Rw2G028480

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001652
Physical Location & Seq
Forward (+)
45599078 .. 45603035
3958 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G028480.1

Sequence Viewer

Length: 537 bp
ATGGCTACGGCATATTCTTTCCTCGACCATAGCAATCTCTCAAACTCTCAAACTCTCAAACTCTTAGAATCTCATCCCTCTCTCTCCGACTCTCAGACTCACTCTCCCCCAATCTCCTCCGCGGATGTTGAGAATTTCCGTTCCCTTTGCACAGGTGAGAAAGGCTTTGGGTACAAGGGTTCTGCCTTCCATCGTGTGATCAAGGATTTCATGATTCAAGGAGGGAACTTCGATAAGGGCAATGGTACTGGAGGCAAAAGCATTTATGGGCGTACATTTAAAGATGAGAACTTTAAGTTGCTTCATACTGGACCAGGAGTTCTTAGCATGGCAAATGCAGGCCCCAATACCAATGGAAGTCAGTTCTTCATATGCACAGTACAGACGCCATGGCTAAATGGGAGGCACGTTGTGTTTGGGCAAGTTTTGGAAGGCATGGAAATTGTTAGGCTGATCGAGTCACAGGAGACAGATAGAGGTGACCGTCCTACAAAGAAGGTAGTCATTGCTGACTGCGGAGAGCTTCCAGTAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pro_isomerase

Protein Analysis

178

Amino Acids

19.34

Weight (kDa)

6.58

Isoelectric Point (pI)

20.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_isomerase PF00160 42 - 173 3.7e-44 Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 122
AciI CCGC 3 cut(s) 120, 122, 516
AcsI RAATTY 1 cut(s) 133
AcyI GRCGYC 1 cut(s) 386
AdeI CACNNNGTG 1 cut(s) 412
AfaI GTAC 4 cut(s) 173, 247, 274, 381
AgsI TTSAA 1 cut(s) 218
AjnI CCWGG 1 cut(s) 313
AloI GAACNNNNNNTCC 2 cut(s) 303, 335
AluBI AGCT 1 cut(s) 523
AluI AGCT 1 cut(s) 523
Alw26I GTCTC 1 cut(s) 461
AoxI GGCC 1 cut(s) 340
ApoI RAATTY 1 cut(s) 133
AspS9I GGNCC 2 cut(s) 311, 341
AsuHPI GGTGA 2 cut(s) 167, 491
AvaII GGWCC 1 cut(s) 311
BaeI ACNNNNGTAYC 2 cut(s) 163, 196
BccI CCATC 1 cut(s) 198
BceAI ACGGC 1 cut(s) 24
BciT130I CCWGG 1 cut(s) 315
BclI TGATCA 1 cut(s) 198
BcoDI GTCTC 1 cut(s) 461
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 311
BmgT120I GGNCC 2 cut(s) 311, 341
BmiI GGNNCC 1 cut(s) 343
BmrFI CCNGG 1 cut(s) 315
BpmI CTGGAG 1 cut(s) 270
BsaHI GRCGYC 1 cut(s) 386
BsaJI CCNNGG 2 cut(s) 120, 389
BsaXI ACNNNNNCTCC 2 cut(s) 88, 118
Bse1I ACTGG 3 cut(s) 253, 313, 527
Bse3DI GCAATG 2 cut(s) 247, 504
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 2 cut(s) 120, 389
BseGI GGATG 2 cut(s) 73, 130
BseMI GCAATG 2 cut(s) 247, 504
BseMII CTCAG 1 cut(s) 107
BseNI ACTGG 3 cut(s) 253, 313, 527
BseRI GAGGAG 1 cut(s) 106
Bsh1236I CGCG 1 cut(s) 122
BshFI GGCC 1 cut(s) 342
BsmAI GTCTC 1 cut(s) 461
BsnI GGCC 1 cut(s) 342
Bsp143I GATC 2 cut(s) 198, 453
Bsp19I CCATGG 1 cut(s) 389
BspACI CCGC 3 cut(s) 120, 122, 516
BspANI GGCC 1 cut(s) 342
BspCNI CTCAG 1 cut(s) 106
BspFNI CGCG 1 cut(s) 122
BspHI TCATGA 1 cut(s) 210
BspLI GGNNCC 1 cut(s) 343
BsrDI GCAATG 2 cut(s) 247, 504
BsrI ACTGG 3 cut(s) 253, 313, 527
BssECI CCNNGG 2 cut(s) 120, 389
BssMI GATC 2 cut(s) 198, 453
BssNI GRCGYC 1 cut(s) 386
BssT1I CCWWGG 1 cut(s) 389
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 2 cut(s) 379, 485
BstACI GRCGYC 1 cut(s) 386
BstC8I GCNNGC 1 cut(s) 340
BstDEI CTNAG 3 cut(s) 64, 93, 323
BstDSI CCRYGG 2 cut(s) 120, 389
BstEII GGTNACC 1 cut(s) 479
BstF5I GGATG 2 cut(s) 73, 130
BstFNI CGCG 1 cut(s) 122
BstKTI GATC 2 cut(s) 201, 456
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 2 cut(s) 198, 453
BstNI CCWGG 1 cut(s) 315
BstPI GGTNACC 1 cut(s) 479
BstSCI CCNGG 1 cut(s) 313
BstUI CGCG 1 cut(s) 122
BsuRI GGCC 1 cut(s) 342
BtgI CCRYGG 2 cut(s) 120, 389
BtsCI GGATG 2 cut(s) 73, 130
Cac8I GCNNGC 1 cut(s) 340
CciI TCATGA 1 cut(s) 210
Cfr13I GGNCC 2 cut(s) 311, 341
Cfr42I CCGCGG 1 cut(s) 123
CseI GACGC 1 cut(s) 394
Csp6I GTAC 4 cut(s) 172, 246, 273, 380
CviAII CATG 4 cut(s) 211, 328, 390, 436
CviJI RGCY 7 cut(s) 5, 165, 342, 394, 451, 523, 533
CviKI_1 RGCY 7 cut(s) 5, 165, 342, 394, 451, 523, 533
CviQI GTAC 4 cut(s) 172, 246, 273, 380
DdeI CTNAG 3 cut(s) 64, 93, 323
DpnI GATC 2 cut(s) 200, 455
DpnII GATC 2 cut(s) 198, 453
DraI TTTAAA 1 cut(s) 280
DraIII CACNNNGTG 1 cut(s) 412
Eco130I CCWWGG 1 cut(s) 389
Eco47I GGWCC 1 cut(s) 311
Eco91I GGTNACC 1 cut(s) 479
EcoO109I RGGNCCY 1 cut(s) 341
EcoO65I GGTNACC 1 cut(s) 479
EcoRII CCWGG 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 389
ErhI CCWWGG 1 cut(s) 389
FaeI CATG 4 cut(s) 214, 331, 393, 439
FatI CATG 4 cut(s) 210, 327, 389, 435
FauNDI CATATG 1 cut(s) 371
FbaI TGATCA 1 cut(s) 198
FokI GGATG 2 cut(s) 60, 137
GsuI CTGGAG 1 cut(s) 270
HaeIII GGCC 1 cut(s) 342
HgaI GACGC 1 cut(s) 394
Hin1I GRCGYC 1 cut(s) 386
Hin1II CATG 4 cut(s) 214, 331, 393, 439
HinfI GANTC 5 cut(s) 68, 89, 97, 214, 458
HphI GGTGA 2 cut(s) 167, 491
Hpy188I TCNGA 2 cut(s) 88, 96
Hpy188III TCNNGA 1 cut(s) 211
HpyAV CCTTC 3 cut(s) 196, 425, 490
HpyCH4III ACNGT 2 cut(s) 379, 485
HpyCH4IV ACGT 1 cut(s) 408
HpyCH4V TGCA 3 cut(s) 150, 338, 375
HpyF3I CTNAG 3 cut(s) 64, 93, 323
HpySE526I ACGT 1 cut(s) 408
Hsp92I GRCGYC 1 cut(s) 386
Hsp92II CATG 4 cut(s) 214, 331, 393, 439
Ksp22I TGATCA 1 cut(s) 198
KspI CCGCGG 1 cut(s) 123
Kzo9I GATC 2 cut(s) 198, 453
LpnPI CCDG 7 cut(s) 138, 234, 294, 300, 324, 327, 449
MaeII ACGT 1 cut(s) 408
MaeIII GTNAC 2 cut(s) 459, 479
MalI GATC 2 cut(s) 200, 455
MboI GATC 2 cut(s) 198, 453
MboII GAAGA 1 cut(s) 358
MluCI AATT 2 cut(s) 133, 441
MlyI GAGTC 3 cut(s) 83, 91, 467
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 7 cut(s) 32, 88, 127, 215, 245, 396, 470
MseI TTAA 2 cut(s) 279, 294
MspA1I CMGCKG 1 cut(s) 122
MspR9I CCNGG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 315
MvnI CGCG 1 cut(s) 122
NcoI CCATGG 1 cut(s) 389
NdeI CATATG 1 cut(s) 371
NdeII GATC 2 cut(s) 198, 453
NlaIII CATG 4 cut(s) 214, 331, 393, 439
NlaIV GGNNCC 1 cut(s) 343
NmuCI GTSAC 2 cut(s) 459, 479
PagI TCATGA 1 cut(s) 210
PfeI GAWTC 2 cut(s) 68, 214
PleI GAGTC 3 cut(s) 83, 91, 466
PpsI GAGTC 3 cut(s) 83, 91, 466
Psp6I CCWGG 1 cut(s) 313
PspEI GGTNACC 1 cut(s) 479
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 343
PspPI GGNCC 2 cut(s) 311, 341
RsaI GTAC 4 cut(s) 173, 247, 274, 381
RsaNI GTAC 4 cut(s) 172, 246, 273, 380
SacII CCGCGG 1 cut(s) 123
SaqAI TTAA 2 cut(s) 279, 294
Sau3AI GATC 2 cut(s) 198, 453
Sau96I GGNCC 2 cut(s) 311, 341
SchI GAGTC 3 cut(s) 83, 91, 467
ScrFI CCNGG 1 cut(s) 315
SetI ASST 5 cut(s) 157, 411, 481, 501, 525
Sfr303I CCGCGG 1 cut(s) 123
SgrBI CCGCGG 1 cut(s) 123
SinI GGWCC 1 cut(s) 311
Sse9I AATT 2 cut(s) 133, 441
SsiI CCGC 3 cut(s) 120, 122, 516
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 1 cut(s) 389
TaaI ACNGT 2 cut(s) 379, 485
TaiI ACGT 1 cut(s) 411
TaqI TCGA 3 cut(s) 24, 231, 456
TasI AATT 2 cut(s) 133, 441
TatI WGTACW 1 cut(s) 379
TfiI GAWTC 2 cut(s) 68, 214
Tru1I TTAA 2 cut(s) 279, 294
Tru9I TTAA 2 cut(s) 279, 294
TseFI GTSAC 2 cut(s) 459, 479
Tsp45I GTSAC 2 cut(s) 459, 479
TspDTI ATGAA 3 cut(s) 199, 293, 358
TspGWI ACGGA 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 311
XapI RAATTY 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.