pycom03g22540

Desiccation-related protein PCC13-62-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001867
Physical Location & Seq
Forward (+)
22636195 .. 22637838
1644 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
ATGAAAATAGCAATAATTACCAGATTAGGAGGATTTCCAAGGCCTCAGTTGGACCTGAGCCGTGAGAATTTCGCCCGACTAATGGATGCAGCGGTGGGTTACCCGTTGAACCCTCCATTTAACATCTATGCAAGTTCAATCAATTTTCTCTTAGGATCTTATTGCATCCCTCATGTGGGACTAAATGGTTATGTTGGCACCATTCCAAACCTCACAAGGCCTGATAACCGCAGATTGGTTGCGTCACTTATGGGGGTTGAGGCTGGACAAGATGCAGTGATTCGGGCGTTACTGTATAAGTTCGCTTTTTTGAAGGTGTATCCGTACGAGCTCACGGTGGCTGATTTCACAAACCACATTTCCTGGCTGAGGAACGAGCTGGGGAGGTGTGGCAACAAAGATGAAGGAATCATAGTACCCGAGACGTTAGGGGCGGAGAACCGGACGTGGAGTAACGTCCTTTCGGCAGATTGGGAGTCACTGACGTATGCAAGGACGCAGCCGGAGATCTTGAGGATTGTGTATGCAACTGGTAATGAGTCCTGGCCCGGAGGATTTTTTCCCAGAGGTGCAAATGGCAACATTGCCATGCGTTTCCTTAAAAACAATATTATTGCAGATCAGAATAATTTACAAGAGGCAACTAAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ferritin_2

Protein Analysis

218

Amino Acids

24.23

Weight (kDa)

8.55

Isoelectric Point (pI)

31.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferritin_2 PF13668 11 - 100 6.9e-15 Ferritin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 197
AciI CCGC 3 cut(s) 92, 229, 434
AclWI GGATC 1 cut(s) 163
AcsI RAATTY 1 cut(s) 67
AfaI GTAC 2 cut(s) 326, 417
AfiI CCNNNNNNNGG 5 cut(s) 82, 175, 176, 235, 369
AgsI TTSAA 3 cut(s) 109, 138, 313
AjiI CACGTC 1 cut(s) 447
AjnI CCWGG 2 cut(s) 362, 542
AluBI AGCT 2 cut(s) 331, 379
AluI AGCT 2 cut(s) 331, 379
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 1 cut(s) 416
AlwI GGATC 1 cut(s) 163
Ama87I CYCGRG 1 cut(s) 419
AoxI GGCC 3 cut(s) 41, 218, 545
ApeKI GCWGC 2 cut(s) 89, 499
ApoI RAATTY 1 cut(s) 67
AspS9I GGNCC 2 cut(s) 52, 546
AsuC2I CCSGG 1 cut(s) 549
AvaI CYCGRG 1 cut(s) 419
AvaII GGWCC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 197
BanII GRGCYC 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 333
BbvCI CCTCAGC 1 cut(s) 368
BbvI GCAGC 2 cut(s) 101, 511
BceAI ACGGC 1 cut(s) 45
BciT130I CCWGG 2 cut(s) 364, 544
BciVI GTATCC 1 cut(s) 330
BcnI CCSGG 1 cut(s) 549
BcoDI GTCTC 1 cut(s) 416
BfuI GTATCC 1 cut(s) 330
BglII AGATCT 1 cut(s) 507
BisI GCNGC 2 cut(s) 90, 500
BlsI GCNGC 2 cut(s) 91, 501
Bme1390I CCNGG 3 cut(s) 364, 544, 549
Bme18I GGWCC 1 cut(s) 52
BmeT110I CYCGRG 1 cut(s) 419
BmgBI CACGTC 1 cut(s) 447
BmgT120I GGNCC 2 cut(s) 52, 546
BmiI GGNNCC 1 cut(s) 199
BmrFI CCNGG 3 cut(s) 364, 544, 549
BmsI GCATC 3 cut(s) 76, 174, 262
Bpu10I CCTNAGC 2 cut(s) 56, 368
BpuEI CTTGAG 1 cut(s) 532
BpuMI CCSGG 1 cut(s) 549
BsaJI CCNNGG 1 cut(s) 38
BsaWI WCCGGW 1 cut(s) 441
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 175, 176, 235, 369
Bse1I ACTGG 1 cut(s) 535
Bse3DI GCAATG 1 cut(s) 582
BseBI CCWGG 2 cut(s) 364, 544
BseDI CCNNGG 1 cut(s) 38
BseGI GGATG 2 cut(s) 91, 165
BseLI CCNNNNNNNGG 5 cut(s) 82, 175, 176, 235, 369
BseMI GCAATG 1 cut(s) 582
BseMII CTCAG 3 cut(s) 47, 59, 359
BseNI ACTGG 1 cut(s) 535
BseXI GCAGC 2 cut(s) 101, 511
BseYI CCCAGC 1 cut(s) 379
BshFI GGCC 3 cut(s) 43, 220, 547
BshNI GGYRCC 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 333
BsiHKCI CYCGRG 1 cut(s) 419
BsiSI CCGG 3 cut(s) 442, 503, 549
BsiWI CGTACG 1 cut(s) 324
BslFI GGGAC 1 cut(s) 192
BslI CCNNNNNNNGG 5 cut(s) 82, 175, 176, 235, 369
BsmAI GTCTC 1 cut(s) 416
BsmBI CGTCTC 1 cut(s) 416
BsmFI GGGAC 1 cut(s) 192
BsnI GGCC 3 cut(s) 43, 220, 547
BsoBI CYCGRG 1 cut(s) 419
Bsp1286I GDGCHC 1 cut(s) 333
Bsp143I GATC 3 cut(s) 155, 507, 619
BspACI CCGC 3 cut(s) 92, 229, 434
BspANI GGCC 3 cut(s) 43, 220, 547
BspCNI CTCAG 3 cut(s) 48, 58, 360
BspLI GGNNCC 1 cut(s) 199
BspPI GGATC 1 cut(s) 163
BspT107I GGYRCC 1 cut(s) 197
BsrDI GCAATG 1 cut(s) 582
BsrI ACTGG 1 cut(s) 535
BssECI CCNNGG 1 cut(s) 38
BssMI GATC 3 cut(s) 155, 507, 619
BssT1I CCWWGG 1 cut(s) 38
Bst2UI CCWGG 2 cut(s) 364, 544
Bst4CI ACNGT 2 cut(s) 294, 337
BstDEI CTNAG 5 cut(s) 45, 56, 151, 368, 645
BstEII GGTNACC 1 cut(s) 98
BstENI CCTNNNNNAGG 1 cut(s) 367
BstF5I GGATG 2 cut(s) 91, 165
BstKTI GATC 3 cut(s) 158, 510, 622
BstMAI GTCTC 1 cut(s) 416
BstMBI GATC 3 cut(s) 155, 507, 619
BstNI CCWGG 2 cut(s) 364, 544
BstPI GGTNACC 1 cut(s) 98
BstSCI CCNGG 3 cut(s) 362, 542, 547
BstV1I GCAGC 2 cut(s) 101, 511
BstX2I RGATCY 2 cut(s) 155, 507
BstYI RGATCY 2 cut(s) 155, 507
BsuI GTATCC 1 cut(s) 330
BsuRI GGCC 3 cut(s) 43, 220, 547
BtrI CACGTC 1 cut(s) 447
BtsCI GGATG 2 cut(s) 91, 165
BtsI GCAGTG 1 cut(s) 282
BtsIMutI CAGTG 3 cut(s) 282, 479, 649
Cfr13I GGNCC 2 cut(s) 52, 546
CseI GACGC 2 cut(s) 231, 505
Csp6I GTAC 2 cut(s) 325, 416
CviAII CATG 2 cut(s) 173, 589
CviQI GTAC 2 cut(s) 325, 416
DdeI CTNAG 5 cut(s) 45, 56, 151, 368, 645
DpnI GATC 3 cut(s) 157, 509, 621
DpnII GATC 3 cut(s) 155, 507, 619
EciI GGCGGA 1 cut(s) 449
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 1 cut(s) 38
Eco147I AGGCCT 2 cut(s) 43, 220
Eco24I GRGCYC 1 cut(s) 333
Eco47I GGWCC 1 cut(s) 52
Eco53kI GAGCTC 1 cut(s) 331
Eco88I CYCGRG 1 cut(s) 419
Eco91I GGTNACC 1 cut(s) 98
EcoICRI GAGCTC 1 cut(s) 331
EcoNI CCTNNNNNAGG 1 cut(s) 367
EcoO65I GGTNACC 1 cut(s) 98
EcoRII CCWGG 2 cut(s) 362, 542
EcoT14I CCWWGG 1 cut(s) 38
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 38
Esp3I CGTCTC 1 cut(s) 416
FaeI CATG 2 cut(s) 176, 592
FaiI YATR 9 cut(s) 129, 174, 192, 251, 297, 413, 489, 525, 590
FaqI GGGAC 1 cut(s) 192
FatI CATG 2 cut(s) 172, 588
Fnu4HI GCNGC 2 cut(s) 90, 500
FokI GGATG 2 cut(s) 98, 152
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 2 cut(s) 90, 500
GluI GCNGC 2 cut(s) 90, 500
GsaI CCCAGC 1 cut(s) 383
HaeIII GGCC 3 cut(s) 43, 220, 547
HapII CCGG 3 cut(s) 442, 503, 549
HgaI GACGC 2 cut(s) 231, 505
Hin1II CATG 2 cut(s) 176, 592
HinfI GANTC 4 cut(s) 280, 408, 476, 539
HpaII CCGG 3 cut(s) 442, 503, 549
Hpy188I TCNGA 1 cut(s) 624
Hpy188III TCNNGA 1 cut(s) 511
HpyAV CCTTC 2 cut(s) 307, 398
HpyCH4III ACNGT 2 cut(s) 294, 337
HpyCH4IV ACGT 4 cut(s) 425, 446, 456, 485
HpyCH4V TGCA 8 cut(s) 89, 131, 165, 275, 491, 527, 572, 617
HpyF3I CTNAG 5 cut(s) 45, 56, 151, 368, 645
HpySE526I ACGT 4 cut(s) 425, 446, 456, 485
Hsp92II CATG 2 cut(s) 176, 592
Kzo9I GATC 3 cut(s) 155, 507, 619
Lsp1109I GCAGC 2 cut(s) 101, 511
LweI GCATC 3 cut(s) 76, 174, 262
MaeII ACGT 4 cut(s) 425, 446, 456, 485
MaeIII GTNAC 5 cut(s) 98, 243, 288, 452, 477
MalI GATC 3 cut(s) 157, 509, 621
MboI GATC 3 cut(s) 155, 507, 619
MflI RGATCY 2 cut(s) 155, 507
MhlI GDGCHC 1 cut(s) 333
MluCI AATT 4 cut(s) 15, 67, 142, 628
MlyI GAGTC 2 cut(s) 485, 548
MmeI TCCRAC 1 cut(s) 30
MseI TTAA 2 cut(s) 120, 600
MslI CAYNNNNRTG 1 cut(s) 587
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 3 cut(s) 442, 503, 549
MspR9I CCNGG 3 cut(s) 364, 544, 549
MvaI CCWGG 2 cut(s) 364, 544
NciI CCSGG 1 cut(s) 549
NdeII GATC 3 cut(s) 155, 507, 619
NlaIII CATG 2 cut(s) 176, 592
NlaIV GGNNCC 1 cut(s) 199
NmuCI GTSAC 2 cut(s) 243, 477
PceI AGGCCT 2 cut(s) 43, 220
PfeI GAWTC 2 cut(s) 280, 408
Pfl23II CGTACG 1 cut(s) 324
PkrI GCNGC 2 cut(s) 91, 501
PleI GAGTC 2 cut(s) 484, 547
PpsI GAGTC 2 cut(s) 484, 547
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 2 cut(s) 362, 542
PspEI GGTNACC 1 cut(s) 98
PspFI CCCAGC 1 cut(s) 379
PspGI CCWGG 2 cut(s) 362, 542
PspLI CGTACG 1 cut(s) 324
PspN4I GGNNCC 1 cut(s) 199
PspPI GGNCC 2 cut(s) 52, 546
PsuI RGATCY 2 cut(s) 155, 507
RsaI GTAC 2 cut(s) 326, 417
RsaNI GTAC 2 cut(s) 325, 416
RseI CAYNNNNRTG 1 cut(s) 587
SacI GAGCTC 1 cut(s) 333
SaqAI TTAA 2 cut(s) 120, 600
SatI GCNGC 2 cut(s) 90, 500
Sau3AI GATC 3 cut(s) 155, 507, 619
Sau96I GGNCC 2 cut(s) 52, 546
SchI GAGTC 2 cut(s) 485, 548
ScrFI CCNGG 3 cut(s) 364, 544, 549
SduI GDGCHC 1 cut(s) 333
SfaNI GCATC 3 cut(s) 76, 174, 262
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 587
SmlI CTYRAG 1 cut(s) 511
SmoI CTYRAG 1 cut(s) 511
Sse9I AATT 4 cut(s) 15, 67, 142, 628
SseBI AGGCCT 2 cut(s) 43, 220
SsiI CCGC 3 cut(s) 92, 229, 434
SspI AATATT 1 cut(s) 610
SstI GAGCTC 1 cut(s) 333
StuI AGGCCT 2 cut(s) 43, 220
StyD4I CCNGG 3 cut(s) 362, 542, 547
StyI CCWWGG 1 cut(s) 38
TaaI ACNGT 2 cut(s) 294, 337
TaiI ACGT 4 cut(s) 428, 449, 459, 488
TasI AATT 4 cut(s) 15, 67, 142, 628
TfiI GAWTC 2 cut(s) 280, 408
Tru1I TTAA 2 cut(s) 120, 600
Tru9I TTAA 2 cut(s) 120, 600
TscAI CASTG 2 cut(s) 282, 486
TseFI GTSAC 2 cut(s) 243, 477
TseI GCWGC 2 cut(s) 89, 499
Tsp45I GTSAC 2 cut(s) 243, 477
TspDTI ATGAA 2 cut(s) 17, 417
TspGWI ACGGA 1 cut(s) 312
TspRI CASTG 2 cut(s) 282, 486
VpaK11BI GGWCC 1 cut(s) 52
XagI CCTNNNNNAGG 1 cut(s) 367
XapI RAATTY 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.