Rorug02G0182500

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000713
Physical Location & Seq
Reverse (-)
16971705 .. 16974623
2919 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0182500.1

Sequence Viewer

Length: 1002 bp
ATGGCGACGAATATCGGCATGATGGACGGCGCGTACTTCGTCGGCAGATCTGAGATCTTGGCCTGGATCAACTCCACTCTCCAACTCCATCTCAACAAAGTCGAAGAGGCGTGTTCCGGCGCGGTCCACTGCCAGCTCATGGACGCGGCTCATCCGGGGATGGTTCCGATGCACAAGGTCAATTTTGACGCCAAGAGCGAGTATGAGATGATCCAGAACTATAAGGTTCTTCAAGATGTCTTCAACAAACTCAAAATTACCAAGCATATTGAGGTGAACAAGCTTGTGAAAGGAAGGCCGCTCGACAATTTGGAGTTCATGCAATGGATGAAGCGCTACTGTGATTCTATCAATGGCTGCACCAGCTACAATGCTCTGGAGAGGAGAGAAGCTTGCAAGGGCGGTAAAGAAGTAAGCAAGAGAGCTGCCCAATCACAATCATCCGCTAAGGGTACAGCAACTTCCCAAAGAGCTCATTCCTCTCACAATGCTAGAAGAAATGATGTACCCCATTCTGGGAATCAATCAGTAAAGGCCTCAAGACCTTCTTCTAGTGGAGGCCCTGCCCCACCTGCATATGATGAAAAGATCACGGAGTTGAAGCTATCAGTGGATAGCCTAGAGAAGGAGAGAGATTTCTACTTTGCAAAGCTCAGAGATATTGAGATACTATGCCAGACTCCTGAAATTGAACACCTATCTGTTGTTGCTGCTATACAGAAGATTTTATATGCTACAGATGATGATGCGACAGTGGTGGCACAAGCGCAAGCCATGTTGTCTGCCCCCTCAAAGGAAGCAGAGCCCTCGAGCCCCATTGCTGAGCCATTGAGTCCCATTGCTGAGGCTTCAGATGAGAAAGCAAGTTCGGAGACCCAGAAGAGGAAAACCATAAACAACTTTGATGTCGATGCTGCCGCCATGGCAACATTATCTCCAAGGCAAAGGGTCTCTGATGCTTCTGATGTCCATTGTAGTGGGTCACCTCTGATGACTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

333

Amino Acids

36.5

Weight (kDa)

6.38

Isoelectric Point (pI)

62.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CH PF00307 16 - 108 3.9e-10 Calponin homology (CH) domain
EB1 PF03271 207 - 245 2.2e-17 EB1-like C-terminal motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 580
Acc36I ACCTGC 1 cut(s) 580
AccB7I CCANNNNNTGG 1 cut(s) 139
AccBSI CCGCTC 1 cut(s) 301
AccII CGCG 3 cut(s) 32, 122, 146
AciI CCGC 6 cut(s) 122, 146, 299, 402, 444, 918
AclWI GGATC 2 cut(s) 74, 205
AcuI CTGAAG 1 cut(s) 834
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 3 cut(s) 35, 454, 507
AfeI AGCGCT 1 cut(s) 335
AfiI CCNNNNNNNGG 6 cut(s) 139, 515, 516, 625, 793, 882
AgsI TTSAA 4 cut(s) 233, 244, 601, 692
AjnI CCWGG 1 cut(s) 62
AluBI AGCT 8 cut(s) 136, 283, 366, 392, 425, 473, 604, 652
AluI AGCT 8 cut(s) 136, 283, 366, 392, 425, 473, 604, 652
Alw21I GWGCWC 1 cut(s) 475
Alw26I GTCTC 2 cut(s) 866, 955
AlwI GGATC 2 cut(s) 74, 205
Ama87I CYCGRG 1 cut(s) 808
Aor51HI AGCGCT 1 cut(s) 335
AoxI GGCC 4 cut(s) 60, 296, 534, 559
ApeKI GCWGC 4 cut(s) 357, 425, 710, 914
AspLEI GCGC 4 cut(s) 32, 122, 336, 769
AspS9I GGNCC 2 cut(s) 124, 560
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 2 cut(s) 286, 975
AvaI CYCGRG 1 cut(s) 808
AvaII GGWCC 1 cut(s) 124
BanII GRGCYC 3 cut(s) 475, 807, 815
BarI GAAGNNNNNNTAC 2 cut(s) 445, 477
BbsI GAAGAC 1 cut(s) 232
Bbv12I GWGCWC 1 cut(s) 475
BbvCI CCTCAGC 1 cut(s) 843
BbvI GCAGC 4 cut(s) 344, 412, 697, 901
BccI CCATC 3 cut(s) 16, 96, 154
BceAI ACGGC 1 cut(s) 43
BcgI CGANNNNNNTGC 2 cut(s) 789, 823
BciT130I CCWGG 1 cut(s) 64
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 2 cut(s) 866, 955
BfaI CTAG 3 cut(s) 492, 552, 620
BfmI CTRYAG 1 cut(s) 735
BfoI RGCGCY 1 cut(s) 337
BfuAI ACCTGC 1 cut(s) 580
BglI GCCNNNNNGGC 1 cut(s) 923
BglII AGATCT 2 cut(s) 47, 54
BisI GCNGC 7 cut(s) 147, 299, 358, 426, 711, 915, 918
BlpI GCTNAGC 1 cut(s) 822
BlsI GCNGC 7 cut(s) 148, 300, 359, 427, 712, 916, 919
Bme1390I CCNGG 2 cut(s) 64, 156
Bme18I GGWCC 1 cut(s) 124
BmeT110I CYCGRG 1 cut(s) 808
BmgT120I GGNCC 2 cut(s) 124, 560
BmiI GGNNCC 1 cut(s) 165
BmrFI CCNGG 2 cut(s) 64, 156
BmsI GCATC 4 cut(s) 159, 736, 901, 946
BpiI GAAGAC 1 cut(s) 232
BpmI CTGGAG 1 cut(s) 398
Bpu10I CCTNAGC 2 cut(s) 447, 843
Bpu1102I GCTNAGC 1 cut(s) 822
BpuEI CTTGAG 1 cut(s) 523
BpuMI CCSGG 1 cut(s) 156
BsaHI GRCGYC 1 cut(s) 189
BsaI GGTCTC 2 cut(s) 866, 955
BsaJI CCNNGG 3 cut(s) 155, 921, 938
BsaXI ACNNNNNCTCC 2 cut(s) 919, 949
Bsc4I CCNNNNNNNGG 6 cut(s) 139, 515, 516, 625, 793, 882
Bse3DI GCAATG 3 cut(s) 329, 816, 837
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 3 cut(s) 155, 921, 938
BseGI GGATG 4 cut(s) 151, 165, 333, 440
BseLI CCNNNNNNNGG 6 cut(s) 139, 515, 516, 625, 793, 882
BseMI GCAATG 3 cut(s) 329, 816, 837
BseMII CTCAG 4 cut(s) 42, 667, 813, 834
BseRI GAGGAG 1 cut(s) 397
BseXI GCAGC 4 cut(s) 344, 412, 697, 901
BsgI GTGCAG 1 cut(s) 343
Bsh1236I CGCG 3 cut(s) 32, 122, 146
BshFI GGCC 4 cut(s) 62, 298, 536, 561
BsiHKAI GWGCWC 1 cut(s) 475
BsiHKCI CYCGRG 1 cut(s) 808
BsiSI CCGG 2 cut(s) 117, 155
BslFI GGGAC 1 cut(s) 819
BslI CCNNNNNNNGG 6 cut(s) 139, 515, 516, 625, 793, 882
BsmAI GTCTC 2 cut(s) 866, 955
BsmFI GGGAC 1 cut(s) 819
BsnI GGCC 4 cut(s) 62, 298, 536, 561
Bso31I GGTCTC 2 cut(s) 866, 955
BsoBI CYCGRG 1 cut(s) 808
Bsp1286I GDGCHC 3 cut(s) 475, 807, 815
Bsp143I GATC 5 cut(s) 47, 54, 66, 210, 588
Bsp1720I GCTNAGC 1 cut(s) 822
Bsp19I CCATGG 1 cut(s) 921
BspACI CCGC 6 cut(s) 122, 146, 299, 402, 444, 918
BspANI GGCC 4 cut(s) 62, 298, 536, 561
BspCNI CTCAG 4 cut(s) 43, 666, 814, 835
BspFNI CGCG 3 cut(s) 32, 122, 146
BspLI GGNNCC 1 cut(s) 165
BspMI ACCTGC 1 cut(s) 580
BspPI GGATC 2 cut(s) 74, 205
BspTNI GGTCTC 2 cut(s) 866, 955
BsrBI CCGCTC 1 cut(s) 301
BsrDI GCAATG 3 cut(s) 329, 816, 837
BssECI CCNNGG 3 cut(s) 155, 921, 938
BssMI GATC 5 cut(s) 47, 54, 66, 210, 588
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 2 cut(s) 921, 938
Bst2UI CCWGG 1 cut(s) 64
Bst4CI ACNGT 2 cut(s) 341, 754
Bst6I CTCTTC 2 cut(s) 99, 875
BstACI GRCGYC 1 cut(s) 189
BstC8I GCNNGC 3 cut(s) 134, 394, 771
BstDEI CTNAG 5 cut(s) 51, 447, 653, 822, 843
BstDSI CCRYGG 1 cut(s) 921
BstEII GGTNACC 1 cut(s) 981
BstENI CCTNNNNNAGG 1 cut(s) 623
BstF5I GGATG 4 cut(s) 151, 165, 333, 440
BstFNI CGCG 3 cut(s) 32, 122, 146
BstH2I RGCGCY 1 cut(s) 337
BstHHI GCGC 4 cut(s) 32, 122, 336, 769
BstKTI GATC 5 cut(s) 50, 57, 69, 213, 591
BstMAI GTCTC 2 cut(s) 866, 955
BstMBI GATC 5 cut(s) 47, 54, 66, 210, 588
BstMWI GCNNNNNNNGC 3 cut(s) 363, 572, 923
BstNI CCWGG 1 cut(s) 64
BstPI GGTNACC 1 cut(s) 981
BstSCI CCNGG 2 cut(s) 62, 154
BstSFI CTRYAG 1 cut(s) 735
BstUI CGCG 3 cut(s) 32, 122, 146
BstV1I GCAGC 4 cut(s) 344, 412, 697, 901
BstV2I GAAGAC 1 cut(s) 232
BstX2I RGATCY 2 cut(s) 47, 54
BstXI CCANNNNNNTGG 1 cut(s) 977
BstYI RGATCY 2 cut(s) 47, 54
BsuRI GGCC 4 cut(s) 62, 298, 536, 561
BtgI CCRYGG 1 cut(s) 921
BtsCI GGATG 4 cut(s) 151, 165, 333, 440
BtsI GCAGTG 1 cut(s) 127
BtsIMutI CAGTG 3 cut(s) 127, 615, 759
BveI ACCTGC 1 cut(s) 580
Cac8I GCNNGC 3 cut(s) 134, 394, 771
CfoI GCGC 4 cut(s) 32, 122, 336, 769
Cfr13I GGNCC 2 cut(s) 124, 560
CseI GACGC 2 cut(s) 152, 197
Csp6I GTAC 3 cut(s) 34, 453, 506
CviAII CATG 5 cut(s) 19, 139, 319, 775, 922
CviQI GTAC 3 cut(s) 34, 453, 506
DdeI CTNAG 5 cut(s) 51, 447, 653, 822, 843
DpnI GATC 5 cut(s) 49, 56, 68, 212, 590
DpnII GATC 5 cut(s) 47, 54, 66, 210, 588
Eam1104I CTCTTC 2 cut(s) 99, 875
EarI CTCTTC 2 cut(s) 99, 875
Ecl136II GAGCTC 1 cut(s) 473
Eco130I CCWWGG 2 cut(s) 921, 938
Eco147I AGGCCT 1 cut(s) 536
Eco24I GRGCYC 3 cut(s) 475, 807, 815
Eco31I GGTCTC 2 cut(s) 866, 955
Eco47I GGWCC 1 cut(s) 124
Eco47III AGCGCT 1 cut(s) 335
Eco53kI GAGCTC 1 cut(s) 473
Eco57I CTGAAG 1 cut(s) 834
Eco88I CYCGRG 1 cut(s) 808
Eco91I GGTNACC 1 cut(s) 981
EcoICRI GAGCTC 1 cut(s) 473
EcoNI CCTNNNNNAGG 1 cut(s) 623
EcoO109I RGGNCCY 1 cut(s) 560
EcoO65I GGTNACC 1 cut(s) 981
EcoRII CCWGG 1 cut(s) 62
EcoT14I CCWWGG 2 cut(s) 921, 938
EcoT38I GRGCYC 3 cut(s) 475, 807, 815
ErhI CCWWGG 2 cut(s) 921, 938
FaeI CATG 5 cut(s) 22, 142, 322, 778, 925
FalI AAGNNNNNCTT 2 cut(s) 532, 564
FaqI GGGAC 1 cut(s) 819
FatI CATG 5 cut(s) 18, 138, 318, 774, 921
FauNDI CATATG 1 cut(s) 577
Fnu4HI GCNGC 7 cut(s) 147, 299, 358, 426, 711, 915, 918
FokI GGATG 4 cut(s) 138, 172, 340, 427
FriOI GRGCYC 3 cut(s) 475, 807, 815
Fsp4HI GCNGC 7 cut(s) 147, 299, 358, 426, 711, 915, 918
FspBI CTAG 3 cut(s) 492, 552, 620
GlaI GCGC 4 cut(s) 31, 121, 335, 768
GluI GCNGC 7 cut(s) 147, 299, 358, 426, 711, 915, 918
GsuI CTGGAG 1 cut(s) 398
HaeII RGCGCY 1 cut(s) 337
HaeIII GGCC 4 cut(s) 62, 298, 536, 561
HapII CCGG 2 cut(s) 117, 155
HgaI GACGC 2 cut(s) 152, 197
HhaI GCGC 4 cut(s) 32, 122, 336, 769
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 5 cut(s) 22, 142, 322, 778, 925
Hin6I GCGC 4 cut(s) 30, 120, 334, 767
HinP1I GCGC 4 cut(s) 30, 120, 334, 767
HindIII AAGCTT 2 cut(s) 281, 390
HinfI GANTC 4 cut(s) 344, 520, 679, 832
HpaII CCGG 2 cut(s) 117, 155
HphI GGTGA 2 cut(s) 286, 975
Hpy166II GTNNAC 2 cut(s) 127, 277
Hpy188I TCNGA 8 cut(s) 52, 168, 656, 853, 871, 955, 964, 990
Hpy188III TCNNGA 5 cut(s) 214, 233, 377, 540, 683
Hpy8I GTNNAC 2 cut(s) 127, 277
Hpy99I CGWCG 2 cut(s) 10, 44
HpyAV CCTTC 3 cut(s) 288, 555, 619
HpyCH4III ACNGT 2 cut(s) 341, 754
HpyCH4V TGCA 6 cut(s) 172, 322, 360, 396, 575, 647
HpyF10VI GCNNNNNNNGC 3 cut(s) 363, 572, 923
HpyF3I CTNAG 5 cut(s) 51, 447, 653, 822, 843
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 5 cut(s) 22, 142, 322, 778, 925
HspAI GCGC 4 cut(s) 30, 120, 334, 767
Kzo9I GATC 5 cut(s) 47, 54, 66, 210, 588
Lsp1109I GCAGC 4 cut(s) 344, 412, 697, 901
LweI GCATC 4 cut(s) 159, 736, 901, 946
MaeI CTAG 3 cut(s) 492, 552, 620
MaeIII GTNAC 1 cut(s) 981
MalI GATC 5 cut(s) 49, 56, 68, 212, 590
MbiI CCGCTC 1 cut(s) 301
MboI GATC 5 cut(s) 47, 54, 66, 210, 588
MboII GAAGA 7 cut(s) 116, 221, 232, 507, 540, 733, 892
MflI RGATCY 2 cut(s) 47, 54
MhlI GDGCHC 3 cut(s) 475, 807, 815
MluCI AATT 4 cut(s) 181, 255, 307, 687
MlyI GAGTC 2 cut(s) 673, 841
MmeI TCCRAC 1 cut(s) 106
MslI CAYNNNNRTG 1 cut(s) 975
MspI CCGG 2 cut(s) 117, 155
MspR9I CCNGG 2 cut(s) 64, 156
MvaI CCWGG 1 cut(s) 64
MvnI CGCG 3 cut(s) 32, 122, 146
MwoI GCNNNNNNNGC 3 cut(s) 363, 572, 923
NciI CCSGG 1 cut(s) 156
NcoI CCATGG 1 cut(s) 921
NdeI CATATG 1 cut(s) 577
NdeII GATC 5 cut(s) 47, 54, 66, 210, 588
NlaIII CATG 5 cut(s) 22, 142, 322, 778, 925
NlaIV GGNNCC 1 cut(s) 165
NmuCI GTSAC 1 cut(s) 981
PaeR7I CTCGAG 1 cut(s) 808
PaqCI CACCTGC 1 cut(s) 580
PceI AGGCCT 1 cut(s) 536
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PfeI GAWTC 2 cut(s) 344, 520
PflMI CCANNNNNTGG 1 cut(s) 139
PkrI GCNGC 7 cut(s) 148, 300, 359, 427, 712, 916, 919
PleI GAGTC 2 cut(s) 673, 840
PpsI GAGTC 2 cut(s) 673, 840
Psp124BI GAGCTC 1 cut(s) 475
Psp6I CCWGG 1 cut(s) 62
PspEI GGTNACC 1 cut(s) 981
PspGI CCWGG 1 cut(s) 62
PspN4I GGNNCC 1 cut(s) 165
PspPI GGNCC 2 cut(s) 124, 560
PspXI VCTCGAGB 1 cut(s) 808
PsuI RGATCY 2 cut(s) 47, 54
RsaI GTAC 3 cut(s) 35, 454, 507
RsaNI GTAC 3 cut(s) 34, 453, 506
RseI CAYNNNNRTG 1 cut(s) 975
SacI GAGCTC 1 cut(s) 475
SatI GCNGC 7 cut(s) 147, 299, 358, 426, 711, 915, 918
Sau3AI GATC 5 cut(s) 47, 54, 66, 210, 588
Sau96I GGNCC 2 cut(s) 124, 560
SchI GAGTC 2 cut(s) 673, 841
ScrFI CCNGG 2 cut(s) 64, 156
SduI GDGCHC 3 cut(s) 475, 807, 815
SfaNI GCATC 4 cut(s) 159, 736, 901, 946
SfcI CTRYAG 1 cut(s) 735
Sfr274I CTCGAG 1 cut(s) 808
SinI GGWCC 1 cut(s) 124
SlaI CTCGAG 1 cut(s) 808
SmiMI CAYNNNNRTG 1 cut(s) 975
SmlI CTYRAG 2 cut(s) 538, 808
SmoI CTYRAG 2 cut(s) 538, 808
Sse9I AATT 4 cut(s) 181, 255, 307, 687
SseBI AGGCCT 1 cut(s) 536
SsiI CCGC 6 cut(s) 122, 146, 299, 402, 444, 918
SspMI CTAG 3 cut(s) 492, 552, 620
SstI GAGCTC 1 cut(s) 475
StuI AGGCCT 1 cut(s) 536
StyD4I CCNGG 2 cut(s) 62, 154
StyI CCWWGG 2 cut(s) 921, 938
TaaI ACNGT 2 cut(s) 341, 754
TaqI TCGA 4 cut(s) 102, 303, 809, 909
TasI AATT 4 cut(s) 181, 255, 307, 687
TauI GCSGC 3 cut(s) 149, 301, 920
TfiI GAWTC 2 cut(s) 344, 520
TscAI CASTG 3 cut(s) 134, 615, 759
TseFI GTSAC 1 cut(s) 981
TseI GCWGC 4 cut(s) 357, 425, 710, 914
Tsp45I GTSAC 1 cut(s) 981
TspDTI ATGAA 3 cut(s) 307, 344, 597
TspGWI ACGGA 1 cut(s) 608
TspRI CASTG 3 cut(s) 134, 615, 759
Van91I CCANNNNNTGG 1 cut(s) 139
VpaK11BI GGWCC 1 cut(s) 124
XagI CCTNNNNNAGG 1 cut(s) 623
XhoI CTCGAG 1 cut(s) 808
XspI CTAG 3 cut(s) 492, 552, 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.