Rorug04G0016000

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001853
Physical Location & Seq
Forward (+)
2490044 .. 2491626
1583 bp
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UTR
Exon/CDS
Intron
Rorug04G0016000.1

Sequence Viewer

Length: 411 bp
ATGGATAGCAGGCAAGCAGTTGAATTCGTAATTTCGAACGTGATTTTGTGCAATGACCGCTCAAGGAATCAATGCCAGAGACATCAAGAAGCTTCAGGACTCAGGGATCTACACCTGCAATGGCTTGATGATGCACACCAAAAAGAGAAAAATCTTGCGCGCATTACAACCGGAAGCCAAGCCCTTGTTGAACTACTAATAGGCGGTGGGATTGAAACTATCGCAATTACAGAAGCCTTTGGCGAATTTAGCTTCCTACTCACATGCATGCAGGGAATGGAAAGGTTGCTTACATCAGATACAGAAGGAACTTTTCGACCTGAAAGACTTGTACCAATAGCTGAAAGATTTGGCTTGGACCCAGGAGCTGTCCTCGACAAAAGCTGCTACTTTTCCTATTATATCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

136

Amino Acids

15.32

Weight (kDa)

4.99

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad51 PF08423 50 - 130 1.1e-15 Rad51
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 123
Acc36I ACCTGC 1 cut(s) 123
AccBSI CCGCTC 1 cut(s) 60
AccII CGCG 1 cut(s) 160
AciI CCGC 2 cut(s) 58, 204
AclWI GGATC 1 cut(s) 114
AcsI RAATTY 2 cut(s) 23, 245
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 1 cut(s) 333
AgsI TTSAA 3 cut(s) 23, 191, 215
AjnI CCWGG 1 cut(s) 361
AluBI AGCT 5 cut(s) 92, 252, 341, 368, 384
AluI AGCT 5 cut(s) 92, 252, 341, 368, 384
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 1 cut(s) 114
AlwNI CAGNNNCTG 1 cut(s) 368
ApeKI GCWGC 1 cut(s) 384
ApoI RAATTY 2 cut(s) 23, 245
AspLEI GCGC 2 cut(s) 160, 162
AspS9I GGNCC 1 cut(s) 358
AsuII TTCGAA 1 cut(s) 35
AvaII GGWCC 1 cut(s) 358
BbvI GCAGC 1 cut(s) 371
BciT130I CCWGG 1 cut(s) 363
BcoDI GTCTC 1 cut(s) 73
BfuAI ACCTGC 1 cut(s) 123
BisI GCNGC 1 cut(s) 385
BlsI GCNGC 1 cut(s) 386
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 1 cut(s) 358
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 1 cut(s) 360
BmrFI CCNGG 1 cut(s) 363
BmsI GCATC 1 cut(s) 121
BplI GAGNNNNNCTC 2 cut(s) 357, 389
Bpu14I TTCGAA 1 cut(s) 35
BpuEI CTTGAG 1 cut(s) 46
BsaJI CCNNGG 1 cut(s) 361
BsaWI WCCGGW 1 cut(s) 170
Bse3DI GCAATG 2 cut(s) 58, 125
BseBI CCWGG 1 cut(s) 363
BseDI CCNNGG 1 cut(s) 361
BseMI GCAATG 2 cut(s) 58, 125
BseMII CTCAG 1 cut(s) 115
BsePI GCGCGC 1 cut(s) 158
BseXI GCAGC 1 cut(s) 371
Bsh1236I CGCG 1 cut(s) 160
BsiSI CCGG 1 cut(s) 171
BsmAI GTCTC 1 cut(s) 73
Bsp119I TTCGAA 1 cut(s) 35
Bsp143I GATC 1 cut(s) 106
BspACI CCGC 2 cut(s) 58, 204
BspCNI CTCAG 1 cut(s) 114
BspFNI CGCG 1 cut(s) 160
BspLI GGNNCC 1 cut(s) 360
BspMI ACCTGC 1 cut(s) 123
BspPI GGATC 1 cut(s) 114
BspT104I TTCGAA 1 cut(s) 35
BsrBI CCGCTC 1 cut(s) 60
BsrDI GCAATG 2 cut(s) 58, 125
BssECI CCNNGG 1 cut(s) 361
BssHII GCGCGC 1 cut(s) 158
BssMI GATC 1 cut(s) 106
Bst2UI CCWGG 1 cut(s) 363
BstBI TTCGAA 1 cut(s) 35
BstC8I GCNNGC 4 cut(s) 11, 15, 160, 269
BstDEI CTNAG 1 cut(s) 101
BstFNI CGCG 1 cut(s) 160
BstHHI GCGC 2 cut(s) 160, 162
BstKTI GATC 1 cut(s) 109
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 1 cut(s) 106
BstMWI GCNNNNNNNGC 2 cut(s) 57, 249
BstNI CCWGG 1 cut(s) 363
BstNSI RCATGY 2 cut(s) 267, 271
BstSCI CCNGG 1 cut(s) 361
BstUI CGCG 1 cut(s) 160
BstV1I GCAGC 1 cut(s) 371
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
BveI ACCTGC 1 cut(s) 123
Cac8I GCNNGC 4 cut(s) 11, 15, 160, 269
CaiI CAGNNNCTG 1 cut(s) 368
CfoI GCGC 2 cut(s) 160, 162
Cfr13I GGNCC 1 cut(s) 358
Csp6I GTAC 1 cut(s) 332
CviAII CATG 2 cut(s) 264, 268
CviQI GTAC 1 cut(s) 332
DdeI CTNAG 1 cut(s) 101
DpnI GATC 1 cut(s) 108
DpnII GATC 1 cut(s) 106
Eco47I GGWCC 1 cut(s) 358
Eco57I CTGAAG 1 cut(s) 78
EcoRI GAATTC 1 cut(s) 23
EcoRII CCWGG 1 cut(s) 361
EcoT22I ATGCAT 1 cut(s) 269
FaeI CATG 2 cut(s) 267, 271
FaiI YATR 3 cut(s) 265, 269, 402
FatI CATG 2 cut(s) 263, 267
Fnu4HI GCNGC 1 cut(s) 385
Fsp4HI GCNGC 1 cut(s) 385
GlaI GCGC 2 cut(s) 159, 161
GluI GCNGC 1 cut(s) 385
HapII CCGG 1 cut(s) 171
HhaI GCGC 2 cut(s) 160, 162
Hin1II CATG 2 cut(s) 267, 271
Hin6I GCGC 2 cut(s) 158, 160
HinP1I GCGC 2 cut(s) 158, 160
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 2 cut(s) 67, 99
HpaII CCGG 1 cut(s) 171
Hpy188I TCNGA 1 cut(s) 298
Hpy188III TCNNGA 2 cut(s) 86, 96
HpyAV CCTTC 1 cut(s) 299
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 5 cut(s) 51, 118, 134, 267, 271
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 249
HpyF3I CTNAG 1 cut(s) 101
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 2 cut(s) 267, 271
HspAI GCGC 2 cut(s) 158, 160
Kzo9I GATC 1 cut(s) 106
LmnI GCTCC 1 cut(s) 365
LpnPI CCDG 9 cut(s) 81, 88, 89, 128, 184, 257, 333, 348, 375
Lsp1109I GCAGC 1 cut(s) 371
LweI GCATC 1 cut(s) 121
MaeII ACGT 1 cut(s) 39
MalI GATC 1 cut(s) 108
MbiI CCGCTC 1 cut(s) 60
MboI GATC 1 cut(s) 106
MflI RGATCY 1 cut(s) 106
MluCI AATT 4 cut(s) 23, 30, 225, 245
MlyI GAGTC 1 cut(s) 93
MnlI CCTC 1 cut(s) 383
Mph1103I ATGCAT 1 cut(s) 269
MslI CAYNNNNRTG 1 cut(s) 266
MspI CCGG 1 cut(s) 171
MspR9I CCNGG 1 cut(s) 363
MvaI CCWGG 1 cut(s) 363
MvnI CGCG 1 cut(s) 160
MwoI GCNNNNNNNGC 2 cut(s) 57, 249
NdeII GATC 1 cut(s) 106
NlaIII CATG 2 cut(s) 267, 271
NlaIV GGNNCC 1 cut(s) 360
NsiI ATGCAT 1 cut(s) 269
NspI RCATGY 2 cut(s) 267, 271
NspV TTCGAA 1 cut(s) 35
PaeI GCATGC 1 cut(s) 271
PaqCI CACCTGC 1 cut(s) 123
PauI GCGCGC 1 cut(s) 158
PfeI GAWTC 1 cut(s) 67
PkrI GCNGC 1 cut(s) 386
PleI GAGTC 1 cut(s) 93
PpsI GAGTC 1 cut(s) 93
Psp6I CCWGG 1 cut(s) 361
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 1 cut(s) 360
PspPI GGNCC 1 cut(s) 358
PstNI CAGNNNCTG 1 cut(s) 368
PsuI RGATCY 1 cut(s) 106
PteI GCGCGC 1 cut(s) 158
RsaI GTAC 1 cut(s) 333
RsaNI GTAC 1 cut(s) 332
RseI CAYNNNNRTG 1 cut(s) 266
SatI GCNGC 1 cut(s) 385
Sau3AI GATC 1 cut(s) 106
Sau96I GGNCC 1 cut(s) 358
SchI GAGTC 1 cut(s) 93
ScrFI CCNGG 1 cut(s) 363
SetI ASST 9 cut(s) 42, 94, 117, 254, 287, 322, 343, 370, 386
SfaNI GCATC 1 cut(s) 121
SfuI TTCGAA 1 cut(s) 35
SinI GGWCC 1 cut(s) 358
SmiMI CAYNNNNRTG 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
SphI GCATGC 1 cut(s) 271
Sse9I AATT 4 cut(s) 23, 30, 225, 245
SsiI CCGC 2 cut(s) 58, 204
StyD4I CCNGG 1 cut(s) 361
TaiI ACGT 1 cut(s) 42
TaqI TCGA 3 cut(s) 35, 316, 375
TasI AATT 4 cut(s) 23, 30, 225, 245
TfiI GAWTC 1 cut(s) 67
TseI GCWGC 1 cut(s) 384
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 2 cut(s) 23, 245
XceI RCATGY 2 cut(s) 267, 271
Zsp2I ATGCAT 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.