MD13G1123100.v1.1

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001652
Physical Location & Seq
Reverse (-)
9128856 .. 9131629
2774 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1123100.v1.1.491

Sequence Viewer

Length: 780 bp
ATGGCGGCTCCGTTGACCACCCTGTCAAATGTTGGTTCGCCTCCGGCTTCTCGCAGCGTGTCTCAGATCAAAGTTCTGACCCCTACCTGTTCTTCCGTCACAGTCCCATCGTCGTCTAACAAAACGGCGTCGTTGTCCTCCAGCTTCTTCTCCAGGTCGTCTCGTTTGTCCCCTCTGACATTCTCTTCGAACCCTAGTTCCGGGAAAAGGACCAGTCTTGCTAGCTCAGTCCGCGCCAGTGCTGAGGAAGCAACTTTACAATCAAAAGTGACCCATAAGGTGTATTTTGATATTAGCATTGGGAACCCAGTTGGGAAGCTTGCTGGAAGGATTGTGATTGGATTGTATGGCGATGATGTGCCCCAAACCGTGGAGAATTTCCGTGCTCTTTGCACAGGTGATAAAGGCTTTGGATTCAAGGGTTCCGCGTTCCATCGTGTGATCAAGGATTTCATGATTCAAGGAGGGGACTTTGACAAAGGCAATGGCACTGGAGGTAAAAGCATTTACGGACGTACTTTCAAGGATGAGAACTTCAAGTTGTCTCATACTGGCCCCGGAGTTGTTAGCATGGCCAATGCAGGGCCCAATACCAATGGTAGCCAGTTCTTCATATGCACGGTCAAGACACCATGGCTCGACAACAGGCATGTCGTTTTTGGGCAAGTGGTGGAAGGCCTGGATGTTGTTAAACTAATTGAGTCTCAAGAGACAGACAGAGGTGATCGCCCTACAAAGAAGGTGATCATCGCGGACTGTGGAGAGCTGCCAGTGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pro_isomerase

Protein Analysis

260

Amino Acids

27.43

Weight (kDa)

9.41

Isoelectric Point (pI)

36.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_isomerase PF00160 102 - 254 5.1e-51 Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 22
AccB7I CCANNNNNTGG 1 cut(s) 370
AccII CGCG 3 cut(s) 234, 428, 752
AciI CCGC 4 cut(s) 5, 232, 426, 752
AcoI YGGCCR 1 cut(s) 573
AcsI RAATTY 1 cut(s) 376
AcyI GRCGYC 1 cut(s) 128
AfaI GTAC 1 cut(s) 517
AfiI CCNNNNNNNGG 4 cut(s) 200, 207, 370, 582
AgsI TTSAA 4 cut(s) 418, 461, 523, 538
AjnI CCWGG 2 cut(s) 152, 678
AloI GAACNNNNNNTCC 2 cut(s) 182, 214
AluBI AGCT 4 cut(s) 144, 225, 319, 766
AluI AGCT 4 cut(s) 144, 225, 319, 766
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 5 cut(s) 66, 165, 549, 704, 708
AoxI GGCC 5 cut(s) 553, 573, 584, 676, 774
ApaI GGGCCC 1 cut(s) 588
ApeKI GCWGC 2 cut(s) 54, 766
ApoI RAATTY 1 cut(s) 376
ArsI GACNNNNNNTTYG 2 cut(s) 169, 201
AspLEI GCGC 1 cut(s) 236
AspS9I GGNCC 4 cut(s) 210, 554, 584, 585
AsuC2I CCSGG 2 cut(s) 202, 558
AsuHPI GGTGA 3 cut(s) 410, 734, 754
AsuII TTCGAA 1 cut(s) 188
AsuNHI GCTAGC 1 cut(s) 221
AvaII GGWCC 1 cut(s) 210
BaeGI GKGCMC 2 cut(s) 363, 588
BalI TGGCCA 1 cut(s) 575
BanII GRGCYC 1 cut(s) 588
BarI GAAGNNNNNNTAC 2 cut(s) 240, 272
Bbv12I GWGCWC 1 cut(s) 388
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 2 cut(s) 66, 753
BccI CCATC 2 cut(s) 115, 441
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 2 cut(s) 154, 680
BclI TGATCA 2 cut(s) 441, 744
BcnI CCSGG 2 cut(s) 202, 558
BcoDI GTCTC 5 cut(s) 66, 165, 549, 704, 708
BfaI CTAG 2 cut(s) 195, 222
BisI GCNGC 3 cut(s) 6, 55, 767
BlsI GCNGC 3 cut(s) 7, 56, 768
Bme1390I CCNGG 4 cut(s) 154, 202, 558, 680
Bme18I GGWCC 1 cut(s) 210
BmgT120I GGNCC 4 cut(s) 210, 554, 584, 585
BmiI GGNNCC 5 cut(s) 9, 305, 424, 556, 586
BmrFI CCNGG 4 cut(s) 154, 202, 558, 680
BmrI ACTGGG 1 cut(s) 302
BmtI GCTAGC 1 cut(s) 225
BmuI ACTGGG 1 cut(s) 302
BpmI CTGGAG 3 cut(s) 124, 136, 513
Bpu10I CCTNAGC 1 cut(s) 243
Bpu14I TTCGAA 1 cut(s) 188
BpuEI CTTGAG 1 cut(s) 690
BpuMI CCSGG 2 cut(s) 202, 558
BsaHI GRCGYC 1 cut(s) 128
BsaJI CCNNGG 3 cut(s) 369, 556, 632
Bsc4I CCNNNNNNNGG 4 cut(s) 200, 207, 370, 582
Bse1I ACTGG 7 cut(s) 213, 237, 308, 496, 556, 604, 770
Bse3DI GCAATG 1 cut(s) 490
BseBI CCWGG 2 cut(s) 154, 680
BseDI CCNNGG 3 cut(s) 369, 556, 632
BseGI GGATG 2 cut(s) 532, 688
BseLI CCNNNNNNNGG 4 cut(s) 200, 207, 370, 582
BseMI GCAATG 1 cut(s) 490
BseMII CTCAG 3 cut(s) 77, 234, 240
BseNI ACTGG 7 cut(s) 213, 237, 308, 496, 556, 604, 770
BseSI GKGCMC 2 cut(s) 363, 588
BseXI GCAGC 2 cut(s) 66, 753
Bsh1236I CGCG 3 cut(s) 234, 428, 752
BshFI GGCC 5 cut(s) 555, 575, 586, 678, 776
BsiHKAI GWGCWC 1 cut(s) 388
BsiSI CCGG 3 cut(s) 44, 201, 558
BslFI GGGAC 3 cut(s) 89, 154, 482
BslI CCNNNNNNNGG 4 cut(s) 200, 207, 370, 582
BsmAI GTCTC 5 cut(s) 66, 165, 549, 704, 708
BsmBI CGTCTC 1 cut(s) 165
BsmFI GGGAC 3 cut(s) 89, 154, 482
BsnI GGCC 5 cut(s) 555, 575, 586, 678, 776
Bsp119I TTCGAA 1 cut(s) 188
Bsp120I GGGCCC 1 cut(s) 584
Bsp1286I GDGCHC 3 cut(s) 363, 388, 588
Bsp143I GATC 4 cut(s) 66, 441, 724, 744
Bsp19I CCATGG 1 cut(s) 632
BspACI CCGC 4 cut(s) 5, 232, 426, 752
BspANI GGCC 5 cut(s) 555, 575, 586, 678, 776
BspCNI CTCAG 3 cut(s) 76, 235, 239
BspFNI CGCG 3 cut(s) 234, 428, 752
BspHI TCATGA 1 cut(s) 453
BspLI GGNNCC 5 cut(s) 9, 305, 424, 556, 586
BspOI GCTAGC 1 cut(s) 225
BspT104I TTCGAA 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 490
BsrI ACTGG 7 cut(s) 213, 237, 308, 496, 556, 604, 770
BssECI CCNNGG 3 cut(s) 369, 556, 632
BssMI GATC 4 cut(s) 66, 441, 724, 744
BssNI GRCGYC 1 cut(s) 128
BssT1I CCWWGG 1 cut(s) 632
Bst2UI CCWGG 2 cut(s) 154, 680
Bst4CI ACNGT 4 cut(s) 103, 370, 622, 758
Bst6I CTCTTC 1 cut(s) 190
BstACI GRCGYC 1 cut(s) 128
BstBI TTCGAA 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 223, 321
BstDEI CTNAG 3 cut(s) 63, 226, 243
BstDSI CCRYGG 2 cut(s) 369, 632
BstF5I GGATG 2 cut(s) 532, 688
BstFNI CGCG 3 cut(s) 234, 428, 752
BstHHI GCGC 1 cut(s) 236
BstKTI GATC 4 cut(s) 69, 444, 727, 747
BstMAI GTCTC 5 cut(s) 66, 165, 549, 704, 708
BstMBI GATC 4 cut(s) 66, 441, 724, 744
BstMWI GCNNNNNNNGC 2 cut(s) 231, 248
BstNI CCWGG 2 cut(s) 154, 680
BstNSI RCATGY 1 cut(s) 653
BstSCI CCNGG 4 cut(s) 152, 200, 556, 678
BstSLI GKGCMC 2 cut(s) 363, 588
BstUI CGCG 3 cut(s) 234, 428, 752
BstV1I GCAGC 2 cut(s) 66, 753
BsuRI GGCC 5 cut(s) 555, 575, 586, 678, 776
BtgI CCRYGG 2 cut(s) 369, 632
BtgZI GCGATG 2 cut(s) 366, 733
BtsCI GGATG 2 cut(s) 532, 688
BtsIMutI CAGTG 3 cut(s) 244, 489, 777
Cac8I GCNNGC 2 cut(s) 223, 321
CciI TCATGA 1 cut(s) 453
CfoI GCGC 1 cut(s) 236
Cfr13I GGNCC 4 cut(s) 210, 554, 584, 585
CseI GACGC 1 cut(s) 117
Csp6I GTAC 1 cut(s) 516
CviAII CATG 4 cut(s) 454, 571, 633, 650
CviQI GTAC 1 cut(s) 516
DdeI CTNAG 3 cut(s) 63, 226, 243
DpnI GATC 4 cut(s) 68, 443, 726, 746
DpnII GATC 4 cut(s) 66, 441, 724, 744
DrdI GACNNNNNNGTC 1 cut(s) 22
DseDI GACNNNNNNGTC 1 cut(s) 22
EaeI YGGCCR 1 cut(s) 573
Eam1104I CTCTTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 632
Eco147I AGGCCT 1 cut(s) 678
Eco24I GRGCYC 1 cut(s) 588
Eco47I GGWCC 1 cut(s) 210
EcoO109I RGGNCCY 1 cut(s) 584
EcoRII CCWGG 2 cut(s) 152, 678
EcoT14I CCWWGG 1 cut(s) 632
EcoT38I GRGCYC 1 cut(s) 588
ErhI CCWWGG 1 cut(s) 632
Esp3I CGTCTC 1 cut(s) 165
FaeI CATG 4 cut(s) 457, 574, 636, 653
FaiI YATR 9 cut(s) 276, 348, 455, 549, 572, 614, 616, 634, 651
FaqI GGGAC 3 cut(s) 89, 154, 482
FatI CATG 4 cut(s) 453, 570, 632, 649
FauNDI CATATG 1 cut(s) 614
FbaI TGATCA 2 cut(s) 441, 744
Fnu4HI GCNGC 3 cut(s) 6, 55, 767
FokI GGATG 2 cut(s) 539, 695
FriOI GRGCYC 1 cut(s) 588
Fsp4HI GCNGC 3 cut(s) 6, 55, 767
FspBI CTAG 2 cut(s) 195, 222
GlaI GCGC 1 cut(s) 235
GluI GCNGC 3 cut(s) 6, 55, 767
GsuI CTGGAG 3 cut(s) 124, 136, 513
HaeIII GGCC 5 cut(s) 555, 575, 586, 678, 776
HapII CCGG 3 cut(s) 44, 201, 558
HgaI GACGC 1 cut(s) 117
HhaI GCGC 1 cut(s) 236
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 4 cut(s) 457, 574, 636, 653
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HindIII AAGCTT 1 cut(s) 317
HinfI GANTC 3 cut(s) 414, 457, 701
HpaII CCGG 3 cut(s) 44, 201, 558
HphI GGTGA 3 cut(s) 410, 734, 754
Hpy166II GTNNAC 1 cut(s) 15
Hpy188I TCNGA 3 cut(s) 66, 78, 177
Hpy188III TCNNGA 3 cut(s) 454, 625, 707
Hpy8I GTNNAC 1 cut(s) 15
Hpy99I CGWCG 2 cut(s) 115, 133
HpyAV CCTTC 3 cut(s) 321, 668, 733
HpyCH4III ACNGT 4 cut(s) 103, 370, 622, 758
HpyCH4IV ACGT 1 cut(s) 514
HpyCH4V TGCA 3 cut(s) 393, 581, 618
HpyF10VI GCNNNNNNNGC 2 cut(s) 231, 248
HpyF3I CTNAG 3 cut(s) 63, 226, 243
HpySE526I ACGT 1 cut(s) 514
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 4 cut(s) 457, 574, 636, 653
HspAI GCGC 1 cut(s) 234
Ksp22I TGATCA 2 cut(s) 441, 744
Kzo9I GATC 4 cut(s) 66, 441, 724, 744
LmnI GCTCC 1 cut(s) 13
Lsp1109I GCAGC 2 cut(s) 66, 753
MaeI CTAG 2 cut(s) 195, 222
MaeII ACGT 1 cut(s) 514
MaeIII GTNAC 2 cut(s) 97, 268
MalI GATC 4 cut(s) 68, 443, 726, 746
MboI GATC 4 cut(s) 66, 441, 724, 744
MboII GAAGA 4 cut(s) 84, 139, 177, 601
MhlI GDGCHC 3 cut(s) 363, 388, 588
MlsI TGGCCA 1 cut(s) 575
MluCI AATT 2 cut(s) 376, 696
MluNI TGGCCA 1 cut(s) 575
MlyI GAGTC 1 cut(s) 710
MnlI CCTC 7 cut(s) 51, 148, 183, 238, 458, 488, 713
Mox20I TGGCCA 1 cut(s) 575
MscI TGGCCA 1 cut(s) 575
MseI TTAA 1 cut(s) 690
Msp20I TGGCCA 1 cut(s) 575
MspI CCGG 3 cut(s) 44, 201, 558
MspR9I CCNGG 4 cut(s) 154, 202, 558, 680
MvaI CCWGG 2 cut(s) 154, 680
MvnI CGCG 3 cut(s) 234, 428, 752
MwoI GCNNNNNNNGC 2 cut(s) 231, 248
NciI CCSGG 2 cut(s) 202, 558
NcoI CCATGG 1 cut(s) 632
NdeI CATATG 1 cut(s) 614
NdeII GATC 4 cut(s) 66, 441, 724, 744
NheI GCTAGC 1 cut(s) 221
NlaIII CATG 4 cut(s) 457, 574, 636, 653
NlaIV GGNNCC 5 cut(s) 9, 305, 424, 556, 586
NmuCI GTSAC 2 cut(s) 97, 268
NspI RCATGY 1 cut(s) 653
NspV TTCGAA 1 cut(s) 188
PagI TCATGA 1 cut(s) 453
PceI AGGCCT 1 cut(s) 678
PfeI GAWTC 2 cut(s) 414, 457
PflMI CCANNNNNTGG 1 cut(s) 370
PfoI TCCNGGA 1 cut(s) 200
PkrI GCNGC 3 cut(s) 7, 56, 768
PleI GAGTC 1 cut(s) 709
PpsI GAGTC 1 cut(s) 709
Psp6I CCWGG 2 cut(s) 152, 678
PspGI CCWGG 2 cut(s) 152, 678
PspN4I GGNNCC 5 cut(s) 9, 305, 424, 556, 586
PspOMI GGGCCC 1 cut(s) 584
PspPI GGNCC 4 cut(s) 210, 554, 584, 585
RsaI GTAC 1 cut(s) 517
RsaNI GTAC 1 cut(s) 516
SaqAI TTAA 1 cut(s) 690
SatI GCNGC 3 cut(s) 6, 55, 767
Sau3AI GATC 4 cut(s) 66, 441, 724, 744
Sau96I GGNCC 4 cut(s) 210, 554, 584, 585
SchI GAGTC 1 cut(s) 710
ScrFI CCNGG 4 cut(s) 154, 202, 558, 680
SduI GDGCHC 3 cut(s) 363, 388, 588
SfuI TTCGAA 1 cut(s) 188
SinI GGWCC 1 cut(s) 210
SmlI CTYRAG 1 cut(s) 705
SmoI CTYRAG 1 cut(s) 705
Sse9I AATT 2 cut(s) 376, 696
SseBI AGGCCT 1 cut(s) 678
SsiI CCGC 4 cut(s) 5, 232, 426, 752
SspMI CTAG 2 cut(s) 195, 222
StuI AGGCCT 1 cut(s) 678
StyD4I CCNGG 4 cut(s) 152, 200, 556, 678
StyI CCWWGG 1 cut(s) 632
TaaI ACNGT 4 cut(s) 103, 370, 622, 758
TaiI ACGT 1 cut(s) 517
TaqI TCGA 2 cut(s) 188, 639
TasI AATT 2 cut(s) 376, 696
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 414, 457
Tru1I TTAA 1 cut(s) 690
Tru9I TTAA 1 cut(s) 690
TscAI CASTG 3 cut(s) 244, 496, 777
TseFI GTSAC 2 cut(s) 97, 268
TseI GCWGC 2 cut(s) 54, 766
Tsp45I GTSAC 2 cut(s) 97, 268
TspDTI ATGAA 2 cut(s) 442, 601
TspGWI ACGGA 3 cut(s) 85, 371, 525
TspRI CASTG 3 cut(s) 244, 496, 777
Van91I CCANNNNNTGG 1 cut(s) 370
VpaK11BI GGWCC 1 cut(s) 210
XapI RAATTY 1 cut(s) 376
XceI RCATGY 1 cut(s) 653
XspI CTAG 2 cut(s) 195, 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.