pycom05g15470

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001853
Physical Location & Seq
Forward (+)
19044947 .. 19046378
1432 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g15470.1

Sequence Viewer

Length: 822 bp
ATGTCAGAAAATGGTTCAACAGACGTTGTAGGGTCGTCTTCGACCCATCCGGAGGAATCTTCACAAGAAATTGAAGGGAGCACTACAATTGTAAGCTTTGAAGGCTACACTTTTCCAACGAACAAGGGGAGAGGTCGCCATCCATTACCACCTGGCCTCGGAACACCAGTTGTAGCAATCGTACGTAGTCCGAATCTGTCAATTTATAGCCCTCCACGACCTTATAGACTTCCACTGTCCCCAGATCACAGACCAGGGGATGAAAATGCTTCAGGATTTACACATATATTTGATCAAGGAAGAAAAGAAAGGCGTGATGATGCTAAAGAAACAGAAACGTTGCCAATTAACGCCAACCTAGTTCCCTATGTCCCACTACATCTTGCTGCTCTCAATGGTGACTGGATTGCTGCAAACAATTTCTTGCTATCAAATCCTGAAGCTGCGAGGGCTAAGATCACAAAGGGTTCGGAAACTGCTCTCCACATTGCTGCCGGGGCCAAACATACATCGTTTGTTGAGGAGCTAGTTAAATGGATGATACCGCCTGACCTAGAACTGAAGAATGATGTTGGAAACACTGCCCTCTTTTTTGCTGCTGTTTCCGGTGTCAAAAGGATCGCCGAGATAATGGTGGATAAGAATCCGAAGTTACCCCAAATCAGATGTAGTAAAGATTCAACACCTCTTCATATGGCTACTTTGTTTGGGCACAGGGAGATGGTATGGTATCTTTACGACAAGACTGATACAATTTTGAAGGACTCCGACCGTGTGGGGCTTCTTATTGCCGCAATAACAGCAGATCTTTATGGTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

274

Amino Acids

29.88

Weight (kDa)

6.14

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 175 - 250 3.5e-11 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 49
AciI CCGC 2 cut(s) 545, 792
AclI AACGTT 1 cut(s) 338
AclWI GGATC 1 cut(s) 626
AcuI CTGAAG 3 cut(s) 255, 459, 581
AfaI GTAC 1 cut(s) 183
AfiI CCNNNNNNNGG 2 cut(s) 52, 158
AgsI TTSAA 5 cut(s) 18, 74, 101, 681, 760
AjnI CCWGG 2 cut(s) 151, 253
AluBI AGCT 3 cut(s) 96, 443, 526
AluI AGCT 3 cut(s) 96, 443, 526
Alw21I GWGCWC 1 cut(s) 83
AlwI GGATC 1 cut(s) 626
Aor13HI TCCGGA 1 cut(s) 49
AoxI GGCC 2 cut(s) 154, 498
ApeKI GCWGC 5 cut(s) 386, 410, 443, 491, 596
AspS9I GGNCC 1 cut(s) 498
AsuC2I CCSGG 1 cut(s) 496
AsuHPI GGTGA 1 cut(s) 410
BaeGI GKGCMC 1 cut(s) 714
BbsI GAAGAC 1 cut(s) 30
Bbv12I GWGCWC 1 cut(s) 83
BbvI GCAGC 5 cut(s) 373, 397, 430, 478, 583
BccI CCATC 3 cut(s) 54, 147, 715
BciT130I CCWGG 2 cut(s) 153, 255
BclI TGATCA 1 cut(s) 292
BcnI CCSGG 1 cut(s) 496
BfaI CTAG 3 cut(s) 359, 527, 554
BglII AGATCT 1 cut(s) 805
BisI GCNGC 6 cut(s) 387, 411, 444, 492, 597, 792
BlsI GCNGC 6 cut(s) 388, 412, 445, 493, 598, 793
Bme1390I CCNGG 3 cut(s) 153, 255, 496
BmgT120I GGNCC 1 cut(s) 498
BmiI GGNNCC 1 cut(s) 499
BmrFI CCNGG 3 cut(s) 153, 255, 496
BmsI GCATC 1 cut(s) 310
BpiI GAAGAC 1 cut(s) 30
BpuMI CCSGG 1 cut(s) 496
BsaAI YACGTR 1 cut(s) 185
BsaBI GATNNNNATC 1 cut(s) 642
BsaJI CCNNGG 3 cut(s) 157, 254, 495
BsaWI WCCGGW 2 cut(s) 49, 605
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 158
Bse1I ACTGG 2 cut(s) 167, 407
Bse3DI GCAATG 1 cut(s) 486
Bse8I GATNNNNATC 1 cut(s) 642
BseAI TCCGGA 1 cut(s) 49
BseBI CCWGG 2 cut(s) 153, 255
BseDI CCNNGG 3 cut(s) 157, 254, 495
BseGI GGATG 4 cut(s) 46, 139, 265, 543
BseJI GATNNNNATC 1 cut(s) 642
BseLI CCNNNNNNNGG 2 cut(s) 52, 158
BseMI GCAATG 1 cut(s) 486
BseNI ACTGG 2 cut(s) 167, 407
BseRI GAGGAG 1 cut(s) 536
BseSI GKGCMC 1 cut(s) 714
BseXI GCAGC 5 cut(s) 373, 397, 430, 478, 583
Bsh1285I CGRYCG 1 cut(s) 772
BshFI GGCC 2 cut(s) 156, 500
BsiEI CGRYCG 1 cut(s) 772
BsiHKAI GWGCWC 1 cut(s) 83
BsiSI CCGG 3 cut(s) 50, 495, 606
BsiWI CGTACG 1 cut(s) 181
BslFI GGGAC 2 cut(s) 223, 356
BslI CCNNNNNNNGG 2 cut(s) 52, 158
BsmFI GGGAC 2 cut(s) 223, 356
BsnI GGCC 2 cut(s) 156, 500
Bsp1286I GDGCHC 2 cut(s) 83, 714
Bsp13I TCCGGA 1 cut(s) 49
Bsp143I GATC 5 cut(s) 244, 292, 456, 618, 805
BspACI CCGC 2 cut(s) 545, 792
BspANI GGCC 2 cut(s) 156, 500
BspEI TCCGGA 1 cut(s) 49
BspLI GGNNCC 1 cut(s) 499
BspPI GGATC 1 cut(s) 626
BsrDI GCAATG 1 cut(s) 486
BsrI ACTGG 2 cut(s) 167, 407
BssECI CCNNGG 3 cut(s) 157, 254, 495
BssMI GATC 5 cut(s) 244, 292, 456, 618, 805
Bst2UI CCWGG 2 cut(s) 153, 255
Bst4CI ACNGT 2 cut(s) 237, 773
Bst6I CTCTTC 1 cut(s) 693
BstBAI YACGTR 1 cut(s) 185
BstDEI CTNAG 1 cut(s) 453
BstF5I GGATG 4 cut(s) 46, 139, 265, 543
BstKTI GATC 5 cut(s) 247, 295, 459, 621, 808
BstMBI GATC 5 cut(s) 244, 292, 456, 618, 805
BstMCI CGRYCG 1 cut(s) 772
BstMWI GCNNNNNNNGC 4 cut(s) 102, 449, 497, 800
BstNI CCWGG 2 cut(s) 153, 255
BstSCI CCNGG 3 cut(s) 151, 253, 494
BstSLI GKGCMC 1 cut(s) 714
BstSNI TACGTA 1 cut(s) 185
BstV1I GCAGC 5 cut(s) 373, 397, 430, 478, 583
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 1 cut(s) 805
BstYI RGATCY 1 cut(s) 805
BsuRI GGCC 2 cut(s) 156, 500
BtsCI GGATG 4 cut(s) 46, 139, 265, 543
BtsI GCAGTG 1 cut(s) 579
BtsIMutI CAGTG 2 cut(s) 233, 579
Cfr13I GGNCC 1 cut(s) 498
Csp6I GTAC 1 cut(s) 182
CviQI GTAC 1 cut(s) 182
DdeI CTNAG 1 cut(s) 453
DpnI GATC 5 cut(s) 246, 294, 458, 620, 807
DpnII GATC 5 cut(s) 244, 292, 456, 618, 805
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
Eco105I TACGTA 1 cut(s) 185
Eco57I CTGAAG 3 cut(s) 255, 459, 581
EcoRII CCWGG 2 cut(s) 151, 253
FaqI GGGAC 2 cut(s) 223, 356
FauNDI CATATG 1 cut(s) 693
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 6 cut(s) 387, 411, 444, 492, 597, 792
FokI GGATG 4 cut(s) 33, 126, 272, 550
Fsp4HI GCNGC 6 cut(s) 387, 411, 444, 492, 597, 792
FspBI CTAG 3 cut(s) 359, 527, 554
GluI GCNGC 6 cut(s) 387, 411, 444, 492, 597, 792
HaeIII GGCC 2 cut(s) 156, 500
HapII CCGG 3 cut(s) 50, 495, 606
HindIII AAGCTT 1 cut(s) 94
HinfI GANTC 5 cut(s) 56, 193, 643, 677, 764
HpaII CCGG 3 cut(s) 50, 495, 606
HphI GGTGA 1 cut(s) 410
Hpy188I TCNGA 7 cut(s) 7, 161, 192, 472, 648, 665, 769
Hpy188III TCNNGA 3 cut(s) 50, 273, 437
HpyAV CCTTC 3 cut(s) 68, 95, 754
HpyCH4III ACNGT 2 cut(s) 237, 773
HpyCH4IV ACGT 3 cut(s) 24, 184, 338
HpyCH4V TGCA 1 cut(s) 413
HpyF10VI GCNNNNNNNGC 4 cut(s) 102, 449, 497, 800
HpyF3I CTNAG 1 cut(s) 453
HpySE526I ACGT 3 cut(s) 24, 184, 338
Kpn2I TCCGGA 1 cut(s) 49
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 5 cut(s) 244, 292, 456, 618, 805
LmnI GCTCC 2 cut(s) 78, 523
Lsp1109I GCAGC 5 cut(s) 373, 397, 430, 478, 583
LweI GCATC 1 cut(s) 310
MaeI CTAG 3 cut(s) 359, 527, 554
MaeII ACGT 3 cut(s) 24, 184, 338
MaeIII GTNAC 2 cut(s) 398, 651
MalI GATC 5 cut(s) 246, 294, 458, 620, 807
MboI GATC 5 cut(s) 244, 292, 456, 618, 805
MboII GAAGA 5 cut(s) 30, 51, 312, 574, 680
MfeI CAATTG 1 cut(s) 87
MflI RGATCY 1 cut(s) 805
MhlI GDGCHC 2 cut(s) 83, 714
MluCI AATT 6 cut(s) 69, 87, 201, 345, 418, 753
MlyI GAGTC 1 cut(s) 758
MmeI TCCRAC 3 cut(s) 140, 553, 792
MnlI CCTC 8 cut(s) 46, 125, 167, 222, 441, 514, 596, 696
MroI TCCGGA 1 cut(s) 49
MseI TTAA 2 cut(s) 348, 531
MspI CCGG 3 cut(s) 50, 495, 606
MspR9I CCNGG 3 cut(s) 153, 255, 496
MunI CAATTG 1 cut(s) 87
MvaI CCWGG 2 cut(s) 153, 255
MwoI GCNNNNNNNGC 4 cut(s) 102, 449, 497, 800
NciI CCSGG 1 cut(s) 496
NdeI CATATG 1 cut(s) 693
NdeII GATC 5 cut(s) 244, 292, 456, 618, 805
NlaIV GGNNCC 1 cut(s) 499
NmeAIII GCCGAG 1 cut(s) 649
NmuCI GTSAC 1 cut(s) 398
PfeI GAWTC 4 cut(s) 56, 193, 643, 677
Pfl23II CGTACG 1 cut(s) 181
PkrI GCNGC 6 cut(s) 388, 412, 445, 493, 598, 793
PleI GAGTC 1 cut(s) 758
PpsI GAGTC 1 cut(s) 758
Ppu21I YACGTR 1 cut(s) 185
Psp1406I AACGTT 1 cut(s) 338
Psp6I CCWGG 2 cut(s) 151, 253
PspGI CCWGG 2 cut(s) 151, 253
PspLI CGTACG 1 cut(s) 181
PspN4I GGNNCC 1 cut(s) 499
PspPI GGNCC 1 cut(s) 498
PsuI RGATCY 1 cut(s) 805
RsaI GTAC 1 cut(s) 183
RsaNI GTAC 1 cut(s) 182
SaqAI TTAA 2 cut(s) 348, 531
SatI GCNGC 6 cut(s) 387, 411, 444, 492, 597, 792
Sau3AI GATC 5 cut(s) 244, 292, 456, 618, 805
Sau96I GGNCC 1 cut(s) 498
SchI GAGTC 1 cut(s) 758
ScrFI CCNGG 3 cut(s) 153, 255, 496
SduI GDGCHC 2 cut(s) 83, 714
SfaNI GCATC 1 cut(s) 310
SnaBI TACGTA 1 cut(s) 185
Sse9I AATT 6 cut(s) 69, 87, 201, 345, 418, 753
SsiI CCGC 2 cut(s) 545, 792
SspMI CTAG 3 cut(s) 359, 527, 554
StyD4I CCNGG 3 cut(s) 151, 253, 494
TaaI ACNGT 2 cut(s) 237, 773
TaiI ACGT 3 cut(s) 27, 187, 341
TaqI TCGA 1 cut(s) 41
TasI AATT 6 cut(s) 69, 87, 201, 345, 418, 753
TauI GCSGC 1 cut(s) 794
TfiI GAWTC 4 cut(s) 56, 193, 643, 677
Tru1I TTAA 2 cut(s) 348, 531
Tru9I TTAA 2 cut(s) 348, 531
TscAI CASTG 2 cut(s) 240, 586
TseFI GTSAC 1 cut(s) 398
TseI GCWGC 5 cut(s) 386, 410, 443, 491, 596
Tsp45I GTSAC 1 cut(s) 398
TspDTI ATGAA 2 cut(s) 276, 680
TspRI CASTG 2 cut(s) 240, 586
XspI CTAG 3 cut(s) 359, 527, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.