pycom05g15480

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001853
Physical Location & Seq
Forward (+)
19047815 .. 19049517
1703 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g15480.1

Sequence Viewer

Length: 1173 bp
ATGGCTTTTGCTCGAGATGAAAATCGAGAGACTGCACTTCATGTGATGGCACGAAAACATTCAGCGTATTATAGTGGACGCCAACCGGGTTTTGTGCAACCATGCATATCTTCAGTTCCTCGTATCAAGGTTTGCTACAACAAAATAGTAATGCAGACGCAAGCCACTGAGCTTGTGAAGCACTTGTGGAAGAAAGTACTTACTTTAGAAAATGATTCAAAAATTAGCGATTTAATCAGAGCACCCTCTCGCTTATTGTTCAGTGCTGCTGAGTTGGGGAACGTTGACTTCCTGGTCATACTTATAAGAACATACCCTAATCTTATTTGGAAAGTTGATGAACAAAATCGAACCATATTTCACACTGCAGTTGTTCACCGGCAGGAGAAGGTTTTCAATCTCATACATGAATTAGGTGGACTAAAAGATTTAATTGCATCTTACAAAGATGAAGACAATAACAATATGCTCCATTTGGCCGCCAAACTTGCACCGGATGATCGATTAAATACTGATACGGGGGCAGCATTGCAATTCAGAAGAGAGTTACAATGGTTTAAGGAAGTGGAGAAGATCGTCCAACCATTGTACAAGGAAATGAATAACTCAGATGGAAAAACACCTCAAGTCCTATTCACTGAAGAGCACAAGGACTTGTTACGAGAAGGAGAAAAGTGGATGAAGGGCACTGCATCATCGTGCATGCTTGTAGCAACGCTCATTGCCACCGTAATGTTTGCCGTTTTCTCCACAGTACCGGGTGGAAACAACGACGACACAGGCATTCCGATTTTTCTCCACTCACGGGCCTTCATGGTTTTCACCATATCAGACGCACTCTCCCTCGTCTCCGCCACCACATCAATCTTGAGTTTCCTGTCCATTCTTACATCACGTTATGCCGAAGGAGATTTCCTCCATTCACTACCGAATCGGTTAATTGTAGGATTGGCAACCCTTTTCATCTCTATAGTTACCATGATGATCACGTTCGTAGCGTCGCTATACATCGTTCTAGGCCATGGATATCAGACGATCAAAGTTGCAATAGCTTTGGCTGCGGGCGTTCCTGTTAGTTTCTATGCTCTGTTACAGTTTCCTCTTCTTGGAGATATGATCATTCATGCTTATATTTCAAGGGTATCCTTTCGTCCCATGGGAACACTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

391

Amino Acids

43.77

Weight (kDa)

8.6

Isoelectric Point (pI)

27.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 224 - 338 4.6e-25 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 305
AasI GACNNNNNNGTC 1 cut(s) 293
AciI CCGC 3 cut(s) 480, 852, 1061
AclI AACGTT 1 cut(s) 282
AcoI YGGCCR 1 cut(s) 477
AcuI CTGAAG 2 cut(s) 96, 660
AcyI GRCGYC 1 cut(s) 79
AfaI GTAC 3 cut(s) 198, 590, 756
AfiI CCNNNNNNNGG 2 cut(s) 805, 1106
AgsI TTSAA 3 cut(s) 219, 397, 1137
AjnI CCWGG 1 cut(s) 291
AluBI AGCT 2 cut(s) 172, 1052
AluI AGCT 2 cut(s) 172, 1052
Alw21I GWGCWC 2 cut(s) 244, 648
Alw26I GTCTC 2 cut(s) 23, 853
Ama87I CYCGRG 1 cut(s) 12
AoxI GGCC 3 cut(s) 477, 807, 1018
ApeKI GCWGC 3 cut(s) 266, 524, 1058
Asp700I GAANNNNTTC 2 cut(s) 58, 392
AspS9I GGNCC 1 cut(s) 807
AsuC2I CCSGG 2 cut(s) 87, 759
AsuHPI GGTGA 2 cut(s) 368, 814
AvaI CYCGRG 1 cut(s) 12
BaeGI GKGCMC 1 cut(s) 689
BbsI GAAGAC 1 cut(s) 459
Bbv12I GWGCWC 2 cut(s) 244, 648
BbvI GCAGC 3 cut(s) 253, 536, 1045
BccI CCATC 2 cut(s) 40, 605
BceAI ACGGC 1 cut(s) 725
BciT130I CCWGG 1 cut(s) 293
BciVI GTATCC 1 cut(s) 1153
BclI TGATCA 2 cut(s) 984, 1116
BcnI CCSGG 2 cut(s) 87, 759
BcoDI GTCTC 2 cut(s) 23, 853
BfaI CTAG 1 cut(s) 1016
BfmI CTRYAG 2 cut(s) 366, 969
BfuI GTATCC 1 cut(s) 1153
BisI GCNGC 4 cut(s) 267, 480, 525, 1059
BlsI GCNGC 4 cut(s) 268, 481, 526, 1060
BmcAI AGTACT 1 cut(s) 198
Bme1390I CCNGG 3 cut(s) 87, 293, 759
BmeT110I CYCGRG 1 cut(s) 12
BmgT120I GGNCC 1 cut(s) 807
BmrFI CCNGG 3 cut(s) 87, 293, 759
BmsI GCATC 2 cut(s) 446, 701
BpiI GAAGAC 1 cut(s) 459
BplI GAGNNNNNCTC 2 cut(s) 900, 932
BpuEI CTTGAG 2 cut(s) 609, 889
BpuMI CCSGG 2 cut(s) 87, 759
Bsa29I ATCGAT 1 cut(s) 502
BsaBI GATNNNNATC 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 79
BsaJI CCNNGG 2 cut(s) 1021, 1155
BsaWI WCCGGW 1 cut(s) 493
BsaXI ACNNNNNCTCC 2 cut(s) 824, 854
Bsc4I CCNNNNNNNGG 2 cut(s) 805, 1106
Bse118I RCCGGY 1 cut(s) 378
Bse3DI GCAATG 2 cut(s) 527, 720
Bse8I GATNNNNATC 1 cut(s) 21
BseBI CCWGG 1 cut(s) 293
BseCI ATCGAT 1 cut(s) 502
BseDI CCNNGG 2 cut(s) 1021, 1155
BseGI GGATG 2 cut(s) 502, 684
BseJI GATNNNNATC 1 cut(s) 21
BseLI CCNNNNNNNGG 2 cut(s) 805, 1106
BseMI GCAATG 2 cut(s) 527, 720
BseMII CTCAG 3 cut(s) 159, 261, 621
BseSI GKGCMC 1 cut(s) 689
BseXI GCAGC 3 cut(s) 253, 536, 1045
BsgI GTGCAG 1 cut(s) 18
BshFI GGCC 3 cut(s) 479, 809, 1020
BshVI ATCGAT 1 cut(s) 502
BsiHKAI GWGCWC 2 cut(s) 244, 648
BsiHKCI CYCGRG 1 cut(s) 12
BsiSI CCGG 4 cut(s) 86, 379, 494, 758
BslFI GGGAC 1 cut(s) 1137
BslI CCNNNNNNNGG 2 cut(s) 805, 1106
BsmAI GTCTC 2 cut(s) 23, 853
BsmBI CGTCTC 1 cut(s) 853
BsmFI GGGAC 1 cut(s) 1137
BsmI GAATGC 1 cut(s) 783
BsnI GGCC 3 cut(s) 479, 809, 1020
BsoBI CYCGRG 1 cut(s) 12
Bsp1286I GDGCHC 3 cut(s) 244, 648, 689
Bsp1407I TGTACA 1 cut(s) 588
Bsp143I GATC 5 cut(s) 499, 573, 984, 1035, 1116
Bsp19I CCATGG 2 cut(s) 1021, 1155
BspACI CCGC 3 cut(s) 480, 852, 1061
BspANI GGCC 3 cut(s) 479, 809, 1020
BspCNI CTCAG 3 cut(s) 160, 262, 620
BspDI ATCGAT 1 cut(s) 502
BspMAI CTGCAG 1 cut(s) 370
BspQI GCTCTTC 1 cut(s) 636
BsrDI GCAATG 2 cut(s) 527, 720
BsrFI RCCGGY 1 cut(s) 378
BsrGI TGTACA 1 cut(s) 588
BssAI RCCGGY 1 cut(s) 378
BssECI CCNNGG 2 cut(s) 1021, 1155
BssMI GATC 5 cut(s) 499, 573, 984, 1035, 1116
BssNI GRCGYC 1 cut(s) 79
BssT1I CCWWGG 2 cut(s) 1021, 1155
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 3 cut(s) 730, 754, 1095
Bst6I CTCTTC 3 cut(s) 535, 636, 1107
BstACI GRCGYC 1 cut(s) 79
BstAUI TGTACA 1 cut(s) 588
BstC8I GCNNGC 3 cut(s) 162, 704, 1063
BstDEI CTNAG 3 cut(s) 168, 270, 607
BstDSI CCRYGG 2 cut(s) 1021, 1155
BstF5I GGATG 2 cut(s) 502, 684
BstKTI GATC 5 cut(s) 502, 576, 987, 1038, 1119
BstMAI GTCTC 2 cut(s) 23, 853
BstMBI GATC 5 cut(s) 499, 573, 984, 1035, 1116
BstMWI GCNNNNNNNGC 3 cut(s) 178, 488, 1058
BstNI CCWGG 1 cut(s) 293
BstNSI RCATGY 1 cut(s) 706
BstSCI CCNGG 3 cut(s) 85, 291, 757
BstSFI CTRYAG 2 cut(s) 366, 969
BstSLI GKGCMC 1 cut(s) 689
BstV1I GCAGC 3 cut(s) 253, 536, 1045
BstV2I GAAGAC 1 cut(s) 459
Bsu15I ATCGAT 1 cut(s) 502
BsuI GTATCC 1 cut(s) 1153
BsuRI GGCC 3 cut(s) 479, 809, 1020
BsuTUI ATCGAT 1 cut(s) 502
BtgI CCRYGG 2 cut(s) 1021, 1155
BtsCI GGATG 2 cut(s) 502, 684
BtsI GCAGTG 2 cut(s) 363, 687
BtsIMutI CAGTG 5 cut(s) 165, 268, 363, 636, 687
Cac8I GCNNGC 3 cut(s) 162, 704, 1063
Cfr10I RCCGGY 1 cut(s) 378
Cfr13I GGNCC 1 cut(s) 807
ClaI ATCGAT 1 cut(s) 502
CseI GACGC 4 cut(s) 87, 166, 842, 987
Csp6I GTAC 3 cut(s) 197, 589, 755
CspCI CAANNNNNGTGG 2 cut(s) 154, 189
CviAII CATG 9 cut(s) 41, 102, 407, 703, 814, 979, 1022, 1124, 1156
CviJI RGCY 8 cut(s) 5, 164, 172, 479, 809, 1020, 1052, 1058
CviKI_1 RGCY 8 cut(s) 5, 164, 172, 479, 809, 1020, 1052, 1058
CviQI GTAC 3 cut(s) 197, 589, 755
DdeI CTNAG 3 cut(s) 168, 270, 607
DpnI GATC 5 cut(s) 501, 575, 986, 1037, 1118
DpnII GATC 5 cut(s) 499, 573, 984, 1035, 1116
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
EaeI YGGCCR 1 cut(s) 477
Eam1104I CTCTTC 3 cut(s) 535, 636, 1107
EarI CTCTTC 3 cut(s) 535, 636, 1107
EciI GGCGGA 1 cut(s) 841
Eco130I CCWWGG 2 cut(s) 1021, 1155
Eco32I GATATC 1 cut(s) 1028
Eco57I CTGAAG 2 cut(s) 96, 660
Eco88I CYCGRG 1 cut(s) 12
EcoRII CCWGG 1 cut(s) 291
EcoRV GATATC 1 cut(s) 1028
EcoT14I CCWWGG 2 cut(s) 1021, 1155
EcoT22I ATGCAT 1 cut(s) 107
ErhI CCWWGG 2 cut(s) 1021, 1155
Esp3I CGTCTC 1 cut(s) 853
FaeI CATG 9 cut(s) 44, 105, 410, 706, 817, 982, 1025, 1127, 1159
FaqI GGGAC 1 cut(s) 1137
FatI CATG 9 cut(s) 40, 101, 406, 702, 813, 978, 1021, 1123, 1155
FauI CCCGC 1 cut(s) 1054
FbaI TGATCA 2 cut(s) 984, 1116
Fnu4HI GCNGC 4 cut(s) 267, 480, 525, 1059
FokI GGATG 2 cut(s) 509, 691
Fsp4HI GCNGC 4 cut(s) 267, 480, 525, 1059
FspBI CTAG 1 cut(s) 1016
GluI GCNGC 4 cut(s) 267, 480, 525, 1059
HaeIII GGCC 3 cut(s) 479, 809, 1020
HapII CCGG 4 cut(s) 86, 379, 494, 758
HgaI GACGC 4 cut(s) 87, 166, 842, 987
Hin1I GRCGYC 1 cut(s) 79
Hin1II CATG 9 cut(s) 44, 105, 410, 706, 817, 982, 1025, 1127, 1159
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 2 cut(s) 215, 931
HpaII CCGG 4 cut(s) 86, 379, 494, 758
HphI GGTGA 2 cut(s) 368, 814
Hpy166II GTNNAC 4 cut(s) 77, 286, 376, 419
Hpy188I TCNGA 6 cut(s) 239, 539, 610, 789, 832, 1032
Hpy188III TCNNGA 3 cut(s) 14, 26, 868
Hpy8I GTNNAC 4 cut(s) 77, 286, 376, 419
Hpy99I CGWCG 2 cut(s) 776, 1003
HpyAV CCTTC 5 cut(s) 382, 659, 676, 820, 899
HpyCH4III ACNGT 3 cut(s) 730, 754, 1095
HpyCH4IV ACGT 3 cut(s) 282, 895, 989
HpyF10VI GCNNNNNNNGC 3 cut(s) 178, 488, 1058
HpyF3I CTNAG 3 cut(s) 168, 270, 607
HpySE526I ACGT 3 cut(s) 282, 895, 989
Hsp92I GRCGYC 1 cut(s) 79
Hsp92II CATG 9 cut(s) 44, 105, 410, 706, 817, 982, 1025, 1127, 1159
Ksp22I TGATCA 2 cut(s) 984, 1116
Kzo9I GATC 5 cut(s) 499, 573, 984, 1035, 1116
LguI GCTCTTC 1 cut(s) 636
LmnI GCTCC 1 cut(s) 474
Lsp1109I GCAGC 3 cut(s) 253, 536, 1045
LweI GCATC 2 cut(s) 446, 701
MaeI CTAG 1 cut(s) 1016
MaeII ACGT 3 cut(s) 282, 895, 989
MaeIII GTNAC 4 cut(s) 546, 657, 973, 1089
MalI GATC 5 cut(s) 501, 575, 986, 1037, 1118
MboI GATC 5 cut(s) 499, 573, 984, 1035, 1116
MboII GAAGA 7 cut(s) 102, 202, 464, 552, 583, 653, 1094
MhlI GDGCHC 3 cut(s) 244, 648, 689
MluCI AATT 5 cut(s) 222, 410, 432, 533, 939
MmeI TCCRAC 1 cut(s) 604
MnlI CCTC 6 cut(s) 129, 256, 633, 854, 926, 1110
Mph1103I ATGCAT 1 cut(s) 107
MroXI GAANNNNTTC 2 cut(s) 58, 392
MseI TTAA 5 cut(s) 233, 431, 506, 558, 938
MslI CAYNNNNRTG 2 cut(s) 697, 731
MspI CCGG 4 cut(s) 86, 379, 494, 758
MspR9I CCNGG 3 cut(s) 87, 293, 759
Mva1269I GAATGC 1 cut(s) 783
MvaI CCWGG 1 cut(s) 293
MwoI GCNNNNNNNGC 3 cut(s) 178, 488, 1058
NciI CCSGG 2 cut(s) 87, 759
NcoI CCATGG 2 cut(s) 1021, 1155
NdeII GATC 5 cut(s) 499, 573, 984, 1035, 1116
NlaIII CATG 9 cut(s) 44, 105, 410, 706, 817, 982, 1025, 1127, 1159
NsiI ATGCAT 1 cut(s) 107
NspI RCATGY 1 cut(s) 706
PaeI GCATGC 1 cut(s) 706
PaeR7I CTCGAG 1 cut(s) 12
PciSI GCTCTTC 1 cut(s) 636
PcsI WCGNNNNNNNCGW 1 cut(s) 995
PctI GAATGC 1 cut(s) 783
PdmI GAANNNNTTC 2 cut(s) 58, 392
PfeI GAWTC 2 cut(s) 215, 931
PkrI GCNGC 4 cut(s) 268, 481, 526, 1060
PsiI TTATAA 1 cut(s) 305
Psp1406I AACGTT 1 cut(s) 282
Psp6I CCWGG 1 cut(s) 291
PspGI CCWGG 1 cut(s) 291
PspPI GGNCC 1 cut(s) 807
PstI CTGCAG 1 cut(s) 370
RsaI GTAC 3 cut(s) 198, 590, 756
RsaNI GTAC 3 cut(s) 197, 589, 755
RseI CAYNNNNRTG 2 cut(s) 697, 731
SapI GCTCTTC 1 cut(s) 636
SaqAI TTAA 5 cut(s) 233, 431, 506, 558, 938
SatI GCNGC 4 cut(s) 267, 480, 525, 1059
Sau3AI GATC 5 cut(s) 499, 573, 984, 1035, 1116
Sau96I GGNCC 1 cut(s) 807
ScaI AGTACT 1 cut(s) 198
ScrFI CCNGG 3 cut(s) 87, 293, 759
SduI GDGCHC 3 cut(s) 244, 648, 689
SetI ASST 9 cut(s) 132, 174, 285, 393, 418, 625, 898, 992, 1054
SfaNI GCATC 2 cut(s) 446, 701
SfcI CTRYAG 2 cut(s) 366, 969
Sfr274I CTCGAG 1 cut(s) 12
SlaI CTCGAG 1 cut(s) 12
SmiMI CAYNNNNRTG 2 cut(s) 697, 731
SmlI CTYRAG 3 cut(s) 12, 624, 868
SmoI CTYRAG 3 cut(s) 12, 624, 868
SphI GCATGC 1 cut(s) 706
Sse9I AATT 5 cut(s) 222, 410, 432, 533, 939
SsiI CCGC 3 cut(s) 480, 852, 1061
SspMI CTAG 1 cut(s) 1016
StyD4I CCNGG 3 cut(s) 85, 291, 757
StyI CCWWGG 2 cut(s) 1021, 1155
TaaI ACNGT 3 cut(s) 730, 754, 1095
TaiI ACGT 3 cut(s) 285, 898, 992
TaqI TCGA 4 cut(s) 13, 25, 349, 502
TasI AATT 5 cut(s) 222, 410, 432, 533, 939
TatI WGTACW 2 cut(s) 196, 588
TauI GCSGC 1 cut(s) 482
TfiI GAWTC 2 cut(s) 215, 931
Tru1I TTAA 5 cut(s) 233, 431, 506, 558, 938
Tru9I TTAA 5 cut(s) 233, 431, 506, 558, 938
TscAI CASTG 5 cut(s) 172, 268, 370, 643, 694
TseI GCWGC 3 cut(s) 266, 524, 1058
TspRI CASTG 5 cut(s) 172, 268, 370, 643, 694
XceI RCATGY 1 cut(s) 706
XhoI CTCGAG 1 cut(s) 12
XmnI GAANNNNTTC 2 cut(s) 58, 392
XspI CTAG 1 cut(s) 1016
ZrmI AGTACT 1 cut(s) 198
Zsp2I ATGCAT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.