RchiOBHm_Chr7g0211961

RNA-binding protein NOB1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001896
Physical Location & Seq
Reverse (-)
29146427 .. 29148338
1912 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18963

Sequence Viewer

Length: 585 bp
ATGGACGAGGTCCGCGACCCTTTTTCCAGCCACCGCCTCGCCGTCATTCCCTTCCAAGTTCAAACCATGGAACCCTCCCCTGAATCCCTCAATAATGTTGTTAAGTTTGCAAGGGCTACTGGGGATTTACAGACACTGTCAGATGTGGATGTTAAGCTCATTGCTATGACTTATACATTGGAGGCTCAGATACATGGCACTGAGCACCTCAGGGACTGTCCTCCATCGGTGCATACTGTCAATGTGAGGAGGTTGCTGGAGAATGACTTGCCCGGGTGGGGAAATAACGTGCCTAATTTGGAAGAGTGGGAAGCGTCGGAGAACGAAGCGGAGGATAAGTTGAACCCCAGTTCGAGGATTCTTCCGCTGAAAGATATAAACTTGAATGTACTGGAAGTTAGGAGTGATGTTCATTTTGAGAATAAGGAGGGTGGTGAAGGCATTCAGGGGCGACATTGGAGATATTTTCCAAAGAAGAAAGAGATAAAGATTGAAGGGAAGATGGTTTCTGATGGAATTGATGCATCCCAAGGACAAGCTGATAATAATGCCGGTGATTGGATGCCTGAGATGATTTCCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
NOB1_Zn_bind PIN_6

Protein Analysis

194

Amino Acids

21.96

Weight (kDa)

4.85

Isoelectric Point (pI)

44.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIN_6 PF17146 1 - 60 2.8e-16 PIN domain of ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 15
AciI CCGC 4 cut(s) 13, 34, 329, 365
AfaI GTAC 1 cut(s) 390
AfiI CCNNNNNNNGG 3 cut(s) 278, 354, 558
AgsI TTSAA 4 cut(s) 62, 343, 385, 494
AjnI CCWGG 1 cut(s) 578
AluBI AGCT 2 cut(s) 157, 539
AluI AGCT 2 cut(s) 157, 539
Alw21I GWGCWC 1 cut(s) 207
AlwNI CAGNNNCTG 2 cut(s) 136, 216
Ama87I CYCGRG 1 cut(s) 272
Asp700I GAANNNNTTC 1 cut(s) 441
AspS9I GGNCC 1 cut(s) 10
AsuC2I CCSGG 2 cut(s) 273, 274
AsuHPI GGTGA 2 cut(s) 446, 566
AvaI CYCGRG 1 cut(s) 272
AvaII GGWCC 1 cut(s) 10
AxyI CCTNAGG 1 cut(s) 209
Bbv12I GWGCWC 1 cut(s) 207
BccI CCATC 3 cut(s) 232, 496, 506
BceAI ACGGC 1 cut(s) 26
BciT130I CCWGG 1 cut(s) 580
BcnI CCSGG 2 cut(s) 273, 274
Bme1390I CCNGG 3 cut(s) 273, 274, 580
Bme18I GGWCC 1 cut(s) 10
BmeT110I CYCGRG 1 cut(s) 272
BmgT120I GGNCC 1 cut(s) 10
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 3 cut(s) 273, 274, 580
BmrI ACTGGG 2 cut(s) 129, 342
BmsI GCATC 3 cut(s) 511, 533, 552
BmuI ACTGGG 2 cut(s) 129, 342
BpmI CTGGAG 1 cut(s) 278
BpuMI CCSGG 2 cut(s) 273, 274
BsaJI CCNNGG 3 cut(s) 66, 272, 529
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bsc4I CCNNNNNNNGG 3 cut(s) 278, 354, 558
Bse118I RCCGGY 1 cut(s) 551
Bse1I ACTGG 3 cut(s) 124, 348, 396
Bse21I CCTNAGG 1 cut(s) 209
Bse3DI GCAATG 1 cut(s) 159
BseBI CCWGG 1 cut(s) 580
BseDI CCNNGG 3 cut(s) 66, 272, 529
BseGI GGATG 3 cut(s) 154, 524, 567
BseLI CCNNNNNNNGG 3 cut(s) 278, 354, 558
BseMI GCAATG 1 cut(s) 159
BseMII CTCAG 4 cut(s) 192, 200, 223, 558
BseNI ACTGG 3 cut(s) 124, 348, 396
BseRI GAGGAG 1 cut(s) 262
Bsh1236I CGCG 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 2 cut(s) 273, 552
BslFI GGGAC 1 cut(s) 227
BslI CCNNNNNNNGG 3 cut(s) 278, 354, 558
BsmFI GGGAC 1 cut(s) 227
BsmI GAATGC 1 cut(s) 441
BsoBI CYCGRG 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 207
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 4 cut(s) 13, 34, 329, 365
BspCNI CTCAG 4 cut(s) 193, 199, 222, 559
BspFNI CGCG 1 cut(s) 15
BspLI GGNNCC 1 cut(s) 72
BsrDI GCAATG 1 cut(s) 159
BsrFI RCCGGY 1 cut(s) 551
BsrI ACTGG 3 cut(s) 124, 348, 396
BssAI RCCGGY 1 cut(s) 551
BssECI CCNNGG 3 cut(s) 66, 272, 529
BssT1I CCWWGG 2 cut(s) 66, 529
Bst2UI CCWGG 1 cut(s) 580
Bst4CI ACNGT 3 cut(s) 138, 218, 238
Bst6I CTCTTC 1 cut(s) 297
BstDEI CTNAG 4 cut(s) 186, 201, 209, 567
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 3 cut(s) 154, 524, 567
BstFNI CGCG 1 cut(s) 15
BstNI CCWGG 1 cut(s) 580
BstSCI CCNGG 3 cut(s) 271, 272, 578
BstUI CGCG 1 cut(s) 15
Bsu36I CCTNAGG 1 cut(s) 209
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 3 cut(s) 154, 524, 567
BtsIMutI CAGTG 2 cut(s) 134, 198
CaiI CAGNNNCTG 2 cut(s) 136, 216
Cfr10I RCCGGY 1 cut(s) 551
Cfr13I GGNCC 1 cut(s) 10
Cfr9I CCCGGG 1 cut(s) 272
CseI GACGC 1 cut(s) 303
Csp6I GTAC 1 cut(s) 389
CviAII CATG 2 cut(s) 67, 194
CviJI RGCY 5 cut(s) 30, 116, 157, 185, 539
CviKI_1 RGCY 5 cut(s) 30, 116, 157, 185, 539
CviQI GTAC 1 cut(s) 389
DdeI CTNAG 4 cut(s) 186, 201, 209, 567
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Eco130I CCWWGG 2 cut(s) 66, 529
Eco47I GGWCC 1 cut(s) 10
Eco81I CCTNAGG 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 578
EcoT14I CCWWGG 2 cut(s) 66, 529
EcoT22I ATGCAT 1 cut(s) 526
ErhI CCWWGG 2 cut(s) 66, 529
FaeI CATG 2 cut(s) 70, 197
FaiI YATR 6 cut(s) 68, 167, 174, 195, 234, 377
FaqI GGGAC 1 cut(s) 227
FatI CATG 2 cut(s) 66, 193
FokI GGATG 3 cut(s) 161, 511, 574
GsuI CTGGAG 1 cut(s) 278
HapII CCGG 2 cut(s) 273, 552
HgaI GACGC 1 cut(s) 303
Hin1II CATG 2 cut(s) 70, 197
HinfI GANTC 2 cut(s) 83, 358
HpaII CCGG 2 cut(s) 273, 552
HphI GGTGA 2 cut(s) 446, 566
Hpy188I TCNGA 4 cut(s) 142, 189, 319, 511
Hpy99I CGWCG 1 cut(s) 319
HpyAV CCTTC 3 cut(s) 61, 431, 488
HpyCH4III ACNGT 3 cut(s) 138, 218, 238
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 3 cut(s) 110, 232, 524
HpyF3I CTNAG 4 cut(s) 186, 201, 209, 567
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 2 cut(s) 70, 197
LweI GCATC 3 cut(s) 511, 533, 552
MaeII ACGT 1 cut(s) 288
MboII GAAGA 4 cut(s) 314, 353, 487, 511
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 2 cut(s) 295, 516
MmeI TCCRAC 1 cut(s) 297
Mph1103I ATGCAT 1 cut(s) 526
MroXI GAANNNNTTC 1 cut(s) 441
MseI TTAA 2 cut(s) 102, 153
MslI CAYNNNNRTG 1 cut(s) 164
MspA1I CMGCKG 1 cut(s) 367
MspI CCGG 2 cut(s) 273, 552
MspR9I CCNGG 3 cut(s) 273, 274, 580
Mva1269I GAATGC 1 cut(s) 441
MvaI CCWGG 1 cut(s) 580
MvnI CGCG 1 cut(s) 15
NciI CCSGG 2 cut(s) 273, 274
NcoI CCATGG 1 cut(s) 66
NlaIII CATG 2 cut(s) 70, 197
NlaIV GGNNCC 1 cut(s) 72
NsiI ATGCAT 1 cut(s) 526
PcsI WCGNNNNNNNCGW 1 cut(s) 12
PctI GAATGC 1 cut(s) 441
PdmI GAANNNNTTC 1 cut(s) 441
PfeI GAWTC 2 cut(s) 83, 358
PflFI GACNNNGTC 2 cut(s) 8, 136
Psp6I CCWGG 1 cut(s) 578
PspGI CCWGG 1 cut(s) 578
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 10
PstNI CAGNNNCTG 2 cut(s) 136, 216
PsyI GACNNNGTC 2 cut(s) 8, 136
RsaI GTAC 1 cut(s) 390
RsaNI GTAC 1 cut(s) 389
RseI CAYNNNNRTG 1 cut(s) 164
SaqAI TTAA 2 cut(s) 102, 153
Sau96I GGNCC 1 cut(s) 10
ScrFI CCNGG 3 cut(s) 273, 274, 580
SduI GDGCHC 1 cut(s) 207
SetI ASST 6 cut(s) 12, 159, 210, 254, 291, 541
SfaNI GCATC 3 cut(s) 511, 533, 552
SinI GGWCC 1 cut(s) 10
SmaI CCCGGG 1 cut(s) 274
SmiMI CAYNNNNRTG 1 cut(s) 164
Sse9I AATT 2 cut(s) 295, 516
SsiI CCGC 4 cut(s) 13, 34, 329, 365
StyD4I CCNGG 3 cut(s) 271, 272, 578
StyI CCWWGG 2 cut(s) 66, 529
TaaI ACNGT 3 cut(s) 138, 218, 238
TaiI ACGT 1 cut(s) 291
TaqI TCGA 1 cut(s) 353
TasI AATT 2 cut(s) 295, 516
TatI WGTACW 1 cut(s) 388
TfiI GAWTC 2 cut(s) 83, 358
Tru1I TTAA 2 cut(s) 102, 153
Tru9I TTAA 2 cut(s) 102, 153
TscAI CASTG 2 cut(s) 141, 205
TspDTI ATGAA 1 cut(s) 401
TspMI CCCGGG 1 cut(s) 272
TspRI CASTG 2 cut(s) 141, 205
Tth111I GACNNNGTC 2 cut(s) 8, 136
VpaK11BI GGWCC 1 cut(s) 10
XmaI CCCGGG 1 cut(s) 272
XmnI GAANNNNTTC 1 cut(s) 441
Zsp2I ATGCAT 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.