pycom13g10750

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001652
Physical Location & Seq
Reverse (-)
7241405 .. 7243784
2380 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g10750.1

Sequence Viewer

Length: 810 bp
ATGGCGGCTCCGTTGACCACCCTGTCAAATGTTGGTTCGCTTCCGGCTTCTCGCAGCGTGTCTCAGATCAGAGTTCTGACCCCTACCTGTTCTTCCATCACATTCCCATCGTCATCTAACAAAACGGCGTCGTTGTCCTGCGGCTTCTTCTCCAGGTCGTCGCGTTTGTCCCCTCTGACATTCTCTTCGAACCCTAGTTCCGGGAAAAGGACCCGTCTTGCTAGCTCAGTCCGCGCCAGTGCTGAGGAAGCAACTTTACAATCAAAAGTGACCCATAAGGTGTATTTTGATATTAGCATTGGGAACCCAGTTGGGAAGCTTGCTGGAAGGATTGTGATTGGATTGTATGGCGATGATGTGCCCCAAACCGTGGAGAATTTCCGTGCTCTTTGCACAGGTGATAAAGGCTTTGGATTCAAGGGTTCCGCGTTCCATCGTGTGATCAAGGATTTCATGATTCAAGGAGGGGACTTTGACAAAGGCAATGGCACCGGAGGTAAAAGCATTTACGGACGTACTTTCAAGGATGAGAACTTCAAGTTGTCTCATACTGGCCCTGGAGTTGTTAGCATGGCCAATGCAGGGCCCAATACCAATGGTAGCCAGTTCTTCATATGCACGGTCAAGACACCATGGCTCGACAACAGGCATGTCGTTTTTGGGCAAGTGGTGGAAGGCCTGGATGTTGTTAAACTAATCGAGTCTCAAGAGACAGACAGAGGTCGATCGCCCTACAAAGAAGGTGGTCATCGCCGACTGTGGAGAGCTGCCAGTGGCCTAATGCGGATTTTTATCGGTTTTAGTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pro_isomerase

Protein Analysis

270

Amino Acids

28.98

Weight (kDa)

9.94

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_isomerase PF00160 102 - 246 2.9e-47 Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 22
AccB1I GGYRCC 1 cut(s) 488
AccB7I CCANNNNNTGG 1 cut(s) 370
AccII CGCG 3 cut(s) 163, 234, 428
AciI CCGC 5 cut(s) 5, 141, 232, 426, 784
AcoI YGGCCR 1 cut(s) 573
AcsI RAATTY 1 cut(s) 376
AcyI GRCGYC 1 cut(s) 128
AfaI GTAC 1 cut(s) 517
AfiI CCNNNNNNNGG 4 cut(s) 200, 207, 370, 582
AgsI TTSAA 4 cut(s) 418, 461, 523, 538
AjnI CCWGG 3 cut(s) 152, 556, 678
AloI GAACNNNNNNTCC 2 cut(s) 182, 214
AluBI AGCT 3 cut(s) 225, 319, 767
AluI AGCT 3 cut(s) 225, 319, 767
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 4 cut(s) 66, 549, 704, 708
AoxI GGCC 5 cut(s) 553, 573, 584, 676, 775
ApaI GGGCCC 1 cut(s) 588
ApeKI GCWGC 2 cut(s) 54, 767
ApoI RAATTY 1 cut(s) 376
ArsI GACNNNNNNTTYG 2 cut(s) 169, 201
AspLEI GCGC 1 cut(s) 236
AspS9I GGNCC 4 cut(s) 210, 554, 584, 585
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 1 cut(s) 410
AsuII TTCGAA 1 cut(s) 188
AsuNHI GCTAGC 1 cut(s) 221
AvaII GGWCC 1 cut(s) 210
BaeGI GKGCMC 2 cut(s) 363, 588
BalI TGGCCA 1 cut(s) 575
BanI GGYRCC 1 cut(s) 488
BanII GRGCYC 1 cut(s) 588
BarI GAAGNNNNNNTAC 2 cut(s) 240, 272
Bbv12I GWGCWC 1 cut(s) 388
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 2 cut(s) 66, 754
BccI CCATC 3 cut(s) 104, 115, 441
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 3 cut(s) 154, 558, 680
BclI TGATCA 1 cut(s) 441
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 4 cut(s) 66, 549, 704, 708
BfaI CTAG 3 cut(s) 195, 222, 808
BisI GCNGC 4 cut(s) 6, 55, 142, 768
BlsI GCNGC 4 cut(s) 7, 56, 143, 769
Bme1390I CCNGG 4 cut(s) 154, 202, 558, 680
Bme18I GGWCC 1 cut(s) 210
BmgT120I GGNCC 4 cut(s) 210, 554, 584, 585
BmiI GGNNCC 6 cut(s) 9, 212, 305, 424, 490, 586
BmrFI CCNGG 4 cut(s) 154, 202, 558, 680
BmrI ACTGGG 1 cut(s) 302
BmtI GCTAGC 1 cut(s) 225
BmuI ACTGGG 1 cut(s) 302
BoxI GACNNNNGTC 1 cut(s) 720
BpmI CTGGAG 2 cut(s) 136, 579
Bpu10I CCTNAGC 1 cut(s) 243
Bpu14I TTCGAA 1 cut(s) 188
BpuEI CTTGAG 1 cut(s) 690
BpuMI CCSGG 1 cut(s) 202
BsaBI GATNNNNATC 1 cut(s) 791
BsaHI GRCGYC 1 cut(s) 128
BsaJI CCNNGG 3 cut(s) 369, 556, 632
BsaWI WCCGGW 1 cut(s) 491
Bsc4I CCNNNNNNNGG 4 cut(s) 200, 207, 370, 582
Bse1I ACTGG 5 cut(s) 237, 308, 556, 604, 771
Bse3DI GCAATG 1 cut(s) 490
Bse8I GATNNNNATC 1 cut(s) 791
BseBI CCWGG 3 cut(s) 154, 558, 680
BseDI CCNNGG 3 cut(s) 369, 556, 632
BseGI GGATG 2 cut(s) 532, 688
BseJI GATNNNNATC 1 cut(s) 791
BseLI CCNNNNNNNGG 4 cut(s) 200, 207, 370, 582
BseMI GCAATG 1 cut(s) 490
BseMII CTCAG 3 cut(s) 77, 234, 240
BseNI ACTGG 5 cut(s) 237, 308, 556, 604, 771
BseSI GKGCMC 2 cut(s) 363, 588
BseXI GCAGC 2 cut(s) 66, 754
Bsh1236I CGCG 3 cut(s) 163, 234, 428
Bsh1285I CGRYCG 1 cut(s) 728
BshFI GGCC 5 cut(s) 555, 575, 586, 678, 777
BshNI GGYRCC 1 cut(s) 488
BsiEI CGRYCG 1 cut(s) 728
BsiHKAI GWGCWC 1 cut(s) 388
BsiSI CCGG 3 cut(s) 44, 201, 492
BslFI GGGAC 2 cut(s) 154, 482
BslI CCNNNNNNNGG 4 cut(s) 200, 207, 370, 582
BsmAI GTCTC 4 cut(s) 66, 549, 704, 708
BsmFI GGGAC 2 cut(s) 154, 482
BsnI GGCC 5 cut(s) 555, 575, 586, 678, 777
Bsp119I TTCGAA 1 cut(s) 188
Bsp120I GGGCCC 1 cut(s) 584
Bsp1286I GDGCHC 3 cut(s) 363, 388, 588
Bsp143I GATC 3 cut(s) 66, 441, 725
Bsp19I CCATGG 1 cut(s) 632
BspACI CCGC 5 cut(s) 5, 141, 232, 426, 784
BspANI GGCC 5 cut(s) 555, 575, 586, 678, 777
BspCNI CTCAG 3 cut(s) 76, 235, 239
BspFNI CGCG 3 cut(s) 163, 234, 428
BspHI TCATGA 1 cut(s) 453
BspLI GGNNCC 6 cut(s) 9, 212, 305, 424, 490, 586
BspOI GCTAGC 1 cut(s) 225
BspT104I TTCGAA 1 cut(s) 188
BspT107I GGYRCC 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 490
BsrI ACTGG 5 cut(s) 237, 308, 556, 604, 771
BssECI CCNNGG 3 cut(s) 369, 556, 632
BssMI GATC 3 cut(s) 66, 441, 725
BssNI GRCGYC 1 cut(s) 128
BssT1I CCWWGG 1 cut(s) 632
Bst2UI CCWGG 3 cut(s) 154, 558, 680
Bst4CI ACNGT 3 cut(s) 370, 622, 759
Bst6I CTCTTC 1 cut(s) 190
BstACI GRCGYC 1 cut(s) 128
BstBI TTCGAA 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 223, 321
BstDEI CTNAG 3 cut(s) 63, 226, 243
BstDSI CCRYGG 2 cut(s) 369, 632
BstF5I GGATG 2 cut(s) 532, 688
BstFNI CGCG 3 cut(s) 163, 234, 428
BstHHI GCGC 1 cut(s) 236
BstKTI GATC 3 cut(s) 69, 444, 728
BstMAI GTCTC 4 cut(s) 66, 549, 704, 708
BstMBI GATC 3 cut(s) 66, 441, 725
BstMCI CGRYCG 1 cut(s) 728
BstMWI GCNNNNNNNGC 2 cut(s) 231, 248
BstNI CCWGG 3 cut(s) 154, 558, 680
BstNSI RCATGY 1 cut(s) 653
BstPAI GACNNNNGTC 1 cut(s) 720
BstSCI CCNGG 4 cut(s) 152, 200, 556, 678
BstSLI GKGCMC 2 cut(s) 363, 588
BstUI CGCG 3 cut(s) 163, 234, 428
BstV1I GCAGC 2 cut(s) 66, 754
BsuRI GGCC 5 cut(s) 555, 575, 586, 678, 777
BtgI CCRYGG 2 cut(s) 369, 632
BtgZI GCGATG 2 cut(s) 366, 734
BtsCI GGATG 2 cut(s) 532, 688
BtsIMutI CAGTG 2 cut(s) 244, 778
Cac8I GCNNGC 2 cut(s) 223, 321
CciI TCATGA 1 cut(s) 453
CfoI GCGC 1 cut(s) 236
Cfr13I GGNCC 4 cut(s) 210, 554, 584, 585
CseI GACGC 1 cut(s) 117
Csp6I GTAC 1 cut(s) 516
CspCI CAANNNNNGTGG 2 cut(s) 724, 759
CviAII CATG 4 cut(s) 454, 571, 633, 650
CviQI GTAC 1 cut(s) 516
DdeI CTNAG 3 cut(s) 63, 226, 243
DpnI GATC 3 cut(s) 68, 443, 727
DpnII GATC 3 cut(s) 66, 441, 725
DrdI GACNNNNNNGTC 1 cut(s) 22
DseDI GACNNNNNNGTC 1 cut(s) 22
EaeI YGGCCR 1 cut(s) 573
Eam1104I CTCTTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 632
Eco147I AGGCCT 1 cut(s) 678
Eco24I GRGCYC 1 cut(s) 588
Eco47I GGWCC 1 cut(s) 210
EcoO109I RGGNCCY 2 cut(s) 210, 584
EcoRII CCWGG 3 cut(s) 152, 556, 678
EcoT14I CCWWGG 1 cut(s) 632
EcoT38I GRGCYC 1 cut(s) 588
ErhI CCWWGG 1 cut(s) 632
FaeI CATG 4 cut(s) 457, 574, 636, 653
FaiI YATR 9 cut(s) 276, 348, 455, 549, 572, 614, 616, 634, 651
FaqI GGGAC 2 cut(s) 154, 482
FatI CATG 4 cut(s) 453, 570, 632, 649
FauNDI CATATG 1 cut(s) 614
FbaI TGATCA 1 cut(s) 441
Fnu4HI GCNGC 4 cut(s) 6, 55, 142, 768
FokI GGATG 2 cut(s) 539, 695
FriOI GRGCYC 1 cut(s) 588
Fsp4HI GCNGC 4 cut(s) 6, 55, 142, 768
FspBI CTAG 3 cut(s) 195, 222, 808
GlaI GCGC 1 cut(s) 235
GluI GCNGC 4 cut(s) 6, 55, 142, 768
GsuI CTGGAG 2 cut(s) 136, 579
HaeIII GGCC 5 cut(s) 555, 575, 586, 678, 777
HapII CCGG 3 cut(s) 44, 201, 492
HgaI GACGC 1 cut(s) 117
HhaI GCGC 1 cut(s) 236
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 4 cut(s) 457, 574, 636, 653
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HindIII AAGCTT 1 cut(s) 317
HinfI GANTC 3 cut(s) 414, 457, 701
HpaII CCGG 3 cut(s) 44, 201, 492
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 1 cut(s) 15
Hpy188I TCNGA 4 cut(s) 66, 71, 78, 177
Hpy188III TCNNGA 3 cut(s) 454, 625, 707
Hpy8I GTNNAC 1 cut(s) 15
Hpy99I CGWCG 2 cut(s) 133, 163
HpyAV CCTTC 3 cut(s) 321, 668, 734
HpyCH4III ACNGT 3 cut(s) 370, 622, 759
HpyCH4IV ACGT 1 cut(s) 514
HpyCH4V TGCA 3 cut(s) 393, 581, 618
HpyF10VI GCNNNNNNNGC 2 cut(s) 231, 248
HpyF3I CTNAG 3 cut(s) 63, 226, 243
HpySE526I ACGT 1 cut(s) 514
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 4 cut(s) 457, 574, 636, 653
HspAI GCGC 1 cut(s) 234
Ksp22I TGATCA 1 cut(s) 441
Kzo9I GATC 3 cut(s) 66, 441, 725
LmnI GCTCC 1 cut(s) 13
Lsp1109I GCAGC 2 cut(s) 66, 754
MaeI CTAG 3 cut(s) 195, 222, 808
MaeII ACGT 1 cut(s) 514
MaeIII GTNAC 2 cut(s) 268, 803
MalI GATC 3 cut(s) 68, 443, 727
MboI GATC 3 cut(s) 66, 441, 725
MboII GAAGA 4 cut(s) 84, 139, 177, 601
MhlI GDGCHC 3 cut(s) 363, 388, 588
MlsI TGGCCA 1 cut(s) 575
MluCI AATT 1 cut(s) 376
MluNI TGGCCA 1 cut(s) 575
MlyI GAGTC 1 cut(s) 710
MnlI CCTC 5 cut(s) 183, 238, 458, 488, 713
Mox20I TGGCCA 1 cut(s) 575
MscI TGGCCA 1 cut(s) 575
MseI TTAA 1 cut(s) 690
Msp20I TGGCCA 1 cut(s) 575
MspI CCGG 3 cut(s) 44, 201, 492
MspR9I CCNGG 4 cut(s) 154, 202, 558, 680
MvaI CCWGG 3 cut(s) 154, 558, 680
MvnI CGCG 3 cut(s) 163, 234, 428
MwoI GCNNNNNNNGC 2 cut(s) 231, 248
NciI CCSGG 1 cut(s) 202
NcoI CCATGG 1 cut(s) 632
NdeI CATATG 1 cut(s) 614
NdeII GATC 3 cut(s) 66, 441, 725
NheI GCTAGC 1 cut(s) 221
NlaIII CATG 4 cut(s) 457, 574, 636, 653
NlaIV GGNNCC 6 cut(s) 9, 212, 305, 424, 490, 586
NmuCI GTSAC 1 cut(s) 268
NspI RCATGY 1 cut(s) 653
NspV TTCGAA 1 cut(s) 188
PagI TCATGA 1 cut(s) 453
PceI AGGCCT 1 cut(s) 678
PfeI GAWTC 2 cut(s) 414, 457
PflMI CCANNNNNTGG 1 cut(s) 370
PfoI TCCNGGA 1 cut(s) 200
PkrI GCNGC 4 cut(s) 7, 56, 143, 769
Ple19I CGATCG 1 cut(s) 728
PleI GAGTC 1 cut(s) 709
PpsI GAGTC 1 cut(s) 709
PpuMI RGGWCCY 1 cut(s) 210
PshAI GACNNNNGTC 1 cut(s) 720
Psp5II RGGWCCY 1 cut(s) 210
Psp6I CCWGG 3 cut(s) 152, 556, 678
PspGI CCWGG 3 cut(s) 152, 556, 678
PspN4I GGNNCC 6 cut(s) 9, 212, 305, 424, 490, 586
PspOMI GGGCCC 1 cut(s) 584
PspPI GGNCC 4 cut(s) 210, 554, 584, 585
PspPPI RGGWCCY 1 cut(s) 210
PvuI CGATCG 1 cut(s) 728
RsaI GTAC 1 cut(s) 517
RsaNI GTAC 1 cut(s) 516
SaqAI TTAA 1 cut(s) 690
SatI GCNGC 4 cut(s) 6, 55, 142, 768
Sau3AI GATC 3 cut(s) 66, 441, 725
Sau96I GGNCC 4 cut(s) 210, 554, 584, 585
SchI GAGTC 1 cut(s) 710
ScrFI CCNGG 4 cut(s) 154, 202, 558, 680
SduI GDGCHC 3 cut(s) 363, 388, 588
SfuI TTCGAA 1 cut(s) 188
SinI GGWCC 1 cut(s) 210
SmlI CTYRAG 1 cut(s) 705
SmoI CTYRAG 1 cut(s) 705
Sse9I AATT 1 cut(s) 376
SseBI AGGCCT 1 cut(s) 678
SsiI CCGC 5 cut(s) 5, 141, 232, 426, 784
SspMI CTAG 3 cut(s) 195, 222, 808
StuI AGGCCT 1 cut(s) 678
StyD4I CCNGG 4 cut(s) 152, 200, 556, 678
StyI CCWWGG 1 cut(s) 632
TaaI ACNGT 3 cut(s) 370, 622, 759
TaiI ACGT 1 cut(s) 517
TaqI TCGA 4 cut(s) 188, 639, 699, 724
TasI AATT 1 cut(s) 376
TauI GCSGC 2 cut(s) 8, 144
TfiI GAWTC 2 cut(s) 414, 457
Tru1I TTAA 1 cut(s) 690
Tru9I TTAA 1 cut(s) 690
TscAI CASTG 2 cut(s) 244, 778
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 2 cut(s) 54, 767
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 2 cut(s) 442, 601
TspGWI ACGGA 2 cut(s) 371, 525
TspRI CASTG 2 cut(s) 244, 778
Van91I CCANNNNNTGG 1 cut(s) 370
VpaK11BI GGWCC 1 cut(s) 210
XapI RAATTY 1 cut(s) 376
XceI RCATGY 1 cut(s) 653
XspI CTAG 3 cut(s) 195, 222, 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.