Rroxscaffold_2G00143460

RNA-binding protein NOB1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001896
Physical Location & Seq
Forward (+)
81470401 .. 81471042
642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00143460.1

Sequence Viewer

Length: 642 bp
ATGGTGAAGAAGCAGCCGGAGCCAAAGCCCCAAGACTCCGACTCCTCAACCGCCACCAGCGAAGTATCCGTCGAAAGCTGCGAGGCCTCTAAGGGTATAGCCGTCGCCATCATCGATGCCAATGCCATAATTCAAGGAGGCGCGAGCCTCGGCCACTGCGCCGATAAGTTCGTCTCCATCCCCGAGGTCATGGACGAGGGCCGTGACCCTATTTCCAGCCACGTCGTTCCGTTCCAAGTCCAGACCATGGAACACTCCCCTGAATCCCTCAATAAAGTTATTAAATTTGCGAGGGCTACTGGGAATTTACAGACACTGTCAGATGTGGATGTTAAGCTCATTGCTATGACTTATACACTGGAGGCTCAGATACATGGCACTAAGCACCTCAGGGACTGTCCTCGGCAGCTGCATACGGTCAATGTCAGGAGGTTCCCGGAGAAGGACTTGCCAGGCTGGGGAAATAATATGCTTAATTTGGAAGAGTGGAAAGCACTGGAAAATGAAGCGGAGGAGGATAAGTTGAACCCTAATTCGAGGATTCTTCTGTTGAAAGATACAAACTTGAATGTTATCGTTGATTCTGAGAGCCCTTCTGTTCATTCTGAGAATCAGGAGGGTGGTGAGGGGAGCCGTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
NOB1_Zn_bind PIN_6

Protein Analysis

213

Amino Acids

23.42

Weight (kDa)

5.09

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIN_6 PF17146 37 - 120 4.4e-17 PIN domain of ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 247
AccII CGCG 1 cut(s) 143
AciI CCGC 2 cut(s) 51, 509
AcoI YGGCCR 1 cut(s) 151
AcsI RAATTY 2 cut(s) 284, 304
AfiI CCNNNNNNNGG 3 cut(s) 247, 442, 458
AgsI TTSAA 4 cut(s) 134, 526, 553, 568
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 1 cut(s) 451
AluBI AGCT 3 cut(s) 78, 337, 409
AluI AGCT 3 cut(s) 78, 337, 409
Alw26I GTCTC 1 cut(s) 178
AlwNI CAGNNNCTG 2 cut(s) 316, 396
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 3 cut(s) 84, 151, 199
ApeKI GCWGC 4 cut(s) 13, 78, 406, 409
ApoI RAATTY 2 cut(s) 284, 304
ArsI GACNNNNNNTTYG 2 cut(s) 54, 86
AspLEI GCGC 2 cut(s) 143, 161
AspS9I GGNCC 1 cut(s) 199
AsuC2I CCSGG 1 cut(s) 437
AsuHPI GGTGA 2 cut(s) 16, 635
AvaI CYCGRG 1 cut(s) 182
AxyI CCTNAGG 1 cut(s) 389
BanII GRGCYC 1 cut(s) 593
BbvI GCAGC 4 cut(s) 25, 65, 396, 418
BccI CCATC 2 cut(s) 116, 185
BceAI ACGGC 3 cut(s) 86, 186, 618
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciT130I CCWGG 1 cut(s) 453
BciVI GTATCC 1 cut(s) 76
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 1 cut(s) 178
BfuI GTATCC 1 cut(s) 76
BisI GCNGC 4 cut(s) 14, 79, 407, 410
BlsI GCNGC 4 cut(s) 15, 80, 408, 411
Bme1390I CCNGG 2 cut(s) 437, 453
BmeT110I CYCGRG 1 cut(s) 182
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 1 cut(s) 199
BmiI GGNNCC 3 cut(s) 21, 434, 632
BmrFI CCNGG 2 cut(s) 437, 453
BmrI ACTGGG 1 cut(s) 309
BmsI GCATC 1 cut(s) 106
BmuI ACTGGG 1 cut(s) 309
BpmI CTGGAG 1 cut(s) 380
BpuMI CCSGG 1 cut(s) 437
Bsa29I ATCGAT 1 cut(s) 114
BsaJI CCNNGG 4 cut(s) 148, 183, 246, 401
BsaXI ACNNNNNCTCC 4 cut(s) 26, 56, 506, 536
Bsc4I CCNNNNNNNGG 3 cut(s) 247, 442, 458
Bse1I ACTGG 3 cut(s) 304, 363, 501
Bse21I CCTNAGG 1 cut(s) 389
Bse3DI GCAATG 1 cut(s) 339
BseBI CCWGG 1 cut(s) 453
BseCI ATCGAT 1 cut(s) 114
BseDI CCNNGG 4 cut(s) 148, 183, 246, 401
BseGI GGATG 2 cut(s) 177, 334
BseLI CCNNNNNNNGG 3 cut(s) 247, 442, 458
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 4 cut(s) 380, 403, 576, 597
BseNI ACTGG 3 cut(s) 304, 363, 501
BseRI GAGGAG 2 cut(s) 34, 527
BseXI GCAGC 4 cut(s) 25, 65, 396, 418
BseYI CCCAGC 1 cut(s) 456
Bsh1236I CGCG 1 cut(s) 143
BshFI GGCC 3 cut(s) 86, 153, 201
BshVI ATCGAT 1 cut(s) 114
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 2 cut(s) 17, 437
BslFI GGGAC 1 cut(s) 407
BslI CCNNNNNNNGG 3 cut(s) 247, 442, 458
BsmAI GTCTC 1 cut(s) 178
BsmBI CGTCTC 1 cut(s) 178
BsmFI GGGAC 1 cut(s) 407
BsnI GGCC 3 cut(s) 86, 153, 201
BsoBI CYCGRG 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 593
Bsp19I CCATGG 1 cut(s) 246
BspACI CCGC 2 cut(s) 51, 509
BspANI GGCC 3 cut(s) 86, 153, 201
BspCNI CTCAG 4 cut(s) 379, 402, 577, 598
BspDI ATCGAT 1 cut(s) 114
BspFNI CGCG 1 cut(s) 143
BspLI GGNNCC 3 cut(s) 21, 434, 632
BsrDI GCAATG 1 cut(s) 339
BsrI ACTGG 3 cut(s) 304, 363, 501
BssECI CCNNGG 4 cut(s) 148, 183, 246, 401
BssT1I CCWWGG 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 453
Bst4CI ACNGT 3 cut(s) 318, 398, 418
Bst6I CTCTTC 1 cut(s) 477
BstC8I GCNNGC 1 cut(s) 145
BstDEI CTNAG 6 cut(s) 90, 366, 381, 389, 585, 606
BstDSI CCRYGG 1 cut(s) 246
BstF5I GGATG 2 cut(s) 177, 334
BstFNI CGCG 1 cut(s) 143
BstHHI GCGC 2 cut(s) 143, 161
BstMAI GTCTC 1 cut(s) 178
BstMWI GCNNNNNNNGC 1 cut(s) 19
BstNI CCWGG 1 cut(s) 453
BstSCI CCNGG 2 cut(s) 435, 451
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 4 cut(s) 25, 65, 396, 418
Bsu15I ATCGAT 1 cut(s) 114
Bsu36I CCTNAGG 1 cut(s) 389
BsuI GTATCC 1 cut(s) 76
BsuRI GGCC 3 cut(s) 86, 153, 201
BsuTUI ATCGAT 1 cut(s) 114
BtgI CCRYGG 1 cut(s) 246
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 2 cut(s) 177, 334
BtsI GCAGTG 1 cut(s) 154
BtsIMutI CAGTG 4 cut(s) 154, 314, 356, 494
Cac8I GCNNGC 1 cut(s) 145
CaiI CAGNNNCTG 2 cut(s) 316, 396
CfoI GCGC 2 cut(s) 143, 161
Cfr13I GGNCC 1 cut(s) 199
ClaI ATCGAT 1 cut(s) 114
CviAII CATG 3 cut(s) 190, 247, 374
DdeI CTNAG 6 cut(s) 90, 366, 381, 389, 585, 606
EaeI YGGCCR 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
Eco130I CCWWGG 1 cut(s) 246
Eco147I AGGCCT 1 cut(s) 86
Eco24I GRGCYC 1 cut(s) 593
Eco81I CCTNAGG 1 cut(s) 389
Eco88I CYCGRG 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 246
EcoT38I GRGCYC 1 cut(s) 593
ErhI CCWWGG 1 cut(s) 246
Esp3I CGTCTC 1 cut(s) 178
FaeI CATG 3 cut(s) 193, 250, 377
FaiI YATR 9 cut(s) 98, 128, 191, 248, 347, 354, 375, 414, 470
FaqI GGGAC 1 cut(s) 407
FatI CATG 3 cut(s) 189, 246, 373
Fnu4HI GCNGC 4 cut(s) 14, 79, 407, 410
FokI GGATG 2 cut(s) 164, 341
FriOI GRGCYC 1 cut(s) 593
Fsp4HI GCNGC 4 cut(s) 14, 79, 407, 410
GlaI GCGC 2 cut(s) 142, 160
GluI GCNGC 4 cut(s) 14, 79, 407, 410
GsaI CCCAGC 1 cut(s) 460
GsuI CTGGAG 1 cut(s) 380
HaeIII GGCC 3 cut(s) 86, 153, 201
HapII CCGG 2 cut(s) 17, 437
HhaI GCGC 2 cut(s) 143, 161
Hin1II CATG 3 cut(s) 193, 250, 377
Hin6I GCGC 2 cut(s) 141, 159
HinP1I GCGC 2 cut(s) 141, 159
HinfI GANTC 6 cut(s) 35, 41, 263, 541, 581, 610
HpaII CCGG 2 cut(s) 17, 437
HphI GGTGA 2 cut(s) 16, 635
Hpy188I TCNGA 5 cut(s) 40, 322, 369, 586, 607
Hpy188III TCNNGA 3 cut(s) 241, 427, 614
Hpy99I CGWCG 3 cut(s) 74, 107, 227
HpyAV CCTTC 2 cut(s) 436, 603
HpyCH4III ACNGT 3 cut(s) 318, 398, 418
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 1 cut(s) 412
HpyF10VI GCNNNNNNNGC 1 cut(s) 19
HpyF3I CTNAG 6 cut(s) 90, 366, 381, 389, 585, 606
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 3 cut(s) 193, 250, 377
HspAI GCGC 2 cut(s) 141, 159
LmnI GCTCC 2 cut(s) 19, 630
Lsp1109I GCAGC 4 cut(s) 25, 65, 396, 418
LweI GCATC 1 cut(s) 106
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 203
MboII GAAGA 3 cut(s) 19, 494, 536
MhlI GDGCHC 1 cut(s) 593
MluCI AATT 5 cut(s) 129, 284, 304, 475, 532
MlyI GAGTC 2 cut(s) 29, 35
MmeI TCCRAC 1 cut(s) 63
MseI TTAA 3 cut(s) 282, 333, 474
MslI CAYNNNNRTG 1 cut(s) 344
MspA1I CMGCKG 1 cut(s) 409
MspI CCGG 2 cut(s) 17, 437
MspR9I CCNGG 2 cut(s) 437, 453
MvaI CCWGG 1 cut(s) 453
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 1 cut(s) 19
NciI CCSGG 1 cut(s) 437
NcoI CCATGG 1 cut(s) 246
NlaIII CATG 3 cut(s) 193, 250, 377
NlaIV GGNNCC 3 cut(s) 21, 434, 632
NmeAIII GCCGAG 2 cut(s) 129, 382
NmuCI GTSAC 1 cut(s) 203
PceI AGGCCT 1 cut(s) 86
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 111
PfeI GAWTC 4 cut(s) 263, 541, 581, 610
PflFI GACNNNGTC 1 cut(s) 316
PflMI CCANNNNNTGG 1 cut(s) 247
PfoI TCCNGGA 1 cut(s) 435
PkrI GCNGC 4 cut(s) 15, 80, 408, 411
PleI GAGTC 2 cut(s) 29, 35
PpsI GAGTC 2 cut(s) 29, 35
Psp6I CCWGG 1 cut(s) 451
PspFI CCCAGC 1 cut(s) 456
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 3 cut(s) 21, 434, 632
PspPI GGNCC 1 cut(s) 199
PstNI CAGNNNCTG 2 cut(s) 316, 396
PsyI GACNNNGTC 1 cut(s) 316
PvuII CAGCTG 1 cut(s) 409
RseI CAYNNNNRTG 1 cut(s) 344
SaqAI TTAA 3 cut(s) 282, 333, 474
SatI GCNGC 4 cut(s) 14, 79, 407, 410
Sau96I GGNCC 1 cut(s) 199
SchI GAGTC 2 cut(s) 29, 35
ScrFI CCNGG 2 cut(s) 437, 453
SduI GDGCHC 1 cut(s) 593
SetI ASST 8 cut(s) 80, 189, 225, 339, 390, 411, 434, 641
SfaNI GCATC 1 cut(s) 106
SmiMI CAYNNNNRTG 1 cut(s) 344
Sse9I AATT 5 cut(s) 129, 284, 304, 475, 532
SseBI AGGCCT 1 cut(s) 86
SsiI CCGC 2 cut(s) 51, 509
StuI AGGCCT 1 cut(s) 86
StyD4I CCNGG 2 cut(s) 435, 451
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 3 cut(s) 318, 398, 418
TaiI ACGT 1 cut(s) 225
TaqI TCGA 3 cut(s) 72, 114, 536
TasI AATT 5 cut(s) 129, 284, 304, 475, 532
TfiI GAWTC 4 cut(s) 263, 541, 581, 610
Tru1I TTAA 3 cut(s) 282, 333, 474
Tru9I TTAA 3 cut(s) 282, 333, 474
TscAI CASTG 4 cut(s) 161, 321, 363, 501
TseFI GTSAC 1 cut(s) 203
TseI GCWGC 4 cut(s) 13, 78, 406, 409
Tsp45I GTSAC 1 cut(s) 203
TspDTI ATGAA 2 cut(s) 519, 590
TspGWI ACGGA 2 cut(s) 58, 219
TspRI CASTG 4 cut(s) 161, 321, 363, 501
Tth111I GACNNNGTC 1 cut(s) 316
Van91I CCANNNNNTGG 1 cut(s) 247
XapI RAATTY 2 cut(s) 284, 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.