Rh5BG372500

Pleiotropic drug resistance protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003940
Physical Location & Seq
Forward (+)
58530396 .. 58536966
6571 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG372500.1

Sequence Viewer

Length: 1458 bp
ATGGCGGCGGCAGCAATGGCCGGAGAAGACTTAATAAGGCAGTCGAGCAGCCGGAGGAGCTCGCTGTCGAGCAGCCGGAGGAGCTGGCGGTCGACGAGTGTCCGAGAAATGTGGAACGCGCCGGACGTGTTCCAGCGGAGCGTCCGGCAGCCGGCGCTGGACGAGGAGGAGGAGCTCAAGTGGGCGGCCATAGAGCGGTTGCCGACTTATGATAGGATGAGGAGAGGCATGTTGAGGAATGTGATGAGCAATGGAAGGGTTGTGGCTGAAGAAGTTGACGTTGCGCATCTTGGGGATCAAGATAAGAAGCAGTTGATGGAGAGCATACTCAAGGTTATTGAGGAGGATAATGAAAAGTTCTTGAAGAAGCTCAGGGCCAGAAATGACAGGGTTGGCATCGATATTCCTAAAGTTGAGGTTAGATTCCAGAATTTATCAATAGATGGAGATGCGTTTGTAGGCACCAGAGCACTTCCAACTCTATGGAATTCCACCTTGAACACAATAGAGGGACTGCTGGGACTTGTTGGTCTTTCACCATCGAAGAAAAGAGTTGTTAAGATACTACAAGATGTTAGTGGTATTGTCAAACCATCAAGGATGACACTTCTTCTTGGACCTCCGGGATCAGGAAAAACAACATTGCTAAAAGCATTTTCTGGCAAACTAGATAGGGATCTTAGGGAAGCAAGTTTGATCACAGATTATGTCCTCAAGTTATTGGCTTTCATTGGTATAAGTCAGATGGCACTCTCCCTCTTCCGTTTCATTGCAGCGCTTGGAAGATCAGATGTGGTTGCTAACACAATTGGTTCATTTACACTGCTATTGGCCCCCAGCAACACCTCCACTCAGCCTACTCTCGGAAAAGCCCTTCTCAGAGAAAGAGGTCTGTACACTGAAGAATACTGGTATTGGATTTGTGTTGGAGCGCTTTTTGGGTTTTCTTTTCTTTTCAATGGGCTGTTCATTGCAGCACTCACCTACTTGAACCCCCTTGGCGACAACAAAGGTCTAATTCTGGAGGATGATTCTGAAACCAAGAGGAGGCCACAATTGACAGAAGGTATTGATATGCAAGTGAGAAATGCAGAAGGAACTTCAAATGGACAAGCCAAGCGAGGAATGGTGTTGCCCTTCCAGCCCCTTTCACTCGCTTTCAGCCATGTGAACTACTATGTGGATATGCCTGCGGTGGCCATTGAGACGATTTATGTTGCTATACAAACATTCATTTATTCCCTTCTTCTATTTACCATGATCGGCTATGAATTTAAAGTTGAGAAATTTTTGTATTTCTACTACTTTATATTCATGTGTTTTACATACATGTCAATGTATGGAATGATGGTGGTTGCCCTAACTCCCGGCCATCAAATCGCTGCAATTGTCATGTCCTTCTTCATGAATTTCTGGAATTTGTTCTCCGGTTTCCTCCTCGCTAGACCGGAATGTTGA

Protein Analysis

485

Amino Acids

54.67

Weight (kDa)

9.0

Isoelectric Point (pI)

49.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 115 - 163 2.4e-09 ABC-transporter N-terminal
ABC_tran PF00005 189 - 224 6.8e-06 ABC transporter
PDR_assoc PF08370 281 - 339 4.2e-26 Plant PDR ABC transporter associated
ABC2_membrane PF01061 399 - 482 8.7e-14 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 528
Acc16I TGCGCA 1 cut(s) 285
AccB1I GGYRCC 1 cut(s) 461
AccBSI CCGCTC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 92
AccII CGCG 1 cut(s) 119
AciI CCGC 7 cut(s) 5, 8, 88, 136, 185, 196, 1193
AclWI GGATC 3 cut(s) 303, 634, 684
AcoI YGGCCR 4 cut(s) 18, 186, 1197, 1369
AcsI RAATTY 6 cut(s) 430, 487, 1271, 1286, 1408, 1417
AcuI CTGAAG 2 cut(s) 288, 921
AfaI GTAC 1 cut(s) 896
AfeI AGCGCT 2 cut(s) 777, 933
AfiI CCNNNNNNNGG 5 cut(s) 151, 195, 629, 863, 1047
AflIII ACRYGT 2 cut(s) 126, 1329
AgsI TTSAA 5 cut(s) 364, 499, 958, 991, 1104
AjiI CACGTC 1 cut(s) 127
AluBI AGCT 4 cut(s) 60, 84, 175, 370
AluI AGCT 4 cut(s) 60, 84, 175, 370
Alw21I GWGCWC 3 cut(s) 62, 177, 472
Alw26I GTCTC 1 cut(s) 1199
AlwI GGATC 3 cut(s) 303, 634, 684
Aor51HI AGCGCT 2 cut(s) 777, 933
AoxI GGCC 7 cut(s) 18, 186, 375, 831, 1049, 1197, 1369
ApeKI GCWGC 7 cut(s) 11, 48, 72, 148, 773, 974, 1382
ApoI RAATTY 6 cut(s) 430, 487, 1271, 1286, 1408, 1417
Asp700I GAANNNNTTC 1 cut(s) 1421
AspLEI GCGC 5 cut(s) 121, 157, 286, 778, 934
AspS9I GGNCC 3 cut(s) 375, 617, 832
AsuC2I CCSGG 2 cut(s) 624, 1368
AsuHPI GGTGA 2 cut(s) 528, 973
AvaII GGWCC 1 cut(s) 617
BalI TGGCCA 1 cut(s) 1199
BanI GGYRCC 1 cut(s) 461
BanII GRGCYC 2 cut(s) 62, 177
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 3 cut(s) 62, 177, 472
BbvI GCAGC 7 cut(s) 23, 60, 84, 160, 785, 986, 1369
BccI CCATC 7 cut(s) 310, 437, 547, 601, 739, 1342, 1380
BclI TGATCA 1 cut(s) 696
BcnI CCSGG 2 cut(s) 624, 1368
BcoDI GTCTC 1 cut(s) 1199
BfaI CTAG 2 cut(s) 668, 1443
BfoI RGCGCY 3 cut(s) 158, 779, 935
Bme1390I CCNGG 2 cut(s) 624, 1368
Bme18I GGWCC 1 cut(s) 617
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 3 cut(s) 375, 617, 832
BmiI GGNNCC 2 cut(s) 463, 834
BmrFI CCNGG 2 cut(s) 624, 1368
BmsI GCATC 3 cut(s) 295, 405, 439
BoxI GACNNNNGTC 1 cut(s) 98
BpiI GAAGAC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 1043
Bpu10I CCTNAGC 1 cut(s) 371
BpuEI CTTGAG 3 cut(s) 161, 314, 698
BpuMI CCSGG 2 cut(s) 624, 1368
Bsa29I ATCGAT 1 cut(s) 399
BsaBI GATNNNNATC 1 cut(s) 675
BsaJI CCNNGG 1 cut(s) 997
BsaWI WCCGGW 2 cut(s) 1427, 1447
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 5 cut(s) 151, 195, 629, 863, 1047
Bse118I RCCGGY 1 cut(s) 151
Bse1I ACTGG 1 cut(s) 914
Bse3DI GCAATG 5 cut(s) 21, 256, 641, 768, 969
Bse8I GATNNNNATC 1 cut(s) 675
BseCI ATCGAT 1 cut(s) 399
BseDI CCNNGG 1 cut(s) 997
BseGI GGATG 3 cut(s) 222, 606, 1033
BseJI GATNNNNATC 1 cut(s) 675
BseLI CCNNNNNNNGG 5 cut(s) 151, 195, 629, 863, 1047
BseMI GCAATG 5 cut(s) 21, 256, 641, 768, 969
BseMII CTCAG 3 cut(s) 385, 866, 892
BseNI ACTGG 1 cut(s) 914
BseRI GAGGAG 9 cut(s) 70, 94, 179, 182, 185, 235, 356, 1060, 1427
BseXI GCAGC 7 cut(s) 23, 60, 84, 160, 785, 986, 1369
BseYI CCCAGC 2 cut(s) 517, 836
Bsh1236I CGCG 1 cut(s) 119
Bsh1285I CGRYCG 1 cut(s) 92
BshFI GGCC 7 cut(s) 20, 188, 377, 833, 1051, 1199, 1371
BshNI GGYRCC 1 cut(s) 461
BshVI ATCGAT 1 cut(s) 399
BsiEI CGRYCG 1 cut(s) 92
BsiHKAI GWGCWC 3 cut(s) 62, 177, 472
BslFI GGGAC 2 cut(s) 525, 534
BslI CCNNNNNNNGG 5 cut(s) 151, 195, 629, 863, 1047
BsmAI GTCTC 1 cut(s) 1199
BsmBI CGTCTC 1 cut(s) 1199
BsmFI GGGAC 2 cut(s) 525, 534
BsnI GGCC 7 cut(s) 20, 188, 377, 833, 1051, 1199, 1371
Bsp1286I GDGCHC 3 cut(s) 62, 177, 472
Bsp1407I TGTACA 1 cut(s) 894
Bsp143I GATC 6 cut(s) 295, 626, 676, 696, 785, 1260
BspACI CCGC 7 cut(s) 5, 8, 88, 136, 185, 196, 1193
BspANI GGCC 7 cut(s) 20, 188, 377, 833, 1051, 1199, 1371
BspCNI CTCAG 3 cut(s) 384, 865, 891
BspDI ATCGAT 1 cut(s) 399
BspFNI CGCG 1 cut(s) 119
BspHI TCATGA 1 cut(s) 1404
BspLI GGNNCC 2 cut(s) 463, 834
BspPI GGATC 3 cut(s) 303, 634, 684
BspT107I GGYRCC 1 cut(s) 461
BsrBI CCGCTC 1 cut(s) 196
BsrDI GCAATG 5 cut(s) 21, 256, 641, 768, 969
BsrFI RCCGGY 1 cut(s) 151
BsrGI TGTACA 1 cut(s) 894
BsrI ACTGG 1 cut(s) 914
BssAI RCCGGY 1 cut(s) 151
BssECI CCNNGG 1 cut(s) 997
BssMI GATC 6 cut(s) 295, 626, 676, 696, 785, 1260
BssT1I CCWWGG 1 cut(s) 997
Bst6I CTCTTC 1 cut(s) 764
BstAUI TGTACA 1 cut(s) 894
BstC8I GCNNGC 4 cut(s) 62, 86, 153, 1191
BstDEI CTNAG 4 cut(s) 371, 680, 852, 878
BstF5I GGATG 3 cut(s) 222, 606, 1033
BstFNI CGCG 1 cut(s) 119
BstH2I RGCGCY 3 cut(s) 158, 779, 935
BstHHI GCGC 5 cut(s) 121, 157, 286, 778, 934
BstKTI GATC 6 cut(s) 298, 629, 679, 699, 788, 1263
BstMAI GTCTC 1 cut(s) 1199
BstMBI GATC 6 cut(s) 295, 626, 676, 696, 785, 1260
BstMCI CGRYCG 1 cut(s) 92
BstMWI GCNNNNNNNGC 6 cut(s) 11, 17, 57, 81, 154, 1141
BstNSI RCATGY 2 cut(s) 232, 1333
BstPAI GACNNNNGTC 1 cut(s) 98
BstSCI CCNGG 2 cut(s) 622, 1366
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 7 cut(s) 23, 60, 84, 160, 785, 986, 1369
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 676
BstXI CCANNNNNNTGG 1 cut(s) 483
BstYI RGATCY 1 cut(s) 676
Bsu15I ATCGAT 1 cut(s) 399
BsuRI GGCC 7 cut(s) 20, 188, 377, 833, 1051, 1199, 1371
BsuTUI ATCGAT 1 cut(s) 399
BtrI CACGTC 1 cut(s) 127
BtsCI GGATG 3 cut(s) 222, 606, 1033
BtsI GCAGTG 1 cut(s) 821
BtsIMutI CAGTG 2 cut(s) 821, 897
Cac8I GCNNGC 4 cut(s) 62, 86, 153, 1191
CciI TCATGA 1 cut(s) 1404
CfoI GCGC 5 cut(s) 121, 157, 286, 778, 934
Cfr10I RCCGGY 1 cut(s) 151
Cfr13I GGNCC 3 cut(s) 375, 617, 832
ClaI ATCGAT 1 cut(s) 399
CseI GACGC 1 cut(s) 130
Csp6I GTAC 1 cut(s) 895
CviAII CATG 7 cut(s) 229, 1166, 1258, 1315, 1330, 1393, 1405
CviQI GTAC 1 cut(s) 895
DdeI CTNAG 4 cut(s) 371, 680, 852, 878
DpnI GATC 6 cut(s) 297, 628, 678, 698, 787, 1262
DpnII GATC 6 cut(s) 295, 626, 676, 696, 785, 1260
DraI TTTAAA 1 cut(s) 1276
DrdI GACNNNNNNGTC 1 cut(s) 528
DseDI GACNNNNNNGTC 1 cut(s) 528
EaeI YGGCCR 4 cut(s) 18, 186, 1197, 1369
Eam1104I CTCTTC 1 cut(s) 764
EarI CTCTTC 1 cut(s) 764
Ecl136II GAGCTC 2 cut(s) 60, 175
Eco130I CCWWGG 1 cut(s) 997
Eco24I GRGCYC 2 cut(s) 62, 177
Eco47I GGWCC 1 cut(s) 617
Eco47III AGCGCT 2 cut(s) 777, 933
Eco53kI GAGCTC 2 cut(s) 60, 175
Eco57I CTGAAG 2 cut(s) 288, 921
EcoICRI GAGCTC 2 cut(s) 60, 175
EcoRI GAATTC 1 cut(s) 487
EcoT14I CCWWGG 1 cut(s) 997
EcoT38I GRGCYC 2 cut(s) 62, 177
ErhI CCWWGG 1 cut(s) 997
Esp3I CGTCTC 1 cut(s) 1199
FaeI CATG 7 cut(s) 232, 1169, 1261, 1318, 1333, 1396, 1408
FaqI GGGAC 2 cut(s) 525, 534
FatI CATG 7 cut(s) 228, 1165, 1257, 1314, 1329, 1392, 1404
FbaI TGATCA 1 cut(s) 696
FblI GTMKAC 1 cut(s) 92
FokI GGATG 3 cut(s) 229, 613, 1040
FriOI GRGCYC 2 cut(s) 62, 177
FspBI CTAG 2 cut(s) 668, 1443
FspI TGCGCA 1 cut(s) 285
GlaI GCGC 5 cut(s) 120, 156, 285, 777, 933
GsaI CCCAGC 2 cut(s) 521, 840
GsuI CTGGAG 1 cut(s) 1043
HaeII RGCGCY 3 cut(s) 158, 779, 935
HaeIII GGCC 7 cut(s) 20, 188, 377, 833, 1051, 1199, 1371
HgaI GACGC 1 cut(s) 130
HhaI GCGC 5 cut(s) 121, 157, 286, 778, 934
Hin1II CATG 7 cut(s) 232, 1169, 1261, 1318, 1333, 1396, 1408
Hin6I GCGC 5 cut(s) 119, 155, 284, 776, 932
HinP1I GCGC 5 cut(s) 119, 155, 284, 776, 932
HincII GTYRAC 2 cut(s) 93, 277
HindII GTYRAC 2 cut(s) 93, 277
HinfI GANTC 2 cut(s) 423, 1031
HphI GGTGA 2 cut(s) 528, 973
Hpy166II GTNNAC 4 cut(s) 93, 277, 897, 1171
Hpy188I TCNGA 6 cut(s) 104, 744, 790, 866, 881, 1036
Hpy188III TCNNGA 7 cut(s) 299, 361, 427, 630, 1022, 1405, 1414
Hpy8I GTNNAC 4 cut(s) 93, 277, 897, 1171
Hpy99I CGWCG 1 cut(s) 97
HpyAV CCTTC 7 cut(s) 249, 884, 1058, 1088, 1147, 1253, 1408
HpyCH4IV ACGT 2 cut(s) 126, 279
HpyCH4V TGCA 5 cut(s) 773, 974, 1078, 1091, 1385
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 17, 57, 81, 154, 1141
HpyF3I CTNAG 4 cut(s) 371, 680, 852, 878
HpySE526I ACGT 2 cut(s) 126, 279
Hsp92II CATG 7 cut(s) 232, 1169, 1261, 1318, 1333, 1396, 1408
HspAI GCGC 5 cut(s) 119, 155, 284, 776, 932
KroI GCCGGC 1 cut(s) 151
KroNI GCCGGC 1 cut(s) 153
Ksp22I TGATCA 1 cut(s) 696
Kzo9I GATC 6 cut(s) 295, 626, 676, 696, 785, 1260
LmnI GCTCC 5 cut(s) 57, 81, 138, 172, 929
Lsp1109I GCAGC 7 cut(s) 23, 60, 84, 160, 785, 986, 1369
LweI GCATC 3 cut(s) 295, 405, 439
MaeI CTAG 2 cut(s) 668, 1443
MaeII ACGT 2 cut(s) 126, 279
MalI GATC 6 cut(s) 297, 628, 678, 698, 787, 1262
MbiI CCGCTC 1 cut(s) 196
MboI GATC 6 cut(s) 295, 626, 676, 696, 785, 1260
MfeI CAATTG 3 cut(s) 807, 1055, 1386
MflI RGATCY 1 cut(s) 676
MhlI GDGCHC 3 cut(s) 62, 177, 472
MlsI TGGCCA 1 cut(s) 1199
MluNI TGGCCA 1 cut(s) 1199
MmeI TCCRAC 2 cut(s) 500, 907
Mox20I TGGCCA 1 cut(s) 1199
MroNI GCCGGC 1 cut(s) 151
MroXI GAANNNNTTC 1 cut(s) 1421
MscI TGGCCA 1 cut(s) 1199
MseI TTAA 3 cut(s) 32, 558, 1275
MslI CAYNNNNRTG 1 cut(s) 1334
Msp20I TGGCCA 1 cut(s) 1199
MspA1I CMGCKG 1 cut(s) 136
MspR9I CCNGG 2 cut(s) 624, 1368
MunI CAATTG 3 cut(s) 807, 1055, 1386
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 6 cut(s) 11, 17, 57, 81, 154, 1141
NaeI GCCGGC 1 cut(s) 153
NciI CCSGG 2 cut(s) 624, 1368
NdeII GATC 6 cut(s) 295, 626, 676, 696, 785, 1260
NgoMIV GCCGGC 1 cut(s) 151
NlaIII CATG 7 cut(s) 232, 1169, 1261, 1318, 1333, 1396, 1408
NlaIV GGNNCC 2 cut(s) 463, 834
NsbI TGCGCA 1 cut(s) 285
NspI RCATGY 2 cut(s) 232, 1333
PagI TCATGA 1 cut(s) 1404
PciI ACATGT 1 cut(s) 1329
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PdiI GCCGGC 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 1421
PfeI GAWTC 2 cut(s) 423, 1031
PfoI TCCNGGA 1 cut(s) 622
PscI ACATGT 1 cut(s) 1329
PshAI GACNNNNGTC 1 cut(s) 98
Psp124BI GAGCTC 2 cut(s) 62, 177
PspFI CCCAGC 2 cut(s) 517, 836
PspN4I GGNNCC 2 cut(s) 463, 834
PspPI GGNCC 3 cut(s) 375, 617, 832
PsuI RGATCY 1 cut(s) 676
RsaI GTAC 1 cut(s) 896
RsaNI GTAC 1 cut(s) 895
RseI CAYNNNNRTG 1 cut(s) 1334
SacI GAGCTC 2 cut(s) 62, 177
SalI GTCGAC 1 cut(s) 91
SaqAI TTAA 3 cut(s) 32, 558, 1275
Sau3AI GATC 6 cut(s) 295, 626, 676, 696, 785, 1260
Sau96I GGNCC 3 cut(s) 375, 617, 832
ScrFI CCNGG 2 cut(s) 624, 1368
SduI GDGCHC 3 cut(s) 62, 177, 472
SfaNI GCATC 3 cut(s) 295, 405, 439
SinI GGWCC 1 cut(s) 617
SmiMI CAYNNNNRTG 1 cut(s) 1334
SmlI CTYRAG 3 cut(s) 176, 329, 713
SmoI CTYRAG 3 cut(s) 176, 329, 713
SsiI CCGC 7 cut(s) 5, 8, 88, 136, 185, 196, 1193
SspMI CTAG 2 cut(s) 668, 1443
SstI GAGCTC 2 cut(s) 62, 177
StyD4I CCNGG 2 cut(s) 622, 1366
StyI CCWWGG 1 cut(s) 997
TaiI ACGT 2 cut(s) 129, 282
TaqI TCGA 5 cut(s) 44, 68, 92, 399, 542
TatI WGTACW 1 cut(s) 894
TauI GCSGC 3 cut(s) 8, 11, 188
TfiI GAWTC 2 cut(s) 423, 1031
Tru1I TTAA 3 cut(s) 32, 558, 1275
Tru9I TTAA 3 cut(s) 32, 558, 1275
TscAI CASTG 2 cut(s) 828, 904
TseI GCWGC 7 cut(s) 11, 48, 72, 148, 773, 974, 1382
TspGWI ACGGA 1 cut(s) 752
TspRI CASTG 2 cut(s) 828, 904
VpaK11BI GGWCC 1 cut(s) 617
XapI RAATTY 6 cut(s) 430, 487, 1271, 1286, 1408, 1417
XceI RCATGY 2 cut(s) 232, 1333
XcmI CCANNNNNNNNNTGG 1 cut(s) 1123
XmiI GTMKAC 1 cut(s) 92
XmnI GAANNNNTTC 1 cut(s) 1421
XspI CTAG 2 cut(s) 668, 1443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.