Rroxscaffold_4G00294050

Senescence-associated carboxylesterase 101-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000388
Physical Location & Seq
Reverse (-)
14243027 .. 14261504
18478 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00294050.1

Sequence Viewer

Length: 2124 bp
ATGAAGGAGTTATGGCCGGAAGAAGGTGAAGAGGTCGGAGAGGATGACCGACTCGGGGAGAGAGAAGAGAGACAGCCCGCTCCCACACTCTCATGGGTAGGGGGTTTACTTACGGATGTCAAAATTCCGCAACGTTTGTCAGCGCATGGTGCCTCTCCCCTTAGGGAAGTTTGTTTGTGCAAAGCGTTGACCGCTACGTTGGGCGCCTTATTCATGGCAGATCGGCCTAATTTTTTTACACAATACCTATCAATGCTCCCACACTCTCATGGGGAGGGGTCTATCACCTGCTGTCAAAATACGGGGATGTTTGTCCGCAAAAGGTGCCGCTTCCCAAACGGAAGTTTGCTTATACAAAGTGTTGACCGCTCCATTTGGTTTAGCAGCGGCTTAGAATTGGGGAATTTTGTGGTGAACTCTGATCTGGTACACCAGGCATGGCGTGCGATTGAGCAACAAAGACAGAATAATCCAAATGCAGGGCCATCCTTGTACAAGAAAATCCAACCAGAAAATCCAATCGTCATAGCTTTTGTCACTCCACTTGGCTCTCTTCAGGGACAAGAAGGCTTGGTTTCATCAAGAGATTTTGCTCACTTAGAATTTTTGTGCAACAAAAGCAATCCAGTTTTCTGCATCAATGAAGCAGCAATCAAACTATTTCAGTCGCATTATAGTGACCTCCTCGTTCTGAAAAATAAGCTGGTAAGCAATTGCGATAGCGAATCCCCTTCATTAATAATCATCACCGGACAATCTGTGGGAGGTATTGTGGCTACACTCTTCACCTTATGGTTGCTAGAAGGCCTCGACTTGTCAAAAAGCAAACGCCCCCTTTGCATTACTTTCGGTTCTCCCCTTGTTGGCGATGAACACCTTCAAAAATGGGTGCTAAAATTCTCAACTTGGAAGTCTTGCTTCTTGCATATAGTCTCCGATCAAGATCATACACCGAAAATCTTTATGTCCCAAAACACAACTGTTGCTTATAAGCCGTTTGGAACATTCTTATTATGCTCGGCTTCGGGTTGTGCTTGCTCGGATAACCCGGAGTTCATTTTGCAACAATTTGTGACAACCAATTCACACAGTGCTCAAACTCAAGATCCTAATTTGGGGTTCCCTTATGGACAAATTTTGGAGGATCTCAAGCGCAAGGCATTATGTAATGTTTTCGAGTCCATTGGAGGGGAAAGAGGTCCACTTCAAACTAGCATAATCACACAACTCCTAGCAATTGGAGTAGTCCCACAGTCACAGAAACAAATTAGTGCAGAGAACAACCATCTGATTGAAATGATGAAAGAACATGAAACGAGATTAGCAATAATTGAGATGAAGAAATTTTTGAACTTCGACATGAAATTGAATGAAATGAAAGTGCACATGGCCTTCTTAGAGCGGTACAAGGAGGAAGGCAGACGCAGGAAAACAGGATACTACGACACGTACAAAAATCAGTGGGACCAGAATGACAAGAAAAATGAAGAGCATAAGAAGACGCTTTTGAATTACTGGGAGAACTCTGTCAGTGAAGTAGAGAACATGCCTCCAATAGAAAGAGCTCAGTTTCGGATTCGTTGGCTTTTTGCGGGCACAACCTATAGAAGGATGGTTGAACCACTTCACATTGCAGAATACTACAAGGACGGCGGGAAAAACTACCAAACTGAAGAGGGAAAAAGGCCTAAACATTTCATTCTGTTGGAGCAATGGGTGAAGACAGTACAGGGACCTAAAGATATCCCGAGCAGAAGACGGACCGTAGATTCTATTTTGAATAAGGATTCTTGTTTTTGGGCACATGTTGAGGAAGCTCTCATCTCGTGCAATACGCTGAAGGATGAAGAATCAAGTAGTACAGAGAGAGAATCTTCGCTGCAAAAATTGAAAGAATTTGAGGGATATGTGCTGGATTCCCTCAAGAATTGTGCAGTAAACCCTGAGATTTTCTTGGAGCGGAGCAGTTTCATGCAATGGTGGAATAAATATCAGGTGGGATTCGGGTCGACCGTCGAGGACTTTGATGGGCATTCGAAACGGGTTTTGAGCCGATGTTTTAAGCCAACAAGACCCTTTCGCATTGTCACATGGGGTGAGGATTTCCCTGGTAAATTTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

707

Amino Acids

80.78

Weight (kDa)

6.82

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 237 - 322 6.5e-13 Lipase (class 3)
EDS1_EP PF18117 466 - 666 9.1e-61 Enhanced disease susceptibility 1 protein EP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000388)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14930 AT5G14930 AT5G14930
fragaria_vesca FvH4_2g18461 FvH4_6g50160 FvH4_6g50160 FvH4_6g50160 FvH4_6g50162
malus_domestica MD01G1017800.v1.1 MD09G1038500.v1.1 MD09G1038700.v1.1 MD09G1039000.v1.1 MD09G1039700.v1.1 MD09G1039800.v1.1 MD17G1039600.v1.1 MD17G1039700.v1.1 MD17G1039800.v1.1 MD17G1039900.v1.1 MD17G1040000.v1.1
prunus_persica Prupe.2G019500_v2.0.a1 Prupe.3G279000_v2.0.a1 Prupe.3G279000_v2.0.a1 Prupe.3G279000_v2.0.a1 Prupe.3G279300_v2.0.a1
pyrus_communis pycom111g03070 pycom17g03570 pycom17g03580
rosa_chinensis RchiOBHm_Chr2g0170411 RchiOBHm_Chr2g0170431 RchiOBHm_Chr2g0170561 RchiOBHm_Chr6g0283951 RchiOBHm_Chr6g0283961 RchiOBHm_Chr6g0283981 RchiOBHm_Chr7g0219521 RchiOBHm_Chr7g0219541
rosa_laevigata RLG00000002285 RLG00000012782 RLG00000012784 RLG00000021954 RLG00000021955 RLG00000021956
rosa_multiflora Rmu_co8245087.1_g000001 Rmu_co8259259.1_g000001 Rmu_sc0003047.1_g000001 Rmu_sc0008697.1_g000004 Rmu_sc0009395.1_g000014 Rmu_sc0009395.1_g000016 Rmu_sc0009395.1_g000019 Rmu_sc0009395.1_g000026 Rmu_sc0009395.1_g000028 Rmu_sc0009395.1_g000040 Rmu_sc0021809.1_g000001 Rmu_ssc0000442.1_g000026 Rmu_ssc0000442.1_g000030
rosa_roxburghii Rroxscaffold_2G00081280 Rroxscaffold_2G00081290 Rroxscaffold_2G00081330 Rroxscaffold_3G00239990 Rroxscaffold_4G00294050 Rroxscaffold_4G00294070 Rroxscaffold_7G00185030
rosa_rugosa Rorug02G0548700 Rorug02G0548900 Rorug06G0158800 Rorug06G0158900 Rorug07G0186000
rosa_samantha Rh2AG620300 Rh2AG620500 Rh2AG620600 Rh2AG620700 Rh2BG631200 Rh2BG631400 Rh2BG631600 Rh2BG631700 Rh2CG601100 Rh2CG601200 Rh2CG601300 Rh2DG643900 Rh2DG644200 Rh2DG644400 Rh6AG272000 Rh6AG272300 Rh6BG272900 Rh6BG273000 Rh6CG274100 Rh6CG274200 Rh6DG267100 Rh6DG267200 Rh6DG267300 Rh7AG329500 Rh7AG329900 Rh7BG319400 Rh7BG319600 Rh7CG346800 Rh7CG347200 Rh7DG325400
rosa_wichuraiana Rw2G051410 Rw2G051430 Rw2G051440 Rw2G051450 Rw6G023440 Rw7G027780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 990
AarI CACCTGC 1 cut(s) 296
Acc36I ACCTGC 1 cut(s) 296
AccB1I GGYRCC 3 cut(s) 149, 203, 324
AccBSI CCGCTC 4 cut(s) 80, 369, 1402, 1960
AccI GTMKAC 1 cut(s) 2009
AclI AACGTT 1 cut(s) 133
AclWI GGATC 2 cut(s) 1100, 1152
AcoI YGGCCR 1 cut(s) 14
AcsI RAATTY 8 cut(s) 123, 403, 602, 896, 1134, 1343, 1895, 2114
AcuI CTGAAG 3 cut(s) 539, 1692, 1859
AcyI GRCGYC 1 cut(s) 204
AdeI CACNNNGTG 1 cut(s) 1091
AfaI GTAC 6 cut(s) 429, 494, 1406, 1451, 1728, 1861
AfiI CCNNNNNNNGG 7 cut(s) 23, 55, 479, 863, 1115, 1188, 1608
AflIII ACRYGT 2 cut(s) 1446, 1804
AgsI TTSAA 9 cut(s) 881, 1208, 1295, 1351, 1369, 1510, 1619, 1780, 1891
AjnI CCWGG 2 cut(s) 432, 2107
AluBI AGCT 4 cut(s) 530, 703, 1565, 1817
AluI AGCT 4 cut(s) 530, 703, 1565, 1817
Alw21I GWGCWC 3 cut(s) 1096, 1386, 1567
Alw26I GTCTC 2 cut(s) 64, 937
Alw44I GTGCAC 1 cut(s) 1382
AlwI GGATC 2 cut(s) 1100, 1152
Ama87I CYCGRG 2 cut(s) 53, 1747
AoxI GGCC 6 cut(s) 14, 224, 482, 805, 1389, 1685
ApaLI GTGCAC 1 cut(s) 1382
ApeKI GCWGC 3 cut(s) 384, 647, 1879
ApoI RAATTY 8 cut(s) 123, 403, 602, 896, 1134, 1343, 1895, 2114
AseI ATTAAT 1 cut(s) 737
Asp700I GAANNNNTTC 2 cut(s) 876, 1623
AspLEI GCGC 3 cut(s) 145, 206, 1155
AspS9I GGNCC 5 cut(s) 482, 1199, 1465, 1733, 1761
AsuC2I CCSGG 1 cut(s) 1049
AsuHPI GGTGA 7 cut(s) 38, 277, 424, 739, 778, 1729, 2108
AsuII TTCGAA 1 cut(s) 2036
AvaI CYCGRG 2 cut(s) 53, 1747
AvaII GGWCC 4 cut(s) 1199, 1465, 1733, 1761
AxyI CCTNAGG 1 cut(s) 161
BaeGI GKGCMC 3 cut(s) 1386, 1598, 1804
BanI GGYRCC 3 cut(s) 149, 203, 324
BanII GRGCYC 1 cut(s) 1567
BauI CACGAG 1 cut(s) 1825
BbsI GAAGAC 3 cut(s) 1505, 1727, 1762
Bbv12I GWGCWC 3 cut(s) 1096, 1386, 1567
BbvI GCAGC 3 cut(s) 396, 659, 1866
BccI CCATC 4 cut(s) 493, 1293, 1606, 2021
BceAI ACGGC 2 cut(s) 979, 1666
BcgI CGANNNNNNTGC 2 cut(s) 829, 863
BciT130I CCWGG 2 cut(s) 434, 2109
BciVI GTATCC 1 cut(s) 1430
BcnI CCSGG 1 cut(s) 1049
BcoDI GTCTC 2 cut(s) 64, 937
BfaI CTAG 3 cut(s) 800, 1212, 1232
BfmI CTRYAG 1 cut(s) 1603
BfoI RGCGCY 1 cut(s) 207
BfuAI ACCTGC 1 cut(s) 296
BfuI GTATCC 1 cut(s) 1430
BisI GCNGC 5 cut(s) 328, 385, 388, 648, 1880
BlsI GCNGC 5 cut(s) 329, 386, 389, 649, 1881
Bme1390I CCNGG 3 cut(s) 434, 1049, 2109
Bme18I GGWCC 4 cut(s) 1199, 1465, 1733, 1761
BmeT110I CYCGRG 2 cut(s) 53, 1747
BmgT120I GGNCC 5 cut(s) 482, 1199, 1465, 1733, 1761
BmiI GGNNCC 6 cut(s) 151, 205, 326, 1121, 1466, 1734
BmrFI CCNGG 3 cut(s) 434, 1049, 2109
BmrI ACTGGG 1 cut(s) 1525
BmsI GCATC 1 cut(s) 645
BmuI ACTGGG 1 cut(s) 1525
BpiI GAAGAC 3 cut(s) 1505, 1727, 1762
Bpu14I TTCGAA 1 cut(s) 2036
BpuEI CTTGAG 3 cut(s) 1086, 1133, 1907
BpuMI CCSGG 1 cut(s) 1049
BsaAI YACGTR 1 cut(s) 1449
BsaBI GATNNNNATC 1 cut(s) 942
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 2107
BsaWI WCCGGW 1 cut(s) 749
BsaXI ACNNNNNCTCC 2 cut(s) 756, 786
Bsc4I CCNNNNNNNGG 7 cut(s) 23, 55, 479, 863, 1115, 1188, 1608
Bse1I ACTGG 2 cut(s) 626, 1520
Bse21I CCTNAGG 1 cut(s) 161
Bse3DI GCAATG 3 cut(s) 1629, 1718, 1982
Bse8I GATNNNNATC 1 cut(s) 942
BseBI CCWGG 2 cut(s) 434, 2109
BseDI CCNNGG 1 cut(s) 2107
BseGI GGATG 6 cut(s) 49, 121, 312, 485, 1617, 1849
BseJI GATNNNNATC 1 cut(s) 942
BseLI CCNNNNNNNGG 7 cut(s) 23, 55, 479, 863, 1115, 1188, 1608
BseMI GCAATG 3 cut(s) 1629, 1718, 1982
BseMII CTCAG 2 cut(s) 1580, 1935
BseNI ACTGG 2 cut(s) 626, 1520
BseRI GAGGAG 1 cut(s) 674
BseSI GKGCMC 3 cut(s) 1386, 1598, 1804
BseXI GCAGC 3 cut(s) 396, 659, 1866
BsgI GTGCAG 2 cut(s) 1293, 1953
Bsh1285I CGRYCG 1 cut(s) 2013
BshFI GGCC 6 cut(s) 16, 226, 484, 807, 1391, 1687
BshNI GGYRCC 3 cut(s) 149, 203, 324
BsiEI CGRYCG 1 cut(s) 2013
BsiHKAI GWGCWC 3 cut(s) 1096, 1386, 1567
BsiHKCI CYCGRG 2 cut(s) 53, 1747
BsiSI CCGG 3 cut(s) 17, 750, 1049
BslFI GGGAC 5 cut(s) 573, 952, 1232, 1478, 1746
BslI CCNNNNNNNGG 7 cut(s) 23, 55, 479, 863, 1115, 1188, 1608
BsmAI GTCTC 2 cut(s) 64, 937
BsmFI GGGAC 5 cut(s) 573, 952, 1232, 1478, 1746
BsmI GAATGC 1 cut(s) 2032
BsnI GGCC 6 cut(s) 16, 226, 484, 807, 1391, 1687
BsoBI CYCGRG 2 cut(s) 53, 1747
Bsp119I TTCGAA 1 cut(s) 2036
Bsp1286I GDGCHC 5 cut(s) 1096, 1386, 1567, 1598, 1804
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 6 cut(s) 220, 421, 937, 943, 1105, 1144
BspANI GGCC 6 cut(s) 16, 226, 484, 807, 1391, 1687
BspCNI CTCAG 2 cut(s) 1579, 1936
BspLI GGNNCC 6 cut(s) 151, 205, 326, 1121, 1466, 1734
BspMI ACCTGC 1 cut(s) 296
BspPI GGATC 2 cut(s) 1100, 1152
BspQI GCTCTTC 1 cut(s) 1482
BspT104I TTCGAA 1 cut(s) 2036
BspT107I GGYRCC 3 cut(s) 149, 203, 324
BsrBI CCGCTC 4 cut(s) 80, 369, 1402, 1960
BsrDI GCAATG 3 cut(s) 1629, 1718, 1982
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 2 cut(s) 626, 1520
BssECI CCNNGG 1 cut(s) 2107
BssMI GATC 6 cut(s) 220, 421, 937, 943, 1105, 1144
BssNI GRCGYC 1 cut(s) 204
BssSI CACGAG 1 cut(s) 1825
Bst2BI CACGAG 1 cut(s) 1825
Bst2UI CCWGG 2 cut(s) 434, 2109
Bst4CI ACNGT 6 cut(s) 982, 1091, 1254, 1726, 1765, 2014
Bst6I CTCTTC 6 cut(s) 24, 60, 558, 788, 1482, 1668
BstACI GRCGYC 1 cut(s) 204
BstAPI GCANNNNNTGC 2 cut(s) 324, 443
BstAUI TGTACA 1 cut(s) 492
BstBAI YACGTR 1 cut(s) 1449
BstBI TTCGAA 1 cut(s) 2036
BstC8I GCNNGC 4 cut(s) 78, 444, 1036, 1594
BstDEI CTNAG 6 cut(s) 161, 391, 598, 1396, 1566, 1944
BstENI CCTNNNNNAGG 1 cut(s) 1606
BstF5I GGATG 6 cut(s) 49, 121, 312, 485, 1617, 1849
BstH2I RGCGCY 1 cut(s) 207
BstHHI GCGC 3 cut(s) 145, 206, 1155
BstKTI GATC 6 cut(s) 223, 424, 940, 946, 1108, 1147
BstMAI GTCTC 2 cut(s) 64, 937
BstMBI GATC 6 cut(s) 220, 421, 937, 943, 1105, 1144
BstMCI CGRYCG 1 cut(s) 2013
BstMWI GCNNNNNNNGC 6 cut(s) 149, 191, 324, 443, 618, 837
BstNI CCWGG 2 cut(s) 434, 2109
BstNSI RCATGY 2 cut(s) 1549, 1808
BstSCI CCNGG 3 cut(s) 432, 1047, 2107
BstSFI CTRYAG 1 cut(s) 1603
BstSLI GKGCMC 3 cut(s) 1386, 1598, 1804
BstV1I GCAGC 3 cut(s) 396, 659, 1866
BstV2I GAAGAC 3 cut(s) 1505, 1727, 1762
BstX2I RGATCY 2 cut(s) 1105, 1144
BstYI RGATCY 2 cut(s) 1105, 1144
Bsu36I CCTNAGG 1 cut(s) 161
BsuI GTATCC 1 cut(s) 1430
BsuRI GGCC 6 cut(s) 16, 226, 484, 807, 1391, 1687
BtgZI GCGATG 1 cut(s) 882
BtsCI GGATG 6 cut(s) 49, 121, 312, 485, 1617, 1849
BtsIMutI CAGTG 3 cut(s) 1096, 1466, 1537
BveI ACCTGC 1 cut(s) 296
Cac8I GCNNGC 4 cut(s) 78, 444, 1036, 1594
CfoI GCGC 3 cut(s) 145, 206, 1155
Cfr13I GGNCC 5 cut(s) 482, 1199, 1465, 1733, 1761
CpoI CGGWCCG 1 cut(s) 1761
CseI GACGC 2 cut(s) 1431, 1510
Csp6I GTAC 6 cut(s) 428, 493, 1405, 1450, 1727, 1860
CspI CGGWCCG 1 cut(s) 1761
CviQI GTAC 6 cut(s) 428, 493, 1405, 1450, 1727, 1860
DdeI CTNAG 6 cut(s) 161, 391, 598, 1396, 1566, 1944
DinI GGCGCC 1 cut(s) 205
DpnI GATC 6 cut(s) 222, 423, 939, 945, 1107, 1146
DpnII GATC 6 cut(s) 220, 421, 937, 943, 1105, 1144
DraIII CACNNNGTG 1 cut(s) 1091
EaeI YGGCCR 1 cut(s) 14
Eam1104I CTCTTC 6 cut(s) 24, 60, 558, 788, 1482, 1668
EarI CTCTTC 6 cut(s) 24, 60, 558, 788, 1482, 1668
Ecl136II GAGCTC 1 cut(s) 1565
Eco147I AGGCCT 2 cut(s) 807, 1687
Eco24I GRGCYC 1 cut(s) 1567
Eco32I GATATC 1 cut(s) 1744
Eco47I GGWCC 4 cut(s) 1199, 1465, 1733, 1761
Eco53kI GAGCTC 1 cut(s) 1565
Eco57I CTGAAG 3 cut(s) 539, 1692, 1859
Eco81I CCTNAGG 1 cut(s) 161
Eco88I CYCGRG 2 cut(s) 53, 1747
EcoICRI GAGCTC 1 cut(s) 1565
EcoNI CCTNNNNNAGG 1 cut(s) 1606
EcoO109I RGGNCCY 1 cut(s) 1733
EcoRII CCWGG 2 cut(s) 432, 2107
EcoRV GATATC 1 cut(s) 1744
EcoT38I GRGCYC 1 cut(s) 1567
EgeI GGCGCC 1 cut(s) 205
EheI GGCGCC 1 cut(s) 205
FalI AAGNNNNNCTT 2 cut(s) 902, 934
FaqI GGGAC 5 cut(s) 573, 952, 1232, 1478, 1746
FauI CCCGC 3 cut(s) 85, 1585, 1646
FblI GTMKAC 1 cut(s) 2009
Fnu4HI GCNGC 5 cut(s) 328, 385, 388, 648, 1880
FokI GGATG 6 cut(s) 56, 128, 319, 472, 1624, 1856
FriOI GRGCYC 1 cut(s) 1567
Fsp4HI GCNGC 5 cut(s) 328, 385, 388, 648, 1880
FspBI CTAG 3 cut(s) 800, 1212, 1232
GlaI GCGC 3 cut(s) 144, 205, 1154
GluI GCNGC 5 cut(s) 328, 385, 388, 648, 1880
HaeII RGCGCY 1 cut(s) 207
HaeIII GGCC 6 cut(s) 16, 226, 484, 807, 1391, 1687
HapII CCGG 3 cut(s) 17, 750, 1049
HgaI GACGC 2 cut(s) 1431, 1510
HhaI GCGC 3 cut(s) 145, 206, 1155
Hin1I GRCGYC 1 cut(s) 204
Hin6I GCGC 3 cut(s) 143, 204, 1153
HinP1I GCGC 3 cut(s) 143, 204, 1153
HincII GTYRAC 3 cut(s) 189, 364, 2010
HindII GTYRAC 3 cut(s) 189, 364, 2010
HpaII CCGG 3 cut(s) 17, 750, 1049
HphI GGTGA 7 cut(s) 38, 277, 424, 739, 778, 1729, 2108
Hpy166II GTNNAC 9 cut(s) 107, 189, 364, 415, 430, 1202, 1384, 1939, 2010
Hpy188I TCNGA 7 cut(s) 38, 421, 693, 937, 1042, 1290, 1575
Hpy188III TCNNGA 5 cut(s) 582, 941, 1103, 1747, 1924
Hpy8I GTNNAC 9 cut(s) 107, 189, 364, 415, 430, 1202, 1384, 1939, 2010
Hpy99I CGWCG 1 cut(s) 2018
HpyAV CCTTC 9 cut(s) 17, 560, 741, 797, 887, 1402, 1409, 1602, 1834
HpyCH4III ACNGT 6 cut(s) 982, 1091, 1254, 1726, 1765, 2014
HpyCH4IV ACGT 3 cut(s) 133, 197, 1448
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 191, 324, 443, 618, 837
HpyF3I CTNAG 6 cut(s) 161, 391, 598, 1396, 1566, 1944
HpySE526I ACGT 3 cut(s) 133, 197, 1448
Hsp92I GRCGYC 1 cut(s) 204
HspAI GCGC 3 cut(s) 143, 204, 1153
KasI GGCGCC 1 cut(s) 203
Kzo9I GATC 6 cut(s) 220, 421, 937, 943, 1105, 1144
LguI GCTCTTC 1 cut(s) 1482
LmnI GCTCC 6 cut(s) 85, 261, 374, 1708, 1957, 1962
Lsp1109I GCAGC 3 cut(s) 396, 659, 1866
LweI GCATC 1 cut(s) 645
MaeI CTAG 3 cut(s) 800, 1212, 1232
MaeII ACGT 3 cut(s) 133, 197, 1448
MaeIII GTNAC 5 cut(s) 535, 677, 1072, 1254, 2086
MalI GATC 6 cut(s) 222, 423, 939, 945, 1107, 1146
MbiI CCGCTC 4 cut(s) 80, 369, 1402, 1960
MboI GATC 6 cut(s) 220, 421, 937, 943, 1105, 1144
MfeI CAATTG 2 cut(s) 712, 1236
MflI RGATCY 2 cut(s) 1105, 1144
MhlI GDGCHC 5 cut(s) 1096, 1386, 1567, 1598, 1804
Mly113I GGCGCC 1 cut(s) 204
MlyI GAGTC 2 cut(s) 45, 1187
MmeI TCCRAC 3 cut(s) 16, 529, 1686
MroXI GAANNNNTTC 2 cut(s) 876, 1623
MseI TTAA 2 cut(s) 737, 2061
MslI CAYNNNNRTG 3 cut(s) 91, 267, 675
MspA1I CMGCKG 1 cut(s) 387
MspI CCGG 3 cut(s) 17, 750, 1049
MspR9I CCNGG 3 cut(s) 434, 1049, 2109
MunI CAATTG 2 cut(s) 712, 1236
Mva1269I GAATGC 1 cut(s) 2032
MvaI CCWGG 2 cut(s) 434, 2109
MwoI GCNNNNNNNGC 6 cut(s) 149, 191, 324, 443, 618, 837
NarI GGCGCC 1 cut(s) 204
NciI CCSGG 1 cut(s) 1049
NdeII GATC 6 cut(s) 220, 421, 937, 943, 1105, 1144
NlaIV GGNNCC 6 cut(s) 151, 205, 326, 1121, 1466, 1734
NmeAIII GCCGAG 1 cut(s) 998
NmuCI GTSAC 5 cut(s) 535, 677, 1072, 1254, 2086
NspI RCATGY 2 cut(s) 1549, 1808
NspV TTCGAA 1 cut(s) 2036
PaqCI CACCTGC 1 cut(s) 296
PceI AGGCCT 2 cut(s) 807, 1687
PciI ACATGT 1 cut(s) 1804
PciSI GCTCTTC 1 cut(s) 1482
PcsI WCGNNNNNNNCGW 1 cut(s) 2010
PctI GAATGC 1 cut(s) 2032
PdmI GAANNNNTTC 2 cut(s) 876, 1623
PfeI GAWTC 8 cut(s) 725, 1576, 1769, 1787, 1850, 1871, 1916, 2001
PkrI GCNGC 5 cut(s) 329, 386, 389, 649, 1881
PleI GAGTC 2 cut(s) 45, 1186
PluTI GGCGCC 1 cut(s) 207
PpsI GAGTC 2 cut(s) 45, 1186
Ppu21I YACGTR 1 cut(s) 1449
PpuMI RGGWCCY 1 cut(s) 1733
PscI ACATGT 1 cut(s) 1804
PshBI ATTAAT 1 cut(s) 737
PsiI TTATAA 1 cut(s) 990
Psp124BI GAGCTC 1 cut(s) 1567
Psp1406I AACGTT 1 cut(s) 133
Psp5II RGGWCCY 1 cut(s) 1733
Psp6I CCWGG 2 cut(s) 432, 2107
PspGI CCWGG 2 cut(s) 432, 2107
PspN4I GGNNCC 6 cut(s) 151, 205, 326, 1121, 1466, 1734
PspPI GGNCC 5 cut(s) 482, 1199, 1465, 1733, 1761
PspPPI RGGWCCY 1 cut(s) 1733
PsuI RGATCY 2 cut(s) 1105, 1144
RsaI GTAC 6 cut(s) 429, 494, 1406, 1451, 1728, 1861
RsaNI GTAC 6 cut(s) 428, 493, 1405, 1450, 1727, 1860
RseI CAYNNNNRTG 3 cut(s) 91, 267, 675
Rsr2I CGGWCCG 1 cut(s) 1761
RsrII CGGWCCG 1 cut(s) 1761
SacI GAGCTC 1 cut(s) 1567
SalI GTCGAC 1 cut(s) 2008
SapI GCTCTTC 1 cut(s) 1482
SaqAI TTAA 2 cut(s) 737, 2061
SatI GCNGC 5 cut(s) 328, 385, 388, 648, 1880
Sau3AI GATC 6 cut(s) 220, 421, 937, 943, 1105, 1144
Sau96I GGNCC 5 cut(s) 482, 1199, 1465, 1733, 1761
SchI GAGTC 2 cut(s) 45, 1187
ScrFI CCNGG 3 cut(s) 434, 1049, 2109
SduI GDGCHC 5 cut(s) 1096, 1386, 1567, 1598, 1804
SfaNI GCATC 1 cut(s) 645
SfcI CTRYAG 1 cut(s) 1603
SfoI GGCGCC 1 cut(s) 205
SfuI TTCGAA 1 cut(s) 2036
SinI GGWCC 4 cut(s) 1199, 1465, 1733, 1761
SmiMI CAYNNNNRTG 3 cut(s) 91, 267, 675
SmlI CTYRAG 3 cut(s) 1101, 1148, 1922
SmoI CTYRAG 3 cut(s) 1101, 1148, 1922
SseBI AGGCCT 2 cut(s) 807, 1687
SspDI GGCGCC 1 cut(s) 203
SspMI CTAG 3 cut(s) 800, 1212, 1232
SstI GAGCTC 1 cut(s) 1567
StuI AGGCCT 2 cut(s) 807, 1687
StyD4I CCNGG 3 cut(s) 432, 1047, 2107
TaaI ACNGT 6 cut(s) 982, 1091, 1254, 1726, 1765, 2014
TaiI ACGT 3 cut(s) 136, 200, 1451
TaqI TCGA 6 cut(s) 810, 1176, 1356, 2009, 2016, 2036
TaqII GACCGA 1 cut(s) 63
TatI WGTACW 3 cut(s) 492, 1726, 1859
TauI GCSGC 2 cut(s) 330, 390
TfiI GAWTC 8 cut(s) 725, 1576, 1769, 1787, 1850, 1871, 1916, 2001
Tru1I TTAA 2 cut(s) 737, 2061
Tru9I TTAA 2 cut(s) 737, 2061
TscAI CASTG 3 cut(s) 1096, 1466, 1537
TseFI GTSAC 5 cut(s) 535, 677, 1072, 1254, 2086
TseI GCWGC 3 cut(s) 384, 647, 1879
Tsp45I GTSAC 5 cut(s) 535, 677, 1072, 1254, 2086
TspGWI ACGGA 3 cut(s) 128, 354, 1774
TspRI CASTG 3 cut(s) 1096, 1466, 1537
VneI GTGCAC 1 cut(s) 1382
VpaK11BI GGWCC 4 cut(s) 1199, 1465, 1733, 1761
VspI ATTAAT 1 cut(s) 737
XagI CCTNNNNNAGG 1 cut(s) 1606
XapI RAATTY 8 cut(s) 123, 403, 602, 896, 1134, 1343, 1895, 2114
XceI RCATGY 2 cut(s) 1549, 1808
XmiI GTMKAC 1 cut(s) 2009
XmnI GAANNNNTTC 2 cut(s) 876, 1623
XspI CTAG 3 cut(s) 800, 1212, 1232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.