Rh7DG325400

GPI ethanolamine phosphate transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000388
Physical Location & Seq
Reverse (-)
39215206 .. 39224220
9015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG325400.1

Sequence Viewer

Length: 3567 bp
ATGCTCGGCCGCCGACTCAGTCGAGGCCGGGACACGTGGCGTTTATTGCTGGCTTCTATGAGGATCCGAGTGCTGTTACGAATGGGTGGAAAGCTAATCCTGTTGAATTTGATTCGTTATCCTCATGATTTCGAAGACTTTGCTACTGATGCCTCCTTTCTGGATGAGTGGTCGTTTGATCAATTTCAGAGCCTAATGAATAGGTCCAAAGAGGACCCAAAGTTGAAGGAGTTACTTGTACAGGATAATCTTGTTGTGTTTTTGCACCTACTTGGTTGTGATTCAAATGGTTGGAAGCTTTTGCTAGAGAAGTGCATGACCAGTAGCCACGGAGATGGACATCCTTCAAACACTGATACTCCCCTTGTTGTTTGGGGAGCAGGTGTTAAACACCCAAAGCCTATTTCCAGCAGCAGCCATGGAGATGGACATTCTGATTGTGGTACTCGTTTTTTTGATGACCATATGCATGACACGCCAACGCCTAAAGAATGGCGCCTTCATGGCATTGAAAGGGTGGATGTCAATCAAGCTGATATCGCACCTCTCATGTCAACACTTCTTGGATTGCCATGTCCTGTAAACTCGGTCGGGAGTTTACCCCTTGATTACATAGACATGAAAAAGACAGATGAAGTTGAAGCTGTTCTAGCCAATACAAAGCAAGTTCTCAATCAATTTGTTCGGAAATCACAAACCAAGGAGGCAACTTCATTATATTTTAAGCCTTTCAAACCATTGGGCCATTATTCAACCCTCTTGGATCAGATTGAGGATCATATATCTAATGGAGACTATGAAGCTGCAAGGAAACTATCTGAAAATCTCCGAGACTTGGGGCTGCAAGGACTTCATTATTTTCAAACTTATGATTGGCTGATGCTCATGACTGTAATTTTTCTTGGGTATATTGGTTGGATGACCTATATTGTTGTCCATGTGTTGCAATCTTACATGTCATTGGCAGGGGATATATTGACAAAGGAGCAAGCAGATCACTGGACAGATTATACCAGAAAAGTACAACTTTGTGGATGCTTGTTTCTGGGACTACTTAGCATCATACTGTTCATGGAGCAGGCTCCTCCTCTTTACCATGCATACACAACAATGACAGTATTTTTGTGGACACAAATATCTAGTGAATATCGGTTCATAAAAGCATTATGGAAACAGTTACATGGAAGAGAAATAAATTATTTCACAAAAGTTGGAGCCGCCTGTGCTGTCTCAGTATTCATACTTGAATATCTGGTGAACAGCTTCACCGAGAGGAAGCTCTACACATGGTCGGAAATTCCGGATAATAATAATTTAGTAATTTGGAATGCAAAGAAATCCAAGTTCCCCATGTTCTTTCAATTACAGGTTCTTTTGGTTGGATTATCATCTGTGATGGTGTCAATATCAACATCTCATAGAACAGAGAACCAAGAATTGCATGTCTGGCACCAGATTATAAATTGGTCCATTGCCGGTCCCTCGATGGTACTTCCATTATTTTCAGTAAATGGTCTCTTGTCCCGGCTTACGTCCATATTTCTTGGCTTTGCGCCCACATTTCTTCTATTATCTATCGGATATGAAGCTGTCTTCTATGGTGCTTTTGCTCTTGTACTCATGGCTTGGATACTATTTGAAAACACTCTTATTTACTCAAGTAAGCTGTCTGACATCAGAATTCCTTTAATATTTATGGTCTTGTTTAATGTTGCCTTCGGAACGGGAAATTTTGCTAGTATCGCAAGTTTCGAAATCTCATCAGTCTATCGGTTCATTACTGTCTTCAGCCCTTTTCTTATGGCAGTGCTACTCATCTTCAAGCTATTCATTCCATTTCTGCTTGTCATGTTTAGCAGCGGCTTAGAATCGGGGAATTTTGTGGTGAACTCTGATCCGGTACACCAGGCATGGTGTGCGATTGAGCAACAAAGACAGGTTAATCCAAATGCAGAGCCATCCTTGTACAATGAAATAATCCAACCAGAAAATTCAATCGTCATAGCTTTTGGCACTCCACCTGGCTCTCTTCAGGGACAAGAAGGCTTGGTTTCGTCAGAAGATCCTCACTTTGAATTTTTGTGCAACAAAAGCAATCCTGTTTTCTCCATCAATGAAGCAGCTATCAAACTATTTCAGTCGCATTATAATGATCTCCTCCTCCTGAAAAATAAGCTGGTAGAGAAAAGCAAGAGCAAAACCCCACCATTAATAATCATCACTGGACAATTTTTGGGAGGTAGTGTGGCTACACTCTTCACCTTATGGTTGCTAGAAGGCCTCAACTTGTCGAAAACCAAACGCCCGCTTTGCATTACTTTCGGTTCTCCACTTGTTGGCGATGAACACCTTCGAAAATGTGTGCTAGAATTCTCAACTTGGAAGTCTTGCTTCTTGCACATAGTCTCCGACCAAGATCATACACCGAAAATCTTTATGTCCCAAAATACAACTGGTGCTTATAAGCCGTTTGGAACATTCTTGTTGTGCTCGGCTTCGGGTTGTGCTTGCTCTGAGGACCCGGATTTCATTTCGGAACAATTTGTGACAACCAATTCTCCCAGTGCTCAAACTCAAGATCCTAATTTGGGGTTCTCTTATGGACAAATTTTGGAGGATCTCAAGCGGAAGGCATTATGTAATATATTCAAGTCCATTGAAGGGCAAAGCCATCCGCTTCAAGCTAGCATAATCACACAACTCCTAGCAATTGGAGTAGTCTCACAGCAACAGTCCCAGGGTACTGACAATCTGGTCAGGAAGATGAAAAAACATGAAACAAAGTTATTAATTCAGAAGAAGAAGTCAGATTCTGACAAGAAATTGAATGAAATGAAAATTCAAATGGCCTTCTTGGAGTGGTACAAGAAGGACGCCAAACGTCAAAATATTGGATACTATGACTTGTACAGAAAGATGGGCAATCAATCTGACTATAAGGTTAACGAGTATAAGAAGAAGCTCATGAATTACTGGGAGGACTCTGTCACAGAAGTAGAGAACAAGCCCCAGTTAGAAGGAGCTCACTTTCGGGTTCGTTGGCTTTATGCAGGCACAAACTACAGAGGAATGGTTGAACCACTTCACATTGCAGACTACTATAGGGATGGGGGTAAAAATTACCATACTGATGCTGGGAAAAGGCCTAAACATTTTACTCTGTTGGAGGAATGGCATCAGGAGAAGCAGGAGCAAGAAAAGAAGAAGAAGCAGGAAACACAGAAACAAGAAGAAAAACCAGAATCTGGCCCAAGCAAATCCAAAAGAGAGAACGTGGGTTCTAGTTTGAATGATGATTTTTGTTTCTGGGCGCGTGTTGAGGAAGCTCTCATCTTGTTGGAGAATGGAGGACTAACTACTGATGACAAAAGGAAGTTGAAAGAGTTTGAGGACTACGTGTGGAATGCTCTCAAGAATTATGCAGTGTCACCTGAGATTTTCTTGAAGAAGAGCAGTTTTATGAAATGGTGGAATGAGTATAAGGGAATTGTTGAAAGCTCGTCATTGCTCTTGGACTTCATGAAAAATGGCGGTCCCAGAGAGTACGAGGCAGGGAAGTTCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Phosphodiest PigN Sulfatase

Protein Analysis

1188

Amino Acids

135.7

Weight (kDa)

6.92

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PigN PF04987 282 - 430 5.8e-30 Phosphatidylinositolglycan class N (PIG-N)
PigN PF04987 445 - 619 1e-49 Phosphatidylinositolglycan class N (PIG-N)
Lipase_3 PF01764 701 - 813 6.5e-12 Lipase (class 3)
EDS1_EP PF18117 952 - 1167 6.2e-59 Enhanced disease susceptibility 1 protein EP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000388)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14930 AT5G14930 AT5G14930
fragaria_vesca FvH4_2g18461 FvH4_6g50160 FvH4_6g50160 FvH4_6g50160 FvH4_6g50162
malus_domestica MD01G1017800.v1.1 MD09G1038500.v1.1 MD09G1038700.v1.1 MD09G1039000.v1.1 MD09G1039700.v1.1 MD09G1039800.v1.1 MD17G1039600.v1.1 MD17G1039700.v1.1 MD17G1039800.v1.1 MD17G1039900.v1.1 MD17G1040000.v1.1
prunus_persica Prupe.2G019500_v2.0.a1 Prupe.3G279000_v2.0.a1 Prupe.3G279000_v2.0.a1 Prupe.3G279000_v2.0.a1 Prupe.3G279300_v2.0.a1
pyrus_communis pycom111g03070 pycom17g03570 pycom17g03580
rosa_chinensis RchiOBHm_Chr2g0170411 RchiOBHm_Chr2g0170431 RchiOBHm_Chr2g0170561 RchiOBHm_Chr6g0283951 RchiOBHm_Chr6g0283961 RchiOBHm_Chr6g0283981 RchiOBHm_Chr7g0219521 RchiOBHm_Chr7g0219541
rosa_laevigata RLG00000002285 RLG00000012782 RLG00000012784 RLG00000021954 RLG00000021955 RLG00000021956
rosa_multiflora Rmu_co8245087.1_g000001 Rmu_co8259259.1_g000001 Rmu_sc0003047.1_g000001 Rmu_sc0008697.1_g000004 Rmu_sc0009395.1_g000014 Rmu_sc0009395.1_g000016 Rmu_sc0009395.1_g000019 Rmu_sc0009395.1_g000026 Rmu_sc0009395.1_g000028 Rmu_sc0009395.1_g000040 Rmu_sc0021809.1_g000001 Rmu_ssc0000442.1_g000026 Rmu_ssc0000442.1_g000030
rosa_roxburghii Rroxscaffold_2G00081280 Rroxscaffold_2G00081290 Rroxscaffold_2G00081330 Rroxscaffold_3G00239990 Rroxscaffold_4G00294050 Rroxscaffold_4G00294070 Rroxscaffold_7G00185030
rosa_rugosa Rorug02G0548700 Rorug02G0548900 Rorug06G0158800 Rorug06G0158900 Rorug07G0186000
rosa_samantha Rh2AG620300 Rh2AG620500 Rh2AG620600 Rh2AG620700 Rh2BG631200 Rh2BG631400 Rh2BG631600 Rh2BG631700 Rh2CG601100 Rh2CG601200 Rh2CG601300 Rh2DG643900 Rh2DG644200 Rh2DG644400 Rh6AG272000 Rh6AG272300 Rh6BG272900 Rh6BG273000 Rh6CG274100 Rh6CG274200 Rh6DG267100 Rh6DG267200 Rh6DG267300 Rh7AG329500 Rh7AG329900 Rh7BG319400 Rh7BG319600 Rh7CG346800 Rh7CG347200 Rh7DG325400
rosa_wichuraiana Rw2G051410 Rw2G051430 Rw2G051440 Rw2G051450 Rw6G023440 Rw7G027780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1460, 2148, 2463
AarI CACCTGC 1 cut(s) 371
AasI GACNNNNNNGTC 1 cut(s) 2753
Acc36I ACCTGC 1 cut(s) 371
AccB1I GGYRCC 2 cut(s) 495, 1449
AccB7I CCANNNNNTGG 2 cut(s) 2336, 3245
AccII CGCG 1 cut(s) 3313
AccIII TCCGGA 1 cut(s) 1300
AciI CCGC 7 cut(s) 10, 1218, 1860, 2306, 2626, 2675, 3531
AclWI GGATC 8 cut(s) 58, 71, 771, 783, 1889, 2057, 2573, 2625
AcoI YGGCCR 1 cut(s) 7
AcuI CTGAAG 2 cut(s) 1771, 2015
AcvI CACGTG 1 cut(s) 36
AcyI GRCGYC 2 cut(s) 496, 2874
AfiI CCNNNNNNNGG 5 cut(s) 835, 2336, 2588, 2661, 3245
AflIII ACRYGT 3 cut(s) 33, 954, 3396
AjnI CCWGG 3 cut(s) 1905, 2020, 2736
AjuI GAANNNNNNNTTGG 2 cut(s) 388, 420
Alw21I GWGCWC 3 cut(s) 2492, 2569, 3025
Alw26I GTCTC 6 cut(s) 786, 825, 1234, 1520, 2410, 2725
AlwI GGATC 8 cut(s) 58, 71, 771, 783, 1889, 2057, 2573, 2625
AlwNI CAGNNNCTG 2 cut(s) 2813, 3245
Aor13HI TCCGGA 1 cut(s) 1300
AoxI GGCC 7 cut(s) 7, 25, 742, 2278, 2847, 3143, 3247
ApeKI GCWGC 6 cut(s) 411, 414, 803, 841, 1857, 2120
ArsI GACNNNNNNTTYG 2 cut(s) 1105, 1137
AseI ATTAAT 2 cut(s) 2210, 2789
Asp700I GAANNNNTTC 4 cut(s) 645, 1262, 2349, 3081
AspLEI GCGC 3 cut(s) 498, 1555, 3313
AspS9I GGNCC 8 cut(s) 204, 214, 742, 1467, 1478, 2518, 3248, 3533
AsuC2I CCSGG 3 cut(s) 29, 1525, 2522
AsuHPI GGTGA 5 cut(s) 1258, 1267, 1897, 2251, 3420
AsuII TTCGAA 3 cut(s) 132, 1752, 2353
AsuNHI GCTAGC 1 cut(s) 2684
AvaII GGWCC 6 cut(s) 204, 214, 1467, 1478, 2518, 3533
BamHI GGATCC 1 cut(s) 63
BanI GGYRCC 2 cut(s) 495, 1449
BanII GRGCYC 1 cut(s) 3025
BbrPI CACGTG 1 cut(s) 36
BbsI GAAGAC 3 cut(s) 141, 1585, 1777
Bbv12I GWGCWC 3 cut(s) 2492, 2569, 3025
BbvI GCAGC 6 cut(s) 423, 426, 790, 828, 1869, 2132
BccI CCATC 9 cut(s) 329, 419, 1390, 1480, 1966, 2117, 2679, 2911, 3101
BceAI ACGGC 1 cut(s) 2452
BcgI CGANNNNNNTGC 4 cut(s) 122, 156, 2302, 2336
BciT130I CCWGG 3 cut(s) 1907, 2022, 2738
BciVI GTATCC 2 cut(s) 1623, 2888
BclI TGATCA 1 cut(s) 178
BcnI CCSGG 3 cut(s) 29, 1525, 2522
BcoDI GTCTC 6 cut(s) 786, 825, 1234, 1520, 2410, 2725
BfaI CTAG 9 cut(s) 305, 650, 1140, 1737, 2273, 2366, 2685, 2705, 3282
BfmI CTRYAG 2 cut(s) 3061, 3100
BfoI RGCGCY 1 cut(s) 499
BfuAI ACCTGC 1 cut(s) 371
BfuI GTATCC 2 cut(s) 1623, 2888
BglI GCCNNNNNGGC 1 cut(s) 504
BisI GCNGC 9 cut(s) 10, 412, 415, 804, 842, 1218, 1858, 1861, 2121
BlsI GCNGC 9 cut(s) 11, 413, 416, 805, 843, 1219, 1859, 1862, 2122
Bme1390I CCNGG 6 cut(s) 29, 1525, 1907, 2022, 2522, 2738
Bme18I GGWCC 6 cut(s) 204, 214, 1467, 1478, 2518, 3533
BmgT120I GGNCC 8 cut(s) 204, 214, 742, 1467, 1478, 2518, 3248, 3533
BmiI GGNNCC 9 cut(s) 65, 216, 497, 1083, 1216, 1451, 1480, 2520, 3535
BmrFI CCNGG 6 cut(s) 29, 1525, 1907, 2022, 2522, 2738
BmrI ACTGGG 3 cut(s) 2556, 2983, 3004
BmsI GCATC 6 cut(s) 139, 870, 1025, 1068, 3121, 3184
BmtI GCTAGC 1 cut(s) 2688
BmuI ACTGGG 3 cut(s) 2556, 2983, 3004
BpiI GAAGAC 3 cut(s) 141, 1585, 1777
Bpu14I TTCGAA 3 cut(s) 132, 1752, 2353
BpuEI CTTGAG 4 cut(s) 1642, 2559, 2606, 3395
BpuMI CCSGG 3 cut(s) 29, 1525, 2522
BsaAI YACGTR 2 cut(s) 36, 3397
BsaBI GATNNNNATC 3 cut(s) 339, 525, 1387
BsaHI GRCGYC 2 cut(s) 496, 2874
BsaI GGTCTC 1 cut(s) 1520
BsaJI CCNNGG 5 cut(s) 328, 418, 699, 2736, 2737
BsaWI WCCGGW 2 cut(s) 1300, 1897
Bsc4I CCNNNNNNNGG 5 cut(s) 835, 2336, 2588, 2661, 3245
Bse118I RCCGGY 1 cut(s) 1475
Bse1I ACTGG 7 cut(s) 321, 1004, 2227, 2458, 2562, 2978, 3010
Bse3DI GCAATG 3 cut(s) 1470, 3087, 3503
Bse8I GATNNNNATC 3 cut(s) 339, 525, 1387
BseAI TCCGGA 1 cut(s) 1300
BseBI CCWGG 3 cut(s) 1907, 2022, 2738
BseDI CCNNGG 5 cut(s) 328, 418, 699, 2736, 2737
BseGI GGATG 8 cut(s) 169, 340, 526, 924, 1040, 1958, 2671, 3112
BseJI GATNNNNATC 3 cut(s) 339, 525, 1387
BseLI CCNNNNNNNGG 5 cut(s) 835, 2336, 2588, 2661, 3245
BseMI GCAATG 3 cut(s) 1470, 3087, 3503
BseMII CTCAG 4 cut(s) 31, 1245, 2505, 3423
BseNI ACTGG 7 cut(s) 321, 1004, 2227, 2458, 2562, 2978, 3010
BseRI GAGGAG 4 cut(s) 1074, 1077, 2147, 2150
BseX3I CGGCCG 1 cut(s) 7
BseXI GCAGC 6 cut(s) 423, 426, 790, 828, 1869, 2132
BseYI CCCAGC 1 cut(s) 3134
Bsh1236I CGCG 1 cut(s) 3313
Bsh1285I CGRYCG 2 cut(s) 10, 591
BshFI GGCC 7 cut(s) 9, 27, 744, 2280, 2849, 3145, 3249
BshNI GGYRCC 2 cut(s) 495, 1449
BsiEI CGRYCG 2 cut(s) 10, 591
BsiHKAI GWGCWC 3 cut(s) 2492, 2569, 3025
BsiSI CCGG 6 cut(s) 28, 1301, 1476, 1525, 1898, 2522
BslFI GGGAC 8 cut(s) 44, 1062, 1464, 1507, 2049, 2425, 2719, 3519
BslI CCNNNNNNNGG 5 cut(s) 835, 2336, 2588, 2661, 3245
BsmAI GTCTC 6 cut(s) 786, 825, 1234, 1520, 2410, 2725
BsmFI GGGAC 8 cut(s) 44, 1062, 1464, 1507, 2049, 2425, 2719, 3519
BsmI GAATGC 2 cut(s) 1333, 3409
BsnI GGCC 7 cut(s) 9, 27, 744, 2280, 2849, 3145, 3249
Bso31I GGTCTC 1 cut(s) 1520
Bsp119I TTCGAA 3 cut(s) 132, 1752, 2353
Bsp1286I GDGCHC 3 cut(s) 2492, 2569, 3025
Bsp13I TCCGGA 1 cut(s) 1300
Bsp1407I TGTACA 3 cut(s) 238, 1965, 2907
Bsp19I CCATGG 1 cut(s) 418
BspACI CCGC 7 cut(s) 10, 1218, 1860, 2306, 2626, 2675, 3531
BspANI GGCC 7 cut(s) 9, 27, 744, 2280, 2849, 3145, 3249
BspCNI CTCAG 4 cut(s) 30, 1244, 2506, 3424
BspEI TCCGGA 1 cut(s) 1300
BspFNI CGCG 1 cut(s) 3313
BspHI TCATGA 4 cut(s) 124, 885, 2964, 3519
BspLI GGNNCC 9 cut(s) 65, 216, 497, 1083, 1216, 1451, 1480, 2520, 3535
BspMI ACCTGC 1 cut(s) 371
BspOI GCTAGC 1 cut(s) 2688
BspPI GGATC 8 cut(s) 58, 71, 771, 783, 1889, 2057, 2573, 2625
BspQI GCTCTTC 1 cut(s) 3443
BspT104I TTCGAA 3 cut(s) 132, 1752, 2353
BspT107I GGYRCC 2 cut(s) 495, 1449
BspTNI GGTCTC 1 cut(s) 1520
BsrDI GCAATG 3 cut(s) 1470, 3087, 3503
BsrFI RCCGGY 1 cut(s) 1475
BsrGI TGTACA 3 cut(s) 238, 1965, 2907
BsrI ACTGG 7 cut(s) 321, 1004, 2227, 2458, 2562, 2978, 3010
BssAI RCCGGY 1 cut(s) 1475
BssECI CCNNGG 5 cut(s) 328, 418, 699, 2736, 2737
BssNI GRCGYC 2 cut(s) 496, 2874
BssT1I CCWWGG 2 cut(s) 418, 699
Bst2UI CCWGG 3 cut(s) 1907, 2022, 2738
Bst4CI ACNGT 6 cut(s) 892, 1068, 1117, 1176, 1783, 2733
Bst6I CTCTTC 4 cut(s) 1180, 2034, 2261, 3443
BstACI GRCGYC 2 cut(s) 496, 2874
BstAPI GCANNNNNTGC 1 cut(s) 1916
BstAUI TGTACA 3 cut(s) 238, 1965, 2907
BstBAI YACGTR 2 cut(s) 36, 3397
BstBI TTCGAA 3 cut(s) 132, 1752, 2353
BstC8I GCNNGC 7 cut(s) 51, 990, 1080, 2306, 2509, 2686, 3052
BstDEI CTNAG 7 cut(s) 17, 1055, 1231, 1864, 2514, 3432, 3564
BstDSI CCRYGG 2 cut(s) 328, 418
BstF5I GGATG 8 cut(s) 169, 340, 526, 924, 1040, 1958, 2671, 3112
BstFNI CGCG 1 cut(s) 3313
BstH2I RGCGCY 1 cut(s) 499
BstHHI GCGC 3 cut(s) 498, 1555, 3313
BstMAI GTCTC 6 cut(s) 786, 825, 1234, 1520, 2410, 2725
BstMCI CGRYCG 2 cut(s) 10, 591
BstNI CCWGG 3 cut(s) 1907, 2022, 2738
BstNSI RCATGY 2 cut(s) 958, 1445
BstSCI CCNGG 6 cut(s) 27, 1523, 1905, 2020, 2520, 2736
BstSFI CTRYAG 2 cut(s) 3061, 3100
BstUI CGCG 1 cut(s) 3313
BstV1I GCAGC 6 cut(s) 423, 426, 790, 828, 1869, 2132
BstV2I GAAGAC 3 cut(s) 141, 1585, 1777
BstX2I RGATCY 4 cut(s) 63, 2062, 2578, 2617
BstXI CCANNNNNNTGG 2 cut(s) 335, 425
BstYI RGATCY 4 cut(s) 63, 2062, 2578, 2617
BstZI CGGCCG 1 cut(s) 7
BsuI GTATCC 2 cut(s) 1623, 2888
BsuRI GGCC 7 cut(s) 9, 27, 744, 2280, 2849, 3145, 3249
BtgI CCRYGG 2 cut(s) 328, 418
BtgZI GCGATG 1 cut(s) 2355
BtsCI GGATG 8 cut(s) 169, 340, 526, 924, 1040, 1958, 2671, 3112
BtsI GCAGTG 2 cut(s) 1812, 3429
BtsIMutI CAGTG 6 cut(s) 351, 997, 1812, 2220, 2569, 3429
BveI ACCTGC 1 cut(s) 371
Cac8I GCNNGC 7 cut(s) 51, 990, 1080, 2306, 2509, 2686, 3052
CaiI CAGNNNCTG 2 cut(s) 2813, 3245
CciI TCATGA 4 cut(s) 124, 885, 2964, 3519
CfoI GCGC 3 cut(s) 498, 1555, 3313
Cfr10I RCCGGY 1 cut(s) 1475
Cfr13I GGNCC 8 cut(s) 204, 214, 742, 1467, 1478, 2518, 3248, 3533
CseI GACGC 1 cut(s) 2882
DdeI CTNAG 7 cut(s) 17, 1055, 1231, 1864, 2514, 3432, 3564
DinI GGCGCC 1 cut(s) 497
DrdI GACNNNNNNGTC 1 cut(s) 2753
DseDI GACNNNNNNGTC 1 cut(s) 2753
EaeI YGGCCR 1 cut(s) 7
EagI CGGCCG 1 cut(s) 7
Eam1104I CTCTTC 4 cut(s) 1180, 2034, 2261, 3443
EarI CTCTTC 4 cut(s) 1180, 2034, 2261, 3443
Ecl136II GAGCTC 1 cut(s) 3023
EclXI CGGCCG 1 cut(s) 7
Eco130I CCWWGG 2 cut(s) 418, 699
Eco147I AGGCCT 2 cut(s) 2280, 3145
Eco24I GRGCYC 1 cut(s) 3025
Eco31I GGTCTC 1 cut(s) 1520
Eco32I GATATC 1 cut(s) 538
Eco47I GGWCC 6 cut(s) 204, 214, 1467, 1478, 2518, 3533
Eco52I CGGCCG 1 cut(s) 7
Eco53kI GAGCTC 1 cut(s) 3023
Eco57I CTGAAG 2 cut(s) 1771, 2015
Eco72I CACGTG 1 cut(s) 36
EcoICRI GAGCTC 1 cut(s) 3023
EcoO109I RGGNCCY 2 cut(s) 214, 2518
EcoRI GAATTC 2 cut(s) 1680, 2369
EcoRII CCWGG 3 cut(s) 1905, 2020, 2736
EcoRV GATATC 1 cut(s) 538
EcoT14I CCWWGG 2 cut(s) 418, 699
EcoT22I ATGCAT 2 cut(s) 471, 1102
EcoT38I GRGCYC 1 cut(s) 3025
EgeI GGCGCC 1 cut(s) 497
EheI GGCGCC 1 cut(s) 497
ErhI CCWWGG 2 cut(s) 418, 699
FalI AAGNNNNNCTT 4 cut(s) 1011, 1043, 2375, 2407
FaqI GGGAC 8 cut(s) 44, 1062, 1464, 1507, 2049, 2425, 2719, 3519
FauI CCCGC 1 cut(s) 2313
FauNDI CATATG 1 cut(s) 465
FbaI TGATCA 1 cut(s) 178
Fnu4HI GCNGC 9 cut(s) 10, 412, 415, 804, 842, 1218, 1858, 1861, 2121
FokI GGATG 8 cut(s) 176, 327, 533, 931, 1047, 1945, 2658, 3119
FriOI GRGCYC 1 cut(s) 3025
Fsp4HI GCNGC 9 cut(s) 10, 412, 415, 804, 842, 1218, 1858, 1861, 2121
FspBI CTAG 9 cut(s) 305, 650, 1140, 1737, 2273, 2366, 2685, 2705, 3282
GlaI GCGC 3 cut(s) 497, 1554, 3312
GluI GCNGC 9 cut(s) 10, 412, 415, 804, 842, 1218, 1858, 1861, 2121
GsaI CCCAGC 1 cut(s) 3138
HaeII RGCGCY 1 cut(s) 499
HaeIII GGCC 7 cut(s) 9, 27, 744, 2280, 2849, 3145, 3249
HapII CCGG 6 cut(s) 28, 1301, 1476, 1525, 1898, 2522
HgaI GACGC 1 cut(s) 2882
HhaI GCGC 3 cut(s) 498, 1555, 3313
Hin1I GRCGYC 2 cut(s) 496, 2874
Hin6I GCGC 3 cut(s) 496, 1553, 3311
HinP1I GCGC 3 cut(s) 496, 1553, 3311
HincII GTYRAC 2 cut(s) 555, 2944
HindII GTYRAC 2 cut(s) 555, 2944
HindIII AAGCTT 1 cut(s) 296
HinfI GANTC 7 cut(s) 15, 112, 281, 1868, 2810, 2981, 3242
HpaI GTTAAC 1 cut(s) 2944
HpaII CCGG 6 cut(s) 28, 1301, 1476, 1525, 1898, 2522
HphI GGTGA 5 cut(s) 1258, 1267, 1897, 2251, 3420
Hpy166II GTNNAC 9 cut(s) 555, 583, 599, 1128, 1258, 1888, 1903, 2944, 3561
Hpy8I GTNNAC 9 cut(s) 555, 583, 599, 1128, 1258, 1888, 1903, 2944, 3561
HpyCH4III ACNGT 6 cut(s) 892, 1068, 1117, 1176, 1783, 2733
HpyCH4IV ACGT 5 cut(s) 35, 1532, 2881, 3273, 3396
HpyF3I CTNAG 7 cut(s) 17, 1055, 1231, 1864, 2514, 3432, 3564
HpySE526I ACGT 5 cut(s) 35, 1532, 2881, 3273, 3396
Hsp92I GRCGYC 2 cut(s) 496, 2874
HspAI GCGC 3 cut(s) 496, 1553, 3311
KasI GGCGCC 1 cut(s) 495
Kpn2I TCCGGA 1 cut(s) 1300
Ksp22I TGATCA 1 cut(s) 178
KspAI GTTAAC 1 cut(s) 2944
LguI GCTCTTC 1 cut(s) 3443
LmnI GCTCC 7 cut(s) 377, 985, 1075, 1087, 1214, 3020, 3190
Lsp1109I GCAGC 6 cut(s) 423, 426, 790, 828, 1869, 2132
LweI GCATC 6 cut(s) 139, 870, 1025, 1068, 3121, 3184
MaeI CTAG 9 cut(s) 305, 650, 1140, 1737, 2273, 2366, 2685, 2705, 3282
MaeII ACGT 5 cut(s) 35, 1532, 2881, 3273, 3396
MaeIII GTNAC 6 cut(s) 75, 231, 1176, 2545, 2986, 3426
MfeI CAATTG 1 cut(s) 2709
MflI RGATCY 4 cut(s) 63, 2062, 2578, 2617
MhlI GDGCHC 3 cut(s) 2492, 2569, 3025
Mly113I GGCGCC 1 cut(s) 496
MlyI GAGTC 2 cut(s) 9, 2975
MmeI TCCRAC 9 cut(s) 272, 896, 1192, 1272, 1360, 2005, 2433, 3144, 3318
Mph1103I ATGCAT 2 cut(s) 471, 1102
MroI TCCGGA 1 cut(s) 1300
MroXI GAANNNNTTC 4 cut(s) 645, 1262, 2349, 3081
MseI TTAA 8 cut(s) 387, 723, 1688, 1707, 1941, 2210, 2789, 2943
MslI CAYNNNNRTG 7 cut(s) 333, 423, 468, 617, 1109, 2148, 3129
MspA1I CMGCKG 1 cut(s) 1860
MspI CCGG 6 cut(s) 28, 1301, 1476, 1525, 1898, 2522
MspR9I CCNGG 6 cut(s) 29, 1525, 1907, 2022, 2522, 2738
MunI CAATTG 1 cut(s) 2709
Mva1269I GAATGC 2 cut(s) 1333, 3409
MvaI CCWGG 3 cut(s) 1907, 2022, 2738
MvnI CGCG 1 cut(s) 3313
NarI GGCGCC 1 cut(s) 496
NciI CCSGG 3 cut(s) 29, 1525, 2522
NcoI CCATGG 1 cut(s) 418
NdeI CATATG 1 cut(s) 465
NheI GCTAGC 1 cut(s) 2684
NlaIV GGNNCC 9 cut(s) 65, 216, 497, 1083, 1216, 1451, 1480, 2520, 3535
NmeAIII GCCGAG 1 cut(s) 2471
NmuCI GTSAC 3 cut(s) 2545, 2986, 3426
NsiI ATGCAT 2 cut(s) 471, 1102
NspI RCATGY 2 cut(s) 958, 1445
NspV TTCGAA 3 cut(s) 132, 1752, 2353
PagI TCATGA 4 cut(s) 124, 885, 2964, 3519
PaqCI CACCTGC 1 cut(s) 371
PasI CCCWGGG 1 cut(s) 2737
PceI AGGCCT 2 cut(s) 2280, 3145
PciI ACATGT 1 cut(s) 954
PciSI GCTCTTC 1 cut(s) 3443
PcsI WCGNNNNNNNCGW 1 cut(s) 1749
PctI GAATGC 2 cut(s) 1333, 3409
PdmI GAANNNNTTC 4 cut(s) 645, 1262, 2349, 3081
PfeI GAWTC 5 cut(s) 112, 281, 1868, 2810, 3242
PflFI GACNNNGTC 2 cut(s) 18, 2984
PflMI CCANNNNNTGG 2 cut(s) 2336, 3245
PkrI GCNGC 9 cut(s) 11, 413, 416, 805, 843, 1219, 1859, 1862, 2122
PleI GAGTC 2 cut(s) 9, 2975
PluTI GGCGCC 1 cut(s) 499
PmaCI CACGTG 1 cut(s) 36
PmlI CACGTG 1 cut(s) 36
PpsI GAGTC 2 cut(s) 9, 2975
Ppu21I YACGTR 2 cut(s) 36, 3397
PpuMI RGGWCCY 2 cut(s) 214, 2518
PscI ACATGT 1 cut(s) 954
PshBI ATTAAT 2 cut(s) 2210, 2789
PsiI TTATAA 3 cut(s) 1460, 2148, 2463
Psp124BI GAGCTC 1 cut(s) 3025
Psp5II RGGWCCY 2 cut(s) 214, 2518
Psp6I CCWGG 3 cut(s) 1905, 2020, 2736
PspCI CACGTG 1 cut(s) 36
PspFI CCCAGC 1 cut(s) 3134
PspGI CCWGG 3 cut(s) 1905, 2020, 2736
PspN4I GGNNCC 9 cut(s) 65, 216, 497, 1083, 1216, 1451, 1480, 2520, 3535
PspPI GGNCC 8 cut(s) 204, 214, 742, 1467, 1478, 2518, 3248, 3533
PspPPI RGGWCCY 2 cut(s) 214, 2518
PstNI CAGNNNCTG 2 cut(s) 2813, 3245
PsuI RGATCY 4 cut(s) 63, 2062, 2578, 2617
PsyI GACNNNGTC 2 cut(s) 18, 2984
RseI CAYNNNNRTG 7 cut(s) 333, 423, 468, 617, 1109, 2148, 3129
SacI GAGCTC 1 cut(s) 3025
SapI GCTCTTC 1 cut(s) 3443
SaqAI TTAA 8 cut(s) 387, 723, 1688, 1707, 1941, 2210, 2789, 2943
SatI GCNGC 9 cut(s) 10, 412, 415, 804, 842, 1218, 1858, 1861, 2121
Sau96I GGNCC 8 cut(s) 204, 214, 742, 1467, 1478, 2518, 3248, 3533
SchI GAGTC 2 cut(s) 9, 2975
ScrFI CCNGG 6 cut(s) 29, 1525, 1907, 2022, 2522, 2738
SduI GDGCHC 3 cut(s) 2492, 2569, 3025
SfaNI GCATC 6 cut(s) 139, 870, 1025, 1068, 3121, 3184
SfcI CTRYAG 2 cut(s) 3061, 3100
SfoI GGCGCC 1 cut(s) 497
SfuI TTCGAA 3 cut(s) 132, 1752, 2353
SinI GGWCC 6 cut(s) 204, 214, 1467, 1478, 2518, 3533
SmiMI CAYNNNNRTG 7 cut(s) 333, 423, 468, 617, 1109, 2148, 3129
SmlI CTYRAG 4 cut(s) 1657, 2574, 2621, 3410
SmoI CTYRAG 4 cut(s) 1657, 2574, 2621, 3410
SseBI AGGCCT 2 cut(s) 2280, 3145
SsiI CCGC 7 cut(s) 10, 1218, 1860, 2306, 2626, 2675, 3531
SspDI GGCGCC 1 cut(s) 495
SspI AATATT 2 cut(s) 1692, 2890
SspMI CTAG 9 cut(s) 305, 650, 1140, 1737, 2273, 2366, 2685, 2705, 3282
SstI GAGCTC 1 cut(s) 3025
StuI AGGCCT 2 cut(s) 2280, 3145
StyD4I CCNGG 6 cut(s) 27, 1523, 1905, 2020, 2520, 2736
StyI CCWWGG 2 cut(s) 418, 699
TaaI ACNGT 6 cut(s) 892, 1068, 1117, 1176, 1783, 2733
TaiI ACGT 5 cut(s) 38, 1535, 2884, 3276, 3399
TaqI TCGA 6 cut(s) 22, 132, 1484, 1752, 2291, 2353
TaqII GACCGA 1 cut(s) 577
TatI WGTACW 5 cut(s) 238, 1021, 1615, 1965, 2907
TauI GCSGC 3 cut(s) 12, 1220, 1863
TfiI GAWTC 5 cut(s) 112, 281, 1868, 2810, 3242
Tru1I TTAA 8 cut(s) 387, 723, 1688, 1707, 1941, 2210, 2789, 2943
Tru9I TTAA 8 cut(s) 387, 723, 1688, 1707, 1941, 2210, 2789, 2943
TscAI CASTG 6 cut(s) 358, 1004, 1812, 2227, 2569, 3429
TseFI GTSAC 3 cut(s) 2545, 2986, 3426
TseI GCWGC 6 cut(s) 411, 414, 803, 841, 1857, 2120
Tsp45I GTSAC 3 cut(s) 2545, 2986, 3426
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 6 cut(s) 358, 1004, 1812, 2227, 2569, 3429
Tth111I GACNNNGTC 2 cut(s) 18, 2984
Van91I CCANNNNNTGG 2 cut(s) 2336, 3245
VpaK11BI GGWCC 6 cut(s) 204, 214, 1467, 1478, 2518, 3533
VspI ATTAAT 2 cut(s) 2210, 2789
XceI RCATGY 2 cut(s) 958, 1445
XcmI CCANNNNNNNNNTGG 2 cut(s) 2450, 3131
XmnI GAANNNNTTC 4 cut(s) 645, 1262, 2349, 3081
XspI CTAG 9 cut(s) 305, 650, 1140, 1737, 2273, 2366, 2685, 2705, 3282
Zsp2I ATGCAT 2 cut(s) 471, 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.