pycom05g17200
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003940
Physical Location & Seq
Forward (+)
20415308 .. 20416447
1140 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g17200.2

Sequence Viewer

Length: 423 bp
ATGTGGATGGTATTAGGAAGAGTGGCAAGGAGTGCTCTAGTGCTGGTGGGATTTTACGTGTTGCTCTGGCCAAGTTCAAGCTGCTCTTCTTCAATGGAGATCAGTTGTAATCTTGAGAAAGCAGGCCATAGTTTTAAACGAAGTTCTTCCTTTTGGATTGACAATAGCAGCGATGTTTTCTCAACGTCTTCACGTGGAGAGGACGATGAGGAAGCTCTCAAATGGGCTGCTCTTCAGAGACTTCCGACTTTTCAGCGCTTGAACAAAGGCCTGCTCTCTACTCCTCAAGGCCATGCCAACGAAGTTGGCGTGAGCAATCTTGAAGTGCAAGACAGGAAGAACTTGATTGAGCGGTTAGTTGGAGTAGCTGAGGAGGATCATGAGAACTTCTTGTTGAGACTCAAGAGCCACATTGATAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

141

Amino Acids

15.77

Weight (kDa)

6.07

Isoelectric Point (pI)

59.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 352
AciI CCGC 1 cut(s) 352
AclWI GGATC 1 cut(s) 384
AcoI YGGCCR 1 cut(s) 68
AcuI CTGAAG 1 cut(s) 218
AcvI CACGTG 1 cut(s) 194
AfeI AGCGCT 1 cut(s) 257
AflIII ACRYGT 1 cut(s) 57
AgsI TTSAA 4 cut(s) 78, 93, 262, 323
AluBI AGCT 3 cut(s) 81, 215, 368
AluI AGCT 3 cut(s) 81, 215, 368
Alw21I GWGCWC 1 cut(s) 37
Alw26I GTCTC 2 cut(s) 232, 391
AlwI GGATC 1 cut(s) 384
Aor51HI AGCGCT 1 cut(s) 257
AoxI GGCC 4 cut(s) 68, 124, 268, 289
ApeKI GCWGC 3 cut(s) 81, 168, 227
Asp700I GAANNNNTTC 1 cut(s) 145
AspLEI GCGC 1 cut(s) 258
BalI TGGCCA 1 cut(s) 70
BbrPI CACGTG 1 cut(s) 194
BbsI GAAGAC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 37
BbvCI CCTCAGC 1 cut(s) 369
BbvI GCAGC 3 cut(s) 68, 180, 214
BcoDI GTCTC 2 cut(s) 232, 391
BfaI CTAG 1 cut(s) 38
BfoI RGCGCY 1 cut(s) 259
BisI GCNGC 3 cut(s) 82, 169, 228
BlsI GCNGC 3 cut(s) 83, 170, 229
BpiI GAAGAC 1 cut(s) 180
Bpu10I CCTNAGC 1 cut(s) 369
BpuEI CTTGAG 3 cut(s) 134, 270, 386
BsaAI YACGTR 2 cut(s) 58, 194
BseGI GGATG 1 cut(s) 12
BseMII CTCAG 1 cut(s) 360
BseRI GAGGAG 2 cut(s) 273, 386
BseXI GCAGC 3 cut(s) 68, 180, 214
BshFI GGCC 4 cut(s) 70, 126, 270, 291
BsiHKAI GWGCWC 1 cut(s) 37
BsmAI GTCTC 2 cut(s) 232, 391
BsnI GGCC 4 cut(s) 70, 126, 270, 291
Bsp1286I GDGCHC 1 cut(s) 37
Bsp143I GATC 2 cut(s) 99, 376
BspACI CCGC 1 cut(s) 352
BspANI GGCC 4 cut(s) 70, 126, 270, 291
BspCNI CTCAG 1 cut(s) 361
BspHI TCATGA 1 cut(s) 379
BspPI GGATC 1 cut(s) 384
BspQI GCTCTTC 2 cut(s) 91, 237
BsrBI CCGCTC 1 cut(s) 352
BssMI GATC 2 cut(s) 99, 376
Bst6I CTCTTC 3 cut(s) 13, 91, 237
BstAPI GCANNNNNTGC 1 cut(s) 32
BstBAI YACGTR 2 cut(s) 58, 194
BstC8I GCNNGC 2 cut(s) 124, 272
BstDEI CTNAG 1 cut(s) 369
BstF5I GGATG 1 cut(s) 12
BstH2I RGCGCY 1 cut(s) 259
BstHHI GCGC 1 cut(s) 258
BstKTI GATC 2 cut(s) 102, 379
BstMAI GTCTC 2 cut(s) 232, 391
BstMBI GATC 2 cut(s) 99, 376
BstMWI GCNNNNNNNGC 1 cut(s) 32
BstV1I GCAGC 3 cut(s) 68, 180, 214
BstV2I GAAGAC 1 cut(s) 180
BsuRI GGCC 4 cut(s) 70, 126, 270, 291
BtgZI GCGATG 1 cut(s) 186
BtsCI GGATG 1 cut(s) 12
Cac8I GCNNGC 2 cut(s) 124, 272
CciI TCATGA 1 cut(s) 379
CfoI GCGC 1 cut(s) 258
CviAII CATG 2 cut(s) 293, 380
CviJI RGCY 9 cut(s) 70, 81, 126, 215, 227, 270, 291, 368, 408
CviKI_1 RGCY 9 cut(s) 70, 81, 126, 215, 227, 270, 291, 368, 408
DdeI CTNAG 1 cut(s) 369
DpnI GATC 2 cut(s) 101, 378
DpnII GATC 2 cut(s) 99, 376
DraI TTTAAA 1 cut(s) 136
EaeI YGGCCR 1 cut(s) 68
Eam1104I CTCTTC 3 cut(s) 13, 91, 237
EarI CTCTTC 3 cut(s) 13, 91, 237
Eco147I AGGCCT 1 cut(s) 270
Eco47III AGCGCT 1 cut(s) 257
Eco57I CTGAAG 1 cut(s) 218
Eco72I CACGTG 1 cut(s) 194
FaeI CATG 2 cut(s) 296, 383
FaiI YATR 3 cut(s) 129, 294, 381
FalI AAGNNNNNCTT 2 cut(s) 70, 102
FatI CATG 2 cut(s) 292, 379
Fnu4HI GCNGC 3 cut(s) 82, 169, 228
FokI GGATG 1 cut(s) 19
Fsp4HI GCNGC 3 cut(s) 82, 169, 228
FspBI CTAG 1 cut(s) 38
GlaI GCGC 1 cut(s) 257
GluI GCNGC 3 cut(s) 82, 169, 228
HaeII RGCGCY 1 cut(s) 259
HaeIII GGCC 4 cut(s) 70, 126, 270, 291
HhaI GCGC 1 cut(s) 258
Hin1II CATG 2 cut(s) 296, 383
Hin6I GCGC 1 cut(s) 256
HinP1I GCGC 1 cut(s) 256
HinfI GANTC 1 cut(s) 399
Hpy188I TCNGA 2 cut(s) 237, 246
Hpy188III TCNNGA 4 cut(s) 113, 320, 380, 403
HpyCH4IV ACGT 3 cut(s) 57, 185, 193
HpyCH4V TGCA 1 cut(s) 328
HpyF10VI GCNNNNNNNGC 1 cut(s) 32
HpyF3I CTNAG 1 cut(s) 369
HpySE526I ACGT 3 cut(s) 57, 185, 193
Hsp92II CATG 2 cut(s) 296, 383
HspAI GCGC 1 cut(s) 256
Kzo9I GATC 2 cut(s) 99, 376
LguI GCTCTTC 2 cut(s) 91, 237
LpnPI CCDG 5 cut(s) 29, 52, 108, 284, 319
Lsp1109I GCAGC 3 cut(s) 68, 180, 214
MaeI CTAG 1 cut(s) 38
MaeII ACGT 3 cut(s) 57, 185, 193
MalI GATC 2 cut(s) 101, 378
MbiI CCGCTC 1 cut(s) 352
MboI GATC 2 cut(s) 99, 376
MboII GAAGA 7 cut(s) 30, 78, 81, 138, 180, 224, 349
MhlI GDGCHC 1 cut(s) 37
MlsI TGGCCA 1 cut(s) 70
MluNI TGGCCA 1 cut(s) 70
MlyI GAGTC 1 cut(s) 393
MmeI TCCRAC 2 cut(s) 269, 340
MnlI CCTC 5 cut(s) 193, 202, 294, 364, 367
Mox20I TGGCCA 1 cut(s) 70
MroXI GAANNNNTTC 1 cut(s) 145
MscI TGGCCA 1 cut(s) 70
MseI TTAA 1 cut(s) 135
Msp20I TGGCCA 1 cut(s) 70
MwoI GCNNNNNNNGC 1 cut(s) 32
NdeII GATC 2 cut(s) 99, 376
NlaIII CATG 2 cut(s) 296, 383
PagI TCATGA 1 cut(s) 379
PceI AGGCCT 1 cut(s) 270
PciSI GCTCTTC 2 cut(s) 91, 237
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PdmI GAANNNNTTC 1 cut(s) 145
PkrI GCNGC 3 cut(s) 83, 170, 229
PleI GAGTC 1 cut(s) 393
PmaCI CACGTG 1 cut(s) 194
PmlI CACGTG 1 cut(s) 194
PpsI GAGTC 1 cut(s) 393
Ppu21I YACGTR 2 cut(s) 58, 194
PspCI CACGTG 1 cut(s) 194
SapI GCTCTTC 2 cut(s) 91, 237
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 3 cut(s) 82, 169, 228
Sau3AI GATC 2 cut(s) 99, 376
SchI GAGTC 1 cut(s) 393
SduI GDGCHC 1 cut(s) 37
SetI ASST 7 cut(s) 60, 83, 188, 196, 217, 370, 422
SmlI CTYRAG 3 cut(s) 113, 285, 401
SmoI CTYRAG 3 cut(s) 113, 285, 401
SseBI AGGCCT 1 cut(s) 270
SsiI CCGC 1 cut(s) 352
SspMI CTAG 1 cut(s) 38
StuI AGGCCT 1 cut(s) 270
TaiI ACGT 3 cut(s) 60, 188, 196
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TseI GCWGC 3 cut(s) 81, 168, 227
XmnI GAANNNNTTC 1 cut(s) 145
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.