MD16G1056100.v1.1

Belongs to the sulfotransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001120
Physical Location & Seq
Forward (+)
4007392 .. 4008390
999 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1056100.v1.1.491

Sequence Viewer

Length: 999 bp
ATGAAAGCACCTCAACCTCCTCCTCCTGATGTTCCCCGATACTTGCAAGAAAAGGAACACAGCCAAGAAGACAAAGACTTTATATTCTCTCTACCTACAGAAAAAGGTTGGGTTGCAGATGGCCTTCACAAGTACCATGGTTTTTGGCTGTCAACTAAGCAAATGCATGGAGTTTTGGCATGCCAGAAACACTTCCAAGCCGTAGATTCTGATATCGTTCTTGTTACCACTGTCAAATCAGGCACCACTTGGCTCAAGGCACTTATGTTTGCCCTTGTGAAACGAGCGCACTATCTGGACCCTCAGCGACACCCTTTGCTCACAGAAAATCCTCATGTTCTTGTGCCCTGTTTGGAGTTCGATGTCTACACCGACAAACAGGTACTTCCTGACCTCTCCTCCCTGTCCCGTCCAAGGCTCATTTCGACTCATCTACCGTATGTTTGTCTGCCGGATTCTGTCAAACATTCGGGTTGCAAAGTTGTTTACGCGTGGAGAGATCCTAAAGATGTGTTTGTTTCACTTTGGCACTTCTCGAACAAACTGAGGCCAGTGGGTAGTGGAACCATGTCACTTGACGAAGCGTTCGATAAGTTCTGCCGGGGAGTGAATACGTCCGGACCCTTTTGGGATCACGTACTCGGGTTTTGGAAGGAGAGCTTAAAGAGGCCTGAAAGTACATTTTTCATAAAGTATGAGGAAATGAAAGAAGAGCCCGCTCTTCACCTGAGGAGGCTGGCCGAGTTCTTGGGGTGCCCATTTTCCCCGGAAGAAGAGACACATGGAGTTGTGGACGGTATCTTAAGGTTGTGTAGTTTTGACAATTTAAGCAATTTGGTTGTGAATAAAAGTGGGAAATTGCCGGCCGGTATCGAGAATAGTGCCTTCTTTAGGAAAGGTGAAGTTGGAGATTGGATGAATAATTTGACCCCTGAGATGGTGAAAAAACTAGACTCCATTACTCAAGAGAAGTTGCATGGTTCCGGGCTAAAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sulfotransfer_1

Protein Analysis

333

Amino Acids

37.83

Weight (kDa)

6.95

Isoelectric Point (pI)

49.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sulfotransfer_1 PF00685 69 - 329 1.7e-71 Sulfotransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 242, 753
AccBSI CCGCTC 1 cut(s) 719
AccI GTMKAC 1 cut(s) 366
AccII CGCG 1 cut(s) 491
AccIII TCCGGA 1 cut(s) 617
AciI CCGC 1 cut(s) 717
AclWI GGATC 2 cut(s) 494, 639
AcoI YGGCCR 2 cut(s) 738, 864
AfaI GTAC 4 cut(s) 134, 384, 639, 679
AfiI CCNNNNNNNGG 3 cut(s) 414, 891, 937
AflII CTTAAG 1 cut(s) 802
AflIII ACRYGT 1 cut(s) 489
AjuI GAANNNNNNNTTGG 2 cut(s) 406, 438
AluBI AGCT 1 cut(s) 660
AluI AGCT 1 cut(s) 660
Alw26I GTCTC 1 cut(s) 770
AlwI GGATC 2 cut(s) 494, 639
Ama87I CYCGRG 1 cut(s) 641
Aor13HI TCCGGA 1 cut(s) 617
AoxI GGCC 5 cut(s) 121, 548, 668, 738, 864
ArsI GACNNNNNNTTYG 2 cut(s) 569, 601
Asp700I GAANNNNTTC 1 cut(s) 191
AspLEI GCGC 1 cut(s) 289
AspS9I GGNCC 2 cut(s) 298, 620
AsuC2I CCSGG 3 cut(s) 602, 767, 985
AsuHPI GGTGA 3 cut(s) 716, 911, 952
AvaI CYCGRG 1 cut(s) 641
AvaII GGWCC 2 cut(s) 298, 620
AxyI CCTNAGG 1 cut(s) 728
BaeGI GKGCMC 2 cut(s) 348, 758
BanI GGYRCC 2 cut(s) 242, 753
BanII GRGCYC 1 cut(s) 717
BbsI GAAGAC 1 cut(s) 75
BbvCI CCTCAGC 1 cut(s) 303
BccI CCATC 2 cut(s) 113, 931
BceAI ACGGC 1 cut(s) 185
BcgI CGANNNNNNTGC 2 cut(s) 863, 897
BcnI CCSGG 3 cut(s) 602, 767, 985
BcoDI GTCTC 1 cut(s) 770
BfaI CTAG 2 cut(s) 950, 997
BfmI CTRYAG 1 cut(s) 96
BfrI CTTAAG 1 cut(s) 802
Bme1390I CCNGG 3 cut(s) 602, 767, 985
Bme18I GGWCC 2 cut(s) 298, 620
BmeT110I CYCGRG 1 cut(s) 641
BmgT120I GGNCC 2 cut(s) 298, 620
BmiI GGNNCC 6 cut(s) 244, 300, 565, 622, 755, 982
BmrFI CCNGG 3 cut(s) 602, 767, 985
BpiI GAAGAC 1 cut(s) 75
Bpu10I CCTNAGC 1 cut(s) 303
BpuEI CTTGAG 2 cut(s) 239, 948
BpuMI CCSGG 3 cut(s) 602, 767, 985
BsaAI YACGTR 1 cut(s) 637
BsaJI CCNNGG 4 cut(s) 136, 413, 601, 765
BsaWI WCCGGW 1 cut(s) 617
BsaXI ACNNNNNCTCC 2 cut(s) 383, 413
Bsc4I CCNNNNNNNGG 3 cut(s) 414, 891, 937
Bse118I RCCGGY 2 cut(s) 862, 866
Bse1I ACTGG 1 cut(s) 551
Bse21I CCTNAGG 1 cut(s) 728
BseAI TCCGGA 1 cut(s) 617
BseDI CCNNGG 4 cut(s) 136, 413, 601, 765
BseGI GGATG 1 cut(s) 921
BseLI CCNNNNNNNGG 3 cut(s) 414, 891, 937
BseMII CTCAG 4 cut(s) 317, 536, 719, 924
BseNI ACTGG 1 cut(s) 551
BseRI GAGGAG 4 cut(s) 9, 12, 388, 745
BseSI GKGCMC 2 cut(s) 348, 758
BseX3I CGGCCG 1 cut(s) 864
Bsh1236I CGCG 1 cut(s) 491
Bsh1285I CGRYCG 1 cut(s) 867
BshFI GGCC 5 cut(s) 123, 550, 670, 740, 866
BshNI GGYRCC 2 cut(s) 242, 753
BsiEI CGRYCG 1 cut(s) 867
BsiHKCI CYCGRG 1 cut(s) 641
BsiSI CCGG 7 cut(s) 452, 601, 618, 767, 863, 867, 984
BslFI GGGAC 1 cut(s) 391
BslI CCNNNNNNNGG 3 cut(s) 414, 891, 937
BsmAI GTCTC 1 cut(s) 770
BsmFI GGGAC 1 cut(s) 391
BsnI GGCC 5 cut(s) 123, 550, 670, 740, 866
BsoBI CYCGRG 1 cut(s) 641
Bsp1286I GDGCHC 3 cut(s) 348, 717, 758
Bsp13I TCCGGA 1 cut(s) 617
Bsp143I GATC 2 cut(s) 499, 631
Bsp19I CCATGG 1 cut(s) 136
BspACI CCGC 1 cut(s) 717
BspANI GGCC 5 cut(s) 123, 550, 670, 740, 866
BspCNI CTCAG 4 cut(s) 316, 537, 720, 925
BspEI TCCGGA 1 cut(s) 617
BspFNI CGCG 1 cut(s) 491
BspLI GGNNCC 6 cut(s) 244, 300, 565, 622, 755, 982
BspPI GGATC 2 cut(s) 494, 639
BspQI GCTCTTC 2 cut(s) 705, 726
BspT107I GGYRCC 2 cut(s) 242, 753
BspTI CTTAAG 1 cut(s) 802
BsrBI CCGCTC 1 cut(s) 719
BsrFI RCCGGY 2 cut(s) 862, 866
BsrI ACTGG 1 cut(s) 551
BssAI RCCGGY 2 cut(s) 862, 866
BssECI CCNNGG 4 cut(s) 136, 413, 601, 765
BssMI GATC 2 cut(s) 499, 631
BssT1I CCWWGG 2 cut(s) 136, 413
Bst4CI ACNGT 3 cut(s) 232, 438, 797
Bst6I CTCTTC 3 cut(s) 705, 726, 768
BstAFI CTTAAG 1 cut(s) 802
BstBAI YACGTR 1 cut(s) 637
BstC8I GCNNGC 4 cut(s) 181, 717, 738, 864
BstDEI CTNAG 5 cut(s) 156, 303, 545, 728, 933
BstDSI CCRYGG 1 cut(s) 136
BstENI CCTNNNNNAGG 1 cut(s) 889
BstF5I GGATG 1 cut(s) 921
BstFNI CGCG 1 cut(s) 491
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 2 cut(s) 502, 634
BstMAI GTCTC 1 cut(s) 770
BstMBI GATC 2 cut(s) 499, 631
BstMCI CGRYCG 1 cut(s) 867
BstNSI RCATGY 1 cut(s) 183
BstSCI CCNGG 3 cut(s) 600, 765, 983
BstSFI CTRYAG 1 cut(s) 96
BstSLI GKGCMC 2 cut(s) 348, 758
BstUI CGCG 1 cut(s) 491
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
BstZI CGGCCG 1 cut(s) 864
Bsu36I CCTNAGG 1 cut(s) 728
BsuRI GGCC 5 cut(s) 123, 550, 670, 740, 866
BtgI CCRYGG 1 cut(s) 136
BtsCI GGATG 1 cut(s) 921
BtsIMutI CAGTG 2 cut(s) 228, 558
Cac8I GCNNGC 4 cut(s) 181, 717, 738, 864
CfoI GCGC 1 cut(s) 289
Cfr10I RCCGGY 2 cut(s) 862, 866
Cfr13I GGNCC 2 cut(s) 298, 620
Csp6I GTAC 4 cut(s) 133, 383, 638, 678
CviAII CATG 7 cut(s) 137, 167, 180, 335, 568, 782, 977
CviQI GTAC 4 cut(s) 133, 383, 638, 678
DdeI CTNAG 5 cut(s) 156, 303, 545, 728, 933
DpnI GATC 2 cut(s) 501, 633
DpnII GATC 2 cut(s) 499, 631
EaeI YGGCCR 2 cut(s) 738, 864
EagI CGGCCG 1 cut(s) 864
Eam1104I CTCTTC 3 cut(s) 705, 726, 768
EarI CTCTTC 3 cut(s) 705, 726, 768
EclXI CGGCCG 1 cut(s) 864
Eco130I CCWWGG 2 cut(s) 136, 413
Eco147I AGGCCT 1 cut(s) 670
Eco24I GRGCYC 1 cut(s) 717
Eco32I GATATC 1 cut(s) 214
Eco47I GGWCC 2 cut(s) 298, 620
Eco52I CGGCCG 1 cut(s) 864
Eco81I CCTNAGG 1 cut(s) 728
Eco88I CYCGRG 1 cut(s) 641
EcoNI CCTNNNNNAGG 1 cut(s) 889
EcoRV GATATC 1 cut(s) 214
EcoT14I CCWWGG 2 cut(s) 136, 413
EcoT22I ATGCAT 1 cut(s) 168
EcoT38I GRGCYC 1 cut(s) 717
ErhI CCWWGG 2 cut(s) 136, 413
FaeI CATG 7 cut(s) 140, 170, 183, 338, 571, 785, 980
FalI AAGNNNNNCTT 2 cut(s) 644, 676
FaqI GGGAC 1 cut(s) 391
FatI CATG 7 cut(s) 136, 166, 179, 334, 567, 781, 976
FauI CCCGC 1 cut(s) 724
FblI GTMKAC 1 cut(s) 366
FokI GGATG 1 cut(s) 928
FriOI GRGCYC 1 cut(s) 717
FspBI CTAG 2 cut(s) 950, 997
GlaI GCGC 1 cut(s) 288
HaeIII GGCC 5 cut(s) 123, 550, 670, 740, 866
HapII CCGG 7 cut(s) 452, 601, 618, 767, 863, 867, 984
HhaI GCGC 1 cut(s) 289
Hin1II CATG 7 cut(s) 140, 170, 183, 338, 571, 785, 980
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HincII GTYRAC 1 cut(s) 153
HindII GTYRAC 1 cut(s) 153
HinfI GANTC 4 cut(s) 206, 427, 455, 953
HpaII CCGG 7 cut(s) 452, 601, 618, 767, 863, 867, 984
HphI GGTGA 3 cut(s) 716, 911, 952
Hpy166II GTNNAC 4 cut(s) 153, 367, 487, 793
Hpy188I TCNGA 1 cut(s) 211
Hpy188III TCNNGA 7 cut(s) 26, 296, 389, 535, 618, 874, 965
Hpy8I GTNNAC 4 cut(s) 153, 367, 487, 793
HpyAV CCTTC 3 cut(s) 134, 646, 895
HpyCH4III ACNGT 3 cut(s) 232, 438, 797
HpyCH4IV ACGT 2 cut(s) 614, 636
HpyCH4V TGCA 5 cut(s) 46, 116, 166, 477, 976
HpyF3I CTNAG 5 cut(s) 156, 303, 545, 728, 933
HpySE526I ACGT 2 cut(s) 614, 636
Hsp92II CATG 7 cut(s) 140, 170, 183, 338, 571, 785, 980
HspAI GCGC 1 cut(s) 287
Kpn2I TCCGGA 1 cut(s) 617
KroI GCCGGC 1 cut(s) 862
KroNI GCCGGC 1 cut(s) 864
Kzo9I GATC 2 cut(s) 499, 631
LguI GCTCTTC 2 cut(s) 705, 726
MaeI CTAG 2 cut(s) 950, 997
MaeII ACGT 2 cut(s) 614, 636
MaeIII GTNAC 2 cut(s) 223, 570
MalI GATC 2 cut(s) 501, 633
MbiI CCGCTC 1 cut(s) 719
MboI GATC 2 cut(s) 499, 631
MboII GAAGA 5 cut(s) 80, 713, 722, 782, 785
MflI RGATCY 1 cut(s) 499
MhlI GDGCHC 3 cut(s) 348, 717, 758
MluCI AATT 4 cut(s) 823, 832, 857, 922
MluI ACGCGT 1 cut(s) 489
MlyI GAGTC 2 cut(s) 421, 947
MmeI TCCRAC 1 cut(s) 886
Mph1103I ATGCAT 1 cut(s) 168
MroI TCCGGA 1 cut(s) 617
MroNI GCCGGC 1 cut(s) 862
MroXI GAANNNNTTC 1 cut(s) 191
MseI TTAA 3 cut(s) 662, 803, 827
MspCI CTTAAG 1 cut(s) 802
MspI CCGG 7 cut(s) 452, 601, 618, 767, 863, 867, 984
MspR9I CCNGG 3 cut(s) 602, 767, 985
MvnI CGCG 1 cut(s) 491
NaeI GCCGGC 1 cut(s) 864
NciI CCSGG 3 cut(s) 602, 767, 985
NcoI CCATGG 1 cut(s) 136
NdeII GATC 2 cut(s) 499, 631
NgoMIV GCCGGC 1 cut(s) 862
NlaIII CATG 7 cut(s) 140, 170, 183, 338, 571, 785, 980
NlaIV GGNNCC 6 cut(s) 244, 300, 565, 622, 755, 982
NmeAIII GCCGAG 1 cut(s) 766
NmuCI GTSAC 1 cut(s) 570
NsiI ATGCAT 1 cut(s) 168
NspI RCATGY 1 cut(s) 183
PaeI GCATGC 1 cut(s) 183
PceI AGGCCT 1 cut(s) 670
PciSI GCTCTTC 2 cut(s) 705, 726
PcsI WCGNNNNNNNCGW 1 cut(s) 585
PdiI GCCGGC 1 cut(s) 864
PdmI GAANNNNTTC 1 cut(s) 191
PfeI GAWTC 2 cut(s) 206, 455
PleI GAGTC 2 cut(s) 421, 947
PpsI GAGTC 2 cut(s) 421, 947
Ppu21I YACGTR 1 cut(s) 637
PspN4I GGNNCC 6 cut(s) 244, 300, 565, 622, 755, 982
PspPI GGNCC 2 cut(s) 298, 620
PsuI RGATCY 1 cut(s) 499
RsaI GTAC 4 cut(s) 134, 384, 639, 679
RsaNI GTAC 4 cut(s) 133, 383, 638, 678
SapI GCTCTTC 2 cut(s) 705, 726
SaqAI TTAA 3 cut(s) 662, 803, 827
Sau3AI GATC 2 cut(s) 499, 631
Sau96I GGNCC 2 cut(s) 298, 620
SchI GAGTC 2 cut(s) 421, 947
ScrFI CCNGG 3 cut(s) 602, 767, 985
SduI GDGCHC 3 cut(s) 348, 717, 758
SfcI CTRYAG 1 cut(s) 96
SinI GGWCC 2 cut(s) 298, 620
SmlI CTYRAG 3 cut(s) 254, 802, 963
SmoI CTYRAG 3 cut(s) 254, 802, 963
SphI GCATGC 1 cut(s) 183
Sse9I AATT 4 cut(s) 823, 832, 857, 922
SseBI AGGCCT 1 cut(s) 670
SsiI CCGC 1 cut(s) 717
SspMI CTAG 2 cut(s) 950, 997
StuI AGGCCT 1 cut(s) 670
StyD4I CCNGG 3 cut(s) 600, 765, 983
StyI CCWWGG 2 cut(s) 136, 413
TaaI ACNGT 3 cut(s) 232, 438, 797
TaiI ACGT 2 cut(s) 617, 639
TaqI TCGA 5 cut(s) 360, 425, 536, 588, 873
TasI AATT 4 cut(s) 823, 832, 857, 922
TatI WGTACW 1 cut(s) 677
TfiI GAWTC 2 cut(s) 206, 455
Tru1I TTAA 3 cut(s) 662, 803, 827
Tru9I TTAA 3 cut(s) 662, 803, 827
TscAI CASTG 2 cut(s) 235, 558
TseFI GTSAC 1 cut(s) 570
Tsp45I GTSAC 1 cut(s) 570
TspDTI ATGAA 4 cut(s) 17, 676, 719, 932
TspRI CASTG 2 cut(s) 235, 558
Vha464I CTTAAG 1 cut(s) 802
VpaK11BI GGWCC 2 cut(s) 298, 620
XagI CCTNNNNNAGG 1 cut(s) 889
XceI RCATGY 1 cut(s) 183
XmiI GTMKAC 1 cut(s) 366
XmnI GAANNNNTTC 1 cut(s) 191
XspI CTAG 2 cut(s) 950, 997
Zsp2I ATGCAT 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.