pycom06g00380

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001551
Physical Location & Seq
Forward (+)
363961 .. 366030
2070 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1026 bp
ATGGAGATGATGGCGGCACCGGCGGAGGAGGAAAAGGAAAGAGTATGTGTAACTGGGGCTGGAGGATTTGTGGGCTCTTGGATTGTCAAGCTTCTTCTCTCTAGCGATTATCTTGTCCACGGAACCGTCCGAAAACCCCCAGGGGATAGCAAGTATGCTCACTTGAGCAAGCTTGAGAAAGCATCAGAGAATCTGAAACTGTTCAAGGCAGATTTGCTGGACTACAATTCACTATGTTCGGCAGTTGCAGGATGCAGCGGAGTTTTCGATGTTGCCAGCCCTGTAATTCCTAGTGCTAGTGTAACAAATCCTCAGGCAAGAGTACCTAATTCACTGATACAAGATGCTTGCCTAGAAGCCAAAGTGAAACGAGTTGTTGTTGTGTCCTCTATATCCGCTCTTTTTGTGAGCCCGGACTGGCCTAAGGGTCAAGTCAAGGACGAGACTTGTTGGTCTGTTCCCGAATATATGAAAGCAACCAAGAAATGGTATTTTCTTTCAAAAACGTTAGCAGAACGGGAGGCTTTGGAGTTTGGGAAAAGAACTGGACTTGAAATCATAACTGTTTTACCTTCTCTTAATTTGAGGCCGATTTTGCAACCCACCATGAATGCAAGTTGCTGGTTTCTAGCAAAGGTTCTTGAAGGTTACGAGGGTATATATGTTTCTTATTTGTGCAATGTGGCTATGTACCTAGGCGGGTTTGAGTCAATTGGATACAATTATTGGACGGTGGTGGATGTTCGTGATGTAGCTGAAGCAGTGCTTCTTGTATACAAGAAATCTGAGGCTGGTGAACGATACATATGCACAGCATATGCTATTGGAATTAAGGATTTGGTGGACAACTACTTGAGGGTTGCATATCCTAACTACAATTATCCCCACGACCTTAGCCACACAGAGGAAGAAGAAAAAAACGTGAGCTCGGGTAAATTGCAGAGGTTGGGTTGGAGTTACCGGCCACTCAAGGAGACTCTTATTGATTCCATTGAAAGATACCGGAAGGCTAGCGTCTTGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

342

Amino Acids

38.05

Weight (kDa)

6.18

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 12 - 129 5.4e-08 NmrA-like family
Epimerase PF01370 15 - 268 2.6e-16 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 17 - 281 2.6e-11 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 17 - 177 7.5e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 19 - 138 1.4e-11 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 451
AccB1I GGYRCC 1 cut(s) 16
AccB7I CCANNNNNTGG 1 cut(s) 486
AccBSI CCGCTC 1 cut(s) 398
AccI GTMKAC 1 cut(s) 774
AciI CCGC 5 cut(s) 14, 23, 258, 396, 699
AclI AACGTT 1 cut(s) 506
AcoI YGGCCR 1 cut(s) 962
AcuI CTGAAG 1 cut(s) 777
AfaI GTAC 2 cut(s) 324, 692
AfiI CCNNNNNNNGG 2 cut(s) 486, 904
AgsI TTSAA 5 cut(s) 205, 501, 554, 644, 995
AjnI CCWGG 1 cut(s) 139
AluBI AGCT 4 cut(s) 91, 172, 755, 927
AluI AGCT 4 cut(s) 91, 172, 755, 927
Alw21I GWGCWC 1 cut(s) 929
Alw26I GTCTC 2 cut(s) 437, 968
Ama87I CYCGRG 1 cut(s) 928
AoxI GGCC 3 cut(s) 419, 587, 962
ApeKI GCWGC 1 cut(s) 255
Asp700I GAANNNNTTC 1 cut(s) 200
AspA2I CCTAGG 1 cut(s) 694
AsuC2I CCSGG 1 cut(s) 413
AsuHPI GGTGA 1 cut(s) 806
AsuNHI GCTAGC 1 cut(s) 1010
AvaI CYCGRG 1 cut(s) 928
AvrII CCTAGG 1 cut(s) 694
AxyI CCTNAGG 2 cut(s) 312, 423
BanI GGYRCC 1 cut(s) 16
BanII GRGCYC 3 cut(s) 77, 413, 929
Bbv12I GWGCWC 1 cut(s) 929
BbvI GCAGC 1 cut(s) 267
BccI CCATC 1 cut(s) 4
BcgI CGANNNNNNTGC 2 cut(s) 789, 823
BciT130I CCWGG 1 cut(s) 141
BciVI GTATCC 1 cut(s) 710
BcnI CCSGG 1 cut(s) 413
BcoDI GTCTC 2 cut(s) 437, 968
BfaI CTAG 7 cut(s) 102, 291, 297, 353, 629, 695, 1011
BfuI GTATCC 1 cut(s) 710
BisI GCNGC 2 cut(s) 15, 256
BlnI CCTAGG 1 cut(s) 694
BlsI GCNGC 2 cut(s) 16, 257
Bme1390I CCNGG 2 cut(s) 141, 413
BmeT110I CYCGRG 1 cut(s) 928
BmiI GGNNCC 2 cut(s) 18, 124
BmrFI CCNGG 2 cut(s) 141, 413
BmrI ACTGGG 1 cut(s) 63
BmsI GCATC 3 cut(s) 191, 242, 334
BmtI GCTAGC 1 cut(s) 1014
BmuI ACTGGG 1 cut(s) 63
BpmI CTGGAG 1 cut(s) 81
Bpu10I CCTNAGC 1 cut(s) 893
BpuEI CTTGAG 4 cut(s) 184, 194, 874, 953
BpuMI CCSGG 1 cut(s) 413
BsaJI CCNNGG 4 cut(s) 118, 139, 140, 694
BsaWI WCCGGW 1 cut(s) 1002
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bsc4I CCNNNNNNNGG 2 cut(s) 486, 904
Bse118I RCCGGY 2 cut(s) 19, 960
Bse1I ACTGG 3 cut(s) 58, 422, 550
Bse21I CCTNAGG 2 cut(s) 312, 423
Bse3DI GCAATG 1 cut(s) 685
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 4 cut(s) 118, 139, 140, 694
BseGI GGATG 2 cut(s) 257, 745
BseLI CCNNNNNNNGG 2 cut(s) 486, 904
BseMI GCAATG 1 cut(s) 685
BseMII CTCAG 2 cut(s) 326, 777
BseNI ACTGG 3 cut(s) 58, 422, 550
BseRI GAGGAG 1 cut(s) 41
BseXI GCAGC 1 cut(s) 267
BshFI GGCC 3 cut(s) 421, 589, 964
BshNI GGYRCC 1 cut(s) 16
BsiHKAI GWGCWC 1 cut(s) 929
BsiHKCI CYCGRG 1 cut(s) 928
BsiSI CCGG 4 cut(s) 20, 413, 961, 1003
BslI CCNNNNNNNGG 2 cut(s) 486, 904
BsmAI GTCTC 2 cut(s) 437, 968
BsmI GAATGC 1 cut(s) 616
BsnI GGCC 3 cut(s) 421, 589, 964
BsoBI CYCGRG 1 cut(s) 928
Bsp1286I GDGCHC 3 cut(s) 77, 413, 929
BspACI CCGC 5 cut(s) 14, 23, 258, 396, 699
BspANI GGCC 3 cut(s) 421, 589, 964
BspCNI CTCAG 2 cut(s) 325, 778
BspLI GGNNCC 2 cut(s) 18, 124
BspOI GCTAGC 1 cut(s) 1014
BspT107I GGYRCC 1 cut(s) 16
BsrBI CCGCTC 1 cut(s) 398
BsrDI GCAATG 1 cut(s) 685
BsrFI RCCGGY 2 cut(s) 19, 960
BsrI ACTGG 3 cut(s) 58, 422, 550
BssAI RCCGGY 2 cut(s) 19, 960
BssECI CCNNGG 4 cut(s) 118, 139, 140, 694
BssNAI GTATAC 1 cut(s) 775
BssT1I CCWWGG 1 cut(s) 694
Bst1107I GTATAC 1 cut(s) 775
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 4 cut(s) 127, 201, 565, 733
BstC8I GCNNGC 4 cut(s) 170, 277, 349, 1012
BstDEI CTNAG 4 cut(s) 312, 423, 786, 893
BstDSI CCRYGG 1 cut(s) 118
BstF5I GGATG 2 cut(s) 257, 745
BstMAI GTCTC 2 cut(s) 437, 968
BstMWI GCNNNNNNNGC 2 cut(s) 20, 595
BstNI CCWGG 1 cut(s) 141
BstSCI CCNGG 2 cut(s) 139, 411
BstV1I GCAGC 1 cut(s) 267
BstZ17I GTATAC 1 cut(s) 775
Bsu36I CCTNAGG 2 cut(s) 312, 423
BsuI GTATCC 1 cut(s) 710
BsuRI GGCC 3 cut(s) 421, 589, 964
BtgI CCRYGG 1 cut(s) 118
BtsCI GGATG 2 cut(s) 257, 745
BtsI GCAGTG 1 cut(s) 768
BtsIMutI CAGTG 2 cut(s) 332, 768
Cac8I GCNNGC 4 cut(s) 170, 277, 349, 1012
Cfr10I RCCGGY 2 cut(s) 19, 960
CseI GACGC 1 cut(s) 1003
Csp6I GTAC 2 cut(s) 323, 691
CviAII CATG 1 cut(s) 607
CviQI GTAC 2 cut(s) 323, 691
DdeI CTNAG 4 cut(s) 312, 423, 786, 893
DrdI GACNNNNNNGTC 1 cut(s) 451
DseDI GACNNNNNNGTC 1 cut(s) 451
EaeI YGGCCR 1 cut(s) 962
EciI GGCGGA 1 cut(s) 38
Ecl136II GAGCTC 1 cut(s) 927
Eco130I CCWWGG 1 cut(s) 694
Eco24I GRGCYC 3 cut(s) 77, 413, 929
Eco53kI GAGCTC 1 cut(s) 927
Eco57I CTGAAG 1 cut(s) 777
Eco81I CCTNAGG 2 cut(s) 312, 423
Eco88I CYCGRG 1 cut(s) 928
EcoICRI GAGCTC 1 cut(s) 927
EcoRII CCWGG 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 694
EcoT38I GRGCYC 3 cut(s) 77, 413, 929
ErhI CCWWGG 1 cut(s) 694
FaeI CATG 1 cut(s) 610
FalI AAGNNNNNCTT 2 cut(s) 750, 782
FatI CATG 1 cut(s) 606
FauI CCCGC 1 cut(s) 692
FauNDI CATATG 2 cut(s) 806, 817
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 2 cut(s) 15, 256
FokI GGATG 2 cut(s) 264, 752
FriOI GRGCYC 3 cut(s) 77, 413, 929
Fsp4HI GCNGC 2 cut(s) 15, 256
FspBI CTAG 7 cut(s) 102, 291, 297, 353, 629, 695, 1011
GluI GCNGC 2 cut(s) 15, 256
GsuI CTGGAG 1 cut(s) 81
HaeIII GGCC 3 cut(s) 421, 589, 964
HapII CCGG 4 cut(s) 20, 413, 961, 1003
HgaI GACGC 1 cut(s) 1003
Hin1II CATG 1 cut(s) 610
HindIII AAGCTT 2 cut(s) 89, 170
HinfI GANTC 4 cut(s) 190, 707, 976, 986
HpaII CCGG 4 cut(s) 20, 413, 961, 1003
HphI GGTGA 1 cut(s) 806
Hpy166II GTNNAC 4 cut(s) 118, 775, 797, 844
Hpy188I TCNGA 4 cut(s) 131, 187, 195, 787
Hpy188III TCNNGA 3 cut(s) 461, 641, 746
Hpy8I GTNNAC 4 cut(s) 118, 775, 797, 844
HpyAV CCTTC 3 cut(s) 582, 638, 1000
HpyCH4III ACNGT 4 cut(s) 127, 201, 565, 733
HpyCH4IV ACGT 2 cut(s) 506, 921
HpyCH4V TGCA 8 cut(s) 248, 255, 598, 614, 678, 810, 863, 940
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 595
HpyF3I CTNAG 4 cut(s) 312, 423, 786, 893
HpySE526I ACGT 2 cut(s) 506, 921
Hsp92II CATG 1 cut(s) 610
Lsp1109I GCAGC 1 cut(s) 267
LweI GCATC 3 cut(s) 191, 242, 334
MaeI CTAG 7 cut(s) 102, 291, 297, 353, 629, 695, 1011
MaeII ACGT 2 cut(s) 506, 921
MaeIII GTNAC 4 cut(s) 49, 301, 647, 956
MbiI CCGCTC 1 cut(s) 398
MboII GAAGA 3 cut(s) 86, 920, 923
MfeI CAATTG 1 cut(s) 711
MhlI GDGCHC 3 cut(s) 77, 413, 929
MluCI AATT 9 cut(s) 226, 285, 328, 580, 711, 721, 828, 877, 935
MlyI GAGTC 2 cut(s) 716, 970
MmeI TCCRAC 1 cut(s) 932
MroXI GAANNNNTTC 1 cut(s) 200
MseI TTAA 2 cut(s) 579, 831
MspA1I CMGCKG 1 cut(s) 258
MspI CCGG 4 cut(s) 20, 413, 961, 1003
MspR9I CCNGG 2 cut(s) 141, 413
MunI CAATTG 1 cut(s) 711
Mva1269I GAATGC 1 cut(s) 616
MvaI CCWGG 1 cut(s) 141
MwoI GCNNNNNNNGC 2 cut(s) 20, 595
NciI CCSGG 1 cut(s) 413
NdeI CATATG 2 cut(s) 806, 817
NheI GCTAGC 1 cut(s) 1010
NlaIII CATG 1 cut(s) 610
NlaIV GGNNCC 2 cut(s) 18, 124
PasI CCCWGGG 1 cut(s) 140
PctI GAATGC 1 cut(s) 616
PdmI GAANNNNTTC 1 cut(s) 200
PfeI GAWTC 2 cut(s) 190, 986
PflMI CCANNNNNTGG 1 cut(s) 486
PkrI GCNGC 2 cut(s) 16, 257
PleI GAGTC 2 cut(s) 715, 970
PpsI GAGTC 2 cut(s) 715, 970
Psp124BI GAGCTC 1 cut(s) 929
Psp1406I AACGTT 1 cut(s) 506
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspN4I GGNNCC 2 cut(s) 18, 124
RsaI GTAC 2 cut(s) 324, 692
RsaNI GTAC 2 cut(s) 323, 691
SacI GAGCTC 1 cut(s) 929
SaqAI TTAA 2 cut(s) 579, 831
SatI GCNGC 2 cut(s) 15, 256
SchI GAGTC 2 cut(s) 716, 970
ScrFI CCNGG 2 cut(s) 141, 413
SduI GDGCHC 3 cut(s) 77, 413, 929
SfaNI GCATC 3 cut(s) 191, 242, 334
SgrAI CRCCGGYG 1 cut(s) 19
SmlI CTYRAG 4 cut(s) 163, 173, 853, 968
SmoI CTYRAG 4 cut(s) 163, 173, 853, 968
Sse9I AATT 9 cut(s) 226, 285, 328, 580, 711, 721, 828, 877, 935
SsiI CCGC 5 cut(s) 14, 23, 258, 396, 699
SspMI CTAG 7 cut(s) 102, 291, 297, 353, 629, 695, 1011
SstI GAGCTC 1 cut(s) 929
StyD4I CCNGG 2 cut(s) 139, 411
StyI CCWWGG 1 cut(s) 694
TaaI ACNGT 4 cut(s) 127, 201, 565, 733
TaiI ACGT 2 cut(s) 509, 924
TaqI TCGA 1 cut(s) 267
TasI AATT 9 cut(s) 226, 285, 328, 580, 711, 721, 828, 877, 935
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 2 cut(s) 190, 986
Tru1I TTAA 2 cut(s) 579, 831
Tru9I TTAA 2 cut(s) 579, 831
TscAI CASTG 2 cut(s) 339, 768
TseI GCWGC 1 cut(s) 255
TspDTI ATGAA 2 cut(s) 485, 623
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 2 cut(s) 339, 768
Van91I CCANNNNNTGG 1 cut(s) 486
XmaJI CCTAGG 1 cut(s) 694
XmiI GTMKAC 1 cut(s) 774
XmnI GAANNNNTTC 1 cut(s) 200
XspI CTAG 7 cut(s) 102, 291, 297, 353, 629, 695, 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.