pycom01g06090

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000713
Physical Location & Seq
Forward (+)
6182343 .. 6186462
4120 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g06090.3

Sequence Viewer

Length: 981 bp
ATGAACTTGCGTCCTTGGATCGATCCAGGAACTCCGATAACCTTGGAGGAGGACAGTGAATTTCGGCGATGGGACGAGCTCATACCTGATGCACTTGGGCTGATATTTAGGAATCTAACCCTTCAAGAGACACTCACTGTAATCCCAAGGGTGTGCAAGTCATGGGGAAGAGCAGTGTCTGGACCTTATTGCTGGCAGGAGATAGACATTGCGGAATGGAGCAAATTTCGTCCGCCAGAGACTGTTACGCGAATGCTGCAAATGCTGGTCATAAGAAGCTCAGGTTCACTCCGAAAGCTCTTCGTTGCTGGCCTTCCCAATGACCAGACTTTCTCCTTTATTGCTGATCATGCCAAATCTCTTCAGACACTGCAGCTGCCAAGAAGTGAAATAAGTGATTCCATAGTGGAACAGGTTGCTGCAAGGCTCTCTTCCATAACTTTCTTGGATGTAAGCTACTGCAAAACTATTGGAGGCCCTGCACTTGAGGCATTTGGGAAGCATTGTAGACATCTCAGAAAGTTGCAGAGATGCATGTACGGATGGGAGGTACTTGACAAGGTTTCTCAAGATGACGAGGCCCTTGCAATAGCGGCCACAATGCCAAAACTCAAGCACCTTGAGATACTCTTTCTGCATGTTAGTACAGAAGCTGTGCGTCAGATACTCTTGAACTGCCCTGAGCTCCAGATATTAGATGTGCGAGGGTGCTATAGTGTGAAACTTGATGAAAAGTTTGTCAAGAAATTCTCGGGGCTGAAGCTGGTGGGACCAGGTTCCGACAATGCGGGCTGGGATGATTGTTCAGATTACTCAGTTTCTTCTGGCTATTTAGCATGGGATGTAATGGATGATGATTTTGACGAAGAGATGTTAGATGAGGCTTGGGAAGACGACCTTAGTATGGAGGATGTAGAAATGAGGTTCTATGATGGTTTTTATCTCGATAATGGTGGGTTCGATTGGCCCCTATCTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

327

Amino Acids

37.15

Weight (kDa)

4.64

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 508
AccII CGCG 1 cut(s) 250
AciI CCGC 4 cut(s) 212, 233, 593, 788
AclWI GGATC 2 cut(s) 17, 26
AcoI YGGCCR 1 cut(s) 594
AcsI RAATTY 3 cut(s) 59, 224, 746
AcuI CTGAAG 2 cut(s) 347, 779
AfaI GTAC 3 cut(s) 539, 552, 646
AfiI CCNNNNNNNGG 1 cut(s) 904
AgsI TTSAA 2 cut(s) 125, 673
AjnI CCWGG 2 cut(s) 25, 772
AluBI AGCT 8 cut(s) 79, 279, 298, 376, 456, 653, 685, 763
AluI AGCT 8 cut(s) 79, 279, 298, 376, 456, 653, 685, 763
Alw21I GWGCWC 2 cut(s) 81, 687
Alw26I GTCTC 2 cut(s) 122, 233
AlwI GGATC 2 cut(s) 17, 26
AlwNI CAGNNNCTG 4 cut(s) 179, 242, 370, 653
Ama87I CYCGRG 1 cut(s) 751
AoxI GGCC 5 cut(s) 310, 475, 579, 594, 965
ApeKI GCWGC 4 cut(s) 256, 373, 376, 419
ApoI RAATTY 3 cut(s) 59, 224, 746
AspS9I GGNCC 5 cut(s) 182, 476, 580, 770, 966
AvaI CYCGRG 1 cut(s) 751
AvaII GGWCC 2 cut(s) 182, 770
BanII GRGCYC 2 cut(s) 81, 687
BbsI GAAGAC 1 cut(s) 897
Bbv12I GWGCWC 2 cut(s) 81, 687
BbvI GCAGC 4 cut(s) 243, 363, 385, 406
BccI CCATC 3 cut(s) 63, 537, 926
BcgI CGANNNNNNTGC 2 cut(s) 566, 600
BciT130I CCWGG 2 cut(s) 27, 774
BclI TGATCA 1 cut(s) 346
BcoDI GTCTC 2 cut(s) 122, 233
BfmI CTRYAG 2 cut(s) 371, 712
BisI GCNGC 5 cut(s) 257, 374, 377, 420, 594
BlsI GCNGC 5 cut(s) 258, 375, 378, 421, 595
Bme1390I CCNGG 2 cut(s) 27, 774
Bme18I GGWCC 2 cut(s) 182, 770
BmeT110I CYCGRG 1 cut(s) 751
BmgT120I GGNCC 5 cut(s) 182, 476, 580, 770, 966
BmiI GGNNCC 3 cut(s) 771, 778, 968
BmrFI CCNGG 2 cut(s) 27, 774
BmsI GCATC 2 cut(s) 79, 521
BpiI GAAGAC 1 cut(s) 897
BpmI CTGGAG 1 cut(s) 671
Bpu10I CCTNAGC 2 cut(s) 280, 681
BpuEI CTTGAG 4 cut(s) 506, 552, 596, 641
Bsa29I ATCGAT 1 cut(s) 21
BsaJI CCNNGG 3 cut(s) 14, 42, 146
Bsc4I CCNNNNNNNGG 1 cut(s) 904
Bse3DI GCAATG 1 cut(s) 207
BseBI CCWGG 2 cut(s) 27, 774
BseCI ATCGAT 1 cut(s) 21
BseDI CCNNGG 3 cut(s) 14, 42, 146
BseGI GGATG 6 cut(s) 454, 548, 802, 847, 856, 916
BseLI CCNNNNNNNGG 1 cut(s) 904
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 4 cut(s) 294, 529, 672, 828
BseRI GAGGAG 1 cut(s) 62
BseXI GCAGC 4 cut(s) 243, 363, 385, 406
BseYI CCCAGC 1 cut(s) 792
BsgI GTGCAG 1 cut(s) 465
Bsh1236I CGCG 1 cut(s) 250
BshFI GGCC 5 cut(s) 312, 477, 581, 596, 967
BshVI ATCGAT 1 cut(s) 21
BsiHKAI GWGCWC 2 cut(s) 81, 687
BsiHKCI CYCGRG 1 cut(s) 751
BslFI GGGAC 2 cut(s) 86, 783
BslI CCNNNNNNNGG 1 cut(s) 904
BsmAI GTCTC 2 cut(s) 122, 233
BsmFI GGGAC 2 cut(s) 86, 783
BsmI GAATGC 1 cut(s) 258
BsnI GGCC 5 cut(s) 312, 477, 581, 596, 967
BsoBI CYCGRG 1 cut(s) 751
Bsp1286I GDGCHC 2 cut(s) 81, 687
Bsp143I GATC 3 cut(s) 18, 22, 346
BspACI CCGC 4 cut(s) 212, 233, 593, 788
BspANI GGCC 5 cut(s) 312, 477, 581, 596, 967
BspCNI CTCAG 4 cut(s) 293, 528, 673, 827
BspDI ATCGAT 1 cut(s) 21
BspFNI CGCG 1 cut(s) 250
BspLI GGNNCC 3 cut(s) 771, 778, 968
BspMAI CTGCAG 1 cut(s) 375
BspPI GGATC 2 cut(s) 17, 26
BspQI GCTCTTC 2 cut(s) 163, 305
BsrDI GCAATG 1 cut(s) 207
BssECI CCNNGG 3 cut(s) 14, 42, 146
BssMI GATC 3 cut(s) 18, 22, 346
BssT1I CCWWGG 3 cut(s) 14, 42, 146
Bst2UI CCWGG 2 cut(s) 27, 774
Bst4CI ACNGT 3 cut(s) 56, 139, 244
Bst6I CTCTTC 5 cut(s) 163, 305, 366, 436, 861
BstC8I GCNNGC 3 cut(s) 194, 310, 790
BstDEI CTNAG 5 cut(s) 280, 515, 681, 814, 899
BstF5I GGATG 6 cut(s) 454, 548, 802, 847, 856, 916
BstFNI CGCG 1 cut(s) 250
BstKTI GATC 3 cut(s) 21, 25, 349
BstMAI GTCTC 2 cut(s) 122, 233
BstMBI GATC 3 cut(s) 18, 22, 346
BstMWI GCNNNNNNNGC 5 cut(s) 256, 262, 350, 488, 593
BstNI CCWGG 2 cut(s) 27, 774
BstNSI RCATGY 2 cut(s) 538, 641
BstSCI CCNGG 2 cut(s) 25, 772
BstSFI CTRYAG 2 cut(s) 371, 712
BstUI CGCG 1 cut(s) 250
BstV1I GCAGC 4 cut(s) 243, 363, 385, 406
BstV2I GAAGAC 1 cut(s) 897
Bsu15I ATCGAT 1 cut(s) 21
BsuRI GGCC 5 cut(s) 312, 477, 581, 596, 967
BsuTUI ATCGAT 1 cut(s) 21
BtgZI GCGATG 1 cut(s) 82
BtsCI GGATG 6 cut(s) 454, 548, 802, 847, 856, 916
BtsI GCAGTG 2 cut(s) 180, 368
BtsIMutI CAGTG 4 cut(s) 61, 135, 180, 368
Cac8I GCNNGC 3 cut(s) 194, 310, 790
CaiI CAGNNNCTG 4 cut(s) 179, 242, 370, 653
Cfr13I GGNCC 5 cut(s) 182, 476, 580, 770, 966
ClaI ATCGAT 1 cut(s) 21
CseI GACGC 1 cut(s) 647
CsiI ACCWGGT 1 cut(s) 772
Csp6I GTAC 3 cut(s) 538, 551, 645
CviAII CATG 6 cut(s) 162, 350, 535, 638, 837, 978
CviQI GTAC 3 cut(s) 538, 551, 645
DdeI CTNAG 5 cut(s) 280, 515, 681, 814, 899
DpnI GATC 3 cut(s) 20, 24, 348
DpnII GATC 3 cut(s) 18, 22, 346
EaeI YGGCCR 1 cut(s) 594
Eam1104I CTCTTC 5 cut(s) 163, 305, 366, 436, 861
EarI CTCTTC 5 cut(s) 163, 305, 366, 436, 861
EciI GGCGGA 1 cut(s) 222
Ecl136II GAGCTC 2 cut(s) 79, 685
Eco130I CCWWGG 3 cut(s) 14, 42, 146
Eco24I GRGCYC 2 cut(s) 81, 687
Eco47I GGWCC 2 cut(s) 182, 770
Eco53kI GAGCTC 2 cut(s) 79, 685
Eco57I CTGAAG 2 cut(s) 347, 779
Eco88I CYCGRG 1 cut(s) 751
EcoICRI GAGCTC 2 cut(s) 79, 685
EcoO109I RGGNCCY 2 cut(s) 476, 580
EcoRII CCWGG 2 cut(s) 25, 772
EcoT14I CCWWGG 3 cut(s) 14, 42, 146
EcoT22I ATGCAT 1 cut(s) 536
EcoT38I GRGCYC 2 cut(s) 81, 687
ErhI CCWWGG 3 cut(s) 14, 42, 146
FaeI CATG 6 cut(s) 165, 353, 538, 641, 840, 981
FalI AAGNNNNNCTT 4 cut(s) 415, 447, 882, 914
FaqI GGGAC 2 cut(s) 86, 783
FatI CATG 6 cut(s) 161, 349, 534, 637, 836, 977
FauI CCCGC 1 cut(s) 781
FbaI TGATCA 1 cut(s) 346
FblI GTMKAC 1 cut(s) 508
Fnu4HI GCNGC 5 cut(s) 257, 374, 377, 420, 594
FokI GGATG 6 cut(s) 461, 555, 809, 854, 863, 923
FriOI GRGCYC 2 cut(s) 81, 687
Fsp4HI GCNGC 5 cut(s) 257, 374, 377, 420, 594
GluI GCNGC 5 cut(s) 257, 374, 377, 420, 594
GsaI CCCAGC 1 cut(s) 796
GsuI CTGGAG 1 cut(s) 671
HaeIII GGCC 5 cut(s) 312, 477, 581, 596, 967
HgaI GACGC 1 cut(s) 647
Hin1II CATG 6 cut(s) 165, 353, 538, 641, 840, 981
HinfI GANTC 2 cut(s) 112, 398
Hpy166II GTNNAC 2 cut(s) 287, 509
Hpy188I TCNGA 7 cut(s) 36, 293, 366, 518, 663, 781, 808
Hpy188III TCNNGA 7 cut(s) 125, 180, 569, 670, 688, 742, 944
Hpy8I GTNNAC 2 cut(s) 287, 509
HpyAV CCTTC 2 cut(s) 131, 323
HpyCH4III ACNGT 3 cut(s) 56, 139, 244
HpyF10VI GCNNNNNNNGC 5 cut(s) 256, 262, 350, 488, 593
HpyF3I CTNAG 5 cut(s) 280, 515, 681, 814, 899
Hsp92II CATG 6 cut(s) 165, 353, 538, 641, 840, 981
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 3 cut(s) 18, 22, 346
LguI GCTCTTC 2 cut(s) 163, 305
LmnI GCTCC 2 cut(s) 219, 690
Lsp1109I GCAGC 4 cut(s) 243, 363, 385, 406
LweI GCATC 2 cut(s) 79, 521
MabI ACCWGGT 1 cut(s) 772
MaeIII GTNAC 1 cut(s) 244
MalI GATC 3 cut(s) 20, 24, 348
MboI GATC 3 cut(s) 18, 22, 346
MboII GAAGA 7 cut(s) 180, 292, 353, 423, 813, 878, 902
MhlI GDGCHC 2 cut(s) 81, 687
MluCI AATT 3 cut(s) 59, 224, 746
MmeI TCCRAC 1 cut(s) 804
Mph1103I ATGCAT 1 cut(s) 536
MspA1I CMGCKG 1 cut(s) 376
MspR9I CCNGG 2 cut(s) 27, 774
Mva1269I GAATGC 1 cut(s) 258
MvaI CCWGG 2 cut(s) 27, 774
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 5 cut(s) 256, 262, 350, 488, 593
NdeII GATC 3 cut(s) 18, 22, 346
NlaIII CATG 6 cut(s) 165, 353, 538, 641, 840, 981
NlaIV GGNNCC 3 cut(s) 771, 778, 968
NsiI ATGCAT 1 cut(s) 536
NspI RCATGY 2 cut(s) 538, 641
PciSI GCTCTTC 2 cut(s) 163, 305
PctI GAATGC 1 cut(s) 258
PfeI GAWTC 2 cut(s) 112, 398
PfoI TCCNGGA 1 cut(s) 25
PkrI GCNGC 5 cut(s) 258, 375, 378, 421, 595
Psp124BI GAGCTC 2 cut(s) 81, 687
Psp6I CCWGG 2 cut(s) 25, 772
PspFI CCCAGC 1 cut(s) 792
PspGI CCWGG 2 cut(s) 25, 772
PspN4I GGNNCC 3 cut(s) 771, 778, 968
PspPI GGNCC 5 cut(s) 182, 476, 580, 770, 966
PstI CTGCAG 1 cut(s) 375
PstNI CAGNNNCTG 4 cut(s) 179, 242, 370, 653
PvuII CAGCTG 1 cut(s) 376
RsaI GTAC 3 cut(s) 539, 552, 646
RsaNI GTAC 3 cut(s) 538, 551, 645
SacI GAGCTC 2 cut(s) 81, 687
SapI GCTCTTC 2 cut(s) 163, 305
SatI GCNGC 5 cut(s) 257, 374, 377, 420, 594
Sau3AI GATC 3 cut(s) 18, 22, 346
Sau96I GGNCC 5 cut(s) 182, 476, 580, 770, 966
ScrFI CCNGG 2 cut(s) 27, 774
SduI GDGCHC 2 cut(s) 81, 687
SexAI ACCWGGT 1 cut(s) 772
SfaNI GCATC 2 cut(s) 79, 521
SfcI CTRYAG 2 cut(s) 371, 712
SinI GGWCC 2 cut(s) 182, 770
SmlI CTYRAG 4 cut(s) 485, 567, 611, 620
SmoI CTYRAG 4 cut(s) 485, 567, 611, 620
Sse9I AATT 3 cut(s) 59, 224, 746
SsiI CCGC 4 cut(s) 212, 233, 593, 788
SstI GAGCTC 2 cut(s) 81, 687
StyD4I CCNGG 2 cut(s) 25, 772
StyI CCWWGG 3 cut(s) 14, 42, 146
TaaI ACNGT 3 cut(s) 56, 139, 244
TaqI TCGA 3 cut(s) 21, 945, 960
TasI AATT 3 cut(s) 59, 224, 746
TatI WGTACW 1 cut(s) 644
TauI GCSGC 1 cut(s) 596
TfiI GAWTC 2 cut(s) 112, 398
TscAI CASTG 4 cut(s) 61, 142, 180, 375
TseI GCWGC 4 cut(s) 256, 373, 376, 419
TspDTI ATGAA 2 cut(s) 17, 744
TspGWI ACGGA 1 cut(s) 555
TspRI CASTG 4 cut(s) 61, 142, 180, 375
VpaK11BI GGWCC 2 cut(s) 182, 770
XapI RAATTY 3 cut(s) 59, 224, 746
XceI RCATGY 2 cut(s) 538, 641
XcmI CCANNNNNNNNNTGG 1 cut(s) 442
XmiI GTMKAC 1 cut(s) 508
Zsp2I ATGCAT 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.