Rh5BG371500

Pleiotropic drug resistance protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003940
Physical Location & Seq
Reverse (-)
58355373 .. 58363768
8396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG371500.1

Sequence Viewer

Length: 1053 bp
ATGGCGGCGGCAGCATTGGCCGGAGAAGGTTTGGTGCGGTCTGGAAGTAGCATGAAATGGCGGGCAACAAGCATGAAAGACATGTGGAATGATCAGCCAGACGCATTCCAAAGGAGTGAGAGGAAGGGGGAGGAGGAGGAGGAGCTGAGGTGGGCTGCAATAGAGCGGCTGCCCACTTATGACAGGTTGAAGAAGGGGATGTTGAGGCAGGTCAGGAGCGATGGAAGGATAATGCATGATGAGGTTGATGTTACAAAGCTTAGGGCAGATGATAAGAAGAAGTTGATGGATAGCATACTAAAGGTTGTTGAGGATGATAATGAGAAATTTCTCAGGAAGCTCAGGGACAGGACTGACAGAATGACTCTACTTCTTGGACCTCCTGCCTCAGGAAAAACAACATTTCTAAAGGCACTTTCTGGAAAACTTGATGATGATTTAAGGCCCATAACTGGTTCTAGCCAACCTACAGTTGGAAAGACCCTCCTTAAGGAAAGAGGACTGTTTGTAGACGAATATTGGTATTGGATTTGCATTGGAGCACTCCTTGGATATTCTCTTCTCTTTAACGTTCTTTTCATTGCAGCACTAACATTTTTGAAGCCACTTGGTGATTCTAAAGCGGTAATTTCGGACGACCACTCTGATCTCAATACTAAAAAGCAGTTGACATCCAATTCAGAAGGCACTGATACTCCGGTGAGGAATACCAGGGAGACTACAAGCTCAAGCATGAGTTCTTCAAATACTGAATCTCGAAGAGGAATGGTGTTGCCCTTCCAGCCCCTTTCACTTGCTTTTGACCATGTTAACTACTATGTGGATATGCCTGCTCTGCATAGAGTAACAAAAGGATGCTCTTCACCCCAGGATGCTTCTTGGTTCCCAAATCAAGTTCATCCCAATCCTCTGCATTTCCCAGATAACCATCTAGACAATTTGCTTCATCGGTCTCTAAGTTTTGCTTCCATCGCTGCTGTTGCTGTGGCCAACAGAACTGACATACCACCACTCAGCCTTCCTGTGTTTACCATTGGCAAACAACAAGTGTAG

Protein Analysis

350

Amino Acids

39.26

Weight (kDa)

8.38

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDR_assoc PF08370 153 - 210 1.1e-27 Plant PDR ABC transporter associated
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 199
AccBSI CCGCTC 1 cut(s) 166
AccI GTMKAC 1 cut(s) 510
AciI CCGC 6 cut(s) 5, 8, 37, 61, 166, 623
AclI AACGTT 1 cut(s) 570
AcoI YGGCCR 2 cut(s) 18, 987
AcsI RAATTY 1 cut(s) 326
AdeI CACNNNGTG 1 cut(s) 611
AfiI CCNNNNNNNGG 4 cut(s) 389, 452, 473, 490
AflII CTTAAG 1 cut(s) 488
AflIII ACRYGT 1 cut(s) 81
AgsI TTSAA 3 cut(s) 190, 601, 744
AjnI CCWGG 2 cut(s) 710, 867
AluBI AGCT 4 cut(s) 145, 259, 340, 726
AluI AGCT 4 cut(s) 145, 259, 340, 726
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 2 cut(s) 710, 957
AoxI GGCC 3 cut(s) 18, 443, 987
ApeKI GCWGC 5 cut(s) 11, 155, 169, 584, 974
ApoI RAATTY 1 cut(s) 326
AspS9I GGNCC 2 cut(s) 377, 444
AsuHPI GGTGA 3 cut(s) 623, 712, 855
AvaII GGWCC 1 cut(s) 377
AxyI CCTNAGG 1 cut(s) 388
BaeI ACNNNNGTAYC 2 cut(s) 684, 717
BalI TGGCCA 1 cut(s) 989
Bbv12I GWGCWC 1 cut(s) 544
BbvCI CCTCAGC 1 cut(s) 146
BbvI GCAGC 5 cut(s) 23, 142, 156, 596, 961
BccI CCATC 4 cut(s) 215, 280, 936, 977
BciT130I CCWGG 2 cut(s) 712, 869
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 2 cut(s) 710, 957
BfaI CTAG 2 cut(s) 459, 932
BfmI CTRYAG 1 cut(s) 468
BfrI CTTAAG 1 cut(s) 488
BfuAI ACCTGC 1 cut(s) 199
BisI GCNGC 8 cut(s) 6, 9, 12, 156, 167, 170, 585, 975
BlsI GCNGC 8 cut(s) 7, 10, 13, 157, 168, 171, 586, 976
Bme1390I CCNGG 2 cut(s) 712, 869
Bme18I GGWCC 1 cut(s) 377
BmgT120I GGNCC 2 cut(s) 377, 444
BmiI GGNNCC 1 cut(s) 884
BmrFI CCNGG 2 cut(s) 712, 869
BmsI GCATC 2 cut(s) 845, 862
Bpu10I CCTNAGC 3 cut(s) 146, 260, 341
BpuEI CTTGAG 1 cut(s) 712
BsaBI GATNNNNATC 1 cut(s) 927
BsaI GGTCTC 1 cut(s) 957
BsaJI CCNNGG 3 cut(s) 547, 711, 867
BsaWI WCCGGW 1 cut(s) 697
BsaXI ACNNNNNCTCC 4 cut(s) 134, 164, 679, 709
Bsc4I CCNNNNNNNGG 4 cut(s) 389, 452, 473, 490
Bse1I ACTGG 1 cut(s) 457
Bse21I CCTNAGG 1 cut(s) 388
Bse3DI GCAATG 1 cut(s) 579
Bse8I GATNNNNATC 1 cut(s) 927
BseBI CCWGG 2 cut(s) 712, 869
BseDI CCNNGG 3 cut(s) 547, 711, 867
BseGI GGATG 6 cut(s) 204, 319, 671, 860, 877, 898
BseJI GATNNNNATC 1 cut(s) 927
BseLI CCNNNNNNNGG 4 cut(s) 389, 452, 473, 490
BseMI GCAATG 1 cut(s) 579
BseMII CTCAG 5 cut(s) 137, 346, 355, 402, 1027
BseNI ACTGG 1 cut(s) 457
BseRI GAGGAG 4 cut(s) 146, 149, 152, 155
BseXI GCAGC 5 cut(s) 23, 142, 156, 596, 961
BshFI GGCC 3 cut(s) 20, 445, 989
BsiHKAI GWGCWC 1 cut(s) 544
BsiSI CCGG 2 cut(s) 21, 698
BslFI GGGAC 1 cut(s) 359
BslI CCNNNNNNNGG 4 cut(s) 389, 452, 473, 490
BsmAI GTCTC 2 cut(s) 710, 957
BsmFI GGGAC 1 cut(s) 359
BsmI GAATGC 1 cut(s) 104
BsnI GGCC 3 cut(s) 20, 445, 989
Bso31I GGTCTC 1 cut(s) 957
Bsp1286I GDGCHC 1 cut(s) 544
Bsp143I GATC 2 cut(s) 91, 646
BspACI CCGC 6 cut(s) 5, 8, 37, 61, 166, 623
BspANI GGCC 3 cut(s) 20, 445, 989
BspCNI CTCAG 5 cut(s) 138, 345, 354, 401, 1026
BspLI GGNNCC 1 cut(s) 884
BspMI ACCTGC 1 cut(s) 199
BspQI GCTCTTC 1 cut(s) 865
BspTI CTTAAG 1 cut(s) 488
BspTNI GGTCTC 1 cut(s) 957
BsrBI CCGCTC 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 579
BsrI ACTGG 1 cut(s) 457
BssECI CCNNGG 3 cut(s) 547, 711, 867
BssMI GATC 2 cut(s) 91, 646
BssT1I CCWWGG 1 cut(s) 547
Bst2UI CCWGG 2 cut(s) 712, 869
Bst4CI ACNGT 2 cut(s) 472, 504
Bst6I CTCTTC 3 cut(s) 564, 754, 865
BstAFI CTTAAG 1 cut(s) 488
BstC8I GCNNGC 2 cut(s) 63, 831
BstDEI CTNAG 7 cut(s) 146, 260, 332, 341, 388, 956, 1013
BstENI CCTNNNNNAGG 2 cut(s) 387, 488
BstF5I GGATG 6 cut(s) 204, 319, 671, 860, 877, 898
BstKTI GATC 2 cut(s) 94, 649
BstMAI GTCTC 2 cut(s) 710, 957
BstMBI GATC 2 cut(s) 91, 646
BstMWI GCNNNNNNNGC 6 cut(s) 11, 17, 781, 835, 971, 980
BstNI CCWGG 2 cut(s) 712, 869
BstNSI RCATGY 1 cut(s) 85
BstSCI CCNGG 2 cut(s) 710, 867
BstSFI CTRYAG 1 cut(s) 468
BstV1I GCAGC 5 cut(s) 23, 142, 156, 596, 961
Bsu36I CCTNAGG 1 cut(s) 388
BsuRI GGCC 3 cut(s) 20, 445, 989
BtgZI GCGATG 2 cut(s) 234, 955
BtsCI GGATG 6 cut(s) 204, 319, 671, 860, 877, 898
BtsIMutI CAGTG 1 cut(s) 687
BveI ACCTGC 1 cut(s) 199
Cac8I GCNNGC 2 cut(s) 63, 831
Cfr13I GGNCC 2 cut(s) 377, 444
CseI GACGC 1 cut(s) 110
CviAII CATG 6 cut(s) 52, 73, 82, 236, 733, 806
DdeI CTNAG 7 cut(s) 146, 260, 332, 341, 388, 956, 1013
DpnI GATC 2 cut(s) 93, 648
DpnII GATC 2 cut(s) 91, 646
DraIII CACNNNGTG 1 cut(s) 611
EaeI YGGCCR 2 cut(s) 18, 987
Eam1104I CTCTTC 3 cut(s) 564, 754, 865
EarI CTCTTC 3 cut(s) 564, 754, 865
Eco130I CCWWGG 1 cut(s) 547
Eco31I GGTCTC 1 cut(s) 957
Eco47I GGWCC 1 cut(s) 377
Eco81I CCTNAGG 1 cut(s) 388
EcoNI CCTNNNNNAGG 2 cut(s) 387, 488
EcoRII CCWGG 2 cut(s) 710, 867
EcoT14I CCWWGG 1 cut(s) 547
EcoT22I ATGCAT 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 547
FaeI CATG 6 cut(s) 55, 76, 85, 239, 736, 809
FalI AAGNNNNNCTT 2 cut(s) 949, 981
FaqI GGGAC 1 cut(s) 359
FatI CATG 6 cut(s) 51, 72, 81, 235, 732, 805
FauI CCCGC 1 cut(s) 54
FbaI TGATCA 1 cut(s) 91
FblI GTMKAC 1 cut(s) 510
Fnu4HI GCNGC 8 cut(s) 6, 9, 12, 156, 167, 170, 585, 975
FokI GGATG 6 cut(s) 211, 326, 658, 867, 884, 885
Fsp4HI GCNGC 8 cut(s) 6, 9, 12, 156, 167, 170, 585, 975
FspBI CTAG 2 cut(s) 459, 932
GluI GCNGC 8 cut(s) 6, 9, 12, 156, 167, 170, 585, 975
HaeIII GGCC 3 cut(s) 20, 445, 989
HapII CCGG 2 cut(s) 21, 698
HgaI GACGC 1 cut(s) 110
Hin1II CATG 6 cut(s) 55, 76, 85, 239, 736, 809
HincII GTYRAC 2 cut(s) 669, 811
HindII GTYRAC 2 cut(s) 669, 811
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 3 cut(s) 364, 614, 752
HpaI GTTAAC 1 cut(s) 811
HpaII CCGG 2 cut(s) 21, 698
HphI GGTGA 3 cut(s) 623, 712, 855
Hpy166II GTNNAC 4 cut(s) 511, 669, 811, 1029
Hpy188I TCNGA 3 cut(s) 634, 646, 682
Hpy188III TCNNGA 7 cut(s) 42, 214, 334, 390, 420, 756, 932
Hpy8I GTNNAC 4 cut(s) 511, 669, 811, 1029
HpyAV CCTTC 7 cut(s) 20, 118, 187, 219, 677, 787, 1028
HpyCH4III ACNGT 2 cut(s) 472, 504
HpyCH4IV ACGT 1 cut(s) 570
HpyCH4V TGCA 6 cut(s) 158, 235, 534, 584, 838, 913
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 17, 781, 835, 971, 980
HpyF3I CTNAG 7 cut(s) 146, 260, 332, 341, 388, 956, 1013
HpySE526I ACGT 1 cut(s) 570
Hsp92II CATG 6 cut(s) 55, 76, 85, 239, 736, 809
Ksp22I TGATCA 1 cut(s) 91
KspAI GTTAAC 1 cut(s) 811
Kzo9I GATC 2 cut(s) 91, 646
LguI GCTCTTC 1 cut(s) 865
LmnI GCTCC 3 cut(s) 142, 216, 539
Lsp1109I GCAGC 5 cut(s) 23, 142, 156, 596, 961
LweI GCATC 2 cut(s) 845, 862
MaeI CTAG 2 cut(s) 459, 932
MaeII ACGT 1 cut(s) 570
MaeIII GTNAC 2 cut(s) 250, 844
MalI GATC 2 cut(s) 93, 648
MbiI CCGCTC 1 cut(s) 166
MboI GATC 2 cut(s) 91, 646
MboII GAAGA 6 cut(s) 202, 289, 551, 732, 771, 852
MhlI GDGCHC 1 cut(s) 544
MlsI TGGCCA 1 cut(s) 989
MluCI AATT 4 cut(s) 326, 627, 676, 937
MluNI TGGCCA 1 cut(s) 989
MlyI GAGTC 1 cut(s) 358
MmeI TCCRAC 1 cut(s) 454
Mox20I TGGCCA 1 cut(s) 989
Mph1103I ATGCAT 1 cut(s) 237
MscI TGGCCA 1 cut(s) 989
MseI TTAA 4 cut(s) 440, 489, 567, 810
Msp20I TGGCCA 1 cut(s) 989
MspCI CTTAAG 1 cut(s) 488
MspI CCGG 2 cut(s) 21, 698
MspR9I CCNGG 2 cut(s) 712, 869
Mva1269I GAATGC 1 cut(s) 104
MvaI CCWGG 2 cut(s) 712, 869
MwoI GCNNNNNNNGC 6 cut(s) 11, 17, 781, 835, 971, 980
NdeII GATC 2 cut(s) 91, 646
NlaIII CATG 6 cut(s) 55, 76, 85, 239, 736, 809
NlaIV GGNNCC 1 cut(s) 884
NsiI ATGCAT 1 cut(s) 237
NspI RCATGY 1 cut(s) 85
PciI ACATGT 1 cut(s) 81
PciSI GCTCTTC 1 cut(s) 865
PctI GAATGC 1 cut(s) 104
PfeI GAWTC 2 cut(s) 614, 752
PkrI GCNGC 8 cut(s) 7, 10, 13, 157, 168, 171, 586, 976
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
PscI ACATGT 1 cut(s) 81
Psp1406I AACGTT 1 cut(s) 570
Psp6I CCWGG 2 cut(s) 710, 867
PspGI CCWGG 2 cut(s) 710, 867
PspN4I GGNNCC 1 cut(s) 884
PspPI GGNCC 2 cut(s) 377, 444
SapI GCTCTTC 1 cut(s) 865
SaqAI TTAA 4 cut(s) 440, 489, 567, 810
SatI GCNGC 8 cut(s) 6, 9, 12, 156, 167, 170, 585, 975
Sau3AI GATC 2 cut(s) 91, 646
Sau96I GGNCC 2 cut(s) 377, 444
SchI GAGTC 1 cut(s) 358
ScrFI CCNGG 2 cut(s) 712, 869
SduI GDGCHC 1 cut(s) 544
SfaNI GCATC 2 cut(s) 845, 862
SfcI CTRYAG 1 cut(s) 468
SinI GGWCC 1 cut(s) 377
SmlI CTYRAG 2 cut(s) 488, 727
SmoI CTYRAG 2 cut(s) 488, 727
Sse9I AATT 4 cut(s) 326, 627, 676, 937
SsiI CCGC 6 cut(s) 5, 8, 37, 61, 166, 623
SspI AATATT 1 cut(s) 518
SspMI CTAG 2 cut(s) 459, 932
StyD4I CCNGG 2 cut(s) 710, 867
StyI CCWWGG 1 cut(s) 547
TaaI ACNGT 2 cut(s) 472, 504
TaiI ACGT 1 cut(s) 573
TaqI TCGA 1 cut(s) 757
TaqII GACCGA 1 cut(s) 939
TasI AATT 4 cut(s) 326, 627, 676, 937
TauI GCSGC 3 cut(s) 8, 11, 169
TfiI GAWTC 2 cut(s) 614, 752
Tru1I TTAA 4 cut(s) 440, 489, 567, 810
Tru9I TTAA 4 cut(s) 440, 489, 567, 810
TscAI CASTG 1 cut(s) 694
TseI GCWGC 5 cut(s) 11, 155, 169, 584, 974
TspDTI ATGAA 5 cut(s) 68, 89, 568, 887, 935
TspRI CASTG 1 cut(s) 694
Vha464I CTTAAG 1 cut(s) 488
VpaK11BI GGWCC 1 cut(s) 377
XagI CCTNNNNNAGG 2 cut(s) 387, 488
XapI RAATTY 1 cut(s) 326
XbaI TCTAGA 1 cut(s) 931
XceI RCATGY 1 cut(s) 85
XcmI CCANNNNNNNNNTGG 1 cut(s) 470
XmiI GTMKAC 1 cut(s) 510
XspI CTAG 2 cut(s) 459, 932
Zsp2I ATGCAT 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.