Rroxscaffold_2G00131200

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001853
Physical Location & Seq
Reverse (-)
67739790 .. 67741571
1782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00131200.1

Sequence Viewer

Length: 1032 bp
ATGTCAGAGAAACTAACCACATCATCGCCATTTGAAGTTGAAGAAGGATTCTCAACCCAAGAGAACTTCCCAAAAGAATCTGCAGGAGAAGTAATACAAGAGACATGTGGACTAACCTTTGGAGGCGACAATGGCACCGCTGCCACATCCGGCAGACGGTCATTTGCAACAAGCGATGGAGGGTACAGTGCTGGTCCTCCGTCTAGCGGGACGACATGTGCAACAATAAGCTACGGAGGTTACTACAGCAGCGGTGCAACGTCTAGCTGCAGACCGACGTGTCATCCGTTACCACCTGGCCTCGGAACACCAATTATCGCAATTGTTCATAGTCCTGAATTTACGTTTGGTGGATGGGGTAATTCTACTAAAAATGTCGATGTAGAGATTGGCCATGATCAGCAACCTGATGATCTGGAGGCTACATATACTGCAGAAGAAGCCGAGAGAAAACAAAGGGAAGATGCAGCTTCCCCAGTAATAACCCACTCAGGCTCCATTTCAACGGTGATAAATAGCGACCTAGTACCCCATGTTCCTCTACATCTTGCTGCACTCAAAGGTGACTGGAATACTGCTAAAATCATCCTGCAATCAAAGCCTGGAGCTGTGAGGGCTAGGATCACAAAGGGTTCTGAAACAGCTCTTCACATCGCAGCCGGAGCTAAACATACGTACTTTGTTGAAGAGCTAGTTAAATGGATGACTGCAGAGGATCTTGAACTGAAGAATGAAGTGGAAAACACTGCCCTATTTTTTGCTGCTGTTTCTGGTATCAAAAGGATCGCTGAGGTAATGGTGGAGGTTAATCCGAGATTACCTCGGATCCGAGGTAGTAACAATTCAACAGCTCTGCATATGGCTACTTTGCTAGGGCACAGGGAGATGGCACGGTATCTTTACAGCAAGACTAAAAGAATTTTGACCTACCAGGACCGTGTGGGGCTTCTTATTGCTGCCATAACAGCAGATCTCTATGGTAAGTTTACTAGCACTATTGAAATTTTATCCTCAGTTCCCACTTTTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

343

Amino Acids

36.79

Weight (kDa)

5.72

Isoelectric Point (pI)

43.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 230 - 304 3.2e-08 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 279
AccB1I GGYRCC 1 cut(s) 134
AciI CCGC 3 cut(s) 138, 207, 252
AclWI GGATC 5 cut(s) 629, 723, 791, 820, 833
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 3 cut(s) 338, 918, 1002
AcuI CTGAAG 1 cut(s) 746
AfaI GTAC 3 cut(s) 185, 528, 677
AfiI CCNNNNNNNGG 3 cut(s) 156, 206, 302
AflIII ACRYGT 3 cut(s) 104, 215, 278
AgsI TTSAA 7 cut(s) 35, 41, 504, 686, 722, 846, 1001
AjiI CACGTC 1 cut(s) 279
AjnI CCWGG 3 cut(s) 295, 601, 930
AjuI GAANNNNNNNTTGG 2 cut(s) 330, 362
AluBI AGCT 8 cut(s) 231, 267, 470, 608, 644, 665, 691, 851
AluI AGCT 8 cut(s) 231, 267, 470, 608, 644, 665, 691, 851
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 5 cut(s) 629, 723, 791, 820, 833
AoxI GGCC 2 cut(s) 298, 391
ApeKI GCWGC 8 cut(s) 140, 249, 267, 467, 551, 656, 761, 956
ApoI RAATTY 3 cut(s) 338, 918, 1002
AspS9I GGNCC 2 cut(s) 194, 934
AsuHPI GGTGA 2 cut(s) 520, 575
AvaII GGWCC 2 cut(s) 194, 934
BaeGI GKGCMC 1 cut(s) 879
BalI TGGCCA 1 cut(s) 393
BamHI GGATCC 1 cut(s) 825
BanI GGYRCC 1 cut(s) 134
BbvCI CCTCAGC 1 cut(s) 789
BbvI GCAGC 8 cut(s) 127, 254, 261, 479, 538, 668, 748, 943
BccI CCATC 3 cut(s) 170, 348, 880
BciT130I CCWGG 3 cut(s) 297, 603, 932
BclI TGATCA 1 cut(s) 397
BcoDI GTCTC 1 cut(s) 95
BfaI CTAG 8 cut(s) 204, 264, 524, 618, 692, 872, 990, 1030
BfmI CTRYAG 5 cut(s) 81, 244, 268, 432, 708
BglII AGATCT 1 cut(s) 970
BisI GCNGC 8 cut(s) 141, 250, 268, 468, 552, 657, 762, 957
BlsI GCNGC 8 cut(s) 142, 251, 269, 469, 553, 658, 763, 958
Bme1390I CCNGG 3 cut(s) 297, 603, 932
Bme18I GGWCC 2 cut(s) 194, 934
BmgBI CACGTC 1 cut(s) 279
BmgT120I GGNCC 2 cut(s) 194, 934
BmiI GGNNCC 3 cut(s) 136, 496, 827
BmrFI CCNGG 3 cut(s) 297, 603, 932
BmrI ACTGGG 1 cut(s) 470
BmsI GCATC 1 cut(s) 454
BmuI ACTGGG 1 cut(s) 470
BplI GAGNNNNNCTC 2 cut(s) 805, 837
BpmI CTGGAG 2 cut(s) 437, 624
Bpu10I CCTNAGC 1 cut(s) 789
BsaAI YACGTR 1 cut(s) 675
BsaJI CCNNGG 3 cut(s) 301, 821, 829
BsaXI ACNNNNNCTCC 2 cut(s) 479, 509
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 206, 302
Bse1I ACTGG 2 cut(s) 476, 572
BseBI CCWGG 3 cut(s) 297, 603, 932
BseDI CCNNGG 3 cut(s) 301, 821, 829
BseGI GGATG 5 cut(s) 146, 283, 359, 585, 708
BseLI CCNNNNNNNGG 3 cut(s) 156, 206, 302
BseMII CTCAG 3 cut(s) 504, 780, 1026
BseNI ACTGG 2 cut(s) 476, 572
BseSI GKGCMC 1 cut(s) 879
BseXI GCAGC 8 cut(s) 127, 254, 261, 479, 538, 668, 748, 943
BsgI GTGCAG 1 cut(s) 537
BshFI GGCC 2 cut(s) 300, 393
BshNI GGYRCC 1 cut(s) 134
BsiSI CCGG 2 cut(s) 150, 660
BslFI GGGAC 1 cut(s) 223
BslI CCNNNNNNNGG 3 cut(s) 156, 206, 302
BsmAI GTCTC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 223
BsnI GGCC 2 cut(s) 300, 393
Bsp1286I GDGCHC 1 cut(s) 879
Bsp143I GATC 7 cut(s) 397, 412, 621, 715, 783, 825, 970
BspACI CCGC 3 cut(s) 138, 207, 252
BspANI GGCC 2 cut(s) 300, 393
BspCNI CTCAG 3 cut(s) 503, 781, 1025
BspLI GGNNCC 3 cut(s) 136, 496, 827
BspMAI CTGCAG 4 cut(s) 85, 272, 436, 712
BspPI GGATC 5 cut(s) 629, 723, 791, 820, 833
BspQI GCTCTTC 2 cut(s) 651, 681
BspT107I GGYRCC 1 cut(s) 134
BsrI ACTGG 2 cut(s) 476, 572
BssECI CCNNGG 3 cut(s) 301, 821, 829
BssMI GATC 7 cut(s) 397, 412, 621, 715, 783, 825, 970
Bst2UI CCWGG 3 cut(s) 297, 603, 932
Bst4CI ACNGT 5 cut(s) 159, 188, 508, 894, 938
Bst6I CTCTTC 2 cut(s) 651, 681
BstBAI YACGTR 1 cut(s) 675
BstDEI CTNAG 3 cut(s) 490, 789, 1012
BstF5I GGATG 5 cut(s) 146, 283, 359, 585, 708
BstKTI GATC 7 cut(s) 400, 415, 624, 718, 786, 828, 973
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 7 cut(s) 397, 412, 621, 715, 783, 825, 970
BstMWI GCNNNNNNNGC 6 cut(s) 132, 440, 598, 614, 662, 965
BstNI CCWGG 3 cut(s) 297, 603, 932
BstNSI RCATGY 2 cut(s) 108, 219
BstSCI CCNGG 3 cut(s) 295, 601, 930
BstSFI CTRYAG 5 cut(s) 81, 244, 268, 432, 708
BstSLI GKGCMC 1 cut(s) 879
BstSNI TACGTA 1 cut(s) 675
BstV1I GCAGC 8 cut(s) 127, 254, 261, 479, 538, 668, 748, 943
BstX2I RGATCY 3 cut(s) 715, 825, 970
BstYI RGATCY 3 cut(s) 715, 825, 970
BsuRI GGCC 2 cut(s) 300, 393
BtgZI GCGATG 3 cut(s) 9, 189, 637
BtrI CACGTC 1 cut(s) 279
BtsCI GGATG 5 cut(s) 146, 283, 359, 585, 708
BtsI GCAGTG 1 cut(s) 744
BtsIMutI CAGTG 2 cut(s) 193, 744
Cfr13I GGNCC 2 cut(s) 194, 934
Csp6I GTAC 3 cut(s) 184, 527, 676
CviAII CATG 4 cut(s) 105, 216, 395, 533
CviQI GTAC 3 cut(s) 184, 527, 676
DdeI CTNAG 3 cut(s) 490, 789, 1012
DpnI GATC 7 cut(s) 399, 414, 623, 717, 785, 827, 972
DpnII GATC 7 cut(s) 397, 412, 621, 715, 783, 825, 970
DrdI GACNNNNNNGTC 1 cut(s) 279
DseDI GACNNNNNNGTC 1 cut(s) 279
EaeI YGGCCR 1 cut(s) 391
Eam1104I CTCTTC 2 cut(s) 651, 681
EarI CTCTTC 2 cut(s) 651, 681
Eco105I TACGTA 1 cut(s) 675
Eco47I GGWCC 2 cut(s) 194, 934
Eco57I CTGAAG 1 cut(s) 746
EcoRII CCWGG 3 cut(s) 295, 601, 930
FaeI CATG 4 cut(s) 108, 219, 398, 536
FaqI GGGAC 1 cut(s) 223
FatI CATG 4 cut(s) 104, 215, 394, 532
FauI CCCGC 1 cut(s) 200
FauNDI CATATG 1 cut(s) 858
FbaI TGATCA 1 cut(s) 397
Fnu4HI GCNGC 8 cut(s) 141, 250, 268, 468, 552, 657, 762, 957
FokI GGATG 5 cut(s) 133, 270, 366, 572, 715
Fsp4HI GCNGC 8 cut(s) 141, 250, 268, 468, 552, 657, 762, 957
FspBI CTAG 8 cut(s) 204, 264, 524, 618, 692, 872, 990, 1030
GluI GCNGC 8 cut(s) 141, 250, 268, 468, 552, 657, 762, 957
GsuI CTGGAG 2 cut(s) 437, 624
HaeIII GGCC 2 cut(s) 300, 393
HapII CCGG 2 cut(s) 150, 660
Hin1II CATG 4 cut(s) 108, 219, 398, 536
HinfI GANTC 2 cut(s) 48, 77
HpaII CCGG 2 cut(s) 150, 660
HphI GGTGA 2 cut(s) 520, 575
Hpy166II GTNNAC 2 cut(s) 110, 987
Hpy188I TCNGA 6 cut(s) 7, 305, 637, 813, 825, 830
Hpy188III TCNNGA 3 cut(s) 335, 416, 719
Hpy8I GTNNAC 2 cut(s) 110, 987
Hpy99I CGWCG 1 cut(s) 280
HpyAV CCTTC 1 cut(s) 38
HpyCH4III ACNGT 5 cut(s) 159, 188, 508, 894, 938
HpyCH4IV ACGT 4 cut(s) 260, 278, 344, 674
HpyF10VI GCNNNNNNNGC 6 cut(s) 132, 440, 598, 614, 662, 965
HpyF3I CTNAG 3 cut(s) 490, 789, 1012
HpySE526I ACGT 4 cut(s) 260, 278, 344, 674
Hsp92II CATG 4 cut(s) 108, 219, 398, 536
Ksp22I TGATCA 1 cut(s) 397
Kzo9I GATC 7 cut(s) 397, 412, 621, 715, 783, 825, 970
LguI GCTCTTC 2 cut(s) 651, 681
LmnI GCTCC 3 cut(s) 500, 605, 662
Lsp1109I GCAGC 8 cut(s) 127, 254, 261, 479, 538, 668, 748, 943
LweI GCATC 1 cut(s) 454
MaeI CTAG 8 cut(s) 204, 264, 524, 618, 692, 872, 990, 1030
MaeII ACGT 4 cut(s) 260, 278, 344, 674
MaeIII GTNAC 4 cut(s) 239, 288, 563, 836
MalI GATC 7 cut(s) 399, 414, 623, 717, 785, 827, 972
MboI GATC 7 cut(s) 397, 412, 621, 715, 783, 825, 970
MboII GAAGA 6 cut(s) 53, 449, 473, 638, 698, 739
MfeI CAATTG 1 cut(s) 321
MflI RGATCY 3 cut(s) 715, 825, 970
MhlI GDGCHC 1 cut(s) 879
MlsI TGGCCA 1 cut(s) 393
MluCI AATT 7 cut(s) 312, 321, 338, 361, 841, 918, 1002
MluNI TGGCCA 1 cut(s) 393
Mox20I TGGCCA 1 cut(s) 393
MscI TGGCCA 1 cut(s) 393
MseI TTAA 2 cut(s) 696, 807
Msp20I TGGCCA 1 cut(s) 393
MspA1I CMGCKG 2 cut(s) 140, 252
MspI CCGG 2 cut(s) 150, 660
MspR9I CCNGG 3 cut(s) 297, 603, 932
MunI CAATTG 1 cut(s) 321
MvaI CCWGG 3 cut(s) 297, 603, 932
MwoI GCNNNNNNNGC 6 cut(s) 132, 440, 598, 614, 662, 965
NdeI CATATG 1 cut(s) 858
NdeII GATC 7 cut(s) 397, 412, 621, 715, 783, 825, 970
NlaIII CATG 4 cut(s) 108, 219, 398, 536
NlaIV GGNNCC 3 cut(s) 136, 496, 827
NmeAIII GCCGAG 1 cut(s) 469
NmuCI GTSAC 1 cut(s) 563
NspI RCATGY 2 cut(s) 108, 219
PciI ACATGT 2 cut(s) 104, 215
PciSI GCTCTTC 2 cut(s) 651, 681
PcsI WCGNNNNNNNCGW 1 cut(s) 284
PfeI GAWTC 2 cut(s) 48, 77
PkrI GCNGC 8 cut(s) 142, 251, 269, 469, 553, 658, 763, 958
Ppu21I YACGTR 1 cut(s) 675
PscI ACATGT 2 cut(s) 104, 215
Psp6I CCWGG 3 cut(s) 295, 601, 930
PspGI CCWGG 3 cut(s) 295, 601, 930
PspN4I GGNNCC 3 cut(s) 136, 496, 827
PspPI GGNCC 2 cut(s) 194, 934
PsrI GAACNNNNNNTAC 2 cut(s) 519, 551
PstI CTGCAG 4 cut(s) 85, 272, 436, 712
PsuI RGATCY 3 cut(s) 715, 825, 970
RsaI GTAC 3 cut(s) 185, 528, 677
RsaNI GTAC 3 cut(s) 184, 527, 676
SapI GCTCTTC 2 cut(s) 651, 681
SaqAI TTAA 2 cut(s) 696, 807
SatI GCNGC 8 cut(s) 141, 250, 268, 468, 552, 657, 762, 957
Sau3AI GATC 7 cut(s) 397, 412, 621, 715, 783, 825, 970
Sau96I GGNCC 2 cut(s) 194, 934
ScrFI CCNGG 3 cut(s) 297, 603, 932
SduI GDGCHC 1 cut(s) 879
SfaNI GCATC 1 cut(s) 454
SfcI CTRYAG 5 cut(s) 81, 244, 268, 432, 708
SinI GGWCC 2 cut(s) 194, 934
SnaBI TACGTA 1 cut(s) 675
Sse9I AATT 7 cut(s) 312, 321, 338, 361, 841, 918, 1002
SsiI CCGC 3 cut(s) 138, 207, 252
SspMI CTAG 8 cut(s) 204, 264, 524, 618, 692, 872, 990, 1030
StyD4I CCNGG 3 cut(s) 295, 601, 930
TaaI ACNGT 5 cut(s) 159, 188, 508, 894, 938
TaiI ACGT 4 cut(s) 263, 281, 347, 677
TaqI TCGA 1 cut(s) 378
TaqII GACCGA 1 cut(s) 289
TasI AATT 7 cut(s) 312, 321, 338, 361, 841, 918, 1002
TfiI GAWTC 2 cut(s) 48, 77
Tru1I TTAA 2 cut(s) 696, 807
Tru9I TTAA 2 cut(s) 696, 807
TscAI CASTG 2 cut(s) 193, 751
TseFI GTSAC 1 cut(s) 563
TseI GCWGC 8 cut(s) 140, 249, 267, 467, 551, 656, 761, 956
Tsp45I GTSAC 1 cut(s) 563
TspDTI ATGAA 2 cut(s) 317, 747
TspGWI ACGGA 3 cut(s) 189, 249, 276
TspRI CASTG 2 cut(s) 193, 751
VpaK11BI GGWCC 2 cut(s) 194, 934
XapI RAATTY 3 cut(s) 338, 918, 1002
XceI RCATGY 2 cut(s) 108, 219
XspI CTAG 8 cut(s) 204, 264, 524, 618, 692, 872, 990, 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.