pycom06g07650

RNA-binding protein NOB1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001896
Physical Location & Seq
Reverse (-)
12501133 .. 12502354
1222 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g07650.1

Sequence Viewer

Length: 990 bp
ATGGCAGTGGTCGACGCTAATGCCATCATTCAAGGCGGAGAGAGCCTCAGCCACTGTGCCGACAAGCTGGTCTCTATCCCGGAAGTCATGGACGAGGTCCGCGACCCCGTTTCCCGCCACCGGCTTGCCTTTCTTCCCTTTGAAGTCCAAACCATGGAACCCTCCCCTGAATCCCTCAACAAAGTTATTAAGTTTGCGAGGGCCACCGGAGATTTACAAACGCTTTCGGATGTGGATATCAAGCTCACTGCTCTGACCTATACGTTGGAGGCTCAGATACATGGCACTGAACATCTTAGGGACTGCCCCCCTCCGGTGCATACGGTCAATGTTAAGAGGTTGCCAGAGAAGGATTTGCCTGGTTGGGGCAATAATGTTCCAAATTTGGCAGAGTGGGAAGCTCTGGAACATGCTGAAGAGGAGAAATCAAACACTAGTTCGAGGATTCTTCCATTGAAGGATATAAACTTGAATGTGATCGATTCTGATAGCCATTCTGTTGATGGTTCTGTGGTGGAAGTTAAAAGTGATGCTCATTCTGAAAATCAGGAGGATGTTGGTGGTACTGAAAGAAGACATAGGAGAAATTTTCCCAAGAAGAAAGAGATAAGCATTGAGGGGAAGATGGTTTTGGCTGGGATTGATGCGTCCCAAGGACAGTTGGATGATAATGCTGATGACTGGATGCCTGCTGTCACTCGAAGTACGCATAGGAGGTTTTTGAGAAGAAAAGCTAGGCGCAAGTCTAATGAGTCATTATCTGAAAAGGATGCTCAAGATGCAGAGGAGGAAGCTAGAGGCAATGATCAATTGTTGCCTGTAGAATCTGAAGAAGCATGTAATAGAAATGGAATTTTTGAGGGTGGTGAGATAACAAATATAAAGAATGGCGATGAAGGCCTGTCTTCAATTCTAAAGCAAACGAGACTGGAAGAAGATTCACTAAGGGCTCTTCAAGAAGGGAAGGAGCTTAATGGGGGTAGAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
NOB1_Zn_bind PIN_6

Protein Analysis

330

Amino Acids

36.5

Weight (kDa)

5.02

Isoelectric Point (pI)

58.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIN_6 PF17146 3 - 89 1.6e-26 PIN domain of ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 68
AccB7I CCANNNNNTGG 1 cut(s) 154
AccI GTMKAC 1 cut(s) 12
AccII CGCG 1 cut(s) 102
AciI CCGC 3 cut(s) 36, 100, 115
AcsI RAATTY 3 cut(s) 382, 586, 852
AcuI CTGAAG 2 cut(s) 435, 849
AfaI GTAC 2 cut(s) 565, 706
AfiI CCNNNNNNNGG 4 cut(s) 120, 154, 313, 365
AgsI TTSAA 6 cut(s) 32, 143, 457, 472, 909, 956
AhlI ACTAGT 1 cut(s) 434
AjnI CCWGG 1 cut(s) 358
AjuI GAANNNNNNNTTGG 2 cut(s) 614, 646
AluBI AGCT 6 cut(s) 67, 244, 401, 734, 794, 970
AluI AGCT 6 cut(s) 67, 244, 401, 734, 794, 970
Alw26I GTCTC 2 cut(s) 76, 919
AlwNI CAGNNNCTG 1 cut(s) 54
AoxI GGCC 2 cut(s) 201, 898
ApoI RAATTY 3 cut(s) 382, 586, 852
AspLEI GCGC 1 cut(s) 741
AspS9I GGNCC 2 cut(s) 97, 201
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 878
AvaII GGWCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 952
BbsI GAAGAC 2 cut(s) 580, 897
BbvCI CCTCAGC 1 cut(s) 47
BccI CCATC 3 cut(s) 32, 497, 619
BcgI CGANNNNNNTGC 1 cut(s) 36
BciT130I CCWGG 1 cut(s) 360
BclI TGATCA 1 cut(s) 805
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 2 cut(s) 76, 919
BcuI ACTAGT 1 cut(s) 434
BfaI CTAG 3 cut(s) 435, 735, 795
BfmI CTRYAG 1 cut(s) 819
Bme1390I CCNGG 2 cut(s) 80, 360
Bme18I GGWCC 1 cut(s) 97
BmgT120I GGNCC 2 cut(s) 97, 201
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 2 cut(s) 80, 360
BmsI GCATC 5 cut(s) 520, 634, 675, 760, 769
BpiI GAAGAC 2 cut(s) 580, 897
BplI GAGNNNNNCTC 2 cut(s) 30, 62
Bpu10I CCTNAGC 1 cut(s) 47
BpuEI CTTGAG 1 cut(s) 759
BpuMI CCSGG 1 cut(s) 80
Bsa29I ATCGAT 1 cut(s) 480
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 2 cut(s) 153, 652
BsaWI WCCGGW 2 cut(s) 206, 313
Bsc4I CCNNNNNNNGG 4 cut(s) 120, 154, 313, 365
Bse118I RCCGGY 1 cut(s) 120
Bse1I ACTGG 2 cut(s) 686, 933
Bse3DI GCAATG 1 cut(s) 808
BseBI CCWGG 1 cut(s) 360
BseCI ATCGAT 1 cut(s) 480
BseDI CCNNGG 2 cut(s) 153, 652
BseGI GGATG 5 cut(s) 235, 559, 670, 690, 775
BseLI CCNNNNNNNGG 4 cut(s) 120, 154, 313, 365
BseMI GCAATG 1 cut(s) 808
BseMII CTCAG 2 cut(s) 61, 287
BseNI ACTGG 2 cut(s) 686, 933
BseRI GAGGAG 2 cut(s) 434, 800
BseYI CCCAGC 1 cut(s) 635
Bsh1236I CGCG 1 cut(s) 102
BshFI GGCC 2 cut(s) 203, 900
BshVI ATCGAT 1 cut(s) 480
BsiSI CCGG 4 cut(s) 80, 121, 207, 314
BslFI GGGAC 2 cut(s) 314, 634
BslI CCNNNNNNNGG 4 cut(s) 120, 154, 313, 365
BsmAI GTCTC 2 cut(s) 76, 919
BsmFI GGGAC 2 cut(s) 314, 634
BsnI GGCC 2 cut(s) 203, 900
Bso31I GGTCTC 1 cut(s) 76
Bsp1286I GDGCHC 1 cut(s) 952
Bsp143I GATC 2 cut(s) 477, 805
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 3 cut(s) 36, 100, 115
BspANI GGCC 2 cut(s) 203, 900
BspCNI CTCAG 2 cut(s) 60, 286
BspDI ATCGAT 1 cut(s) 480
BspFNI CGCG 1 cut(s) 102
BspLI GGNNCC 1 cut(s) 159
BspQI GCTCTTC 1 cut(s) 957
BspTNI GGTCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 808
BsrFI RCCGGY 1 cut(s) 120
BsrI ACTGG 2 cut(s) 686, 933
BssAI RCCGGY 1 cut(s) 120
BssECI CCNNGG 2 cut(s) 153, 652
BssMI GATC 2 cut(s) 477, 805
BssT1I CCWWGG 2 cut(s) 153, 652
Bst2UI CCWGG 1 cut(s) 360
Bst4CI ACNGT 3 cut(s) 56, 325, 660
Bst6I CTCTTC 2 cut(s) 411, 957
BstC8I GCNNGC 2 cut(s) 126, 690
BstDEI CTNAG 4 cut(s) 47, 273, 296, 944
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 5 cut(s) 235, 559, 670, 690, 775
BstFNI CGCG 1 cut(s) 102
BstHHI GCGC 1 cut(s) 741
BstKTI GATC 2 cut(s) 480, 808
BstMAI GTCTC 2 cut(s) 76, 919
BstMBI GATC 2 cut(s) 477, 805
BstMWI GCNNNNNNNGC 3 cut(s) 42, 779, 897
BstNI CCWGG 1 cut(s) 360
BstNSI RCATGY 2 cut(s) 413, 840
BstSCI CCNGG 2 cut(s) 78, 358
BstSFI CTRYAG 1 cut(s) 819
BstUI CGCG 1 cut(s) 102
BstV2I GAAGAC 2 cut(s) 580, 897
Bsu15I ATCGAT 1 cut(s) 480
BsuRI GGCC 2 cut(s) 203, 900
BsuTUI ATCGAT 1 cut(s) 480
BtgI CCRYGG 1 cut(s) 153
BtgZI GCGATG 1 cut(s) 906
BtsCI GGATG 5 cut(s) 235, 559, 670, 690, 775
BtsI GCAGTG 2 cut(s) 12, 246
BtsIMutI CAGTG 4 cut(s) 12, 52, 246, 285
Cac8I GCNNGC 2 cut(s) 126, 690
CaiI CAGNNNCTG 1 cut(s) 54
CfoI GCGC 1 cut(s) 741
Cfr10I RCCGGY 1 cut(s) 120
Cfr13I GGNCC 2 cut(s) 97, 201
ClaI ATCGAT 1 cut(s) 480
CseI GACGC 2 cut(s) 23, 636
Csp6I GTAC 2 cut(s) 564, 705
CviAII CATG 5 cut(s) 88, 154, 281, 410, 837
CviQI GTAC 2 cut(s) 564, 705
DdeI CTNAG 4 cut(s) 47, 273, 296, 944
DpnI GATC 2 cut(s) 479, 807
DpnII GATC 2 cut(s) 477, 805
DrdI GACNNNNNNGTC 1 cut(s) 68
DseDI GACNNNNNNGTC 1 cut(s) 68
Eam1104I CTCTTC 2 cut(s) 411, 957
EarI CTCTTC 2 cut(s) 411, 957
EciI GGCGGA 1 cut(s) 51
Eco130I CCWWGG 2 cut(s) 153, 652
Eco147I AGGCCT 1 cut(s) 900
Eco24I GRGCYC 1 cut(s) 952
Eco31I GGTCTC 1 cut(s) 76
Eco32I GATATC 1 cut(s) 238
Eco47I GGWCC 1 cut(s) 97
Eco57I CTGAAG 2 cut(s) 435, 849
EcoRII CCWGG 1 cut(s) 358
EcoRV GATATC 1 cut(s) 238
EcoT14I CCWWGG 2 cut(s) 153, 652
EcoT38I GRGCYC 1 cut(s) 952
ErhI CCWWGG 2 cut(s) 153, 652
FaeI CATG 5 cut(s) 91, 157, 284, 413, 840
FaqI GGGAC 2 cut(s) 314, 634
FatI CATG 5 cut(s) 87, 153, 280, 409, 836
FauI CCCGC 1 cut(s) 122
FbaI TGATCA 1 cut(s) 805
FblI GTMKAC 1 cut(s) 12
FokI GGATG 5 cut(s) 242, 566, 677, 697, 782
FriOI GRGCYC 1 cut(s) 952
FspBI CTAG 3 cut(s) 435, 735, 795
GlaI GCGC 1 cut(s) 740
GsaI CCCAGC 1 cut(s) 639
HaeIII GGCC 2 cut(s) 203, 900
HapII CCGG 4 cut(s) 80, 121, 207, 314
HgaI GACGC 2 cut(s) 23, 636
HhaI GCGC 1 cut(s) 741
Hin1II CATG 5 cut(s) 91, 157, 284, 413, 840
Hin6I GCGC 1 cut(s) 739
HinP1I GCGC 1 cut(s) 739
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 6 cut(s) 170, 445, 482, 752, 824, 938
HpaII CCGG 4 cut(s) 80, 121, 207, 314
HphI GGTGA 1 cut(s) 878
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 7 cut(s) 229, 255, 276, 487, 541, 763, 829
Hpy188III TCNNGA 4 cut(s) 404, 548, 776, 956
Hpy8I GTNNAC 1 cut(s) 13
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 5 cut(s) 343, 451, 890, 953, 958
HpyCH4III ACNGT 3 cut(s) 56, 325, 660
HpyCH4IV ACGT 1 cut(s) 263
HpyCH4V TGCA 2 cut(s) 319, 782
HpyF10VI GCNNNNNNNGC 3 cut(s) 42, 779, 897
HpyF3I CTNAG 4 cut(s) 47, 273, 296, 944
HpySE526I ACGT 1 cut(s) 263
Hsp92II CATG 5 cut(s) 91, 157, 284, 413, 840
HspAI GCGC 1 cut(s) 739
Ksp22I TGATCA 1 cut(s) 805
Kzo9I GATC 2 cut(s) 477, 805
LguI GCTCTTC 1 cut(s) 957
LmnI GCTCC 1 cut(s) 967
LweI GCATC 5 cut(s) 520, 634, 675, 760, 769
MaeI CTAG 3 cut(s) 435, 735, 795
MaeII ACGT 1 cut(s) 263
MaeIII GTNAC 1 cut(s) 694
MalI GATC 2 cut(s) 479, 807
MboI GATC 2 cut(s) 477, 805
MfeI CAATTG 1 cut(s) 809
MhlI GDGCHC 1 cut(s) 952
MluCI AATT 5 cut(s) 382, 586, 809, 852, 909
MlyI GAGTC 1 cut(s) 761
MmeI TCCRAC 2 cut(s) 246, 642
MseI TTAA 4 cut(s) 189, 333, 522, 972
MspI CCGG 4 cut(s) 80, 121, 207, 314
MspR9I CCNGG 2 cut(s) 80, 360
MunI CAATTG 1 cut(s) 809
MvaI CCWGG 1 cut(s) 360
MvnI CGCG 1 cut(s) 102
MwoI GCNNNNNNNGC 3 cut(s) 42, 779, 897
NciI CCSGG 1 cut(s) 80
NcoI CCATGG 1 cut(s) 153
NdeII GATC 2 cut(s) 477, 805
NlaIII CATG 5 cut(s) 91, 157, 284, 413, 840
NlaIV GGNNCC 1 cut(s) 159
NmuCI GTSAC 1 cut(s) 694
NspI RCATGY 2 cut(s) 413, 840
PceI AGGCCT 1 cut(s) 900
PciSI GCTCTTC 1 cut(s) 957
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PfeI GAWTC 5 cut(s) 170, 445, 482, 824, 938
PflFI GACNNNGTC 1 cut(s) 95
PflMI CCANNNNNTGG 1 cut(s) 154
PfoI TCCNGGA 1 cut(s) 78
PleI GAGTC 1 cut(s) 760
PpsI GAGTC 1 cut(s) 760
Psp6I CCWGG 1 cut(s) 358
PspFI CCCAGC 1 cut(s) 635
PspGI CCWGG 1 cut(s) 358
PspN4I GGNNCC 1 cut(s) 159
PspPI GGNCC 2 cut(s) 97, 201
PstNI CAGNNNCTG 1 cut(s) 54
PsyI GACNNNGTC 1 cut(s) 95
RsaI GTAC 2 cut(s) 565, 706
RsaNI GTAC 2 cut(s) 564, 705
SalI GTCGAC 1 cut(s) 11
SapI GCTCTTC 1 cut(s) 957
SaqAI TTAA 4 cut(s) 189, 333, 522, 972
Sau3AI GATC 2 cut(s) 477, 805
Sau96I GGNCC 2 cut(s) 97, 201
SchI GAGTC 1 cut(s) 761
ScrFI CCNGG 2 cut(s) 80, 360
SduI GDGCHC 1 cut(s) 952
SfaNI GCATC 5 cut(s) 520, 634, 675, 760, 769
SfcI CTRYAG 1 cut(s) 819
SinI GGWCC 1 cut(s) 97
SmlI CTYRAG 1 cut(s) 774
SmoI CTYRAG 1 cut(s) 774
SpeI ACTAGT 1 cut(s) 434
Sse9I AATT 5 cut(s) 382, 586, 809, 852, 909
SseBI AGGCCT 1 cut(s) 900
SsiI CCGC 3 cut(s) 36, 100, 115
SspMI CTAG 3 cut(s) 435, 735, 795
StuI AGGCCT 1 cut(s) 900
StyD4I CCNGG 2 cut(s) 78, 358
StyI CCWWGG 2 cut(s) 153, 652
TaaI ACNGT 3 cut(s) 56, 325, 660
TaiI ACGT 1 cut(s) 266
TaqI TCGA 4 cut(s) 12, 440, 480, 700
TasI AATT 5 cut(s) 382, 586, 809, 852, 909
TfiI GAWTC 5 cut(s) 170, 445, 482, 824, 938
Tru1I TTAA 4 cut(s) 189, 333, 522, 972
Tru9I TTAA 4 cut(s) 189, 333, 522, 972
TscAI CASTG 4 cut(s) 12, 59, 253, 292
TseFI GTSAC 1 cut(s) 694
Tsp45I GTSAC 1 cut(s) 694
TspDTI ATGAA 1 cut(s) 909
TspRI CASTG 4 cut(s) 12, 59, 253, 292
Tth111I GACNNNGTC 1 cut(s) 95
Van91I CCANNNNNTGG 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 97
XapI RAATTY 3 cut(s) 382, 586, 852
XceI RCATGY 2 cut(s) 413, 840
XcmI CCANNNNNNNNNTGG 1 cut(s) 500
XmiI GTMKAC 1 cut(s) 12
XspI CTAG 3 cut(s) 435, 735, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.