pycom05g25420

Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002020
Physical Location & Seq
Reverse (-)
26777319 .. 26780893
3575 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 675 bp
ATGAAGAGGAGCAATGAGAAGGCATTAAAAATTGAGCATGAGTTGTGCCAAAAGATCAAGGCATGGCTTGAGGATGGGAAAGATGTCCGTCTAGGGGAGTGGAAAGCTGCTGAAGTTCCATGGGAGATCCTGAATACCTTTCAATTGCTAACAGCCAAGCCTGTTGTTTACTTGGTTAACATGAATGAGAAAGATTACCAGAGGAAAAAGAACAAGTTCTTGCCGAAGATCCATGCATGGGTGCAGGAACATGGAGCTGAACCAATTATTCCATTCAGCTGTGCATTAGAAAGGAATCTTGCTGATTTGCCACCTGAAGAAGCAGCAAAGTATTGTGAGCAGAACAACGTGCAAAGTGCCCTTACCAAGATCATAAAAACTGGATTTTCAGCGATTAATCTGATATACTTTTTCACTGCTGGACCTGATGAGGTGAAGTGCTGGCAAATTAGGCGGCAGACGAAAGCTCCTCAAGCTGCAGGGGCCATCCATACTGATTTCGAGAGAGGCTTCATTTGTGCTGAGGTCATGAAATTTGATGATCTGAAGGAACTCGGAAGTGAGCCAGCTGTTAAGGCTGCTGGTAAGTACAGACAGGAGGGGAAGACATACGTGGTCCAAGATGCAGACGTCATATTCTTCAAGTTTAATGTTTCCGGGGGCGGGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1 YchF-GTPase_C

Protein Analysis

225

Amino Acids

25.48

Weight (kDa)

8.67

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YchF-GTPase_C PF06071 134 - 217 2.6e-38 Protein of unknown function (DUF933)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 633
AciI CCGC 2 cut(s) 454, 663
AclWI GGATC 2 cut(s) 121, 223
AcsI RAATTY 1 cut(s) 533
AcuI CTGAAG 3 cut(s) 132, 336, 566
AcyI GRCGYC 1 cut(s) 630
AfaI GTAC 1 cut(s) 590
AfiI CCNNNNNNNGG 3 cut(s) 94, 238, 663
AgsI TTSAA 2 cut(s) 143, 643
AluBI AGCT 6 cut(s) 107, 257, 279, 467, 476, 569
AluI AGCT 6 cut(s) 107, 257, 279, 467, 476, 569
AlwI GGATC 2 cut(s) 121, 223
AoxI GGCC 1 cut(s) 483
ApeKI GCWGC 4 cut(s) 107, 323, 476, 578
ApoI RAATTY 1 cut(s) 533
AseI ATTAAT 1 cut(s) 396
Asp700I GAANNNNTTC 1 cut(s) 215
AspS9I GGNCC 3 cut(s) 422, 483, 616
AsuC2I CCSGG 1 cut(s) 658
AsuHPI GGTGA 1 cut(s) 445
AvaII GGWCC 2 cut(s) 422, 616
BaeGI GKGCMC 1 cut(s) 361
BbsI GAAGAC 1 cut(s) 611
BbvCI CCTCAGC 1 cut(s) 522
BbvI GCAGC 4 cut(s) 94, 335, 463, 565
BccI CCATC 2 cut(s) 68, 494
BcnI CCSGG 1 cut(s) 658
BfaI CTAG 1 cut(s) 92
BfmI CTRYAG 1 cut(s) 477
BisI GCNGC 5 cut(s) 108, 324, 455, 477, 579
BlsI GCNGC 5 cut(s) 109, 325, 456, 478, 580
Bme1390I CCNGG 1 cut(s) 658
Bme18I GGWCC 2 cut(s) 422, 616
BmgT120I GGNCC 3 cut(s) 422, 483, 616
BmiI GGNNCC 1 cut(s) 484
BmrFI CCNGG 1 cut(s) 658
BmsI GCATC 1 cut(s) 613
BpiI GAAGAC 1 cut(s) 611
Bpu10I CCTNAGC 1 cut(s) 522
BpuEI CTTGAG 2 cut(s) 89, 456
BpuMI CCSGG 1 cut(s) 658
BsaAI YACGTR 1 cut(s) 613
BsaHI GRCGYC 1 cut(s) 630
BsaJI CCNNGG 2 cut(s) 119, 657
BsaXI ACNNNNNCTCC 2 cut(s) 451, 481
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 238, 663
Bse1I ACTGG 1 cut(s) 385
Bse3DI GCAATG 1 cut(s) 19
BseDI CCNNGG 2 cut(s) 119, 657
BseGI GGATG 2 cut(s) 79, 486
BseLI CCNNNNNNNGG 3 cut(s) 94, 238, 663
BseMI GCAATG 1 cut(s) 19
BseMII CTCAG 1 cut(s) 513
BseNI ACTGG 1 cut(s) 385
BseRI GAGGAG 2 cut(s) 22, 459
BseSI GKGCMC 1 cut(s) 361
BseXI GCAGC 4 cut(s) 94, 335, 463, 565
BsgI GTGCAG 1 cut(s) 263
BshFI GGCC 1 cut(s) 485
BsiSI CCGG 1 cut(s) 657
BslI CCNNNNNNNGG 3 cut(s) 94, 238, 663
BsnI GGCC 1 cut(s) 485
Bsp1286I GDGCHC 1 cut(s) 361
Bsp143I GATC 5 cut(s) 54, 126, 228, 369, 541
Bsp19I CCATGG 1 cut(s) 119
BspACI CCGC 2 cut(s) 454, 663
BspANI GGCC 1 cut(s) 485
BspCNI CTCAG 1 cut(s) 514
BspHI TCATGA 1 cut(s) 528
BspLI GGNNCC 1 cut(s) 484
BspMAI CTGCAG 1 cut(s) 481
BspPI GGATC 2 cut(s) 121, 223
BsrDI GCAATG 1 cut(s) 19
BsrI ACTGG 1 cut(s) 385
BssECI CCNNGG 2 cut(s) 119, 657
BssMI GATC 5 cut(s) 54, 126, 228, 369, 541
BssNI GRCGYC 1 cut(s) 630
BssT1I CCWWGG 1 cut(s) 119
BstACI GRCGYC 1 cut(s) 630
BstBAI YACGTR 1 cut(s) 613
BstC8I GCNNGC 2 cut(s) 443, 567
BstDEI CTNAG 1 cut(s) 522
BstDSI CCRYGG 1 cut(s) 119
BstF5I GGATG 2 cut(s) 79, 486
BstKTI GATC 5 cut(s) 57, 129, 231, 372, 544
BstMBI GATC 5 cut(s) 54, 126, 228, 369, 541
BstMWI GCNNNNNNNGC 4 cut(s) 451, 473, 482, 575
BstSCI CCNGG 1 cut(s) 656
BstSFI CTRYAG 1 cut(s) 477
BstSLI GKGCMC 1 cut(s) 361
BstV1I GCAGC 4 cut(s) 94, 335, 463, 565
BstV2I GAAGAC 1 cut(s) 611
BstX2I RGATCY 2 cut(s) 126, 228
BstYI RGATCY 2 cut(s) 126, 228
BsuRI GGCC 1 cut(s) 485
BtgI CCRYGG 1 cut(s) 119
BtsCI GGATG 2 cut(s) 79, 486
BtsI GCAGTG 1 cut(s) 414
BtsIMutI CAGTG 1 cut(s) 414
Cac8I GCNNGC 2 cut(s) 443, 567
CciI TCATGA 1 cut(s) 528
Cfr13I GGNCC 3 cut(s) 422, 483, 616
Csp6I GTAC 1 cut(s) 589
CviAII CATG 8 cut(s) 38, 63, 120, 181, 233, 237, 251, 529
CviQI GTAC 1 cut(s) 589
DdeI CTNAG 1 cut(s) 522
DpnI GATC 5 cut(s) 56, 128, 230, 371, 543
DpnII GATC 5 cut(s) 54, 126, 228, 369, 541
Eco130I CCWWGG 1 cut(s) 119
Eco47I GGWCC 2 cut(s) 422, 616
Eco57I CTGAAG 3 cut(s) 132, 336, 566
EcoT14I CCWWGG 1 cut(s) 119
EcoT22I ATGCAT 1 cut(s) 238
ErhI CCWWGG 1 cut(s) 119
FaeI CATG 8 cut(s) 41, 66, 123, 184, 236, 240, 254, 532
FatI CATG 8 cut(s) 37, 62, 119, 180, 232, 236, 250, 528
FauI CCCGC 1 cut(s) 656
Fnu4HI GCNGC 5 cut(s) 108, 324, 455, 477, 579
FokI GGATG 2 cut(s) 86, 473
Fsp4HI GCNGC 5 cut(s) 108, 324, 455, 477, 579
FspBI CTAG 1 cut(s) 92
GluI GCNGC 5 cut(s) 108, 324, 455, 477, 579
HaeIII GGCC 1 cut(s) 485
HapII CCGG 1 cut(s) 657
Hin1I GRCGYC 1 cut(s) 630
Hin1II CATG 8 cut(s) 41, 66, 123, 184, 236, 240, 254, 532
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HinfI GANTC 1 cut(s) 295
HpaI GTTAAC 1 cut(s) 178
HpaII CCGG 1 cut(s) 657
HphI GGTGA 1 cut(s) 445
Hpy166II GTNNAC 2 cut(s) 169, 178
Hpy188I TCNGA 3 cut(s) 402, 546, 557
Hpy188III TCNNGA 3 cut(s) 130, 502, 529
Hpy8I GTNNAC 2 cut(s) 169, 178
HpyAV CCTTC 2 cut(s) 13, 541
HpyCH4IV ACGT 3 cut(s) 348, 612, 630
HpyCH4V TGCA 6 cut(s) 236, 244, 284, 352, 479, 626
HpyF10VI GCNNNNNNNGC 4 cut(s) 451, 473, 482, 575
HpyF3I CTNAG 1 cut(s) 522
HpySE526I ACGT 3 cut(s) 348, 612, 630
Hsp92I GRCGYC 1 cut(s) 630
Hsp92II CATG 8 cut(s) 41, 66, 123, 184, 236, 240, 254, 532
KspAI GTTAAC 1 cut(s) 178
Kzo9I GATC 5 cut(s) 54, 126, 228, 369, 541
LmnI GCTCC 3 cut(s) 9, 254, 472
Lsp1109I GCAGC 4 cut(s) 94, 335, 463, 565
LweI GCATC 1 cut(s) 613
MaeI CTAG 1 cut(s) 92
MaeII ACGT 3 cut(s) 348, 612, 630
MalI GATC 5 cut(s) 56, 128, 230, 371, 543
MboI GATC 5 cut(s) 54, 126, 228, 369, 541
MboII GAAGA 5 cut(s) 16, 238, 329, 616, 631
MfeI CAATTG 1 cut(s) 143
MflI RGATCY 2 cut(s) 126, 228
MhlI GDGCHC 1 cut(s) 361
MluCI AATT 5 cut(s) 30, 143, 264, 447, 533
MnlI CCTC 7 cut(s) 64, 195, 424, 480, 500, 517, 592
Mph1103I ATGCAT 1 cut(s) 238
MroXI GAANNNNTTC 1 cut(s) 215
MseI TTAA 5 cut(s) 26, 177, 396, 573, 648
MspA1I CMGCKG 2 cut(s) 279, 569
MspI CCGG 1 cut(s) 657
MspR9I CCNGG 1 cut(s) 658
MunI CAATTG 1 cut(s) 143
MwoI GCNNNNNNNGC 4 cut(s) 451, 473, 482, 575
NciI CCSGG 1 cut(s) 658
NcoI CCATGG 1 cut(s) 119
NdeII GATC 5 cut(s) 54, 126, 228, 369, 541
NlaIII CATG 8 cut(s) 41, 66, 123, 184, 236, 240, 254, 532
NlaIV GGNNCC 1 cut(s) 484
NsiI ATGCAT 1 cut(s) 238
PagI TCATGA 1 cut(s) 528
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 1 cut(s) 295
PkrI GCNGC 5 cut(s) 109, 325, 456, 478, 580
Ppu21I YACGTR 1 cut(s) 613
PshBI ATTAAT 1 cut(s) 396
PspN4I GGNNCC 1 cut(s) 484
PspPI GGNCC 3 cut(s) 422, 483, 616
PstI CTGCAG 1 cut(s) 481
PsuI RGATCY 2 cut(s) 126, 228
PvuII CAGCTG 2 cut(s) 279, 569
RsaI GTAC 1 cut(s) 590
RsaNI GTAC 1 cut(s) 589
SaqAI TTAA 5 cut(s) 26, 177, 396, 573, 648
SatI GCNGC 5 cut(s) 108, 324, 455, 477, 579
Sau3AI GATC 5 cut(s) 54, 126, 228, 369, 541
Sau96I GGNCC 3 cut(s) 422, 483, 616
ScrFI CCNGG 1 cut(s) 658
SduI GDGCHC 1 cut(s) 361
SfaNI GCATC 1 cut(s) 613
SfcI CTRYAG 1 cut(s) 477
SinI GGWCC 2 cut(s) 422, 616
SmlI CTYRAG 2 cut(s) 68, 471
SmoI CTYRAG 2 cut(s) 68, 471
Sse9I AATT 5 cut(s) 30, 143, 264, 447, 533
SsiI CCGC 2 cut(s) 454, 663
SspMI CTAG 1 cut(s) 92
StyD4I CCNGG 1 cut(s) 656
StyI CCWWGG 1 cut(s) 119
TaiI ACGT 3 cut(s) 351, 615, 633
TaqI TCGA 1 cut(s) 501
TasI AATT 5 cut(s) 30, 143, 264, 447, 533
TatI WGTACW 1 cut(s) 588
TauI GCSGC 1 cut(s) 457
TfiI GAWTC 1 cut(s) 295
Tru1I TTAA 5 cut(s) 26, 177, 396, 573, 648
Tru9I TTAA 5 cut(s) 26, 177, 396, 573, 648
TscAI CASTG 1 cut(s) 421
TseI GCWGC 4 cut(s) 107, 323, 476, 578
TspDTI ATGAA 4 cut(s) 17, 197, 502, 545
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 1 cut(s) 421
VpaK11BI GGWCC 2 cut(s) 422, 616
VspI ATTAAT 1 cut(s) 396
XapI RAATTY 1 cut(s) 533
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 1 cut(s) 92
ZraI GACGTC 1 cut(s) 631
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.