNGC | 1 cut(s) | 340 |
| BmrI | ACTGGG | 1 cut(s) | 156 |
| BmsI | GCATC | 3 cut(s) | 14, 205, 405 |
| BmuI | ACTGGG | 1 cut(s) | 156 |
| BsaBI | GATNNNNATC | 1 cut(s) | 36 |
| BsaJI | CCNNGG | 1 cut(s) | 246 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 353 |
| Bse1I | ACTGG | 1 cut(s) | 151 |
| Bse8I | GATNNNNATC | 1 cut(s) | 36 |
| BseDI | CCNNGG | 1 cut(s) | 246 |
| BseGI | GGATG | 1 cut(s) | 461 |
| BseJI | GATNNNNATC | 1 cut(s) | 36 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 353 |
| BseMII | CTCAG | 1 cut(s) | 55 |
| BseNI | ACTGG | 1 cut(s) | 151 |
| BseRI | GAGGAG | 1 cut(s) | 377 |
| BsgI | GTGCAG | 1 cut(s) | 278 |
| BshFI | GGCC | 1 cut(s) | 234 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 353 |
| BsmAI | GTCTC | 1 cut(s) | 483 |
| BsnI | GGCC | 1 cut(s) | 234 |
| Bsp143I | GATC | 3 cut(s) | 31, 427, 452 |
| BspACI | CCGC | 1 cut(s) | 338 |
| BspANI | GGCC | 1 cut(s) | 234 |
| BspCNI | CTCAG | 1 cut(s) | 54 |
| BspPI | GGATC | 2 cut(s) | 435, 460 |
| BsrI | ACTGG | 1 cut(s) | 151 |
| BssECI | CCNNGG | 1 cut(s) | 246 |
| BssMI | GATC | 3 cut(s) | 31, 427, 452 |
| BssSI | CACGAG | 1 cut(s) | 264 |
| Bst2BI | CACGAG | 1 cut(s) | 264 |
| Bst4CI | ACNGT | 1 cut(s) | 393 |
| BstC8I | GCNNGC | 2 cut(s) | 183, 220 |
| BstDEI | CTNAG | 2 cut(s) | 41, 495 |
| BstF5I | GGATG | 1 cut(s) | 461 |
| BstKTI | GATC | 3 cut(s) | 34, 430, 455 |
| BstMAI | GTCTC | 1 cut(s) | 483 |
| BstMBI | GATC | 3 cut(s) | 31, 427, 452 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 316 |
| BstSFI | CTRYAG | 1 cut(s) | 69 |
| BsuRI | GGCC | 1 cut(s) | 234 |
| BtsCI | GGATG | 1 cut(s) | 461 |
| BtsIMutI | CAGTG | 3 cut(s) | 49, 144, 398 |
| Cac8I | GCNNGC | 2 cut(s) | 183, 220 |
| CviAII | CATG | 4 cut(s) | 330, 380, 424, 483 |
| CviJI | RGCY | 7 cut(s) | 154, 181, 234, 245, 310, 416, 472 |
| CviKI_1 | RGCY | 7 cut(s) | 154, 181, 234, 245, 310, 416, 472 |
| DdeI | CTNAG | 2 cut(s) | 41, 495 |
| DpnI | GATC | 3 cut(s) | 33, 429, 454 |
| DpnII | GATC | 3 cut(s) | 31, 427, 452 |
| DraIII | CACNNNGTG | 1 cut(s) | 353 |
| Eco147I | AGGCCT | 1 cut(s) | 234 |
| FaeI | CATG | 4 cut(s) | 333, 383, 427, 486 |
| FaiI | YATR | 7 cut(s) | 302, 321, 331, 381, 407, 425, 484 |
| FatI | CATG | 4 cut(s) | 329, 379, 423, 482 |
| Fnu4HI | GCNGC | 1 cut(s) | 339 |
| FokI | GGATG | 1 cut(s) | 448 |
| Fsp4HI | GCNGC | 1 cut(s) | 339 |
| FspBI | CTAG | 1 cut(s) | 128 |
| GluI | GCNGC | 1 cut(s) | 339 |
| HaeIII | GGCC | 1 cut(s) | 234 |
| Hin1II | CATG | 4 cut(s) | 333, 383, 427, 486 |
| HindIII | AAGCTT | 1 cut(s) | 470 |
| HinfI | GANTC | 2 cut(s) | 262, 458 |
| Hpy166II | GTNNAC | 1 cut(s) | 49 |
| Hpy188I | TCNGA | 4 cut(s) | 202, 250, 276, 457 |
| Hpy188III | TCNNGA | 1 cut(s) | 29 |
| Hpy8I | GTNNAC | 1 cut(s) | 49 |
| HpyAV | CCTTC | 3 cut(s) | 111, 271, 362 |
| HpyCH4III | ACNGT | 1 cut(s) | 393 |
| HpyCH4IV | ACGT | 1 cut(s) | 168 |
| HpyCH4V | TGCA | 7 cut(s) | 86, 218, 259, 319, 379, 396, 488 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 316 |
| HpyF3I | CTNAG | 2 cut(s) | 41, 495 |
| HpySE526I | ACGT | 1 cut(s) | 168 |
| Hsp92II | CATG | 4 cut(s) | 333, 383, 427, 486 |
| Kzo9I | GATC | 3 cut(s) | 31, 427, 452 |
| LpnPI | CCDG | 5 cut(s) | 132, 168, 195, 327, 389 |
| LweI | GCATC | 3 cut(s) | 14, 205, 405 |
| MaeI | CTAG | 1 cut(s) | 128 |
| MaeII | ACGT | 1 cut(s) | 168 |
| MalI | GATC | 3 cut(s) | 33, 429, 454 |
| MboI | GATC | 3 cut(s) | 31, 427, 452 |
| MboII | GAAGA | 1 cut(s) | 109 |
| MfeI | CAATTG | 1 cut(s) | 12 |
| MluCI | AATT | 2 cut(s) | 12, 61 |
| MlyI | GAGTC | 1 cut(s) | 256 |
| MmeI | TCCRAC | 1 cut(s) | 189 |
| MnlI | CCTC | 4 cut(s) | 135, 256, 355, 358 |
| MslI | CAYNNNNRTG | 1 cut(s) | 54 |
| MunI | CAATTG | 1 cut(s) | 12 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 316 |
| NdeII | GATC | 3 cut(s) | 31, 427, 452 |
| NlaIII | CATG | 4 cut(s) | 333, 383, 427, 486 |
| PceI | AGGCCT | 1 cut(s) | 234 |
| PfeI | GAWTC | 1 cut(s) | 458 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 353 |
| PkrI | GCNGC | 1 cut(s) | 340 |
| PleI | GAGTC | 1 cut(s) | 256 |
| PpsI | GAGTC | 1 cut(s) | 256 |
| RseI | CAYNNNNRTG | 1 cut(s) | 54 |
| SatI | GCNGC | 1 cut(s) | 339 |
| Sau3AI | GATC | 3 cut(s) | 31, 427, 452 |
| SchI | GAGTC | 1 cut(s) | 256 |
| SetI | ASST | 6 cut(s) | 127, 171, 316, 352, 373, 474 |
| SfaNI | GCATC | 3 cut(s) | 14, 205, 405 |
| SfcI | CTRYAG | 1 cut(s) | 69 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 54 |
| Sse9I | AATT | 2 cut(s) | 12, 61 |
| SseBI | AGGCCT | 1 cut(s) | 234 |
| SsiI | CCGC | 1 cut(s) | 338 |
| SspMI | CTAG | 1 cut(s) | 128 |
| StuI | AGGCCT | 1 cut(s) | 234 |
| TaaI | ACNGT | 1 cut(s) | 393 |
| TaiI | ACGT | 1 cut(s) | 171 |
| TaqI | TCGA | 2 cut(s) | 140, 173 |
| TasI | AATT | 2 cut(s) | 12, 61 |
| TauI | GCSGC | 1 cut(s) | 341 |
| TfiI | GAWTC | 1 cut(s) | 458 |
| TscAI | CASTG | 3 cut(s) | 49, 151, 398 |
| TspDTI | ATGAA | 1 cut(s) | 450 |
| TspRI | CASTG | 3 cut(s) | 49, 151, 398 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 353 |
| XapI | RAATTY | 1 cut(s) | 61 |
| XspI | CTAG | 1 cut(s) | 128 |