MD16G1055800.v1.1

Belongs to the sulfotransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001120
Physical Location & Seq
Forward (+)
3979752 .. 3980747
996 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1055800.v1.1.491

Sequence Viewer

Length: 996 bp
ATGAAAACACCTCAACCTCCTCCTGTTGTTCCCCGATACTTGCAAGAAAATGAACCTAGCCAAGAAGACAAAGACCTTATATCCTCTCTACCTACAGAAAAAGGTTGGATTGGAAATAGCCTTCACAAGTACCAAGGTTTTTGGCAGGCAACTGCGCACATGCAGGGAGTTTTGGCATGCCAGAAACACTTTCGAGCTCTAGAATCTGATATCATTCTCGTTACCACTCCCAAATCAGGCACGACTTGGCTCATGGCAATTTTGTTTGCCCTAGTGAAACGAGCGCACTATCTGGACCTTCGGCGACACCCTTTGCTCACAGAAAACCCTCATGTTCTTGTGCCCTGTTTGGAGTTAGATGTCTACACTGACAAACAGGTACTTCCTGACCTCTCCTCCCTGGCCCCTCCGAGGCTCTTTTCGACTCATTTACCGTATGTGTCTCTGCCGGATTCTGTCAAACATTCCGGTTGCAAAGTTGTTTACTTGTCTAGAGATCCTAAAGATGCTTTTGTTTCACTTTGGCACTTCGTGAACAAACTGAGACCCGTGGGTAGTGGAACCATGTCACTTCACGAAGCGTTCGATAAGTTCTGCCGGGGAGTGAGTTCGTTCGGACCCTTTTGGGATCATGTACTGGGGTTTTGGAACGAGAGCTTAAAGAGACCTGAAAGTGCATTTTTCATCAAGTTTGAGGAAATGAAACAAGAGCCCGCTCTTCAACTGAGGAGGCTGGCCGAGTTCTTGGGGTGCCCATTTTCCCCGGAAGAAGAGACGCATGGAATTGTGGACGGTATCTTAAGGTTGTGTAGTTTTGACAATTTAAGCAATTTGGTTGTGAATAAAAGTGGGAAATTGCCGGCCGGTATGGAGAATAGTGCATTCTTTAGGAAAGGTGAAGTTGGAGATTCGACGAATTATTTGACCACTGAGATGGTGAAAAAACTAGACTCCATTACTCAAGAGAAGTTGTATGGTTCCGGGCTAAAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sulfotransfer_1

Protein Analysis

332

Amino Acids

37.45

Weight (kDa)

7.19

Isoelectric Point (pI)

51.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sulfotransfer_1 PF00685 69 - 328 5.8e-69 Sulfotransferase domain
Sulfotransfer_3 PF13469 159 - 254 9.3e-06 Sulfotransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 156
AccB1I GGYRCC 1 cut(s) 750
AccBSI CCGCTC 1 cut(s) 716
AccI GTMKAC 1 cut(s) 363
AciI CCGC 1 cut(s) 714
AclWI GGATC 2 cut(s) 491, 636
AcoI YGGCCR 2 cut(s) 735, 861
AdeI CACNNNGTG 1 cut(s) 532
AfaI GTAC 3 cut(s) 131, 381, 636
AfiI CCNNNNNNNGG 2 cut(s) 236, 411
AflII CTTAAG 1 cut(s) 799
AgsI TTSAA 1 cut(s) 722
AjnI CCWGG 1 cut(s) 399
AluBI AGCT 2 cut(s) 197, 657
AluI AGCT 2 cut(s) 197, 657
Alw21I GWGCWC 1 cut(s) 199
Alw26I GTCTC 4 cut(s) 447, 538, 658, 767
AlwI GGATC 2 cut(s) 491, 636
AoxI GGCC 3 cut(s) 402, 735, 861
ArsI GACNNNNNNTTYG 2 cut(s) 904, 936
AspLEI GCGC 2 cut(s) 157, 286
AspS9I GGNCC 3 cut(s) 295, 403, 617
AsuC2I CCSGG 3 cut(s) 599, 764, 982
AsuHPI GGTGA 2 cut(s) 908, 949
AvaII GGWCC 2 cut(s) 295, 617
BaeGI GKGCMC 2 cut(s) 345, 755
BanI GGYRCC 1 cut(s) 750
BanII GRGCYC 2 cut(s) 199, 714
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 199
BccI CCATC 1 cut(s) 928
BciT130I CCWGG 1 cut(s) 401
BcnI CCSGG 3 cut(s) 599, 764, 982
BcoDI GTCTC 4 cut(s) 447, 538, 658, 767
BfaI CTAG 6 cut(s) 57, 200, 272, 492, 947, 994
BfmI CTRYAG 1 cut(s) 93
BfrI CTTAAG 1 cut(s) 799
Bme1390I CCNGG 4 cut(s) 401, 599, 764, 982
Bme18I GGWCC 2 cut(s) 295, 617
BmgT120I GGNCC 3 cut(s) 295, 403, 617
BmiI GGNNCC 5 cut(s) 405, 562, 619, 752, 979
BmrFI CCNGG 4 cut(s) 401, 599, 764, 982
BmrI ACTGGG 1 cut(s) 647
BmsI GCATC 1 cut(s) 496
BmuI ACTGGG 1 cut(s) 647
BpiI GAAGAC 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 945
BpuMI CCSGG 3 cut(s) 599, 764, 982
BsaI GGTCTC 2 cut(s) 538, 658
BsaJI CCNNGG 6 cut(s) 133, 399, 410, 549, 598, 762
BsaWI WCCGGW 1 cut(s) 467
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 2 cut(s) 236, 411
Bse118I RCCGGY 2 cut(s) 859, 863
Bse1I ACTGG 1 cut(s) 642
BseBI CCWGG 1 cut(s) 401
BseDI CCNNGG 6 cut(s) 133, 399, 410, 549, 598, 762
BseLI CCNNNNNNNGG 2 cut(s) 236, 411
BseMII CTCAG 3 cut(s) 533, 716, 921
BseNI ACTGG 1 cut(s) 642
BseRI GAGGAG 3 cut(s) 9, 385, 742
BseSI GKGCMC 2 cut(s) 345, 755
BseX3I CGGCCG 1 cut(s) 861
Bsh1285I CGRYCG 1 cut(s) 864
BshFI GGCC 3 cut(s) 404, 737, 863
BshNI GGYRCC 1 cut(s) 750
BsiEI CGRYCG 1 cut(s) 864
BsiHKAI GWGCWC 1 cut(s) 199
BsiSI CCGG 7 cut(s) 449, 468, 598, 764, 860, 864, 981
BslI CCNNNNNNNGG 2 cut(s) 236, 411
BsmAI GTCTC 4 cut(s) 447, 538, 658, 767
BsmBI CGTCTC 1 cut(s) 767
BsmI GAATGC 1 cut(s) 881
BsnI GGCC 3 cut(s) 404, 737, 863
Bso31I GGTCTC 2 cut(s) 538, 658
Bsp1286I GDGCHC 4 cut(s) 199, 345, 714, 755
Bsp143I GATC 2 cut(s) 496, 628
BspACI CCGC 1 cut(s) 714
BspANI GGCC 3 cut(s) 404, 737, 863
BspCNI CTCAG 3 cut(s) 534, 717, 922
BspLI GGNNCC 5 cut(s) 405, 562, 619, 752, 979
BspPI GGATC 2 cut(s) 491, 636
BspQI GCTCTTC 1 cut(s) 723
BspT107I GGYRCC 1 cut(s) 750
BspTI CTTAAG 1 cut(s) 799
BspTNI GGTCTC 2 cut(s) 538, 658
BsrBI CCGCTC 1 cut(s) 716
BsrFI RCCGGY 2 cut(s) 859, 863
BsrI ACTGG 1 cut(s) 642
BssAI RCCGGY 2 cut(s) 859, 863
BssECI CCNNGG 6 cut(s) 133, 399, 410, 549, 598, 762
BssMI GATC 2 cut(s) 496, 628
BssT1I CCWWGG 1 cut(s) 133
Bst2UI CCWGG 1 cut(s) 401
Bst4CI ACNGT 2 cut(s) 435, 794
Bst6I CTCTTC 2 cut(s) 723, 765
BstAFI CTTAAG 1 cut(s) 799
BstC8I GCNNGC 5 cut(s) 147, 178, 714, 735, 861
BstDEI CTNAG 3 cut(s) 542, 725, 930
BstDSI CCRYGG 1 cut(s) 549
BstHHI GCGC 2 cut(s) 157, 286
BstKTI GATC 2 cut(s) 499, 631
BstMAI GTCTC 4 cut(s) 447, 538, 658, 767
BstMBI GATC 2 cut(s) 496, 628
BstMCI CGRYCG 1 cut(s) 864
BstNI CCWGG 1 cut(s) 401
BstNSI RCATGY 2 cut(s) 163, 180
BstSCI CCNGG 4 cut(s) 399, 597, 762, 980
BstSFI CTRYAG 1 cut(s) 93
BstSLI GKGCMC 2 cut(s) 345, 755
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 1 cut(s) 496
BstXI CCANNNNNNTGG 1 cut(s) 934
BstYI RGATCY 1 cut(s) 496
BstZI CGGCCG 1 cut(s) 861
BsuRI GGCC 3 cut(s) 404, 737, 863
BtgI CCRYGG 1 cut(s) 549
BtsIMutI CAGTG 2 cut(s) 366, 927
Cac8I GCNNGC 5 cut(s) 147, 178, 714, 735, 861
CfoI GCGC 2 cut(s) 157, 286
Cfr10I RCCGGY 2 cut(s) 859, 863
Cfr13I GGNCC 3 cut(s) 295, 403, 617
CseI GACGC 1 cut(s) 784
Csp6I GTAC 3 cut(s) 130, 380, 635
CviAII CATG 7 cut(s) 160, 177, 253, 332, 565, 632, 779
CviQI GTAC 3 cut(s) 130, 380, 635
DdeI CTNAG 3 cut(s) 542, 725, 930
DpnI GATC 2 cut(s) 498, 630
DpnII GATC 2 cut(s) 496, 628
DraIII CACNNNGTG 1 cut(s) 532
EaeI YGGCCR 2 cut(s) 735, 861
EagI CGGCCG 1 cut(s) 861
Eam1104I CTCTTC 2 cut(s) 723, 765
EarI CTCTTC 2 cut(s) 723, 765
Ecl136II GAGCTC 1 cut(s) 197
EclXI CGGCCG 1 cut(s) 861
Eco130I CCWWGG 1 cut(s) 133
Eco24I GRGCYC 2 cut(s) 199, 714
Eco31I GGTCTC 2 cut(s) 538, 658
Eco32I GATATC 1 cut(s) 211
Eco47I GGWCC 2 cut(s) 295, 617
Eco52I CGGCCG 1 cut(s) 861
Eco53kI GAGCTC 1 cut(s) 197
EcoICRI GAGCTC 1 cut(s) 197
EcoRII CCWGG 1 cut(s) 399
EcoRV GATATC 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 133
EcoT38I GRGCYC 2 cut(s) 199, 714
ErhI CCWWGG 1 cut(s) 133
Esp3I CGTCTC 1 cut(s) 767
FaeI CATG 7 cut(s) 163, 180, 256, 335, 568, 635, 782
FatI CATG 7 cut(s) 159, 176, 252, 331, 564, 631, 778
FauI CCCGC 1 cut(s) 721
FblI GTMKAC 1 cut(s) 363
FriOI GRGCYC 2 cut(s) 199, 714
FspBI CTAG 6 cut(s) 57, 200, 272, 492, 947, 994
FspI TGCGCA 1 cut(s) 156
GlaI GCGC 2 cut(s) 156, 285
HaeIII GGCC 3 cut(s) 404, 737, 863
HapII CCGG 7 cut(s) 449, 468, 598, 764, 860, 864, 981
HgaI GACGC 1 cut(s) 784
HhaI GCGC 2 cut(s) 157, 286
Hin1II CATG 7 cut(s) 163, 180, 256, 335, 568, 635, 782
Hin6I GCGC 2 cut(s) 155, 284
HinP1I GCGC 2 cut(s) 155, 284
HinfI GANTC 5 cut(s) 203, 424, 452, 908, 950
HpaII CCGG 7 cut(s) 449, 468, 598, 764, 860, 864, 981
HphI GGTGA 2 cut(s) 908, 949
Hpy166II GTNNAC 4 cut(s) 364, 484, 535, 790
Hpy188I TCNGA 3 cut(s) 208, 411, 617
Hpy188III TCNNGA 7 cut(s) 200, 293, 386, 492, 532, 575, 962
Hpy8I GTNNAC 4 cut(s) 364, 484, 535, 790
Hpy99I CGWCG 1 cut(s) 916
HpyAV CCTTC 2 cut(s) 131, 308
HpyCH4III ACNGT 2 cut(s) 435, 794
HpyCH4V TGCA 5 cut(s) 43, 163, 474, 677, 881
HpyF3I CTNAG 3 cut(s) 542, 725, 930
Hsp92II CATG 7 cut(s) 163, 180, 256, 335, 568, 635, 782
HspAI GCGC 2 cut(s) 155, 284
KroI GCCGGC 1 cut(s) 859
KroNI GCCGGC 1 cut(s) 861
Kzo9I GATC 2 cut(s) 496, 628
LguI GCTCTTC 1 cut(s) 723
LweI GCATC 1 cut(s) 496
MaeI CTAG 6 cut(s) 57, 200, 272, 492, 947, 994
MaeIII GTNAC 2 cut(s) 220, 567
MalI GATC 2 cut(s) 498, 630
MbiI CCGCTC 1 cut(s) 716
MboI GATC 2 cut(s) 496, 628
MboII GAAGA 4 cut(s) 77, 710, 779, 782
MflI RGATCY 1 cut(s) 496
MhlI GDGCHC 4 cut(s) 199, 345, 714, 755
MluCI AATT 6 cut(s) 258, 783, 820, 829, 854, 916
MlyI GAGTC 2 cut(s) 418, 944
MmeI TCCRAC 2 cut(s) 86, 883
MroNI GCCGGC 1 cut(s) 859
MseI TTAA 3 cut(s) 659, 800, 824
MslI CAYNNNNRTG 1 cut(s) 932
MspCI CTTAAG 1 cut(s) 799
MspI CCGG 7 cut(s) 449, 468, 598, 764, 860, 864, 981
MspR9I CCNGG 4 cut(s) 401, 599, 764, 982
Mva1269I GAATGC 1 cut(s) 881
MvaI CCWGG 1 cut(s) 401
NaeI GCCGGC 1 cut(s) 861
NciI CCSGG 3 cut(s) 599, 764, 982
NdeII GATC 2 cut(s) 496, 628
NgoMIV GCCGGC 1 cut(s) 859
NlaIII CATG 7 cut(s) 163, 180, 256, 335, 568, 635, 782
NlaIV GGNNCC 5 cut(s) 405, 562, 619, 752, 979
NmeAIII GCCGAG 1 cut(s) 763
NmuCI GTSAC 1 cut(s) 567
NsbI TGCGCA 1 cut(s) 156
NspI RCATGY 2 cut(s) 163, 180
PaeI GCATGC 1 cut(s) 180
PciSI GCTCTTC 1 cut(s) 723
PcsI WCGNNNNNNNCGW 1 cut(s) 582
PctI GAATGC 1 cut(s) 881
PdiI GCCGGC 1 cut(s) 861
PfeI GAWTC 3 cut(s) 203, 452, 908
PleI GAGTC 2 cut(s) 418, 944
PpsI GAGTC 2 cut(s) 418, 944
Psp124BI GAGCTC 1 cut(s) 199
Psp6I CCWGG 1 cut(s) 399
PspGI CCWGG 1 cut(s) 399
PspN4I GGNNCC 5 cut(s) 405, 562, 619, 752, 979
PspPI GGNCC 3 cut(s) 295, 403, 617
PsuI RGATCY 1 cut(s) 496
RsaI GTAC 3 cut(s) 131, 381, 636
RsaNI GTAC 3 cut(s) 130, 380, 635
RseI CAYNNNNRTG 1 cut(s) 932
SacI GAGCTC 1 cut(s) 199
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 3 cut(s) 659, 800, 824
Sau3AI GATC 2 cut(s) 496, 628
Sau96I GGNCC 3 cut(s) 295, 403, 617
SchI GAGTC 2 cut(s) 418, 944
ScrFI CCNGG 4 cut(s) 401, 599, 764, 982
SduI GDGCHC 4 cut(s) 199, 345, 714, 755
SfaNI GCATC 1 cut(s) 496
SfcI CTRYAG 1 cut(s) 93
SinI GGWCC 2 cut(s) 295, 617
SmiMI CAYNNNNRTG 1 cut(s) 932
SmlI CTYRAG 2 cut(s) 799, 960
SmoI CTYRAG 2 cut(s) 799, 960
SphI GCATGC 1 cut(s) 180
Sse9I AATT 6 cut(s) 258, 783, 820, 829, 854, 916
SsiI CCGC 1 cut(s) 714
SspMI CTAG 6 cut(s) 57, 200, 272, 492, 947, 994
SstI GAGCTC 1 cut(s) 199
StyD4I CCNGG 4 cut(s) 399, 597, 762, 980
StyI CCWWGG 1 cut(s) 133
TaaI ACNGT 2 cut(s) 435, 794
TaqI TCGA 4 cut(s) 193, 422, 585, 911
TasI AATT 6 cut(s) 258, 783, 820, 829, 854, 916
TatI WGTACW 1 cut(s) 634
TfiI GAWTC 3 cut(s) 203, 452, 908
Tru1I TTAA 3 cut(s) 659, 800, 824
Tru9I TTAA 3 cut(s) 659, 800, 824
TscAI CASTG 2 cut(s) 373, 934
TseFI GTSAC 1 cut(s) 567
Tsp45I GTSAC 1 cut(s) 567
TspDTI ATGAA 4 cut(s) 17, 66, 673, 716
TspRI CASTG 2 cut(s) 373, 934
Vha464I CTTAAG 1 cut(s) 799
VpaK11BI GGWCC 2 cut(s) 295, 617
XbaI TCTAGA 2 cut(s) 199, 491
XceI RCATGY 2 cut(s) 163, 180
XmiI GTMKAC 1 cut(s) 363
XspI CTAG 6 cut(s) 57, 200, 272, 492, 947, 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.