Rroxscaffold_2G00131190

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001853
Physical Location & Seq
Reverse (-)
67727614 .. 67729517
1904 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00131190.1

Sequence Viewer

Length: 1287 bp
ATGATAATTGCAGATGTAGCTCTGGATATTGTTAAACAACACCCTGAAATGGCTCTTGCTCGAGATGAAAACGAAGAGACTGCACTCCATGTAATGGCGCGAATGCCTTCAGCATATCATAATGGAAGTCAGATTGGATTTCGGAAACGATGCATATCATCTTTCCCCAGGAATTTCAAGCATTCCTGCGAAGAAAAGGTAAAACATGCACTAGCCACTGAACTTGTGAAGCAGCTGTGGAAGAAAATTCTGACATTAGAAAATGATTCCAAGATTGGTGATTTGATCAGAAAACCGTCTCGCTTATTGTTTACTGCAGCAGAGTTGGGTAACCTTGACTTTTTGATCATACTTATGAGATCATACCCTAATCTCATTTGGAAAGTTGATGAACAAAATCGAAGCATATTTCACATTGCAGTGGTTCACAGGCAGGAGAAGGTTTTCAATCTTATTTATGAATTAGGTGGATTGAAAGATTTGATAGCATCTTATAAAGATGACAACAATAACAATATGCTTCATTTGGCTGCAAAACTTGCACCTGCCAATAGACTTAATACTGATACTGGAGCAGCATTTCAACTGAGAAGAGAATTGTTGTGGTTTAAGGTATCCAGTCCTGAGTCTGGGAATATTCAAAATTTGATGTATGTGAGGCCTTTATGTCAGGAAGTAGAGAAGATCGTCCAACCGTTATACAAAGAGATGAGGAACTCTGATGGCAAAACACCTCAAATTCTATTCACTGAGGAGCACAGGGAATTACTAAAAGAAGGAGAGAAGTGGATGAAGGGCACTGCATCATCATGCATGGTTGTGGCAACCCTCATCGCCACCGTAATGTTTGCTGTTTTCTCCACTGTCCCGGGTGGCAACAACAATGACACAGGCATGCCCATGTTCATAGAAAAAACGGCCTTCAAAATCTTTGCCATATCAGATGCAGTATCACTCGTCTCTTCTGCCACTTCAATCTTGAGTTTTCTCTCAATCCTAACCTCACGCTACACCGAAGGTGACTTCCTCCATTCTTTACCAAACAGGTTGATCGTAGGGCTTGCAACCCTATTCATCTCTATAGCTACCATGATGCTCACGTACGTTGCAACGTTATTTATTGTTCTCGGCTCTGGAAATGAAGGGATAAAAGTTCCGATAACTTTGGTTGCTGTCATTCCAGTTAGTTTCTATGCTTTGCTGCAATTTCCTCTTCTTTCGGATATGATCAGTCACGCTTATATTTCAAGAGTATCTTTTCGTTCTAGCAACCATCTCCTGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

428

Amino Acids

48.11

Weight (kDa)

8.7

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 261 - 375 6.2e-25 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 495
AarI CACCTGC 1 cut(s) 553
Acc36I ACCTGC 1 cut(s) 553
AccB7I CCANNNNNTGG 1 cut(s) 94
AccII CGCG 1 cut(s) 100
AclI AACGTT 1 cut(s) 1112
AcsI RAATTY 4 cut(s) 172, 246, 643, 738
AcuI CTGAAG 1 cut(s) 93
AfaI GTAC 1 cut(s) 1103
AfiI CCNNNNNNNGG 3 cut(s) 49, 94, 629
AgsI TTSAA 8 cut(s) 178, 448, 475, 584, 641, 925, 975, 1248
AjnI CCWGG 1 cut(s) 167
AluBI AGCT 3 cut(s) 20, 235, 1085
AluI AGCT 3 cut(s) 20, 235, 1085
Alw21I GWGCWC 1 cut(s) 759
Alw26I GTCTC 3 cut(s) 71, 303, 964
Ama87I CYCGRG 2 cut(s) 60, 868
AoxI GGCC 2 cut(s) 659, 918
ApeKI GCWGC 5 cut(s) 232, 317, 530, 575, 1201
ApoI RAATTY 4 cut(s) 172, 246, 643, 738
Asp700I GAANNNNTTC 2 cut(s) 106, 443
AspLEI GCGC 1 cut(s) 100
AsuC2I CCSGG 2 cut(s) 869, 870
AsuHPI GGTGA 2 cut(s) 290, 1031
AvaI CYCGRG 2 cut(s) 60, 868
BaeGI GKGCMC 1 cut(s) 800
Bbv12I GWGCWC 1 cut(s) 759
BbvI GCAGC 5 cut(s) 244, 329, 517, 587, 1188
BccI CCATC 2 cut(s) 716, 1281
BceAI ACGGC 1 cut(s) 933
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 1 cut(s) 169
BciVI GTATCC 1 cut(s) 625
BclI TGATCA 3 cut(s) 285, 345, 1227
BcnI CCSGG 2 cut(s) 869, 870
BcoDI GTCTC 3 cut(s) 71, 303, 964
BfaI CTAG 2 cut(s) 212, 1266
BfmI CTRYAG 2 cut(s) 315, 1080
BfuAI ACCTGC 1 cut(s) 553
BfuI GTATCC 1 cut(s) 625
BisI GCNGC 5 cut(s) 233, 318, 531, 576, 1202
BlsI GCNGC 5 cut(s) 234, 319, 532, 577, 1203
Bme1390I CCNGG 3 cut(s) 169, 869, 870
BmeT110I CYCGRG 2 cut(s) 60, 868
BmrFI CCNGG 3 cut(s) 169, 869, 870
BmsI GCATC 5 cut(s) 140, 497, 812, 934, 1083
BpmI CTGGAG 1 cut(s) 591
BpuEI CTTGAG 1 cut(s) 1000
BpuMI CCSGG 2 cut(s) 869, 870
BsaAI YACGTR 1 cut(s) 1101
BsaBI GATNNNNATC 1 cut(s) 154
BsaJI CCNNGG 2 cut(s) 167, 868
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 94, 629
Bse1I ACTGG 3 cut(s) 574, 618, 1181
Bse3DI GCAATG 1 cut(s) 414
Bse8I GATNNNNATC 1 cut(s) 154
BseBI CCWGG 1 cut(s) 169
BseDI CCNNGG 2 cut(s) 167, 868
BseGI GGATG 1 cut(s) 795
BseJI GATNNNNATC 1 cut(s) 154
BseLI CCNNNNNNNGG 3 cut(s) 49, 94, 629
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 3 cut(s) 578, 615, 741
BseNI ACTGG 3 cut(s) 574, 618, 1181
BseRI GAGGAG 1 cut(s) 767
BseSI GKGCMC 1 cut(s) 800
BseXI GCAGC 5 cut(s) 244, 329, 517, 587, 1188
BsgI GTGCAG 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 100
BshFI GGCC 2 cut(s) 661, 920
BsiHKAI GWGCWC 1 cut(s) 759
BsiHKCI CYCGRG 2 cut(s) 60, 868
BsiSI CCGG 1 cut(s) 869
BsiWI CGTACG 1 cut(s) 1101
BslFI GGGAC 1 cut(s) 851
BslI CCNNNNNNNGG 3 cut(s) 49, 94, 629
BsmAI GTCTC 3 cut(s) 71, 303, 964
BsmBI CGTCTC 2 cut(s) 303, 964
BsmFI GGGAC 1 cut(s) 851
BsmI GAATGC 2 cut(s) 108, 181
BsnI GGCC 2 cut(s) 661, 920
BsoBI CYCGRG 2 cut(s) 60, 868
Bsp1286I GDGCHC 2 cut(s) 759, 800
Bsp143I GATC 6 cut(s) 285, 345, 359, 684, 1050, 1227
BspANI GGCC 2 cut(s) 661, 920
BspCNI CTCAG 3 cut(s) 579, 616, 742
BspFNI CGCG 1 cut(s) 100
BspMAI CTGCAG 1 cut(s) 319
BspMI ACCTGC 1 cut(s) 553
BsrDI GCAATG 1 cut(s) 414
BsrI ACTGG 3 cut(s) 574, 618, 1181
BssECI CCNNGG 2 cut(s) 167, 868
BssMI GATC 6 cut(s) 285, 345, 359, 684, 1050, 1227
Bst2UI CCWGG 1 cut(s) 169
Bst4CI ACNGT 4 cut(s) 297, 696, 841, 865
Bst6I CTCTTC 4 cut(s) 69, 586, 967, 1218
BstAPI GCANNNNNTGC 1 cut(s) 539
BstBAI YACGTR 1 cut(s) 1101
BstC8I GCNNGC 2 cut(s) 896, 1062
BstDEI CTNAG 3 cut(s) 587, 624, 750
BstEII GGTNACC 1 cut(s) 329
BstF5I GGATG 1 cut(s) 795
BstFNI CGCG 1 cut(s) 100
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 6 cut(s) 288, 348, 362, 687, 1053, 1230
BstMAI GTCTC 3 cut(s) 71, 303, 964
BstMBI GATC 6 cut(s) 285, 345, 359, 684, 1050, 1227
BstMWI GCNNNNNNNGC 2 cut(s) 17, 539
BstNI CCWGG 1 cut(s) 169
BstNSI RCATGY 2 cut(s) 209, 898
BstPI GGTNACC 1 cut(s) 329
BstSCI CCNGG 3 cut(s) 167, 867, 868
BstSFI CTRYAG 2 cut(s) 315, 1080
BstSLI GKGCMC 1 cut(s) 800
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 5 cut(s) 244, 329, 517, 587, 1188
BsuI GTATCC 1 cut(s) 625
BsuRI GGCC 2 cut(s) 661, 920
BtgZI GCGATG 1 cut(s) 817
BtsCI GGATG 1 cut(s) 795
BtsI GCAGTG 2 cut(s) 426, 798
BtsIMutI CAGTG 5 cut(s) 216, 426, 747, 798, 861
BveI ACCTGC 1 cut(s) 553
Cac8I GCNNGC 2 cut(s) 896, 1062
CfoI GCGC 1 cut(s) 100
Cfr9I CCCGGG 1 cut(s) 868
Csp6I GTAC 1 cut(s) 1102
CspCI CAANNNNNGTGG 2 cut(s) 205, 240
CviAII CATG 7 cut(s) 89, 206, 810, 814, 895, 901, 1090
CviQI GTAC 1 cut(s) 1102
DdeI CTNAG 3 cut(s) 587, 624, 750
DpnI GATC 6 cut(s) 287, 347, 361, 686, 1052, 1229
DpnII GATC 6 cut(s) 285, 345, 359, 684, 1050, 1227
Eam1104I CTCTTC 4 cut(s) 69, 586, 967, 1218
EarI CTCTTC 4 cut(s) 69, 586, 967, 1218
Eco147I AGGCCT 1 cut(s) 661
Eco57I CTGAAG 1 cut(s) 93
Eco88I CYCGRG 2 cut(s) 60, 868
Eco91I GGTNACC 1 cut(s) 329
EcoO65I GGTNACC 1 cut(s) 329
EcoRII CCWGG 1 cut(s) 167
EcoT22I ATGCAT 2 cut(s) 155, 815
Esp3I CGTCTC 2 cut(s) 303, 964
FaeI CATG 7 cut(s) 92, 209, 813, 817, 898, 904, 1093
FalI AAGNNNNNCTT 2 cut(s) 1240, 1272
FaqI GGGAC 1 cut(s) 851
FatI CATG 7 cut(s) 88, 205, 809, 813, 894, 900, 1089
FbaI TGATCA 3 cut(s) 285, 345, 1227
Fnu4HI GCNGC 5 cut(s) 233, 318, 531, 576, 1202
FokI GGATG 1 cut(s) 802
Fsp4HI GCNGC 5 cut(s) 233, 318, 531, 576, 1202
FspBI CTAG 2 cut(s) 212, 1266
GlaI GCGC 1 cut(s) 99
GluI GCNGC 5 cut(s) 233, 318, 531, 576, 1202
GsuI CTGGAG 1 cut(s) 591
HaeIII GGCC 2 cut(s) 661, 920
HapII CCGG 1 cut(s) 869
HhaI GCGC 1 cut(s) 100
Hin1II CATG 7 cut(s) 92, 209, 813, 817, 898, 904, 1093
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HinfI GANTC 2 cut(s) 266, 626
HpaII CCGG 1 cut(s) 869
HphI GGTGA 2 cut(s) 290, 1031
Hpy166II GTNNAC 2 cut(s) 312, 427
Hpy188I TCNGA 8 cut(s) 132, 144, 252, 290, 721, 943, 1158, 1222
Hpy188III TCNNGA 7 cut(s) 23, 62, 623, 671, 979, 1134, 1248
Hpy8I GTNNAC 2 cut(s) 312, 427
HpyAV CCTTC 7 cut(s) 117, 433, 770, 787, 931, 1010, 1136
HpyCH4III ACNGT 4 cut(s) 297, 696, 841, 865
HpyCH4IV ACGT 3 cut(s) 1100, 1104, 1112
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 539
HpyF3I CTNAG 3 cut(s) 587, 624, 750
HpySE526I ACGT 3 cut(s) 1100, 1104, 1112
Hsp92II CATG 7 cut(s) 92, 209, 813, 817, 898, 904, 1093
HspAI GCGC 1 cut(s) 98
Ksp22I TGATCA 3 cut(s) 285, 345, 1227
Kzo9I GATC 6 cut(s) 285, 345, 359, 684, 1050, 1227
LmnI GCTCC 2 cut(s) 572, 754
Lsp1109I GCAGC 5 cut(s) 244, 329, 517, 587, 1188
LweI GCATC 5 cut(s) 140, 497, 812, 934, 1083
MaeI CTAG 2 cut(s) 212, 1266
MaeII ACGT 3 cut(s) 1100, 1104, 1112
MaeIII GTNAC 3 cut(s) 329, 1019, 1232
MalI GATC 6 cut(s) 287, 347, 361, 686, 1052, 1229
MboI GATC 6 cut(s) 285, 345, 359, 684, 1050, 1227
MboII GAAGA 7 cut(s) 86, 203, 253, 603, 694, 954, 1205
MhlI GDGCHC 2 cut(s) 759, 800
MluCI AATT 9 cut(s) 6, 172, 246, 461, 596, 643, 738, 764, 1205
MlyI GAGTC 1 cut(s) 635
MmeI TCCRAC 1 cut(s) 715
MnlI CCTC 8 cut(s) 651, 705, 744, 745, 839, 1012, 1037, 1221
Mph1103I ATGCAT 2 cut(s) 155, 815
MroXI GAANNNNTTC 2 cut(s) 106, 443
MseI TTAA 3 cut(s) 33, 558, 609
MslI CAYNNNNRTG 7 cut(s) 353, 419, 808, 818, 842, 893, 899
MspA1I CMGCKG 1 cut(s) 235
MspI CCGG 1 cut(s) 869
MspR9I CCNGG 3 cut(s) 169, 869, 870
Mva1269I GAATGC 2 cut(s) 108, 181
MvaI CCWGG 1 cut(s) 169
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 2 cut(s) 17, 539
NciI CCSGG 2 cut(s) 869, 870
NdeII GATC 6 cut(s) 285, 345, 359, 684, 1050, 1227
NlaIII CATG 7 cut(s) 92, 209, 813, 817, 898, 904, 1093
NmeAIII GCCGAG 1 cut(s) 1107
NmuCI GTSAC 2 cut(s) 1019, 1232
NsiI ATGCAT 2 cut(s) 155, 815
NspI RCATGY 2 cut(s) 209, 898
PaeI GCATGC 1 cut(s) 898
PaeR7I CTCGAG 1 cut(s) 60
PaqCI CACCTGC 1 cut(s) 553
PceI AGGCCT 1 cut(s) 661
PctI GAATGC 2 cut(s) 108, 181
PdmI GAANNNNTTC 2 cut(s) 106, 443
PfeI GAWTC 1 cut(s) 266
Pfl23II CGTACG 1 cut(s) 1101
PflMI CCANNNNNTGG 1 cut(s) 94
PkrI GCNGC 5 cut(s) 234, 319, 532, 577, 1203
PleI GAGTC 1 cut(s) 634
PpsI GAGTC 1 cut(s) 634
Ppu21I YACGTR 1 cut(s) 1101
PsiI TTATAA 1 cut(s) 495
Psp1406I AACGTT 1 cut(s) 1112
Psp6I CCWGG 1 cut(s) 167
PspEI GGTNACC 1 cut(s) 329
PspGI CCWGG 1 cut(s) 167
PspLI CGTACG 1 cut(s) 1101
PstI CTGCAG 1 cut(s) 319
PvuII CAGCTG 1 cut(s) 235
RsaI GTAC 1 cut(s) 1103
RsaNI GTAC 1 cut(s) 1102
RseI CAYNNNNRTG 7 cut(s) 353, 419, 808, 818, 842, 893, 899
SaqAI TTAA 3 cut(s) 33, 558, 609
SatI GCNGC 5 cut(s) 233, 318, 531, 576, 1202
Sau3AI GATC 6 cut(s) 285, 345, 359, 684, 1050, 1227
SchI GAGTC 1 cut(s) 635
ScrFI CCNGG 3 cut(s) 169, 869, 870
SduI GDGCHC 2 cut(s) 759, 800
SfaNI GCATC 5 cut(s) 140, 497, 812, 934, 1083
SfcI CTRYAG 2 cut(s) 315, 1080
Sfr274I CTCGAG 1 cut(s) 60
SlaI CTCGAG 1 cut(s) 60
SmaI CCCGGG 1 cut(s) 870
SmiMI CAYNNNNRTG 7 cut(s) 353, 419, 808, 818, 842, 893, 899
SmlI CTYRAG 2 cut(s) 60, 979
SmoI CTYRAG 2 cut(s) 60, 979
SphI GCATGC 1 cut(s) 898
Sse9I AATT 9 cut(s) 6, 172, 246, 461, 596, 643, 738, 764, 1205
SseBI AGGCCT 1 cut(s) 661
SspI AATATT 1 cut(s) 637
SspMI CTAG 2 cut(s) 212, 1266
StuI AGGCCT 1 cut(s) 661
StyD4I CCNGG 3 cut(s) 167, 867, 868
TaaI ACNGT 4 cut(s) 297, 696, 841, 865
TaiI ACGT 3 cut(s) 1103, 1107, 1115
TaqI TCGA 2 cut(s) 61, 400
TasI AATT 9 cut(s) 6, 172, 246, 461, 596, 643, 738, 764, 1205
TfiI GAWTC 1 cut(s) 266
Tru1I TTAA 3 cut(s) 33, 558, 609
Tru9I TTAA 3 cut(s) 33, 558, 609
TscAI CASTG 5 cut(s) 223, 426, 754, 805, 868
TseFI GTSAC 2 cut(s) 1019, 1232
TseI GCWGC 5 cut(s) 232, 317, 530, 575, 1201
Tsp45I GTSAC 2 cut(s) 1019, 1232
TspDTI ATGAA 8 cut(s) 81, 405, 474, 512, 806, 895, 1063, 1155
TspMI CCCGGG 1 cut(s) 868
TspRI CASTG 5 cut(s) 223, 426, 754, 805, 868
Van91I CCANNNNNTGG 1 cut(s) 94
XapI RAATTY 4 cut(s) 172, 246, 643, 738
XceI RCATGY 2 cut(s) 209, 898
XhoI CTCGAG 1 cut(s) 60
XmaI CCCGGG 1 cut(s) 868
XmnI GAANNNNTTC 2 cut(s) 106, 443
XspI CTAG 2 cut(s) 212, 1266
Zsp2I ATGCAT 2 cut(s) 155, 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.