Rroxscaffold_6G00398970

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001257
Physical Location & Seq
Reverse (-)
20858276 .. 20861307
3032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00398970.1

Sequence Viewer

Length: 1023 bp
ATGGTCGAAGGCATCGCCGGCCTCCGGGAAGCTTATATCGGCGGCCACTTCGTCCTCAACACCGCTCTTGCCACCGCCCGAACACATGCACCGACGAGATCCGTAGACACTACGCCGGAGTCGCTAACGTCGGATCCGGATGGTCTCGTCGGACCGTATCCGCGACCAAACATCGAAACCGTCGCCGAATTCTTCTCCGCCCGAGGTGGTGGTGATGATATTACTCGATCGAACGACCTCGCCGCCGACGAAGCCGTCTTCCGCCATGGACGAGTACTTGGAATGCGCCACATCGATTCTCTCATAACTGAAAAAAACAAATTGAATTGGCTAAACAAAGCACAAATGGCATCAACAATTGGTCGATTGTTTCAAGATCAGAACCTCAGTGTTCACTCTAATGGAGATTCTGCTGTTGGAAAGGGTGGTGCAAAACAACAGAAGAAAGCAGGGTTTGGTGGAAGGAAACCTCTAGGAGATGTATCGAATACAGGGAAGCCTGATTTTACCAAGGTGTCGAAAAAGCCTGCTCTTACCAATGTTTCTGAAATCATTGCTGATGCAAGCAATAAGAAAGGCCTTTGTAAAGCCTCGGACAAAGTGCAGACTCGTGGCAGCCGCAAGGCACTATCTGATGTGTCTAACACAGTCAAACCACCTGTGCACAAGAAGTCAAGTGTTGTGGCACAACCACCTTGTGGTTTTGAGGAAGAAGGATTCTTGCATGACCATCAACAGTGCATCAAATCTATGAGAAAGGCTAACCAAATGGATCAAATGGATTTTTTTATGATAATACAAGGATCAGATTCATCCAAGAAGCTTCTATCTCCATGTGCAGTTTCTCAGTTTCGTAAAGTTGAGCCGGAGAGTTCCATGAGGTACTTGGATTTGAAAGAGATGCCTGAGCTGTCTATCAAGCTTGACTCTTCACCTCATTGCAAGTCTCCATTGTCACCAACTCATACCAAGTCCACTTGGATTTGGGATGATTGTGATTTTCAGGTGATGGAGACACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

340

Amino Acids

37.1

Weight (kDa)

8.77

Isoelectric Point (pI)

35.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 254
AccB7I CCANNNNNTGG 1 cut(s) 698
AccBSI CCGCTC 1 cut(s) 65
AccI GTMKAC 1 cut(s) 105
AccII CGCG 1 cut(s) 163
AccIII TCCGGA 1 cut(s) 136
AciI CCGC 8 cut(s) 42, 63, 75, 161, 198, 243, 262, 619
AclWI GGATC 5 cut(s) 93, 128, 141, 780, 811
AcoI YGGCCR 1 cut(s) 43
AcsI RAATTY 1 cut(s) 188
AdeI CACNNNGTG 1 cut(s) 698
AfaI GTAC 2 cut(s) 276, 884
AfiI CCNNNNNNNGG 2 cut(s) 24, 698
AgsI TTSAA 3 cut(s) 325, 374, 895
AluBI AGCT 4 cut(s) 32, 823, 910, 922
AluI AGCT 4 cut(s) 32, 823, 910, 922
Alw21I GWGCWC 1 cut(s) 666
Alw26I GTCTC 3 cut(s) 149, 951, 1007
Alw44I GTGCAC 1 cut(s) 662
AlwI GGATC 5 cut(s) 93, 128, 141, 780, 811
Ama87I CYCGRG 1 cut(s) 201
Aor13HI TCCGGA 1 cut(s) 136
AoxI GGCC 3 cut(s) 19, 43, 577
ApaLI GTGCAC 1 cut(s) 662
ApeKI GCWGC 1 cut(s) 615
ApoI RAATTY 1 cut(s) 188
AspLEI GCGC 1 cut(s) 288
AspS9I GGNCC 1 cut(s) 152
AsuC2I CCSGG 1 cut(s) 26
AsuHPI GGTGA 4 cut(s) 224, 924, 948, 1018
AvaI CYCGRG 1 cut(s) 201
AvaII GGWCC 1 cut(s) 152
BaeGI GKGCMC 1 cut(s) 666
BamHI GGATCC 1 cut(s) 133
BauI CACGAG 1 cut(s) 609
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 666
BbvI GCAGC 1 cut(s) 627
BccI CCATC 3 cut(s) 134, 738, 1003
BceAI ACGGC 1 cut(s) 239
BciVI GTATCC 1 cut(s) 168
BcnI CCSGG 1 cut(s) 26
BcoDI GTCTC 3 cut(s) 149, 951, 1007
BfaI CTAG 1 cut(s) 473
BfuI GTATCC 1 cut(s) 168
BisI GCNGC 4 cut(s) 43, 243, 616, 619
BlsI GCNGC 4 cut(s) 44, 244, 617, 620
BmcAI AGTACT 1 cut(s) 276
Bme1390I CCNGG 1 cut(s) 26
Bme18I GGWCC 1 cut(s) 152
BmeT110I CYCGRG 1 cut(s) 201
BmgT120I GGNCC 1 cut(s) 152
BmiI GGNNCC 1 cut(s) 135
BmrFI CCNGG 1 cut(s) 26
BmsI GCATC 5 cut(s) 21, 359, 550, 750, 891
BpiI GAAGAC 1 cut(s) 250
Bpu10I CCTNAGC 1 cut(s) 906
BpuMI CCSGG 1 cut(s) 26
Bsa29I ATCGAT 1 cut(s) 294
BsaI GGTCTC 1 cut(s) 149
BsaJI CCNNGG 4 cut(s) 202, 265, 510, 591
BsaWI WCCGGW 1 cut(s) 136
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 698
Bse118I RCCGGY 1 cut(s) 17
Bse3DI GCAATG 2 cut(s) 552, 937
BseAI TCCGGA 1 cut(s) 136
BseCI ATCGAT 1 cut(s) 294
BseDI CCNNGG 4 cut(s) 202, 265, 510, 591
BseGI GGATG 3 cut(s) 145, 812, 994
BseLI CCNNNNNNNGG 2 cut(s) 24, 698
BseMI GCAATG 2 cut(s) 552, 937
BseMII CTCAG 3 cut(s) 400, 860, 897
BseSI GKGCMC 1 cut(s) 666
BseXI GCAGC 1 cut(s) 627
BsgI GTGCAG 2 cut(s) 623, 858
Bsh1236I CGCG 1 cut(s) 163
Bsh1285I CGRYCG 1 cut(s) 230
BshFI GGCC 3 cut(s) 21, 45, 579
BshVI ATCGAT 1 cut(s) 294
BsiEI CGRYCG 1 cut(s) 230
BsiHKAI GWGCWC 1 cut(s) 666
BsiHKCI CYCGRG 1 cut(s) 201
BsiSI CCGG 5 cut(s) 18, 25, 116, 137, 866
BslI CCNNNNNNNGG 2 cut(s) 24, 698
BsmAI GTCTC 3 cut(s) 149, 951, 1007
BsmI GAATGC 1 cut(s) 288
BsnI GGCC 3 cut(s) 21, 45, 579
Bso31I GGTCTC 1 cut(s) 149
BsoBI CYCGRG 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 666
Bsp13I TCCGGA 1 cut(s) 136
Bsp143I GATC 6 cut(s) 98, 133, 227, 376, 772, 803
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 8 cut(s) 42, 63, 75, 161, 198, 243, 262, 619
BspANI GGCC 3 cut(s) 21, 45, 579
BspCNI CTCAG 3 cut(s) 399, 859, 898
BspDI ATCGAT 1 cut(s) 294
BspEI TCCGGA 1 cut(s) 136
BspFNI CGCG 1 cut(s) 163
BspLI GGNNCC 1 cut(s) 135
BspPI GGATC 5 cut(s) 93, 128, 141, 780, 811
BspTNI GGTCTC 1 cut(s) 149
BsrBI CCGCTC 1 cut(s) 65
BsrDI GCAATG 2 cut(s) 552, 937
BsrFI RCCGGY 1 cut(s) 17
BssAI RCCGGY 1 cut(s) 17
BssECI CCNNGG 4 cut(s) 202, 265, 510, 591
BssMI GATC 6 cut(s) 98, 133, 227, 376, 772, 803
BssSI CACGAG 1 cut(s) 609
BssT1I CCWWGG 2 cut(s) 265, 510
Bst2BI CACGAG 1 cut(s) 609
Bst4CI ACNGT 4 cut(s) 156, 181, 649, 738
Bst6I CTCTTC 1 cut(s) 934
BstC8I GCNNGC 3 cut(s) 19, 528, 565
BstDEI CTNAG 4 cut(s) 386, 846, 906, 1020
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 3 cut(s) 145, 812, 994
BstFNI CGCG 1 cut(s) 163
BstHHI GCGC 1 cut(s) 288
BstKTI GATC 6 cut(s) 101, 136, 230, 379, 775, 806
BstMAI GTCTC 3 cut(s) 149, 951, 1007
BstMBI GATC 6 cut(s) 98, 133, 227, 376, 772, 803
BstMCI CGRYCG 1 cut(s) 230
BstMWI GCNNNNNNNGC 4 cut(s) 18, 121, 251, 347
BstNSI RCATGY 1 cut(s) 89
BstSCI CCNGG 1 cut(s) 24
BstSLI GKGCMC 1 cut(s) 666
BstUI CGCG 1 cut(s) 163
BstV1I GCAGC 1 cut(s) 627
BstV2I GAAGAC 1 cut(s) 250
BstX2I RGATCY 2 cut(s) 98, 133
BstYI RGATCY 2 cut(s) 98, 133
Bsu15I ATCGAT 1 cut(s) 294
BsuI GTATCC 1 cut(s) 168
BsuRI GGCC 3 cut(s) 21, 45, 579
BsuTUI ATCGAT 1 cut(s) 294
BtgI CCRYGG 1 cut(s) 265
BtsCI GGATG 3 cut(s) 145, 812, 994
BtsIMutI CAGTG 2 cut(s) 394, 743
Cac8I GCNNGC 3 cut(s) 19, 528, 565
CfoI GCGC 1 cut(s) 288
Cfr10I RCCGGY 1 cut(s) 17
Cfr13I GGNCC 1 cut(s) 152
ClaI ATCGAT 1 cut(s) 294
CpoI CGGWCCG 1 cut(s) 152
Csp6I GTAC 2 cut(s) 275, 883
CspCI CAANNNNNGTGG 2 cut(s) 663, 698
CspI CGGWCCG 1 cut(s) 152
CviAII CATG 5 cut(s) 86, 266, 725, 834, 877
CviQI GTAC 2 cut(s) 275, 883
DdeI CTNAG 4 cut(s) 386, 846, 906, 1020
DpnI GATC 6 cut(s) 100, 135, 229, 378, 774, 805
DpnII GATC 6 cut(s) 98, 133, 227, 376, 772, 803
DraIII CACNNNGTG 1 cut(s) 698
DrdI GACNNNNNNGTC 1 cut(s) 254
DseDI GACNNNNNNGTC 1 cut(s) 254
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 1 cut(s) 934
EarI CTCTTC 1 cut(s) 934
EciI GGCGGA 2 cut(s) 187, 251
Eco130I CCWWGG 2 cut(s) 265, 510
Eco147I AGGCCT 1 cut(s) 579
Eco31I GGTCTC 1 cut(s) 149
Eco47I GGWCC 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 201
EcoRI GAATTC 1 cut(s) 188
EcoT14I CCWWGG 2 cut(s) 265, 510
ErhI CCWWGG 2 cut(s) 265, 510
FaeI CATG 5 cut(s) 89, 269, 728, 837, 880
FatI CATG 5 cut(s) 85, 265, 724, 833, 876
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 4 cut(s) 43, 243, 616, 619
FokI GGATG 3 cut(s) 152, 799, 1001
Fsp4HI GCNGC 4 cut(s) 43, 243, 616, 619
FspBI CTAG 1 cut(s) 473
GlaI GCGC 1 cut(s) 287
GluI GCNGC 4 cut(s) 43, 243, 616, 619
HaeIII GGCC 3 cut(s) 21, 45, 579
HapII CCGG 5 cut(s) 18, 25, 116, 137, 866
HhaI GCGC 1 cut(s) 288
Hin1II CATG 5 cut(s) 89, 269, 728, 837, 880
Hin6I GCGC 1 cut(s) 286
HinP1I GCGC 1 cut(s) 286
HindIII AAGCTT 3 cut(s) 30, 821, 920
HinfI GANTC 7 cut(s) 119, 296, 407, 607, 717, 809, 926
HpaII CCGG 5 cut(s) 18, 25, 116, 137, 866
HphI GGTGA 4 cut(s) 224, 924, 948, 1018
Hpy166II GTNNAC 4 cut(s) 106, 394, 664, 975
Hpy188I TCNGA 7 cut(s) 133, 152, 381, 547, 595, 634, 808
Hpy188III TCNNGA 2 cut(s) 137, 374
Hpy8I GTNNAC 4 cut(s) 106, 394, 664, 975
Hpy99I CGWCG 5 cut(s) 97, 133, 152, 185, 251
HpyAV CCTTC 2 cut(s) 456, 707
HpyCH4III ACNGT 4 cut(s) 156, 181, 649, 738
HpyCH4IV ACGT 1 cut(s) 128
HpyCH4V TGCA 9 cut(s) 89, 431, 563, 604, 664, 724, 741, 839, 942
HpyF10VI GCNNNNNNNGC 4 cut(s) 18, 121, 251, 347
HpyF3I CTNAG 4 cut(s) 386, 846, 906, 1020
HpySE526I ACGT 1 cut(s) 128
Hsp92II CATG 5 cut(s) 89, 269, 728, 837, 880
HspAI GCGC 1 cut(s) 286
Kpn2I TCCGGA 1 cut(s) 136
KroI GCCGGC 1 cut(s) 17
KroNI GCCGGC 1 cut(s) 19
Kzo9I GATC 6 cut(s) 98, 133, 227, 376, 772, 803
Lsp1109I GCAGC 1 cut(s) 627
LweI GCATC 5 cut(s) 21, 359, 550, 750, 891
MaeI CTAG 1 cut(s) 473
MaeII ACGT 1 cut(s) 128
MaeIII GTNAC 1 cut(s) 954
MalI GATC 6 cut(s) 100, 135, 229, 378, 774, 805
MbiI CCGCTC 1 cut(s) 65
MboI GATC 6 cut(s) 98, 133, 227, 376, 772, 803
MboII GAAGA 5 cut(s) 184, 250, 454, 722, 921
MfeI CAATTG 1 cut(s) 357
MflI RGATCY 2 cut(s) 98, 133
MhlI GDGCHC 1 cut(s) 666
MluCI AATT 4 cut(s) 188, 320, 325, 357
MlyI GAGTC 3 cut(s) 128, 601, 920
MmeI TCCRAC 3 cut(s) 111, 130, 397
MroI TCCGGA 1 cut(s) 136
MroNI GCCGGC 1 cut(s) 17
MslI CAYNNNNRTG 1 cut(s) 399
MspI CCGG 5 cut(s) 18, 25, 116, 137, 866
MspR9I CCNGG 1 cut(s) 26
MunI CAATTG 1 cut(s) 357
Mva1269I GAATGC 1 cut(s) 288
MvnI CGCG 1 cut(s) 163
MwoI GCNNNNNNNGC 4 cut(s) 18, 121, 251, 347
NaeI GCCGGC 1 cut(s) 19
NciI CCSGG 1 cut(s) 26
NcoI CCATGG 1 cut(s) 265
NdeII GATC 6 cut(s) 98, 133, 227, 376, 772, 803
NgoMIV GCCGGC 1 cut(s) 17
NlaIII CATG 5 cut(s) 89, 269, 728, 837, 880
NlaIV GGNNCC 1 cut(s) 135
NmuCI GTSAC 1 cut(s) 954
NspI RCATGY 1 cut(s) 89
PceI AGGCCT 1 cut(s) 579
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PctI GAATGC 1 cut(s) 288
PdiI GCCGGC 1 cut(s) 19
PfeI GAWTC 4 cut(s) 296, 407, 717, 809
PflMI CCANNNNNTGG 1 cut(s) 698
PfoI TCCNGGA 1 cut(s) 24
PkrI GCNGC 4 cut(s) 44, 244, 617, 620
Ple19I CGATCG 1 cut(s) 230
PleI GAGTC 3 cut(s) 127, 601, 920
PpsI GAGTC 3 cut(s) 127, 601, 920
PspN4I GGNNCC 1 cut(s) 135
PspPI GGNCC 1 cut(s) 152
PsuI RGATCY 2 cut(s) 98, 133
PvuI CGATCG 1 cut(s) 230
RsaI GTAC 2 cut(s) 276, 884
RsaNI GTAC 2 cut(s) 275, 883
RseI CAYNNNNRTG 1 cut(s) 399
Rsr2I CGGWCCG 1 cut(s) 152
RsrII CGGWCCG 1 cut(s) 152
SatI GCNGC 4 cut(s) 43, 243, 616, 619
Sau3AI GATC 6 cut(s) 98, 133, 227, 376, 772, 803
Sau96I GGNCC 1 cut(s) 152
ScaI AGTACT 1 cut(s) 276
SchI GAGTC 3 cut(s) 128, 601, 920
ScrFI CCNGG 1 cut(s) 26
SduI GDGCHC 1 cut(s) 666
SfaNI GCATC 5 cut(s) 21, 359, 550, 750, 891
SinI GGWCC 1 cut(s) 152
SmiMI CAYNNNNRTG 1 cut(s) 399
Sse9I AATT 4 cut(s) 188, 320, 325, 357
SseBI AGGCCT 1 cut(s) 579
SsiI CCGC 8 cut(s) 42, 63, 75, 161, 198, 243, 262, 619
SspMI CTAG 1 cut(s) 473
StuI AGGCCT 1 cut(s) 579
StyD4I CCNGG 1 cut(s) 24
StyI CCWWGG 2 cut(s) 265, 510
TaaI ACNGT 4 cut(s) 156, 181, 649, 738
TaiI ACGT 1 cut(s) 131
TaqI TCGA 8 cut(s) 6, 174, 226, 230, 294, 364, 485, 518
TasI AATT 4 cut(s) 188, 320, 325, 357
TatI WGTACW 1 cut(s) 274
TauI GCSGC 3 cut(s) 45, 245, 621
TfiI GAWTC 4 cut(s) 296, 407, 717, 809
TscAI CASTG 2 cut(s) 394, 743
TseFI GTSAC 1 cut(s) 954
TseI GCWGC 1 cut(s) 615
Tsp45I GTSAC 1 cut(s) 954
TspDTI ATGAA 1 cut(s) 801
TspGWI ACGGA 1 cut(s) 91
TspRI CASTG 2 cut(s) 394, 743
Van91I CCANNNNNTGG 1 cut(s) 698
VneI GTGCAC 1 cut(s) 662
VpaK11BI GGWCC 1 cut(s) 152
XapI RAATTY 1 cut(s) 188
XceI RCATGY 1 cut(s) 89
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XmiI GTMKAC 1 cut(s) 105
XspI CTAG 1 cut(s) 473
ZrmI AGTACT 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.