MD17G1040000.v1.1

Senescence-associated carboxylesterase 101-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000388
Physical Location & Seq
Forward (+)
2926454 .. 2927272
819 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1040000.v1.1.491

Sequence Viewer

Length: 819 bp
ATGGTAACCAAAGAATGTAAACAAGCGATCGACAAGGCGAAGGCTTACAGTTCTTTCTTGAAATTGAATGAGAATAGAGAGTACATGGCCAGCATGGAATGGTACAGGAAGGAGTCCAAAGAGATGGGAATTGGATACTATGACAGGTACAGAAACAAGCTTTACTTAAGTGACATTATGGCCACAGATTACAAGAAGAAGCTCTCGAACTACTGGGAGGACACGGTTGCAGAAGTTGAGAGCAATCCCCAGAAAGAAGGAGCCGCAATGCGTACTCGTTTCCTATTTGGTGGAACGAATTACAGAAGGATGATCGAACCGCTTCACATTGCCGAGTACTACAAGGAAGGTGGAAAAGATTACATAAAGGAAAGGCCTCGACATTTCATTCTGTTGGAGCAATGGTTCAATGAAGACGAGGAAAAGAAGAAGGCAGAGAGAGAGAAGAAAGAAAGGGAGAACCCCCAACTGCGCAGCGATACCAAGTCGAACTCAAAAGCGAAGAATGTGGCTTCTAGTCTCAATGATGATTCTTGTTTTTGCGTGCACGTTGAGGAAGCGCTTATCTTGTACAATGAACAAGCGAGTAATCCAGATGCCAAGCAAAAGTTGATTGACTTTGAGCATTACGTGCTGAATAATCTCGAGAAGTTTGCAGTGACGCTTGATATTTTCTTGGCCCAAAGCAGCTACATGCAGTGGTGGAACAAGTACGAAAAAAGGGTGGGAAATGACTATTCCTCACCACTGGCCAAGGTCATGAAGCGTCGCACTTATACCAAGTATGCAGAGGGGGTCTCGGTTCTTGCTGATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

273

Amino Acids

32.26

Weight (kDa)

8.45

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDS1_EP PF18117 30 - 252 9.5e-58 Enhanced disease susceptibility 1 protein EP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000388)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14930 AT5G14930 AT5G14930
fragaria_vesca FvH4_2g18461 FvH4_6g50160 FvH4_6g50160 FvH4_6g50160 FvH4_6g50162
malus_domestica MD01G1017800.v1.1 MD09G1038500.v1.1 MD09G1038700.v1.1 MD09G1039000.v1.1 MD09G1039700.v1.1 MD09G1039800.v1.1 MD17G1039600.v1.1 MD17G1039700.v1.1 MD17G1039800.v1.1 MD17G1039900.v1.1 MD17G1040000.v1.1
prunus_persica Prupe.2G019500_v2.0.a1 Prupe.3G279000_v2.0.a1 Prupe.3G279000_v2.0.a1 Prupe.3G279000_v2.0.a1 Prupe.3G279300_v2.0.a1
pyrus_communis pycom111g03070 pycom17g03570 pycom17g03580
rosa_chinensis RchiOBHm_Chr2g0170411 RchiOBHm_Chr2g0170431 RchiOBHm_Chr2g0170561 RchiOBHm_Chr6g0283951 RchiOBHm_Chr6g0283961 RchiOBHm_Chr6g0283981 RchiOBHm_Chr7g0219521 RchiOBHm_Chr7g0219541
rosa_laevigata RLG00000002285 RLG00000012782 RLG00000012784 RLG00000021954 RLG00000021955 RLG00000021956
rosa_multiflora Rmu_co8245087.1_g000001 Rmu_co8259259.1_g000001 Rmu_sc0003047.1_g000001 Rmu_sc0008697.1_g000004 Rmu_sc0009395.1_g000014 Rmu_sc0009395.1_g000016 Rmu_sc0009395.1_g000019 Rmu_sc0009395.1_g000026 Rmu_sc0009395.1_g000028 Rmu_sc0009395.1_g000040 Rmu_sc0021809.1_g000001 Rmu_ssc0000442.1_g000026 Rmu_ssc0000442.1_g000030
rosa_roxburghii Rroxscaffold_2G00081280 Rroxscaffold_2G00081290 Rroxscaffold_2G00081330 Rroxscaffold_3G00239990 Rroxscaffold_4G00294050 Rroxscaffold_4G00294070 Rroxscaffold_7G00185030
rosa_rugosa Rorug02G0548700 Rorug02G0548900 Rorug06G0158800 Rorug06G0158900 Rorug07G0186000
rosa_samantha Rh2AG620300 Rh2AG620500 Rh2AG620600 Rh2AG620700 Rh2BG631200 Rh2BG631400 Rh2BG631600 Rh2BG631700 Rh2CG601100 Rh2CG601200 Rh2CG601300 Rh2DG643900 Rh2DG644200 Rh2DG644400 Rh6AG272000 Rh6AG272300 Rh6BG272900 Rh6BG273000 Rh6CG274100 Rh6CG274200 Rh6DG267100 Rh6DG267200 Rh6DG267300 Rh7AG329500 Rh7AG329900 Rh7BG319400 Rh7BG319600 Rh7CG346800 Rh7CG347200 Rh7DG325400
rosa_wichuraiana Rw2G051410 Rw2G051430 Rw2G051440 Rw2G051450 Rw6G023440 Rw7G027780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 473
AciI CCGC 2 cut(s) 264, 320
AcoI YGGCCR 3 cut(s) 87, 180, 750
AfaI GTAC 7 cut(s) 83, 104, 149, 274, 338, 572, 713
AfeI AGCGCT 1 cut(s) 561
AflII CTTAAG 1 cut(s) 166
AgsI TTSAA 3 cut(s) 61, 67, 409
AloI GAACNNNNNNTCC 2 cut(s) 389, 421
AluBI AGCT 3 cut(s) 160, 202, 690
AluI AGCT 3 cut(s) 160, 202, 690
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 2 cut(s) 524, 802
Alw44I GTGCAC 1 cut(s) 545
Ama87I CYCGRG 1 cut(s) 644
Aor51HI AGCGCT 1 cut(s) 561
AoxI GGCC 5 cut(s) 87, 180, 374, 678, 750
ApaLI GTGCAC 1 cut(s) 545
ApeKI GCWGC 2 cut(s) 474, 687
Asp700I GAANNNNTTC 1 cut(s) 321
AspLEI GCGC 2 cut(s) 474, 562
AspS9I GGNCC 1 cut(s) 679
AsuHPI GGTGA 1 cut(s) 735
AvaI CYCGRG 1 cut(s) 644
BaeGI GKGCMC 1 cut(s) 549
BalI TGGCCA 3 cut(s) 89, 182, 752
BbsI GAAGAC 1 cut(s) 420
Bbv12I GWGCWC 1 cut(s) 549
BbvI GCAGC 2 cut(s) 486, 699
BccI CCATC 1 cut(s) 118
BcgI CGANNNNNNTGC 2 cut(s) 635, 669
BciVI GTATCC 1 cut(s) 128
BcoDI GTCTC 2 cut(s) 524, 802
BfaI CTAG 1 cut(s) 516
BfoI RGCGCY 1 cut(s) 563
BfrI CTTAAG 1 cut(s) 166
BfuI GTATCC 1 cut(s) 128
BisI GCNGC 3 cut(s) 264, 475, 688
BlsI GCNGC 3 cut(s) 265, 476, 689
BmcAI AGTACT 1 cut(s) 338
BmeT110I CYCGRG 1 cut(s) 644
BmgT120I GGNCC 1 cut(s) 679
BmiI GGNNCC 1 cut(s) 262
BmrI ACTGGG 1 cut(s) 223
BmsI GCATC 1 cut(s) 586
BmuI ACTGGG 1 cut(s) 223
BpiI GAAGAC 1 cut(s) 420
BplI GAGNNNNNCTC 2 cut(s) 782, 814
BsaAI YACGTR 1 cut(s) 631
BsaI GGTCTC 1 cut(s) 802
BsaJI CCNNGG 1 cut(s) 753
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bse1I ACTGG 2 cut(s) 218, 753
Bse3DI GCAATG 3 cut(s) 273, 327, 407
BseDI CCNNGG 1 cut(s) 753
BseGI GGATG 1 cut(s) 315
BseMI GCAATG 3 cut(s) 273, 327, 407
BseNI ACTGG 2 cut(s) 218, 753
BseSI GKGCMC 1 cut(s) 549
BseXI GCAGC 2 cut(s) 486, 699
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 5 cut(s) 89, 182, 376, 680, 752
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 549
BsiHKCI CYCGRG 1 cut(s) 644
BsmAI GTCTC 2 cut(s) 524, 802
BsnI GGCC 5 cut(s) 89, 182, 376, 680, 752
Bso31I GGTCTC 1 cut(s) 802
BsoBI CYCGRG 1 cut(s) 644
Bsp1286I GDGCHC 1 cut(s) 549
Bsp1407I TGTACA 1 cut(s) 570
Bsp143I GATC 2 cut(s) 27, 312
BspACI CCGC 2 cut(s) 264, 320
BspANI GGCC 5 cut(s) 89, 182, 376, 680, 752
BspHI TCATGA 1 cut(s) 759
BspLI GGNNCC 1 cut(s) 262
BspTI CTTAAG 1 cut(s) 166
BspTNI GGTCTC 1 cut(s) 802
BsrDI GCAATG 3 cut(s) 273, 327, 407
BsrGI TGTACA 1 cut(s) 570
BsrI ACTGG 2 cut(s) 218, 753
BssECI CCNNGG 1 cut(s) 753
BssMI GATC 2 cut(s) 27, 312
BssT1I CCWWGG 1 cut(s) 753
Bst4CI ACNGT 2 cut(s) 50, 226
BstAFI CTTAAG 1 cut(s) 166
BstAPI GCANNNNNTGC 1 cut(s) 631
BstAUI TGTACA 1 cut(s) 570
BstBAI YACGTR 1 cut(s) 631
BstC8I GCNNGC 2 cut(s) 91, 545
BstEII GGTNACC 1 cut(s) 4
BstF5I GGATG 1 cut(s) 315
BstH2I RGCGCY 1 cut(s) 563
BstHHI GCGC 2 cut(s) 474, 562
BstKTI GATC 2 cut(s) 30, 315
BstMAI GTCTC 2 cut(s) 524, 802
BstMBI GATC 2 cut(s) 27, 312
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 1 cut(s) 631
BstNSI RCATGY 1 cut(s) 697
BstPI GGTNACC 1 cut(s) 4
BstSLI GKGCMC 1 cut(s) 549
BstV1I GCAGC 2 cut(s) 486, 699
BstV2I GAAGAC 1 cut(s) 420
BstXI CCANNNNNNTGG 1 cut(s) 124
BsuI GTATCC 1 cut(s) 128
BsuRI GGCC 5 cut(s) 89, 182, 376, 680, 752
BtsCI GGATG 1 cut(s) 315
BtsI GCAGTG 2 cut(s) 663, 704
BtsIMutI CAGTG 3 cut(s) 663, 704, 746
Cac8I GCNNGC 2 cut(s) 91, 545
CciI TCATGA 1 cut(s) 759
CfoI GCGC 2 cut(s) 474, 562
Cfr13I GGNCC 1 cut(s) 679
CseI GACGC 2 cut(s) 670, 755
Csp6I GTAC 7 cut(s) 82, 103, 148, 273, 337, 571, 712
CspCI CAANNNNNGTGG 2 cut(s) 331, 366
CviAII CATG 4 cut(s) 85, 94, 694, 760
CviQI GTAC 7 cut(s) 82, 103, 148, 273, 337, 571, 712
DpnI GATC 2 cut(s) 29, 314
DpnII GATC 2 cut(s) 27, 312
EaeI YGGCCR 3 cut(s) 87, 180, 750
Eco130I CCWWGG 1 cut(s) 753
Eco147I AGGCCT 1 cut(s) 376
Eco31I GGTCTC 1 cut(s) 802
Eco47III AGCGCT 1 cut(s) 561
Eco88I CYCGRG 1 cut(s) 644
Eco91I GGTNACC 1 cut(s) 4
EcoO65I GGTNACC 1 cut(s) 4
EcoT14I CCWWGG 1 cut(s) 753
ErhI CCWWGG 1 cut(s) 753
FaeI CATG 4 cut(s) 88, 97, 697, 763
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FatI CATG 4 cut(s) 84, 93, 693, 759
Fnu4HI GCNGC 3 cut(s) 264, 475, 688
FokI GGATG 1 cut(s) 322
Fsp4HI GCNGC 3 cut(s) 264, 475, 688
FspBI CTAG 1 cut(s) 516
FspI TGCGCA 1 cut(s) 473
GlaI GCGC 2 cut(s) 473, 561
GluI GCNGC 3 cut(s) 264, 475, 688
HaeII RGCGCY 1 cut(s) 563
HaeIII GGCC 5 cut(s) 89, 182, 376, 680, 752
HgaI GACGC 2 cut(s) 670, 755
HhaI GCGC 2 cut(s) 474, 562
Hin1II CATG 4 cut(s) 88, 97, 697, 763
Hin6I GCGC 2 cut(s) 472, 560
HinP1I GCGC 2 cut(s) 472, 560
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 2 cut(s) 113, 530
HphI GGTGA 1 cut(s) 735
Hpy166II GTNNAC 2 cut(s) 20, 547
Hpy188III TCNNGA 6 cut(s) 58, 205, 593, 644, 646, 760
Hpy8I GTNNAC 2 cut(s) 20, 547
Hpy99I CGWCG 1 cut(s) 771
HpyAV CCTTC 6 cut(s) 34, 103, 251, 300, 341, 424
HpyCH4III ACNGT 2 cut(s) 50, 226
HpyCH4IV ACGT 2 cut(s) 549, 630
HpyCH4V TGCA 5 cut(s) 230, 547, 656, 697, 788
HpyF10VI GCNNNNNNNGC 1 cut(s) 631
HpySE526I ACGT 2 cut(s) 549, 630
Hsp92II CATG 4 cut(s) 88, 97, 697, 763
HspAI GCGC 2 cut(s) 472, 560
Kzo9I GATC 2 cut(s) 27, 312
LmnI GCTCC 2 cut(s) 260, 397
LpnPI CCDG 7 cut(s) 91, 103, 130, 199, 263, 606, 734
Lsp1109I GCAGC 2 cut(s) 486, 699
LweI GCATC 1 cut(s) 586
MaeI CTAG 1 cut(s) 516
MaeII ACGT 2 cut(s) 549, 630
MaeIII GTNAC 3 cut(s) 4, 170, 658
MalI GATC 2 cut(s) 29, 314
MboI GATC 2 cut(s) 27, 312
MboII GAAGA 5 cut(s) 208, 425, 439, 457, 514
MhlI GDGCHC 1 cut(s) 549
MlsI TGGCCA 3 cut(s) 89, 182, 752
MluCI AATT 3 cut(s) 62, 129, 298
MluNI TGGCCA 3 cut(s) 89, 182, 752
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 1 cut(s) 375
MnlI CCTC 6 cut(s) 211, 387, 412, 547, 751, 784
Mox20I TGGCCA 3 cut(s) 89, 182, 752
MroXI GAANNNNTTC 1 cut(s) 321
MscI TGGCCA 3 cut(s) 89, 182, 752
MseI TTAA 1 cut(s) 167
Msp20I TGGCCA 3 cut(s) 89, 182, 752
MspCI CTTAAG 1 cut(s) 166
MwoI GCNNNNNNNGC 1 cut(s) 631
NdeII GATC 2 cut(s) 27, 312
NlaIII CATG 4 cut(s) 88, 97, 697, 763
NlaIV GGNNCC 1 cut(s) 262
NmeAIII GCCGAG 1 cut(s) 358
NmuCI GTSAC 2 cut(s) 170, 658
NsbI TGCGCA 1 cut(s) 473
NspI RCATGY 1 cut(s) 697
PaeR7I CTCGAG 1 cut(s) 644
PagI TCATGA 1 cut(s) 759
PceI AGGCCT 1 cut(s) 376
PdmI GAANNNNTTC 1 cut(s) 321
PfeI GAWTC 1 cut(s) 530
PkrI GCNGC 3 cut(s) 265, 476, 689
Ple19I CGATCG 1 cut(s) 30
PleI GAGTC 1 cut(s) 121
PpsI GAGTC 1 cut(s) 121
Ppu21I YACGTR 1 cut(s) 631
PspEI GGTNACC 1 cut(s) 4
PspN4I GGNNCC 1 cut(s) 262
PspPI GGNCC 1 cut(s) 679
PvuI CGATCG 1 cut(s) 30
RsaI GTAC 7 cut(s) 83, 104, 149, 274, 338, 572, 713
RsaNI GTAC 7 cut(s) 82, 103, 148, 273, 337, 571, 712
SaqAI TTAA 1 cut(s) 167
SatI GCNGC 3 cut(s) 264, 475, 688
Sau3AI GATC 2 cut(s) 27, 312
Sau96I GGNCC 1 cut(s) 679
ScaI AGTACT 1 cut(s) 338
SchI GAGTC 1 cut(s) 122
SduI GDGCHC 1 cut(s) 549
SetI ASST 8 cut(s) 149, 162, 204, 352, 552, 633, 692, 759
SfaNI GCATC 1 cut(s) 586
Sfr274I CTCGAG 1 cut(s) 644
SlaI CTCGAG 1 cut(s) 644
SmlI CTYRAG 2 cut(s) 166, 644
SmoI CTYRAG 2 cut(s) 166, 644
Sse9I AATT 3 cut(s) 62, 129, 298
SseBI AGGCCT 1 cut(s) 376
SsiI CCGC 2 cut(s) 264, 320
SspMI CTAG 1 cut(s) 516
StuI AGGCCT 1 cut(s) 376
StyI CCWWGG 1 cut(s) 753
TaaI ACNGT 2 cut(s) 50, 226
TaiI ACGT 2 cut(s) 552, 633
TaqI TCGA 6 cut(s) 30, 206, 315, 379, 488, 645
TasI AATT 3 cut(s) 62, 129, 298
TatI WGTACW 3 cut(s) 81, 336, 570
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 1 cut(s) 530
Tru1I TTAA 1 cut(s) 167
Tru9I TTAA 1 cut(s) 167
TscAI CASTG 3 cut(s) 663, 704, 753
TseFI GTSAC 2 cut(s) 170, 658
TseI GCWGC 2 cut(s) 474, 687
Tsp45I GTSAC 2 cut(s) 170, 658
TspDTI ATGAA 4 cut(s) 376, 426, 591, 776
TspRI CASTG 3 cut(s) 663, 704, 753
Vha464I CTTAAG 1 cut(s) 166
VneI GTGCAC 1 cut(s) 545
XceI RCATGY 1 cut(s) 697
XhoI CTCGAG 1 cut(s) 644
XmnI GAANNNNTTC 1 cut(s) 321
XspI CTAG 1 cut(s) 516
ZrmI AGTACT 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.