Rorug02G0182300

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000713
Physical Location & Seq
Forward (+)
16969142 .. 16970846
1705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0182300.1

Sequence Viewer

Length: 915 bp
ATGGACATGGAAGCCCAGAGCTTTTTATGGAAAGGAGCAATTCCTTTGCAGATTCATCTCTATGAATCCGAAGTCACCACTCTTCCTTCTCCCCCACCCGCCCTGATCTTAGCTCCTCGGCTAGGATACCTACCGTTGTTGGCCTCGCATTTAAAGCCTTACTTCAGCAGTGCACTTCCTCCTGGTGTTGACACTATTTGGTTCGAGTACAAAGGCTTGCCCTTGAAATGGTATATACCAACAGGAGTTCTTTTTGATCTTTTATGTGCAGAAGCAGAGAGACCTTGGAATTTAACGGTGCATTTTAGAGGATATCCAGGAAACATATTGATCCCTTGTGAAGGTGAAGACAGCATAAAGTGGAGCTTTATCAACTCATTGAAAGAGGCAGCATATATAATAAATGGAAACTGCAAGAATGTGATGAACATGTCTCAACCTGATCAGGTGGACCTCTGGCGCTCTGTTTTACATGGTAATTTCGAAGCCTACCAACGGGTATCTTCTAAACTTAAGCTTGGAGCAGTTGGGGAAATTGATGTTTCTGGGCAGGTGAAGCAAGGCCGAGTTCCAGTTCGGTTATACGTTTGGAATGTCAGTGACGACTTTGATGACTTAGAAGATGCACCACCGATTGATAGTTGGGACAAAGTTTCTTACATAAACCGGCCTGTCGAGATACAAGGAGAAGGTAGATGCTTCACTTTATATGATGCAGTGAAAAGCCTTCTGCCGGACTATTTTGTGGACAAATCGTTGGGCAATACTGAATCATTGACAGTAGGTCGCGATGATGACGAGCCTAAAATCAAGCTGGTTCGTATACAAGACCGAAGAGTGGATGCCACAGCTGCGGCAGCACCGGTCAGAACTCCCCCGCTCGTATTTGTAATCGGCGAGGGATGTGACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

304

Amino Acids

34.14

Weight (kDa)

4.9

Isoelectric Point (pI)

34.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG5_UblA PF20638 9 - 102 5.8e-29 Autophagy protein ATG5, UblA domain
ATG5_HBR PF20637 116 - 172 1.1e-23 Autophagy protein ATG5, alpha-helical bundle region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 541
Acc36I ACCTGC 1 cut(s) 541
AccBSI CCGCTC 1 cut(s) 880
AccI GTMKAC 1 cut(s) 823
AccII CGCG 1 cut(s) 789
AciI CCGC 3 cut(s) 99, 854, 878
AclWI GGATC 1 cut(s) 325
AcsI RAATTY 1 cut(s) 289
AcuI CTGAAG 1 cut(s) 148
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 6 cut(s) 122, 228, 341, 495, 733, 838
AflII CTTAAG 1 cut(s) 512
AflIII ACRYGT 1 cut(s) 429
AgeI ACCGGT 1 cut(s) 862
AgsI TTSAA 2 cut(s) 226, 382
AhdI GACNNNNNGTC 1 cut(s) 783
AjnI CCWGG 2 cut(s) 181, 316
AluBI AGCT 6 cut(s) 21, 113, 366, 517, 814, 851
AluI AGCT 6 cut(s) 21, 113, 366, 517, 814, 851
Alw21I GWGCWC 1 cut(s) 175
Alw26I GTCTC 2 cut(s) 274, 438
Alw44I GTGCAC 1 cut(s) 171
AlwI GGATC 1 cut(s) 325
AoxI GGCC 3 cut(s) 141, 562, 668
ApaLI GTGCAC 1 cut(s) 171
ApeKI GCWGC 3 cut(s) 389, 851, 857
ApoI RAATTY 1 cut(s) 289
AsiGI ACCGGT 1 cut(s) 862
AspLEI GCGC 1 cut(s) 462
AspS9I GGNCC 1 cut(s) 451
AsuHPI GGTGA 3 cut(s) 67, 356, 565
AsuII TTCGAA 1 cut(s) 483
AvaII GGWCC 1 cut(s) 451
BaeGI GKGCMC 1 cut(s) 175
BbsI GAAGAC 1 cut(s) 354
Bbv12I GWGCWC 1 cut(s) 175
BbvI GCAGC 3 cut(s) 401, 838, 869
BciT130I CCWGG 2 cut(s) 183, 318
BciVI GTATCC 1 cut(s) 119
BclI TGATCA 1 cut(s) 442
BcoDI GTCTC 2 cut(s) 274, 438
BfaI CTAG 1 cut(s) 122
BfoI RGCGCY 1 cut(s) 463
BfrI CTTAAG 1 cut(s) 512
BfuAI ACCTGC 1 cut(s) 541
BfuI GTATCC 1 cut(s) 119
BisI GCNGC 4 cut(s) 390, 852, 855, 858
BlsI GCNGC 4 cut(s) 391, 853, 856, 859
Bme1390I CCNGG 2 cut(s) 183, 318
Bme18I GGWCC 1 cut(s) 451
BmeRI GACNNNNNGTC 1 cut(s) 783
BmgT120I GGNCC 1 cut(s) 451
BmrFI CCNGG 2 cut(s) 183, 318
BmsI GCATC 4 cut(s) 613, 686, 703, 832
BpiI GAAGAC 1 cut(s) 354
Bpu14I TTCGAA 1 cut(s) 483
BsaI GGTCTC 1 cut(s) 274
BsaJI CCNNGG 2 cut(s) 116, 284
BsaWI WCCGGW 1 cut(s) 862
Bsc4I CCNNNNNNNGG 6 cut(s) 122, 228, 341, 495, 733, 838
Bse118I RCCGGY 2 cut(s) 666, 862
Bse1I ACTGG 2 cut(s) 572, 914
BseBI CCWGG 2 cut(s) 183, 318
BseDI CCNNGG 2 cut(s) 116, 284
BseGI GGATG 2 cut(s) 847, 908
BseLI CCNNNNNNNGG 6 cut(s) 122, 228, 341, 495, 733, 838
BseNI ACTGG 2 cut(s) 572, 914
BseRI GAGGAG 1 cut(s) 105
BseSI GKGCMC 1 cut(s) 175
BseXI GCAGC 3 cut(s) 401, 838, 869
BsgI GTGCAG 1 cut(s) 288
Bsh1236I CGCG 1 cut(s) 789
BshFI GGCC 3 cut(s) 143, 564, 670
BshTI ACCGGT 1 cut(s) 862
BsiHKAI GWGCWC 1 cut(s) 175
BsiSI CCGG 3 cut(s) 667, 734, 863
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 6 cut(s) 122, 228, 341, 495, 733, 838
BsmAI GTCTC 2 cut(s) 274, 438
BsmFI GGGAC 1 cut(s) 659
BsnI GGCC 3 cut(s) 143, 564, 670
Bso31I GGTCTC 1 cut(s) 274
Bsp119I TTCGAA 1 cut(s) 483
Bsp1286I GDGCHC 1 cut(s) 175
Bsp143I GATC 4 cut(s) 105, 256, 330, 442
Bsp68I TCGCGA 1 cut(s) 789
BspACI CCGC 3 cut(s) 99, 854, 878
BspANI GGCC 3 cut(s) 143, 564, 670
BspFNI CGCG 1 cut(s) 789
BspMI ACCTGC 1 cut(s) 541
BspPI GGATC 1 cut(s) 325
BspT104I TTCGAA 1 cut(s) 483
BspTI CTTAAG 1 cut(s) 512
BspTNI GGTCTC 1 cut(s) 274
BsrBI CCGCTC 1 cut(s) 880
BsrFI RCCGGY 2 cut(s) 666, 862
BsrI ACTGG 2 cut(s) 572, 914
BssAI RCCGGY 2 cut(s) 666, 862
BssECI CCNNGG 2 cut(s) 116, 284
BssMI GATC 4 cut(s) 105, 256, 330, 442
BssNAI GTATAC 1 cut(s) 824
BssT1I CCWWGG 1 cut(s) 284
Bst1107I GTATAC 1 cut(s) 824
Bst2UI CCWGG 2 cut(s) 183, 318
Bst4CI ACNGT 3 cut(s) 135, 298, 781
Bst6I CTCTTC 2 cut(s) 87, 829
BstAFI CTTAAG 1 cut(s) 512
BstBI TTCGAA 1 cut(s) 483
BstC8I GCNNGC 1 cut(s) 218
BstDEI CTNAG 2 cut(s) 109, 616
BstENI CCTNNNNNAGG 2 cut(s) 120, 339
BstF5I GGATG 2 cut(s) 847, 908
BstFNI CGCG 1 cut(s) 789
BstH2I RGCGCY 1 cut(s) 463
BstHHI GCGC 1 cut(s) 462
BstKTI GATC 4 cut(s) 108, 259, 333, 445
BstMAI GTCTC 2 cut(s) 274, 438
BstMBI GATC 4 cut(s) 105, 256, 330, 442
BstMWI GCNNNNNNNGC 4 cut(s) 154, 556, 851, 857
BstNI CCWGG 2 cut(s) 183, 318
BstNSI RCATGY 1 cut(s) 433
BstSCI CCNGG 2 cut(s) 181, 316
BstSLI GKGCMC 1 cut(s) 175
BstUI CGCG 1 cut(s) 789
BstV1I GCAGC 3 cut(s) 401, 838, 869
BstV2I GAAGAC 1 cut(s) 354
BstZ17I GTATAC 1 cut(s) 824
BsuI GTATCC 1 cut(s) 119
BsuRI GGCC 3 cut(s) 143, 564, 670
BtgZI GCGATG 1 cut(s) 804
BtsCI GGATG 2 cut(s) 847, 908
BtsI GCAGTG 2 cut(s) 175, 723
BtsIMutI CAGTG 3 cut(s) 175, 604, 723
BtuMI TCGCGA 1 cut(s) 789
BveI ACCTGC 1 cut(s) 541
Cac8I GCNNGC 1 cut(s) 218
CfoI GCGC 1 cut(s) 462
Cfr10I RCCGGY 2 cut(s) 666, 862
Cfr13I GGNCC 1 cut(s) 451
Csp6I GTAC 1 cut(s) 208
CspAI ACCGGT 1 cut(s) 862
CviAII CATG 3 cut(s) 7, 430, 473
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 2 cut(s) 109, 616
DpnI GATC 4 cut(s) 107, 258, 332, 444
DpnII GATC 4 cut(s) 105, 256, 330, 442
DraI TTTAAA 1 cut(s) 153
DriI GACNNNNNGTC 1 cut(s) 783
Eam1104I CTCTTC 2 cut(s) 87, 829
Eam1105I GACNNNNNGTC 1 cut(s) 783
EarI CTCTTC 2 cut(s) 87, 829
Eco130I CCWWGG 1 cut(s) 284
Eco31I GGTCTC 1 cut(s) 274
Eco32I GATATC 1 cut(s) 314
Eco47I GGWCC 1 cut(s) 451
Eco57I CTGAAG 1 cut(s) 148
EcoNI CCTNNNNNAGG 2 cut(s) 120, 339
EcoRII CCWGG 2 cut(s) 181, 316
EcoRV GATATC 1 cut(s) 314
EcoT14I CCWWGG 1 cut(s) 284
ErhI CCWWGG 1 cut(s) 284
FaeI CATG 3 cut(s) 10, 433, 476
FalI AAGNNNNNCTT 4 cut(s) 146, 178, 350, 382
FaqI GGGAC 1 cut(s) 659
FatI CATG 3 cut(s) 6, 429, 472
FauI CCCGC 2 cut(s) 106, 885
FbaI TGATCA 1 cut(s) 442
FblI GTMKAC 1 cut(s) 823
Fnu4HI GCNGC 4 cut(s) 390, 852, 855, 858
FokI GGATG 1 cut(s) 854
Fsp4HI GCNGC 4 cut(s) 390, 852, 855, 858
FspBI CTAG 1 cut(s) 122
GlaI GCGC 1 cut(s) 461
GluI GCNGC 4 cut(s) 390, 852, 855, 858
HaeII RGCGCY 1 cut(s) 463
HaeIII GGCC 3 cut(s) 143, 564, 670
HapII CCGG 3 cut(s) 667, 734, 863
HhaI GCGC 1 cut(s) 462
Hin1II CATG 3 cut(s) 10, 433, 476
Hin6I GCGC 1 cut(s) 460
HinP1I GCGC 1 cut(s) 460
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 3 cut(s) 52, 65, 770
HpaII CCGG 3 cut(s) 667, 734, 863
HphI GGTGA 3 cut(s) 67, 356, 565
Hpy166II GTNNAC 5 cut(s) 173, 190, 451, 748, 824
Hpy188I TCNGA 2 cut(s) 70, 869
Hpy188III TCNNGA 2 cut(s) 676, 788
Hpy8I GTNNAC 5 cut(s) 173, 190, 451, 748, 824
HpyAV CCTTC 4 cut(s) 96, 335, 683, 737
HpyCH4III ACNGT 3 cut(s) 135, 298, 781
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 7 cut(s) 49, 173, 269, 301, 414, 626, 716
HpyF10VI GCNNNNNNNGC 4 cut(s) 154, 556, 851, 857
HpyF3I CTNAG 2 cut(s) 109, 616
HpySE526I ACGT 1 cut(s) 585
Hsp92II CATG 3 cut(s) 10, 433, 476
HspAI GCGC 1 cut(s) 460
Ksp22I TGATCA 1 cut(s) 442
Kzo9I GATC 4 cut(s) 105, 256, 330, 442
LmnI GCTCC 4 cut(s) 35, 118, 363, 521
Lsp1109I GCAGC 3 cut(s) 401, 838, 869
LweI GCATC 4 cut(s) 613, 686, 703, 832
MaeI CTAG 1 cut(s) 122
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 3 cut(s) 73, 599, 905
MalI GATC 4 cut(s) 107, 258, 332, 444
MbiI CCGCTC 1 cut(s) 880
MboI GATC 4 cut(s) 105, 256, 330, 442
MboII GAAGA 5 cut(s) 74, 359, 495, 632, 846
MhlI GDGCHC 1 cut(s) 175
MluCI AATT 4 cut(s) 39, 289, 478, 534
MnlI CCTC 7 cut(s) 126, 154, 189, 302, 379, 464, 892
MseI TTAA 3 cut(s) 152, 293, 513
MslI CAYNNNNRTG 1 cut(s) 60
MspA1I CMGCKG 1 cut(s) 851
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 3 cut(s) 667, 734, 863
MspR9I CCNGG 2 cut(s) 183, 318
MvaI CCWGG 2 cut(s) 183, 318
MvnI CGCG 1 cut(s) 789
MwoI GCNNNNNNNGC 4 cut(s) 154, 556, 851, 857
NdeII GATC 4 cut(s) 105, 256, 330, 442
NlaIII CATG 3 cut(s) 10, 433, 476
NmeAIII GCCGAG 2 cut(s) 97, 590
NmuCI GTSAC 3 cut(s) 73, 599, 905
NruI TCGCGA 1 cut(s) 789
NspI RCATGY 1 cut(s) 433
NspV TTCGAA 1 cut(s) 483
PaqCI CACCTGC 1 cut(s) 541
PciI ACATGT 1 cut(s) 429
PfeI GAWTC 3 cut(s) 52, 65, 770
PfoI TCCNGGA 1 cut(s) 316
PinAI ACCGGT 1 cut(s) 862
PkrI GCNGC 4 cut(s) 391, 853, 856, 859
PscI ACATGT 1 cut(s) 429
Psp6I CCWGG 2 cut(s) 181, 316
PspGI CCWGG 2 cut(s) 181, 316
PspPI GGNCC 1 cut(s) 451
PvuII CAGCTG 1 cut(s) 851
RruI TCGCGA 1 cut(s) 789
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 60
SaqAI TTAA 3 cut(s) 152, 293, 513
SatI GCNGC 4 cut(s) 390, 852, 855, 858
Sau3AI GATC 4 cut(s) 105, 256, 330, 442
Sau96I GGNCC 1 cut(s) 451
ScrFI CCNGG 2 cut(s) 183, 318
SduI GDGCHC 1 cut(s) 175
SfaNI GCATC 4 cut(s) 613, 686, 703, 832
SfuI TTCGAA 1 cut(s) 483
SinI GGWCC 1 cut(s) 451
SmiMI CAYNNNNRTG 1 cut(s) 60
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
Sse9I AATT 4 cut(s) 39, 289, 478, 534
SsiI CCGC 3 cut(s) 99, 854, 878
SspMI CTAG 1 cut(s) 122
StyD4I CCNGG 2 cut(s) 181, 316
StyI CCWWGG 1 cut(s) 284
TaaI ACNGT 3 cut(s) 135, 298, 781
TaiI ACGT 1 cut(s) 588
TaqI TCGA 3 cut(s) 204, 483, 675
TaqII GACCGA 1 cut(s) 846
TasI AATT 4 cut(s) 39, 289, 478, 534
TatI WGTACW 1 cut(s) 207
TauI GCSGC 1 cut(s) 857
TfiI GAWTC 3 cut(s) 52, 65, 770
Tru1I TTAA 3 cut(s) 152, 293, 513
Tru9I TTAA 3 cut(s) 152, 293, 513
TscAI CASTG 3 cut(s) 175, 604, 723
TseFI GTSAC 3 cut(s) 73, 599, 905
TseI GCWGC 3 cut(s) 389, 851, 857
Tsp45I GTSAC 3 cut(s) 73, 599, 905
TspDTI ATGAA 3 cut(s) 44, 78, 440
TspRI CASTG 3 cut(s) 175, 604, 723
Vha464I CTTAAG 1 cut(s) 512
VneI GTGCAC 1 cut(s) 171
VpaK11BI GGWCC 1 cut(s) 451
XagI CCTNNNNNAGG 2 cut(s) 120, 339
XapI RAATTY 1 cut(s) 289
XceI RCATGY 1 cut(s) 433
XmiI GTMKAC 1 cut(s) 823
XspI CTAG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.