Rh7AG020500

RNA-binding protein NOB1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001896
Physical Location & Seq
Reverse (-)
1395229 .. 1398112
2884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG020500.1

Sequence Viewer

Length: 1248 bp
ATGGACGCCACCGTCACCCCAGCCCGAGTCTCCAGTTGGAGCAACATCGTGAAGAAGCAGCCGGACCCAAAGCCCCAAAACTCCTCCTCCTCAATCGCCACCACCAAAGTCTTGGTCGAAAGCTGCAAGTCCTACAAGGGCATAGCCGTCGCCGTCGTCGACGCCAATGCTATCATTCAGGGCGGCGAGTCCCTCGCCCACCTCGCCGACAAGTTCGTCTCCATCCCCGAGGTCATCGACGACGAGGTCCGTGACCCTATTTCCCGCCACCGCCTCGCCCTCCTTCCCTTCCAAGTCCACACCATGGAGCCCTCCCCTGAATCCCGCGATAAAGTTGAAAAGTTTGCGAGGGCTACTGGGGATTTCCAGTCACTGTCAGAGGTTGATAATCGTCTTATTGCTATGACATATACACTGGAGGCTCAGATACATGGCACTGACCACCTCAGGGACTGTCCTCCGCCGGTGCATACTGTCAATGTCAGGAGGTTGCCGGAGAAGGACTTGCCCGGCTGGGAAAATAATGTCCCCAATTTGGAAGAGTGGGAAGCGCTGGAAAACAAAGCGGAGGATAAGAGCAACTCTGTTACGAGGATTCTTCCGTTGAAAGATATTAACTTGAATGTTTCTGTTGATGGTTCTGCGGTGGAAATCAGAAGTGATGTTCATTCTGAGAATCAGGAGGGTGGTGAGGGCATTCAGGGGAGATATAGGAGATATTTTCCAAAGAAGAAAGAGATAAATATTGAAGGGAAGATGGTTTCTGATGGAATTGATGCGTCCCAAGGACAAGTTGATGATAATACTGGTGACTGGATGCCTGCTGTTAGTCGGAGTACACATAGAAGGTTTTTGAGAAGAAAAGCTAGACGTGAGTCTTATGAGGCGGCATCAGCTGATAATGATCCTCAGCTACATGATGAGGAAAACACAAGTGGTGGCGTACTTGTTGATGTGAATTTTTCTGTTGAAGGAGATGAGGTCGAAATGATAAATGAAAGGCTGGATCACTTGGAGATCTCAAGCGATAATGGTGCAAGTGTTGATACATTAAATGTGAACGATGATAGTAGTGAGCAGAGCTCGATGCTGAGATCCTTGTATGAGTCTAGTGTAGCTTGTATAACAAGTGATTTTGCAATGCAAAATGTTATTCTGCAGATGGGTTTACGGTTGTTGGCACCTGGAGGAATGCAGATCCGCCAGCTGAAGAGGTATTTGTTTTATACATATACCCCATTTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
NOB1_Zn_bind PIN_6

Protein Analysis

415

Amino Acids

46.32

Weight (kDa)

4.98

Isoelectric Point (pI)

51.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIN_6 PF17146 52 - 139 5.1e-23 PIN domain of ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 11, 215, 245
AccB1I GGYRCC 1 cut(s) 1180
AccB7I CCANNNNNTGG 1 cut(s) 304
AccI GTMKAC 1 cut(s) 159
AccII CGCG 1 cut(s) 327
AciI CCGC 9 cut(s) 183, 265, 271, 325, 461, 566, 644, 887, 1201
AclWI GGATC 4 cut(s) 899, 1014, 1089, 1192
AcsI RAATTY 1 cut(s) 958
AcuI CTGAAG 1 cut(s) 1229
AcyI GRCGYC 2 cut(s) 6, 162
AfaI GTAC 2 cut(s) 838, 945
AfeI AGCGCT 1 cut(s) 552
AfiI CCNNNNNNNGG 3 cut(s) 304, 448, 535
AgsI TTSAA 5 cut(s) 338, 607, 622, 749, 971
AjiI CACGTC 1 cut(s) 872
AjnI CCWGG 1 cut(s) 1183
AluBI AGCT 7 cut(s) 123, 866, 896, 913, 1083, 1118, 1207
AluI AGCT 7 cut(s) 123, 866, 896, 913, 1083, 1118, 1207
Alw21I GWGCWC 1 cut(s) 1085
Alw26I GTCTC 2 cut(s) 34, 223
AlwI GGATC 4 cut(s) 899, 1014, 1089, 1192
AlwNI CAGNNNCTG 2 cut(s) 373, 453
Ama87I CYCGRG 2 cut(s) 24, 227
Aor51HI AGCGCT 1 cut(s) 552
ApeKI GCWGC 2 cut(s) 58, 123
ApoI RAATTY 1 cut(s) 958
ArsI GACNNNNNNTTYG 2 cut(s) 99, 131
AspLEI GCGC 1 cut(s) 553
AspS9I GGNCC 2 cut(s) 64, 247
AsuC2I CCSGG 1 cut(s) 510
AsuHPI GGTGA 3 cut(s) 7, 701, 821
AvaI CYCGRG 2 cut(s) 24, 227
AvaII GGWCC 2 cut(s) 64, 247
AxyI CCTNAGG 1 cut(s) 446
BanI GGYRCC 1 cut(s) 1180
BanII GRGCYC 2 cut(s) 312, 1085
Bbv12I GWGCWC 1 cut(s) 1085
BbvCI CCTCAGC 1 cut(s) 909
BbvI GCAGC 2 cut(s) 70, 110
BccI CCATC 5 cut(s) 230, 629, 751, 761, 1156
BceAI ACGGC 2 cut(s) 131, 137
BcgI CGANNNNNNTGC 6 cut(s) 130, 149, 164, 183, 1016, 1050
BciT130I CCWGG 1 cut(s) 1185
BcnI CCSGG 1 cut(s) 510
BcoDI GTCTC 2 cut(s) 34, 223
BfaI CTAG 2 cut(s) 867, 1110
BfmI CTRYAG 1 cut(s) 1157
BfoI RGCGCY 1 cut(s) 554
BglII AGATCT 1 cut(s) 1017
BisI GCNGC 4 cut(s) 59, 124, 184, 888
BlsI GCNGC 4 cut(s) 60, 125, 185, 889
Bme1390I CCNGG 2 cut(s) 510, 1185
Bme18I GGWCC 2 cut(s) 64, 247
BmeT110I CYCGRG 2 cut(s) 24, 227
BmgBI CACGTC 1 cut(s) 872
BmgT120I GGNCC 2 cut(s) 64, 247
BmiI GGNNCC 3 cut(s) 66, 309, 1182
BmrFI CCNGG 2 cut(s) 510, 1185
BmrI ACTGGG 1 cut(s) 366
BmsI GCATC 4 cut(s) 766, 807, 899, 1077
BmuI ACTGGG 1 cut(s) 366
BoxI GACNNNNGTC 1 cut(s) 874
BplI GAGNNNNNCTC 2 cut(s) 1067, 1099
BpmI CTGGAG 3 cut(s) 16, 437, 1206
Bpu10I CCTNAGC 1 cut(s) 909
BpuEI CTTGAG 1 cut(s) 1006
BpuMI CCSGG 1 cut(s) 510
BsaBI GATNNNNATC 1 cut(s) 903
BsaHI GRCGYC 2 cut(s) 6, 162
BsaJI CCNNGG 3 cut(s) 228, 303, 784
BsaXI ACNNNNNCTCC 4 cut(s) 71, 101, 966, 996
Bsc4I CCNNNNNNNGG 3 cut(s) 304, 448, 535
Bse118I RCCGGY 1 cut(s) 463
Bse1I ACTGG 6 cut(s) 33, 361, 367, 420, 811, 818
Bse21I CCTNAGG 1 cut(s) 446
Bse3DI GCAATG 1 cut(s) 1146
Bse8I GATNNNNATC 1 cut(s) 903
BseBI CCWGG 1 cut(s) 1185
BseDI CCNNGG 3 cut(s) 228, 303, 784
BseGI GGATG 2 cut(s) 222, 822
BseJI GATNNNNATC 1 cut(s) 903
BseLI CCNNNNNNNGG 3 cut(s) 304, 448, 535
BseMI GCAATG 1 cut(s) 1146
BseMII CTCAG 5 cut(s) 437, 460, 663, 923, 1082
BseNI ACTGG 6 cut(s) 33, 361, 367, 420, 811, 818
BseRI GAGGAG 3 cut(s) 73, 76, 79
BseXI GCAGC 2 cut(s) 70, 110
BseYI CCCAGC 2 cut(s) 19, 513
Bsh1236I CGCG 1 cut(s) 327
BshNI GGYRCC 1 cut(s) 1180
BsiHKAI GWGCWC 1 cut(s) 1085
BsiHKCI CYCGRG 2 cut(s) 24, 227
BsiSI CCGG 4 cut(s) 62, 464, 494, 510
BslFI GGGAC 4 cut(s) 175, 464, 512, 766
BslI CCNNNNNNNGG 3 cut(s) 304, 448, 535
BsmAI GTCTC 2 cut(s) 34, 223
BsmBI CGTCTC 1 cut(s) 223
BsmFI GGGAC 4 cut(s) 175, 464, 512, 766
BsmI GAATGC 2 cut(s) 696, 1197
BsoBI CYCGRG 2 cut(s) 24, 227
Bsp1286I GDGCHC 2 cut(s) 312, 1085
Bsp143I GATC 5 cut(s) 904, 1006, 1017, 1094, 1197
Bsp19I CCATGG 1 cut(s) 303
BspACI CCGC 9 cut(s) 183, 265, 271, 325, 461, 566, 644, 887, 1201
BspCNI CTCAG 5 cut(s) 436, 459, 664, 922, 1083
BspFNI CGCG 1 cut(s) 327
BspLI GGNNCC 3 cut(s) 66, 309, 1182
BspMAI CTGCAG 1 cut(s) 1161
BspPI GGATC 4 cut(s) 899, 1014, 1089, 1192
BspT107I GGYRCC 1 cut(s) 1180
BsrDI GCAATG 1 cut(s) 1146
BsrFI RCCGGY 1 cut(s) 463
BsrI ACTGG 6 cut(s) 33, 361, 367, 420, 811, 818
BssAI RCCGGY 1 cut(s) 463
BssECI CCNNGG 3 cut(s) 228, 303, 784
BssMI GATC 5 cut(s) 904, 1006, 1017, 1094, 1197
BssNI GRCGYC 2 cut(s) 6, 162
BssT1I CCWWGG 2 cut(s) 303, 784
Bst2UI CCWGG 1 cut(s) 1185
Bst4CI ACNGT 5 cut(s) 13, 375, 455, 475, 1173
Bst6I CTCTTC 2 cut(s) 534, 1205
BstACI GRCGYC 2 cut(s) 6, 162
BstC8I GCNNGC 2 cut(s) 822, 1205
BstDEI CTNAG 5 cut(s) 423, 446, 672, 909, 1091
BstDSI CCRYGG 1 cut(s) 303
BstF5I GGATG 2 cut(s) 222, 822
BstFNI CGCG 1 cut(s) 327
BstH2I RGCGCY 1 cut(s) 554
BstHHI GCGC 1 cut(s) 553
BstKTI GATC 5 cut(s) 907, 1009, 1020, 1097, 1200
BstMAI GTCTC 2 cut(s) 34, 223
BstMBI GATC 5 cut(s) 904, 1006, 1017, 1094, 1197
BstMWI GCNNNNNNNGC 2 cut(s) 203, 893
BstNI CCWGG 1 cut(s) 1185
BstPAI GACNNNNGTC 1 cut(s) 874
BstSCI CCNGG 2 cut(s) 508, 1183
BstSFI CTRYAG 1 cut(s) 1157
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 2 cut(s) 70, 110
BstX2I RGATCY 3 cut(s) 1017, 1094, 1197
BstXI CCANNNNNNTGG 1 cut(s) 112
BstYI RGATCY 3 cut(s) 1017, 1094, 1197
Bsu36I CCTNAGG 1 cut(s) 446
BtgI CCRYGG 1 cut(s) 303
BtrI CACGTC 1 cut(s) 872
BtsCI GGATG 2 cut(s) 222, 822
BtsIMutI CAGTG 3 cut(s) 371, 413, 435
Cac8I GCNNGC 2 cut(s) 822, 1205
CaiI CAGNNNCTG 2 cut(s) 373, 453
CfoI GCGC 1 cut(s) 553
Cfr10I RCCGGY 1 cut(s) 463
Cfr13I GGNCC 2 cut(s) 64, 247
CseI GACGC 3 cut(s) 14, 170, 768
Csp6I GTAC 2 cut(s) 837, 944
CviAII CATG 3 cut(s) 304, 431, 917
CviQI GTAC 2 cut(s) 837, 944
DdeI CTNAG 5 cut(s) 423, 446, 672, 909, 1091
DpnI GATC 5 cut(s) 906, 1008, 1019, 1096, 1199
DpnII GATC 5 cut(s) 904, 1006, 1017, 1094, 1197
DrdI GACNNNNNNGTC 3 cut(s) 11, 215, 245
DseDI GACNNNNNNGTC 3 cut(s) 11, 215, 245
Eam1104I CTCTTC 2 cut(s) 534, 1205
EarI CTCTTC 2 cut(s) 534, 1205
EciI GGCGGA 2 cut(s) 450, 1190
Ecl136II GAGCTC 1 cut(s) 1083
Eco130I CCWWGG 2 cut(s) 303, 784
Eco24I GRGCYC 2 cut(s) 312, 1085
Eco47I GGWCC 2 cut(s) 64, 247
Eco47III AGCGCT 1 cut(s) 552
Eco53kI GAGCTC 1 cut(s) 1083
Eco57I CTGAAG 1 cut(s) 1229
Eco81I CCTNAGG 1 cut(s) 446
Eco88I CYCGRG 2 cut(s) 24, 227
EcoICRI GAGCTC 1 cut(s) 1083
EcoRII CCWGG 1 cut(s) 1183
EcoT14I CCWWGG 2 cut(s) 303, 784
EcoT38I GRGCYC 2 cut(s) 312, 1085
ErhI CCWWGG 2 cut(s) 303, 784
Esp3I CGTCTC 1 cut(s) 223
FaeI CATG 3 cut(s) 307, 434, 920
FaqI GGGAC 4 cut(s) 175, 464, 512, 766
FatI CATG 3 cut(s) 303, 430, 916
FauI CCCGC 2 cut(s) 272, 332
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 4 cut(s) 59, 124, 184, 888
FokI GGATG 2 cut(s) 209, 829
FriOI GRGCYC 2 cut(s) 312, 1085
Fsp4HI GCNGC 4 cut(s) 59, 124, 184, 888
FspBI CTAG 2 cut(s) 867, 1110
GlaI GCGC 1 cut(s) 552
GluI GCNGC 4 cut(s) 59, 124, 184, 888
GsaI CCCAGC 2 cut(s) 23, 517
GsuI CTGGAG 3 cut(s) 16, 437, 1206
HaeII RGCGCY 1 cut(s) 554
HapII CCGG 4 cut(s) 62, 464, 494, 510
HgaI GACGC 3 cut(s) 14, 170, 768
HhaI GCGC 1 cut(s) 553
Hin1I GRCGYC 2 cut(s) 6, 162
Hin1II CATG 3 cut(s) 307, 434, 920
Hin6I GCGC 1 cut(s) 551
HinP1I GCGC 1 cut(s) 551
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 7 cut(s) 27, 188, 320, 595, 676, 875, 1106
HpaII CCGG 4 cut(s) 62, 464, 494, 510
HphI GGTGA 3 cut(s) 7, 701, 821
Hpy166II GTNNAC 5 cut(s) 160, 298, 839, 1060, 1169
Hpy188I TCNGA 6 cut(s) 379, 426, 656, 673, 766, 834
Hpy188III TCNNGA 3 cut(s) 49, 484, 680
Hpy8I GTNNAC 5 cut(s) 160, 298, 839, 1060, 1169
Hpy99I CGWCG 6 cut(s) 152, 158, 161, 164, 242, 245
HpyAV CCTTC 6 cut(s) 293, 298, 493, 743, 840, 965
HpyCH4III ACNGT 5 cut(s) 13, 375, 455, 475, 1173
HpyCH4IV ACGT 1 cut(s) 871
HpyCH4V TGCA 7 cut(s) 126, 469, 1037, 1139, 1144, 1159, 1195
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 893
HpyF3I CTNAG 5 cut(s) 423, 446, 672, 909, 1091
HpySE526I ACGT 1 cut(s) 871
Hsp92I GRCGYC 2 cut(s) 6, 162
Hsp92II CATG 3 cut(s) 307, 434, 920
HspAI GCGC 1 cut(s) 551
Kzo9I GATC 5 cut(s) 904, 1006, 1017, 1094, 1197
LmnI GCTCC 2 cut(s) 39, 307
Lsp1109I GCAGC 2 cut(s) 70, 110
LweI GCATC 4 cut(s) 766, 807, 899, 1077
MaeI CTAG 2 cut(s) 867, 1110
MaeII ACGT 1 cut(s) 871
MaeIII GTNAC 5 cut(s) 13, 251, 369, 586, 809
MalI GATC 5 cut(s) 906, 1008, 1019, 1096, 1199
MboI GATC 5 cut(s) 904, 1006, 1017, 1094, 1197
MboII GAAGA 7 cut(s) 64, 551, 590, 742, 766, 870, 1222
MflI RGATCY 3 cut(s) 1017, 1094, 1197
MhlI GDGCHC 2 cut(s) 312, 1085
MluCI AATT 3 cut(s) 532, 771, 958
MlyI GAGTC 4 cut(s) 36, 197, 884, 1115
MmeI TCCRAC 2 cut(s) 17, 812
MseI TTAA 3 cut(s) 615, 1052, 1246
MspA1I CMGCKG 2 cut(s) 896, 1207
MspI CCGG 4 cut(s) 62, 464, 494, 510
MspR9I CCNGG 2 cut(s) 510, 1185
Mva1269I GAATGC 2 cut(s) 696, 1197
MvaI CCWGG 1 cut(s) 1185
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 2 cut(s) 203, 893
NciI CCSGG 1 cut(s) 510
NcoI CCATGG 1 cut(s) 303
NdeII GATC 5 cut(s) 904, 1006, 1017, 1094, 1197
NlaIII CATG 3 cut(s) 307, 434, 920
NlaIV GGNNCC 3 cut(s) 66, 309, 1182
NmuCI GTSAC 4 cut(s) 13, 251, 369, 809
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PctI GAATGC 2 cut(s) 696, 1197
PfeI GAWTC 3 cut(s) 320, 595, 676
PflFI GACNNNGTC 1 cut(s) 245
PflMI CCANNNNNTGG 1 cut(s) 304
PkrI GCNGC 4 cut(s) 60, 125, 185, 889
PleI GAGTC 4 cut(s) 35, 196, 883, 1114
PpsI GAGTC 4 cut(s) 35, 196, 883, 1114
PshAI GACNNNNGTC 1 cut(s) 874
Psp124BI GAGCTC 1 cut(s) 1085
Psp6I CCWGG 1 cut(s) 1183
PspFI CCCAGC 2 cut(s) 19, 513
PspGI CCWGG 1 cut(s) 1183
PspN4I GGNNCC 3 cut(s) 66, 309, 1182
PspPI GGNCC 2 cut(s) 64, 247
PstI CTGCAG 1 cut(s) 1161
PstNI CAGNNNCTG 2 cut(s) 373, 453
PsuI RGATCY 3 cut(s) 1017, 1094, 1197
PsyI GACNNNGTC 1 cut(s) 245
PvuII CAGCTG 2 cut(s) 896, 1207
RsaI GTAC 2 cut(s) 838, 945
RsaNI GTAC 2 cut(s) 837, 944
SacI GAGCTC 1 cut(s) 1085
SalI GTCGAC 1 cut(s) 158
SaqAI TTAA 3 cut(s) 615, 1052, 1246
SatI GCNGC 4 cut(s) 59, 124, 184, 888
Sau3AI GATC 5 cut(s) 904, 1006, 1017, 1094, 1197
Sau96I GGNCC 2 cut(s) 64, 247
SchI GAGTC 4 cut(s) 36, 197, 884, 1115
ScrFI CCNGG 2 cut(s) 510, 1185
SduI GDGCHC 2 cut(s) 312, 1085
SfaNI GCATC 4 cut(s) 766, 807, 899, 1077
SfcI CTRYAG 1 cut(s) 1157
SgrAI CRCCGGYG 1 cut(s) 463
SgrDI CGTCGACG 1 cut(s) 158
SinI GGWCC 2 cut(s) 64, 247
SmlI CTYRAG 1 cut(s) 1021
SmoI CTYRAG 1 cut(s) 1021
Sse9I AATT 3 cut(s) 532, 771, 958
SsiI CCGC 9 cut(s) 183, 265, 271, 325, 461, 566, 644, 887, 1201
SspI AATATT 1 cut(s) 745
SspMI CTAG 2 cut(s) 867, 1110
SstI GAGCTC 1 cut(s) 1085
StyD4I CCNGG 2 cut(s) 508, 1183
StyI CCWWGG 2 cut(s) 303, 784
TaaI ACNGT 5 cut(s) 13, 375, 455, 475, 1173
TaiI ACGT 1 cut(s) 874
TaqI TCGA 5 cut(s) 117, 159, 237, 984, 1085
TasI AATT 3 cut(s) 532, 771, 958
TatI WGTACW 1 cut(s) 836
TauI GCSGC 2 cut(s) 186, 890
TfiI GAWTC 3 cut(s) 320, 595, 676
Tru1I TTAA 3 cut(s) 615, 1052, 1246
Tru9I TTAA 3 cut(s) 615, 1052, 1246
TscAI CASTG 3 cut(s) 378, 420, 442
TseFI GTSAC 4 cut(s) 13, 251, 369, 809
TseI GCWGC 2 cut(s) 58, 123
Tsp45I GTSAC 4 cut(s) 13, 251, 369, 809
TspDTI ATGAA 2 cut(s) 656, 1011
TspGWI ACGGA 2 cut(s) 239, 591
TspRI CASTG 3 cut(s) 378, 420, 442
Tth111I GACNNNGTC 1 cut(s) 245
Van91I CCANNNNNTGG 1 cut(s) 304
VpaK11BI GGWCC 2 cut(s) 64, 247
XapI RAATTY 1 cut(s) 958
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 159
XspI CTAG 2 cut(s) 867, 1110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.