Rh5BG371800

Pleiotropic drug resistance protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003940
Physical Location & Seq
Forward (+)
58407228 .. 58416944
9717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG371800.1

Sequence Viewer

Length: 1677 bp
ATGGCGGCTGCAATGGCCGGAGAAGACCTGATGAGGCAGTCGAGTAGCCGGAGGAGCTGGCGGTCGACGAGTGTGCGGGAAATGTGGAACGCGCCGGACGTGTTTCAGAGGAGCGGGAGGCAGCAGGCGGTGGATGAGGAGGAGGAGCTCAAGTGGGCCGCCATAGAGCGGTTGCCGACTTACGATAGGATGAGGAGGGGGATGTTGACTCAGGTGATGAGCAATGGTAGGATTATGCACGACACAGTTGATGTGGCCAATCTTGGCGCTCAGGATAAGAAGCAGTTGATGGAGAGCATACTCAAGGTTGTTGAGGACGATAACGAGAAGTTCTTGCAGAGACTCAGGGCCAGAAACGATAGGGTTGGTATTGAGGTTCCGAAAGTGGAGGTTCGATTCCAGAGCTTAGAGATTGAGGGAGATGCATATGTTGGCACAAGAGCTCTTCCAACTCTACTCAACTCTACTTTGAACGCACTAGAGGCATTAATTGGATTGGTTGGACTCTCACCGTCGAAGAAAAGAGTTGTTAAGATACTCCAAGATGTGAGCGGTATTGTGAAACCATCAAGGGTTACACTTCTTCTTGGACCTCCTTCTTCAGGAAAAACAACACTGCTAAAAGCTCTTGCTGGCAAACTCGATAAAGATCTAAGGTTCTTGGCTTTCTTCGGTATACATCAGATGGCACTCTCCCTCTTCCGTTTCATTGCTGCTCTTGGGAGATCAGAGGTTGTTTCAGGCACAATTGGTTCCTTTACTTTGCTAATGGTGTTTGTCCTAGGAGGTTTCATTGTTGCTAAAGGTATTGACATGCAAGTGAGAAATGCTCAAGGAGGTCAAGCCAAAAGAGGAATGGTCTTGCCTTTCCAGCCCCTTTCACTTGCTTTCAACCATGTGAATTACTATGTGGATATGCCTGCTAAAGCTATAGCAACAATGTTCTTCCAAGAGATGGAAACAAAACCAAAGGAGCTGTTGGAGCTTGCATTGACAAGTCACGCCCTTGAAAAGCCCGAGTTATTTGAAGCTTGCATCGCTAACATTGAGGAGAAGATTATGGGAAAGGATAGAACGCCGAGATGTTATGATGAGCTGGAGCGTAAGAAAAATTACAACAGTGATCAAAGACTCACGCGTATTATTAGAGTATTGGAGCAAGGTAGTAAAACACAATTGCAAAAGAAATCAAAACATCAAGTAATAGGATCAAATGACCCCCTTTCCACTCCCAGCAAGGAAGTTTGGAATGAGAGTGAGAACCCTGTTGATATATATAACCATATGGTTTTCATTAAGGACATATGTTGGTGGAGAAAATTGAGGTTTATTGTGGAGAATGACCGAGCATGGATTAGAGATGAGTTTTCCATACTTCATAAGATAATAATCAGGGATTTGATTAGCCAGATCTCCCGATTTGAGCGGGTAAACAAGAAGAATGTTCGCTTGACTTTGGATAATATAGAGATGGTTGACAACTGTACCAGGATTCTACCTCTTGAAGGCCCAGACATGCCAACTTATAGGATGCAAGTTCGAGATTTACTACCCACATTACTTGGATGTAGTCAAGATCAAGAATTGAATTTATCAACTTGCACTTATCTGGAGCAATGTCACTTTCGAGACATGTTTGACTATGACTTGCCCATATTCGAGTTTGAACAGTTGTGA

Protein Analysis

558

Amino Acids

63.88

Weight (kDa)

8.5

Isoelectric Point (pI)

46.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 106 - 154 7.5e-08 ABC-transporter N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 955
AccBSI CCGCTC 4 cut(s) 114, 169, 552, 1426
AccI GTMKAC 2 cut(s) 65, 676
AccII CGCG 2 cut(s) 92, 1138
AciI CCGC 9 cut(s) 5, 61, 76, 114, 128, 159, 169, 552, 1426
AclWI GGATC 1 cut(s) 1216
AcoI YGGCCR 2 cut(s) 15, 255
AcsI RAATTY 1 cut(s) 1588
AcuI CTGAAG 1 cut(s) 585
AfaI GTAC 1 cut(s) 1486
AfiI CCNNNNNNNGG 4 cut(s) 168, 602, 955, 1505
AflIII ACRYGT 3 cut(s) 99, 1136, 1632
AgsI TTSAA 7 cut(s) 472, 892, 1010, 1028, 1505, 1588, 1667
AjiI CACGTC 1 cut(s) 100
AjnI CCWGG 1 cut(s) 1487
Alw21I GWGCWC 2 cut(s) 150, 445
Alw26I GTCTC 2 cut(s) 334, 1623
AlwI GGATC 1 cut(s) 1216
Ama87I CYCGRG 1 cut(s) 1016
AoxI GGCC 5 cut(s) 15, 156, 255, 348, 1507
ApeKI GCWGC 3 cut(s) 8, 121, 713
ApoI RAATTY 1 cut(s) 1588
AseI ATTAAT 1 cut(s) 488
AspA2I CCTAGG 1 cut(s) 781
AspLEI GCGC 2 cut(s) 94, 269
AspS9I GGNCC 4 cut(s) 156, 348, 590, 1508
AsuHPI GGTGA 2 cut(s) 226, 501
AvaI CYCGRG 1 cut(s) 1016
AvaII GGWCC 1 cut(s) 590
AvrII CCTAGG 1 cut(s) 781
BaeI ACNNNNGTAYC 2 cut(s) 1468, 1501
BalI TGGCCA 1 cut(s) 257
BanII GRGCYC 2 cut(s) 150, 445
BbsI GAAGAC 1 cut(s) 30
Bbv12I GWGCWC 2 cut(s) 150, 445
BbvI GCAGC 2 cut(s) 133, 700
BccI CCATC 5 cut(s) 283, 574, 679, 949, 1465
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BciT130I CCWGG 1 cut(s) 1489
BclI TGATCA 1 cut(s) 1123
BcoDI GTCTC 2 cut(s) 334, 1623
BfaI CTAG 2 cut(s) 479, 782
BfmI CTRYAG 1 cut(s) 930
BfoI RGCGCY 1 cut(s) 270
BglII AGATCT 2 cut(s) 649, 1410
BisI GCNGC 5 cut(s) 6, 9, 122, 159, 714
BlnI CCTAGG 1 cut(s) 781
BlsI GCNGC 5 cut(s) 7, 10, 123, 160, 715
Bme1390I CCNGG 1 cut(s) 1489
Bme18I GGWCC 1 cut(s) 590
BmeT110I CYCGRG 1 cut(s) 1016
BmgBI CACGTC 1 cut(s) 100
BmgT120I GGNCC 4 cut(s) 156, 348, 590, 1508
BmiI GGNNCC 2 cut(s) 378, 754
BmrFI CCNGG 1 cut(s) 1489
BmsI GCATC 3 cut(s) 412, 1044, 1521
BpiI GAAGAC 1 cut(s) 30
BplI GAGNNNNNCTC 2 cut(s) 814, 846
BpmI CTGGAG 2 cut(s) 1118, 1631
Bpu10I CCTNAGC 1 cut(s) 270
BpuEI CTTGAG 3 cut(s) 134, 287, 816
BsaBI GATNNNNATC 2 cut(s) 648, 1388
BsaJI CCNNGG 1 cut(s) 781
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 4 cut(s) 168, 602, 955, 1505
Bse3DI GCAATG 4 cut(s) 18, 229, 708, 1622
Bse8I GATNNNNATC 2 cut(s) 648, 1388
BseBI CCWGG 1 cut(s) 1489
BseDI CCNNGG 1 cut(s) 781
BseGI GGATG 5 cut(s) 139, 195, 207, 1536, 1571
BseJI GATNNNNATC 2 cut(s) 648, 1388
BseLI CCNNNNNNNGG 4 cut(s) 168, 602, 955, 1505
BseMI GCAATG 4 cut(s) 18, 229, 708, 1622
BseMII CTCAG 3 cut(s) 224, 284, 358
BseRI GAGGAG 7 cut(s) 67, 124, 152, 155, 158, 208, 1064
BseXI GCAGC 2 cut(s) 133, 700
BseYI CCCAGC 1 cut(s) 1232
Bsh1236I CGCG 2 cut(s) 92, 1138
Bsh1285I CGRYCG 1 cut(s) 65
BshFI GGCC 5 cut(s) 17, 158, 257, 350, 1509
BsiEI CGRYCG 1 cut(s) 65
BsiHKAI GWGCWC 2 cut(s) 150, 445
BsiHKCI CYCGRG 1 cut(s) 1016
BsiSI CCGG 3 cut(s) 18, 49, 95
BslI CCNNNNNNNGG 4 cut(s) 168, 602, 955, 1505
BsmAI GTCTC 2 cut(s) 334, 1623
BsnI GGCC 5 cut(s) 17, 158, 257, 350, 1509
BsoBI CYCGRG 1 cut(s) 1016
Bsp1286I GDGCHC 2 cut(s) 150, 445
Bsp143I GATC 6 cut(s) 649, 725, 1123, 1208, 1410, 1576
BspACI CCGC 9 cut(s) 5, 61, 76, 114, 128, 159, 169, 552, 1426
BspANI GGCC 5 cut(s) 17, 158, 257, 350, 1509
BspCNI CTCAG 3 cut(s) 223, 283, 357
BspFNI CGCG 2 cut(s) 92, 1138
BspLI GGNNCC 2 cut(s) 378, 754
BspPI GGATC 1 cut(s) 1216
BspQI GCTCTTC 1 cut(s) 450
BsrBI CCGCTC 4 cut(s) 114, 169, 552, 1426
BsrDI GCAATG 4 cut(s) 18, 229, 708, 1622
BssECI CCNNGG 1 cut(s) 781
BssMI GATC 6 cut(s) 649, 725, 1123, 1208, 1410, 1576
BssNAI GTATAC 1 cut(s) 677
BssT1I CCWWGG 1 cut(s) 781
Bst1107I GTATAC 1 cut(s) 677
Bst2UI CCWGG 1 cut(s) 1489
Bst4CI ACNGT 5 cut(s) 247, 513, 1121, 1484, 1671
Bst6I CTCTTC 2 cut(s) 450, 704
BstC8I GCNNGC 6 cut(s) 59, 126, 634, 921, 987, 1033
BstDEI CTNAG 5 cut(s) 210, 270, 344, 406, 653
BstENI CCTNNNNNAGG 2 cut(s) 600, 1503
BstF5I GGATG 5 cut(s) 139, 195, 207, 1536, 1571
BstFNI CGCG 2 cut(s) 92, 1138
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 2 cut(s) 94, 269
BstKTI GATC 6 cut(s) 652, 728, 1126, 1211, 1413, 1579
BstMAI GTCTC 2 cut(s) 334, 1623
BstMBI GATC 6 cut(s) 649, 725, 1123, 1208, 1410, 1576
BstMCI CGRYCG 1 cut(s) 65
BstMWI GCNNNNNNNGC 6 cut(s) 14, 54, 482, 871, 982, 1037
BstNI CCWGG 1 cut(s) 1489
BstNSI RCATGY 3 cut(s) 817, 1519, 1636
BstSCI CCNGG 1 cut(s) 1487
BstSFI CTRYAG 1 cut(s) 930
BstUI CGCG 2 cut(s) 92, 1138
BstV1I GCAGC 2 cut(s) 133, 700
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 2 cut(s) 649, 1410
BstYI RGATCY 2 cut(s) 649, 1410
BstZ17I GTATAC 1 cut(s) 677
BsuRI GGCC 5 cut(s) 17, 158, 257, 350, 1509
BtgZI GCGATG 1 cut(s) 1021
BtrI CACGTC 1 cut(s) 100
BtsCI GGATG 5 cut(s) 139, 195, 207, 1536, 1571
BtsI GCAGTG 1 cut(s) 614
BtsIMutI CAGTG 2 cut(s) 614, 1126
Cac8I GCNNGC 6 cut(s) 59, 126, 634, 921, 987, 1033
CfoI GCGC 2 cut(s) 94, 269
Cfr13I GGNCC 4 cut(s) 156, 348, 590, 1508
Csp6I GTAC 1 cut(s) 1485
CspCI CAANNNNNGTGG 2 cut(s) 1543, 1578
CviAII CATG 5 cut(s) 814, 896, 1350, 1516, 1633
CviQI GTAC 1 cut(s) 1485
DdeI CTNAG 5 cut(s) 210, 270, 344, 406, 653
DpnI GATC 6 cut(s) 651, 727, 1125, 1210, 1412, 1578
DpnII GATC 6 cut(s) 649, 725, 1123, 1208, 1410, 1576
EaeI YGGCCR 2 cut(s) 15, 255
Eam1104I CTCTTC 2 cut(s) 450, 704
EarI CTCTTC 2 cut(s) 450, 704
Ecl136II GAGCTC 2 cut(s) 148, 443
Eco130I CCWWGG 1 cut(s) 781
Eco24I GRGCYC 2 cut(s) 150, 445
Eco47I GGWCC 1 cut(s) 590
Eco53kI GAGCTC 2 cut(s) 148, 443
Eco57I CTGAAG 1 cut(s) 585
Eco88I CYCGRG 1 cut(s) 1016
EcoICRI GAGCTC 2 cut(s) 148, 443
EcoNI CCTNNNNNAGG 2 cut(s) 600, 1503
EcoRII CCWGG 1 cut(s) 1487
EcoT14I CCWWGG 1 cut(s) 781
EcoT22I ATGCAT 1 cut(s) 427
EcoT38I GRGCYC 2 cut(s) 150, 445
ErhI CCWWGG 1 cut(s) 781
FaeI CATG 5 cut(s) 817, 899, 1353, 1519, 1636
FatI CATG 5 cut(s) 813, 895, 1349, 1515, 1632
FauI CCCGC 3 cut(s) 69, 107, 1419
FauNDI CATATG 3 cut(s) 427, 1284, 1304
FbaI TGATCA 1 cut(s) 1123
FblI GTMKAC 2 cut(s) 65, 676
Fnu4HI GCNGC 5 cut(s) 6, 9, 122, 159, 714
FokI GGATG 5 cut(s) 146, 202, 214, 1543, 1578
FriOI GRGCYC 2 cut(s) 150, 445
Fsp4HI GCNGC 5 cut(s) 6, 9, 122, 159, 714
FspBI CTAG 2 cut(s) 479, 782
GlaI GCGC 2 cut(s) 93, 268
GluI GCNGC 5 cut(s) 6, 9, 122, 159, 714
GsaI CCCAGC 1 cut(s) 1236
GsuI CTGGAG 2 cut(s) 1118, 1631
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 5 cut(s) 17, 158, 257, 350, 1509
HapII CCGG 3 cut(s) 18, 49, 95
HhaI GCGC 2 cut(s) 94, 269
Hin1II CATG 5 cut(s) 817, 899, 1353, 1519, 1636
Hin6I GCGC 2 cut(s) 92, 267
HinP1I GCGC 2 cut(s) 92, 267
HincII GTYRAC 3 cut(s) 66, 207, 1477
HindII GTYRAC 3 cut(s) 66, 207, 1477
HindIII AAGCTT 1 cut(s) 1029
HinfI GANTC 6 cut(s) 208, 342, 396, 504, 1131, 1492
HpaII CCGG 3 cut(s) 18, 49, 95
HphI GGTGA 2 cut(s) 226, 501
Hpy166II GTNNAC 5 cut(s) 66, 207, 677, 1432, 1477
Hpy188I TCNGA 4 cut(s) 108, 381, 684, 730
Hpy8I GTNNAC 5 cut(s) 66, 207, 677, 1432, 1477
Hpy99I CGWCG 2 cut(s) 70, 517
HpyAV CCTTC 2 cut(s) 606, 1499
HpyCH4III ACNGT 5 cut(s) 247, 513, 1121, 1484, 1671
HpyCH4IV ACGT 1 cut(s) 99
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 54, 482, 871, 982, 1037
HpyF3I CTNAG 5 cut(s) 210, 270, 344, 406, 653
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 5 cut(s) 817, 899, 1353, 1519, 1636
HspAI GCGC 2 cut(s) 92, 267
Ksp22I TGATCA 1 cut(s) 1123
Kzo9I GATC 6 cut(s) 649, 725, 1123, 1208, 1410, 1576
LguI GCTCTTC 1 cut(s) 450
LmnI GCTCC 8 cut(s) 54, 111, 145, 973, 982, 1099, 1156, 1612
Lsp1109I GCAGC 2 cut(s) 133, 700
LweI GCATC 3 cut(s) 412, 1044, 1521
MaeI CTAG 2 cut(s) 479, 782
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 3 cut(s) 574, 998, 1619
MalI GATC 6 cut(s) 651, 727, 1125, 1210, 1412, 1578
MbiI CCGCTC 4 cut(s) 114, 169, 552, 1426
MboI GATC 6 cut(s) 649, 725, 1123, 1208, 1410, 1576
MfeI CAATTG 2 cut(s) 747, 1175
MflI RGATCY 2 cut(s) 649, 1410
MhlI GDGCHC 2 cut(s) 150, 445
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 8 cut(s) 489, 747, 901, 1111, 1175, 1319, 1583, 1588
MluI ACGCGT 1 cut(s) 1136
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 4 cut(s) 202, 336, 498, 1125
MmeI TCCRAC 3 cut(s) 473, 481, 960
Mox20I TGGCCA 1 cut(s) 257
Mph1103I ATGCAT 1 cut(s) 427
MscI TGGCCA 1 cut(s) 257
MseI TTAA 3 cut(s) 488, 531, 1296
MslI CAYNNNNRTG 1 cut(s) 818
Msp20I TGGCCA 1 cut(s) 257
MspI CCGG 3 cut(s) 18, 49, 95
MspR9I CCNGG 1 cut(s) 1489
MunI CAATTG 2 cut(s) 747, 1175
MvaI CCWGG 1 cut(s) 1489
MvnI CGCG 2 cut(s) 92, 1138
MwoI GCNNNNNNNGC 6 cut(s) 14, 54, 482, 871, 982, 1037
NdeI CATATG 3 cut(s) 427, 1284, 1304
NdeII GATC 6 cut(s) 649, 725, 1123, 1208, 1410, 1576
NlaIII CATG 5 cut(s) 817, 899, 1353, 1519, 1636
NlaIV GGNNCC 2 cut(s) 378, 754
NmeAIII GCCGAG 1 cut(s) 1104
NmuCI GTSAC 2 cut(s) 998, 1619
NsiI ATGCAT 1 cut(s) 427
NspI RCATGY 3 cut(s) 817, 1519, 1636
PciI ACATGT 1 cut(s) 1632
PciSI GCTCTTC 1 cut(s) 450
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PfeI GAWTC 2 cut(s) 396, 1492
PflMI CCANNNNNTGG 1 cut(s) 955
PkrI GCNGC 5 cut(s) 7, 10, 123, 160, 715
PleI GAGTC 4 cut(s) 202, 336, 498, 1125
PpsI GAGTC 4 cut(s) 202, 336, 498, 1125
PscI ACATGT 1 cut(s) 1632
PshBI ATTAAT 1 cut(s) 488
Psp124BI GAGCTC 2 cut(s) 150, 445
Psp6I CCWGG 1 cut(s) 1487
PspFI CCCAGC 1 cut(s) 1232
PspGI CCWGG 1 cut(s) 1487
PspN4I GGNNCC 2 cut(s) 378, 754
PspPI GGNCC 4 cut(s) 156, 348, 590, 1508
PsuI RGATCY 2 cut(s) 649, 1410
RsaI GTAC 1 cut(s) 1486
RsaNI GTAC 1 cut(s) 1485
RseI CAYNNNNRTG 1 cut(s) 818
SacI GAGCTC 2 cut(s) 150, 445
SalI GTCGAC 1 cut(s) 64
SapI GCTCTTC 1 cut(s) 450
SaqAI TTAA 3 cut(s) 488, 531, 1296
SatI GCNGC 5 cut(s) 6, 9, 122, 159, 714
Sau3AI GATC 6 cut(s) 649, 725, 1123, 1208, 1410, 1576
Sau96I GGNCC 4 cut(s) 156, 348, 590, 1508
SchI GAGTC 4 cut(s) 202, 336, 498, 1125
ScrFI CCNGG 1 cut(s) 1489
SduI GDGCHC 2 cut(s) 150, 445
SfaNI GCATC 3 cut(s) 412, 1044, 1521
SfcI CTRYAG 1 cut(s) 930
SinI GGWCC 1 cut(s) 590
SmiMI CAYNNNNRTG 1 cut(s) 818
SmlI CTYRAG 3 cut(s) 149, 302, 831
SmoI CTYRAG 3 cut(s) 149, 302, 831
Sse9I AATT 8 cut(s) 489, 747, 901, 1111, 1175, 1319, 1583, 1588
SsiI CCGC 9 cut(s) 5, 61, 76, 114, 128, 159, 169, 552, 1426
SspMI CTAG 2 cut(s) 479, 782
SstI GAGCTC 2 cut(s) 150, 445
StyD4I CCNGG 1 cut(s) 1487
StyI CCWWGG 1 cut(s) 781
TaaI ACNGT 5 cut(s) 247, 513, 1121, 1484, 1671
TaiI ACGT 1 cut(s) 102
TaqI TCGA 8 cut(s) 41, 65, 394, 515, 642, 1540, 1627, 1659
TaqII GACCGA 1 cut(s) 1359
TasI AATT 8 cut(s) 489, 747, 901, 1111, 1175, 1319, 1583, 1588
TauI GCSGC 2 cut(s) 8, 161
TfiI GAWTC 2 cut(s) 396, 1492
Tru1I TTAA 3 cut(s) 488, 531, 1296
Tru9I TTAA 3 cut(s) 488, 531, 1296
TscAI CASTG 2 cut(s) 621, 1126
TseFI GTSAC 2 cut(s) 998, 1619
TseI GCWGC 3 cut(s) 8, 121, 713
Tsp45I GTSAC 2 cut(s) 998, 1619
TspDTI ATGAA 4 cut(s) 697, 781, 1282, 1367
TspGWI ACGGA 1 cut(s) 692
TspRI CASTG 2 cut(s) 621, 1126
Van91I CCANNNNNTGG 1 cut(s) 955
VpaK11BI GGWCC 1 cut(s) 590
VspI ATTAAT 1 cut(s) 488
XagI CCTNNNNNAGG 2 cut(s) 600, 1503
XapI RAATTY 1 cut(s) 1588
XceI RCATGY 3 cut(s) 817, 1519, 1636
XcmI CCANNNNNNNNNTGG 1 cut(s) 853
XmaJI CCTAGG 1 cut(s) 781
XmiI GTMKAC 2 cut(s) 65, 676
XspI CTAG 2 cut(s) 479, 782
Zsp2I ATGCAT 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.