Rroxscaffold_3G00247290

RNA-binding protein NOB1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001896
Physical Location & Seq
Forward (+)
40649652 .. 40650296
645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00247290.1

Sequence Viewer

Length: 486 bp
ATGGACCAGGTCCGTGACCCCGTTTCTCGCCATCGCCTTGCCCTCGTTCCCTTCCAAGTTCAGACCATGGAACCCTCCCCCGAATCCCTCAATAAAGTTTGCGAGGGCTACCGGGGATTTACGAAACTATCGGATGTGGATGTTAAGCTCATTGCTATGACTTATACATTAGAGGCTCGTATACATGGCACCGGCACCTCGTGGACTTTCCTCTCGCTAGTGCATACCGTCAATGTGAGGAGGTTGCCGGAGAAGGACTTGCTCGGGTGGGGAAATAATGTGCCTAATTTGGAAGAGTGGGAAGCGCCGGAGAACGAAGCGGAGGATAAGTTGAACCCCAGTTCGAGGATTCTTCCGTTGAAAGATATAAACTTGAATGTTATCGAGGATTCCGAGAGCCGTTCGTTGATAGTTCTGCAGTGGAAGTTAGGAGTGATGTTCATTCCGAGAATTAGGAGGGTGGTGAGGGCATTCGGGGAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
NOB1_Zn_bind PIN_6

Protein Analysis

161

Amino Acids

18.39

Weight (kDa)

5.95

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIN_6 PF17146 2 - 57 9.1e-08 PIN domain of ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 188, 194
AccI GTMKAC 1 cut(s) 181
AciI CCGC 1 cut(s) 320
AdeI CACNNNGTG 1 cut(s) 201
AfiI CCNNNNNNNGG 1 cut(s) 345
AgsI TTSAA 3 cut(s) 334, 361, 376
AjnI CCWGG 1 cut(s) 6
AluBI AGCT 1 cut(s) 148
AluI AGCT 1 cut(s) 148
Ama87I CYCGRG 1 cut(s) 263
AspLEI GCGC 1 cut(s) 307
AspS9I GGNCC 2 cut(s) 4, 10
AsuC2I CCSGG 1 cut(s) 113
AsuHPI GGTGA 1 cut(s) 475
AvaI CYCGRG 1 cut(s) 263
AvaII GGWCC 2 cut(s) 4, 10
BanI GGYRCC 2 cut(s) 188, 194
BauI CACGAG 1 cut(s) 199
BccI CCATC 1 cut(s) 39
BceAI ACGGC 1 cut(s) 384
BciT130I CCWGG 1 cut(s) 8
BcnI CCSGG 1 cut(s) 113
BfaI CTAG 1 cut(s) 218
BfmI CTRYAG 1 cut(s) 416
BfoI RGCGCY 1 cut(s) 308
Bme1390I CCNGG 2 cut(s) 8, 113
Bme18I GGWCC 2 cut(s) 4, 10
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 2 cut(s) 4, 10
BmiI GGNNCC 3 cut(s) 72, 190, 196
BmrFI CCNGG 2 cut(s) 8, 113
BmrI ACTGGG 1 cut(s) 333
BmuI ACTGGG 1 cut(s) 333
BpuMI CCSGG 1 cut(s) 113
BsaJI CCNNGG 2 cut(s) 66, 112
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 1 cut(s) 345
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 1 cut(s) 339
Bse3DI GCAATG 1 cut(s) 150
BseBI CCWGG 1 cut(s) 8
BseDI CCNNGG 2 cut(s) 66, 112
BseGI GGATG 2 cut(s) 139, 145
BseLI CCNNNNNNNGG 1 cut(s) 345
BseMI GCAATG 1 cut(s) 150
BseNI ACTGG 1 cut(s) 339
BseRI GAGGAG 1 cut(s) 253
BshNI GGYRCC 2 cut(s) 188, 194
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 4 cut(s) 112, 192, 248, 308
BslI CCNNNNNNNGG 1 cut(s) 345
BsmI GAATGC 1 cut(s) 470
BsoBI CYCGRG 1 cut(s) 263
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 1 cut(s) 320
BspLI GGNNCC 3 cut(s) 72, 190, 196
BspMAI CTGCAG 1 cut(s) 420
BspT107I GGYRCC 2 cut(s) 188, 194
BsrDI GCAATG 1 cut(s) 150
BsrFI RCCGGY 1 cut(s) 191
BsrI ACTGG 1 cut(s) 339
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 2 cut(s) 66, 112
BssNAI GTATAC 1 cut(s) 182
BssSI CACGAG 1 cut(s) 199
BssT1I CCWWGG 1 cut(s) 66
Bst1107I GTATAC 1 cut(s) 182
Bst2BI CACGAG 1 cut(s) 199
Bst2UI CCWGG 1 cut(s) 8
Bst4CI ACNGT 1 cut(s) 229
Bst6I CTCTTC 1 cut(s) 288
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 2 cut(s) 139, 145
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 1 cut(s) 307
BstNI CCWGG 1 cut(s) 8
BstSCI CCNGG 2 cut(s) 6, 111
BstSFI CTRYAG 1 cut(s) 416
BstZ17I GTATAC 1 cut(s) 182
BtgI CCRYGG 1 cut(s) 66
BtgZI GCGATG 1 cut(s) 17
BtsCI GGATG 2 cut(s) 139, 145
BtsI GCAGTG 1 cut(s) 425
BtsIMutI CAGTG 1 cut(s) 425
CfoI GCGC 1 cut(s) 307
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 2 cut(s) 4, 10
CsiI ACCWGGT 1 cut(s) 6
CviAII CATG 2 cut(s) 67, 185
CviJI RGCY 4 cut(s) 108, 148, 176, 399
CviKI_1 RGCY 4 cut(s) 108, 148, 176, 399
DraIII CACNNNGTG 1 cut(s) 201
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 66
Eco47I GGWCC 2 cut(s) 4, 10
Eco88I CYCGRG 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 6
EcoT14I CCWWGG 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 2 cut(s) 70, 188
FaiI YATR 8 cut(s) 68, 158, 165, 182, 186, 225, 368, 484
FatI CATG 2 cut(s) 66, 184
FblI GTMKAC 1 cut(s) 181
FokI GGATG 2 cut(s) 146, 152
FspBI CTAG 1 cut(s) 218
GlaI GCGC 1 cut(s) 306
HaeII RGCGCY 1 cut(s) 308
HapII CCGG 4 cut(s) 112, 192, 248, 308
HhaI GCGC 1 cut(s) 307
Hin1II CATG 2 cut(s) 70, 188
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HinfI GANTC 3 cut(s) 83, 349, 389
HpaII CCGG 4 cut(s) 112, 192, 248, 308
HphI GGTGA 1 cut(s) 475
Hpy166II GTNNAC 2 cut(s) 182, 204
Hpy188I TCNGA 4 cut(s) 63, 133, 394, 447
Hpy8I GTNNAC 2 cut(s) 182, 204
HpyAV CCTTC 2 cut(s) 61, 247
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4V TGCA 2 cut(s) 223, 418
Hsp92II CATG 2 cut(s) 70, 188
HspAI GCGC 1 cut(s) 305
LpnPI CCDG 6 cut(s) 20, 125, 205, 261, 321, 352
MabI ACCWGGT 1 cut(s) 6
MaeI CTAG 1 cut(s) 218
MaeIII GTNAC 1 cut(s) 14
MboII GAAGA 2 cut(s) 305, 344
MluCI AATT 2 cut(s) 286, 450
MseI TTAA 1 cut(s) 144
MslI CAYNNNNRTG 1 cut(s) 155
MspI CCGG 4 cut(s) 112, 192, 248, 308
MspR9I CCNGG 2 cut(s) 8, 113
Mva1269I GAATGC 1 cut(s) 470
MvaI CCWGG 1 cut(s) 8
NciI CCSGG 1 cut(s) 113
NcoI CCATGG 1 cut(s) 66
NlaIII CATG 2 cut(s) 70, 188
NlaIV GGNNCC 3 cut(s) 72, 190, 196
NmuCI GTSAC 1 cut(s) 14
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PctI GAATGC 1 cut(s) 470
PfeI GAWTC 3 cut(s) 83, 349, 389
PflFI GACNNNGTC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 6
PspGI CCWGG 1 cut(s) 6
PspN4I GGNNCC 3 cut(s) 72, 190, 196
PspPI GGNCC 2 cut(s) 4, 10
PstI CTGCAG 1 cut(s) 420
PsyI GACNNNGTC 1 cut(s) 8
RseI CAYNNNNRTG 1 cut(s) 155
SaqAI TTAA 1 cut(s) 144
Sau96I GGNCC 2 cut(s) 4, 10
ScrFI CCNGG 2 cut(s) 8, 113
SetI ASST 4 cut(s) 12, 150, 200, 245
SexAI ACCWGGT 1 cut(s) 6
SfcI CTRYAG 1 cut(s) 416
SinI GGWCC 2 cut(s) 4, 10
SmiMI CAYNNNNRTG 1 cut(s) 155
Sse9I AATT 2 cut(s) 286, 450
SsiI CCGC 1 cut(s) 320
SspMI CTAG 1 cut(s) 218
StyD4I CCNGG 2 cut(s) 6, 111
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 1 cut(s) 229
TaqI TCGA 2 cut(s) 344, 384
TasI AATT 2 cut(s) 286, 450
TfiI GAWTC 3 cut(s) 83, 349, 389
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TscAI CASTG 1 cut(s) 425
TseFI GTSAC 1 cut(s) 14
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 1 cut(s) 430
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 1 cut(s) 425
Tth111I GACNNNGTC 1 cut(s) 8
VpaK11BI GGWCC 2 cut(s) 4, 10
XmiI GTMKAC 1 cut(s) 181
XspI CTAG 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.