Rw7G028310

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001652
Physical Location & Seq
Reverse (-)
38479340 .. 38484263
4924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G028310.1

Sequence Viewer

Length: 696 bp
ATGTCTCCAATTACACAACTCACAAATCCAAACAAACAAAACCGAAACAAAGATTGTAATTCTTGTTCCCGCTCATCCCGCTCACTCCATCCGGTCCCGACAACGAAGTCGACCATAGCAATCTCTCAAACTCTCAGAATCTCATCCCTCTCTCTTCGACTCTCAGACTCACTCTCCCCCAATCTCCTCCTGTTTGCTCAATTTGACTTTCAAACCTTTGTGTTTTTTTTTCTTTTCTTTTTTCTTTTTGCTTTCACTTCTGTTTACAGCAAATTTGCTTTCACTGTGGAGAATTTCCGTTCCCTTTGCACAGGTGAGAAAGGCTTTGGGTACAAGGGTTCTGCCTTCCATCGTGTGATCAAGGATTTCATGATTCAAGGAGGGGACTTCGATAAGGGCAATGGTACTGGAGGCAAAAGCATTTATGGGCATACATTTAAAGATGAGAACTTTAAGTTGCTTCATACTGGACTAGGAGTTCTTAGCATGGCAAATGCAGGCCCCAATACCAATGGAAGTCAGTTCTTCATATGCACAGTACAGACGCCATGGCTAAATGGGAGGCACGTTGTGTTTGGGCAAGTTTTGGAAGGCATGGAAATTGTTAGGCTGATCGAGTCGCAGGAGACAGATAGAGGTGACCGTCCTACAAAGAAGGTAGTCATTGCTGACTGCGGAGAGCTTCCAGTAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pro_isomerase

Protein Analysis

231

Amino Acids

25.68

Weight (kDa)

9.16

Isoelectric Point (pI)

33.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_isomerase PF00160 94 - 226 4.2e-44 Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 106
AccBSI CCGCTC 2 cut(s) 72, 81
AccI GTMKAC 1 cut(s) 110
AciI CCGC 3 cut(s) 70, 79, 675
AcsI RAATTY 2 cut(s) 272, 292
AcyI GRCGYC 1 cut(s) 545
AdeI CACNNNGTG 1 cut(s) 571
AfaI GTAC 3 cut(s) 332, 406, 540
AgsI TTSAA 2 cut(s) 212, 377
AloI GAACNNNNNNTCC 2 cut(s) 462, 494
AluBI AGCT 1 cut(s) 682
AluI AGCT 1 cut(s) 682
Alw26I GTCTC 2 cut(s) 9, 620
AoxI GGCC 1 cut(s) 499
ApoI RAATTY 2 cut(s) 272, 292
AspS9I GGNCC 2 cut(s) 94, 500
AsuHPI GGTGA 2 cut(s) 326, 650
AvaII GGWCC 1 cut(s) 94
BaeI ACNNNNGTAYC 2 cut(s) 322, 355
BccI CCATC 2 cut(s) 96, 357
BclI TGATCA 1 cut(s) 357
BcoDI GTCTC 2 cut(s) 9, 620
BfaI CTAG 1 cut(s) 473
Bme18I GGWCC 1 cut(s) 94
BmgT120I GGNCC 2 cut(s) 94, 500
BmiI GGNNCC 2 cut(s) 96, 502
BpmI CTGGAG 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 545
BsaJI CCNNGG 1 cut(s) 548
BsaWI WCCGGW 1 cut(s) 91
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bse1I ACTGG 3 cut(s) 412, 472, 686
Bse3DI GCAATG 2 cut(s) 406, 663
BseDI CCNNGG 1 cut(s) 548
BseGI GGATG 3 cut(s) 74, 88, 143
BseMI GCAATG 2 cut(s) 406, 663
BseMII CTCAG 2 cut(s) 148, 177
BseNI ACTGG 3 cut(s) 412, 472, 686
BseRI GAGGAG 1 cut(s) 176
BshFI GGCC 1 cut(s) 501
BsiSI CCGG 1 cut(s) 92
BslFI GGGAC 2 cut(s) 80, 398
BsmAI GTCTC 2 cut(s) 9, 620
BsmFI GGGAC 2 cut(s) 80, 398
BsnI GGCC 1 cut(s) 501
Bsp143I GATC 2 cut(s) 357, 612
Bsp19I CCATGG 1 cut(s) 548
BspACI CCGC 3 cut(s) 70, 79, 675
BspANI GGCC 1 cut(s) 501
BspCNI CTCAG 2 cut(s) 147, 176
BspHI TCATGA 1 cut(s) 369
BspLI GGNNCC 2 cut(s) 96, 502
BsrBI CCGCTC 2 cut(s) 72, 81
BsrDI GCAATG 2 cut(s) 406, 663
BsrI ACTGG 3 cut(s) 412, 472, 686
BssECI CCNNGG 1 cut(s) 548
BssMI GATC 2 cut(s) 357, 612
BssNI GRCGYC 1 cut(s) 545
BssT1I CCWWGG 1 cut(s) 548
Bst4CI ACNGT 3 cut(s) 286, 538, 644
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 1 cut(s) 545
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 3 cut(s) 134, 163, 482
BstDSI CCRYGG 1 cut(s) 548
BstEII GGTNACC 1 cut(s) 638
BstF5I GGATG 3 cut(s) 74, 88, 143
BstKTI GATC 2 cut(s) 360, 615
BstMAI GTCTC 2 cut(s) 9, 620
BstMBI GATC 2 cut(s) 357, 612
BstMWI GCNNNNNNNGC 1 cut(s) 78
BstPI GGTNACC 1 cut(s) 638
BsuRI GGCC 1 cut(s) 501
BtgI CCRYGG 1 cut(s) 548
BtsCI GGATG 3 cut(s) 74, 88, 143
BtsIMutI CAGTG 1 cut(s) 282
Cac8I GCNNGC 1 cut(s) 499
CciI TCATGA 1 cut(s) 369
Cfr13I GGNCC 2 cut(s) 94, 500
CseI GACGC 1 cut(s) 553
Csp6I GTAC 3 cut(s) 331, 405, 539
CviAII CATG 4 cut(s) 370, 487, 549, 595
CviJI RGCY 6 cut(s) 324, 501, 553, 610, 682, 692
CviKI_1 RGCY 6 cut(s) 324, 501, 553, 610, 682, 692
CviQI GTAC 3 cut(s) 331, 405, 539
DdeI CTNAG 3 cut(s) 134, 163, 482
DpnI GATC 2 cut(s) 359, 614
DpnII GATC 2 cut(s) 357, 612
DraI TTTAAA 1 cut(s) 439
DraIII CACNNNGTG 1 cut(s) 571
DrdI GACNNNNNNGTC 1 cut(s) 106
DseDI GACNNNNNNGTC 1 cut(s) 106
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 548
Eco47I GGWCC 1 cut(s) 94
Eco91I GGTNACC 1 cut(s) 638
EcoO109I RGGNCCY 1 cut(s) 500
EcoO65I GGTNACC 1 cut(s) 638
EcoT14I CCWWGG 1 cut(s) 548
ErhI CCWWGG 1 cut(s) 548
FaeI CATG 4 cut(s) 373, 490, 552, 598
FaqI GGGAC 2 cut(s) 80, 398
FatI CATG 4 cut(s) 369, 486, 548, 594
FauI CCCGC 2 cut(s) 77, 86
FauNDI CATATG 1 cut(s) 530
FbaI TGATCA 1 cut(s) 357
FblI GTMKAC 1 cut(s) 110
FokI GGATG 3 cut(s) 61, 75, 130
FspBI CTAG 1 cut(s) 473
GsuI CTGGAG 1 cut(s) 429
HaeIII GGCC 1 cut(s) 501
HapII CCGG 1 cut(s) 92
HgaI GACGC 1 cut(s) 553
Hin1I GRCGYC 1 cut(s) 545
Hin1II CATG 4 cut(s) 373, 490, 552, 598
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HinfI GANTC 5 cut(s) 138, 159, 167, 373, 617
HpaII CCGG 1 cut(s) 92
HphI GGTGA 2 cut(s) 326, 650
Hpy166II GTNNAC 2 cut(s) 111, 265
Hpy188I TCNGA 2 cut(s) 137, 166
Hpy188III TCNNGA 2 cut(s) 97, 370
Hpy8I GTNNAC 2 cut(s) 111, 265
HpyAV CCTTC 3 cut(s) 355, 584, 649
HpyCH4III ACNGT 3 cut(s) 286, 538, 644
HpyCH4IV ACGT 1 cut(s) 567
HpyCH4V TGCA 3 cut(s) 309, 497, 534
HpyF10VI GCNNNNNNNGC 1 cut(s) 78
HpyF3I CTNAG 3 cut(s) 134, 163, 482
HpySE526I ACGT 1 cut(s) 567
Hsp92I GRCGYC 1 cut(s) 545
Hsp92II CATG 4 cut(s) 373, 490, 552, 598
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 2 cut(s) 357, 612
LpnPI CCDG 7 cut(s) 105, 203, 297, 393, 453, 483, 608
MaeI CTAG 1 cut(s) 473
MaeII ACGT 1 cut(s) 567
MaeIII GTNAC 1 cut(s) 638
MalI GATC 2 cut(s) 359, 614
MbiI CCGCTC 2 cut(s) 72, 81
MboI GATC 2 cut(s) 357, 612
MboII GAAGA 2 cut(s) 146, 517
MluCI AATT 6 cut(s) 9, 58, 200, 272, 292, 600
MlyI GAGTC 3 cut(s) 153, 161, 626
MnlI CCTC 6 cut(s) 158, 197, 374, 404, 555, 629
MseI TTAA 2 cut(s) 438, 453
MspI CCGG 1 cut(s) 92
MwoI GCNNNNNNNGC 1 cut(s) 78
NcoI CCATGG 1 cut(s) 548
NdeI CATATG 1 cut(s) 530
NdeII GATC 2 cut(s) 357, 612
NlaIII CATG 4 cut(s) 373, 490, 552, 598
NlaIV GGNNCC 2 cut(s) 96, 502
NmuCI GTSAC 1 cut(s) 638
PagI TCATGA 1 cut(s) 369
PfeI GAWTC 2 cut(s) 138, 373
PleI GAGTC 3 cut(s) 153, 161, 625
PpsI GAGTC 3 cut(s) 153, 161, 625
PspEI GGTNACC 1 cut(s) 638
PspN4I GGNNCC 2 cut(s) 96, 502
PspPI GGNCC 2 cut(s) 94, 500
PsrI GAACNNNNNNTAC 2 cut(s) 49, 81
RsaI GTAC 3 cut(s) 332, 406, 540
RsaNI GTAC 3 cut(s) 331, 405, 539
SalI GTCGAC 1 cut(s) 109
SaqAI TTAA 2 cut(s) 438, 453
Sau3AI GATC 2 cut(s) 357, 612
Sau96I GGNCC 2 cut(s) 94, 500
SchI GAGTC 3 cut(s) 153, 161, 626
SetI ASST 6 cut(s) 218, 316, 570, 640, 660, 684
SinI GGWCC 1 cut(s) 94
Sse9I AATT 6 cut(s) 9, 58, 200, 272, 292, 600
SsiI CCGC 3 cut(s) 70, 79, 675
SspMI CTAG 1 cut(s) 473
StyI CCWWGG 1 cut(s) 548
TaaI ACNGT 3 cut(s) 286, 538, 644
TaiI ACGT 1 cut(s) 570
TaqI TCGA 4 cut(s) 110, 157, 390, 615
TasI AATT 6 cut(s) 9, 58, 200, 272, 292, 600
TatI WGTACW 1 cut(s) 538
TfiI GAWTC 2 cut(s) 138, 373
Tru1I TTAA 2 cut(s) 438, 453
Tru9I TTAA 2 cut(s) 438, 453
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 1 cut(s) 638
Tsp45I GTSAC 1 cut(s) 638
TspDTI ATGAA 3 cut(s) 358, 452, 517
TspGWI ACGGA 1 cut(s) 287
TspRI CASTG 1 cut(s) 289
VpaK11BI GGWCC 1 cut(s) 94
XapI RAATTY 2 cut(s) 272, 292
XmiI GTMKAC 1 cut(s) 110
XspI CTAG 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.